会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 1. 发明授权
    • Dispersion tool
    • 分散工具
    • US4745068A
    • 1988-05-17
    • US44150
    • 1987-04-30
    • Otis W. GodfreyWalter A. RaasJames R. Ayres
    • Otis W. GodfreyWalter A. RaasJames R. Ayres
    • B01F7/00B01F13/10B01F15/00C12M3/08C12M1/02
    • B01F15/00012B01F13/1041B01F7/00816B01F2013/108
    • A device for homogenizing a liquid containing a mixture of single cells and cell clusters is provided. The device includes a shroud for channeling the liquid and suspended particles. The shroud defines an inner bore that is formed to include an axial inlet opening and at least one spaced apart outlet opening. A homogenizing bit is provided that is disposed within the inner bore for dispersing the suspended particles in the liquid to form a homogenized substance. The bit includes a shear blade fixed to the bit at a location near the axial inlet opening, with the shear blade configured to cooperate with adjacent shear ports to shear a portion of the suspended particles. The bit also includes a spiral portion that is configured to draw the liquid and suspended particles axially into and through the inner bore from the inlet opening.
    • 提供了用于使含有单细胞和细胞簇的混合物的液体均质化的装置。 该装置包括用于引导液体和悬浮颗粒的护罩。 护罩限定内孔,其形成为包括轴向入口开口和至少一个间隔开的出口开口。 提供均质钻头,其设置在内孔内,用于将悬浮颗粒分散在液体中以形成均质物质。 该钻头包括固定在靠近轴向入口开口的位置处的钻头的剪切刀片,剪切刀片被配置成与相邻的剪切端口配合以剪切一部分悬浮颗粒。 钻头还包括螺旋部分,其被构造成从入口开口将液体和悬浮颗粒轴向地吸入和穿过内孔。
    • 8. 发明授权
    • Recombinant DNA expression vectors and DNA compounds which encode
isopenicillin N synthetase
    • 编码异青霉素N合成酶的重组DNA表达载体和DNA化合物
    • US4885251A
    • 1989-12-05
    • US895008
    • 1986-08-08
    • Thomas D. IngoliaStephen W. QueenerSuellen M. SamsonPaul L. SkatrudOtis W. Godfrey
    • Thomas D. IngoliaStephen W. QueenerSuellen M. SamsonPaul L. SkatrudOtis W. Godfrey
    • C12N15/52C12N9/00C12N15/69C12N15/70C12N15/74C12N15/80
    • C12N15/80C12N15/69C12N9/93
    • The present invention comprises novel DNA compounds that encode isopenicillin N synthetase and also comprises related methods, transformants, and polypeptides. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcriptional and translational activating sequence, was isolated from Cephalosporium acremonium and cloned into an E. coli cloning vector. The isopenicillin N synthetase-encoding DNA has been used to construct novel E. coli expression vectors that drive expression of a stable, active, and novel isopenicillin N synthetase in E. coli. The intact C. acremonium isopenicillin N synthetase-encoding DNA and associated transcriptional and translational activating sequence have also been used to construct C. acremonium expression vectors that drive expression of the isopenicillin N synthetase in C. acremonium. The C. acremonium transcriptional and translational activating sequence has further been fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto C. acremonium expression vectors. Useful derivatives of the novel compounds and vectors are also described.
    • 本发明包括编码异青霉素N合成酶的新型DNA化合物,还包括相关方法,转化体和多肽。 编码新的异青霉素N合成酶DNA,连同其相关的转录和翻译活化序列,从头孢霉头孢菌分离并克隆到大肠杆菌克隆载体中。 编码异青霉素N合成酶的DNA已经用于构建新的大肠杆菌表达载体,其在大肠杆菌中驱动稳定的,活性的和新的异青霉素N合成酶的表达。 编码完整的顶头孢霉素异青霉素N合成酶的DNA和相关的转录和翻译活化序列也被用于构建顶头孢霉表达载体,其驱动顶头孢霉中异青霉素N合成酶的表达。 顶头孢霉转录和翻译激活序列进一步融合到编码潮霉素磷酸转移酶的DNA片段并置于顶头孢霉表达载体上。 还描述了新化合物和载体的有用衍生物。