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1. 发明授权

US11426724B2 Microfluidic cellular device and methods of use thereof 有权
公开(公告)号：US11426724B2
公开(公告)日：2022-08-30
申请号：US16244920
申请日：2019-01-10
申请人： NanoCav, LLC
发明人： Yu-Chun Kung
IPC分类号： B01L3/00 , G01N1/40 , G01N15/02
摘要： Systems and methods for using microfluidic devices to concentrate cells, to perform buffer changes, to sort cells based on size, and/or to isolate particular types of cells in a rapid manner, are presented. Cells flow into a matrix of posts, wherein the posts are distributed along diagonal lines in the chamber. The cells are deflected in a lateral manner, towards a side of a chamber and are collected upon exiting the chamber.

2. 发明公开

US20230304043A1 Devices And Methods For Mitochondria Replacement And For Generating Cellular Therapeutics 审中-公开
公开(公告)号：US20230304043A1
公开(公告)日：2023-09-28
申请号：US18117915
申请日：2023-03-06
申请人： NanoCav, LLC
发明人： Ting-Hsiang Sherry WU , Artin MEHRABI , Jon Thomas VAN LEW
IPC分类号： C12N15/87 , B01L3/00 , C12N5/0775
CPC分类号： C12N15/87 , B01L3/502715 , B01L3/50273 , C12N5/0662 , B01L2300/041 , B01L2300/06 , B01L2400/0481 , C12N2506/45
摘要： Devices and methods are presented for delivery of various macrostructures into cells, such as depleted cells as well as methods of generating engineered cells using said devices and methods. Cells are placed on a porous membrane. A force is applied by a configurable actuator to a deformable fluid reservoir that generates an applied pressure to macrostructures in a solution, causing the macrostructures to pass through the porous membrane and triggering uptake of at least some of the macrostructures into the cells to form transfected cells. Said devices and methods may be used in a process to replace defective endogenous mtDNA with corrected mtDNA to generate cellular-based therapeutics for administration to a patient. The customizable actuator may be configured with parameters to optimize efficiency for a given cell type and material to be transfected. Machine learning techniques also may be utilized to optimize transfection parameters.

3. 发明授权

US11639510B2 Devices and methods for mitochondria replacement and for generating cellular therapeutics 有权
公开(公告)号：US11639510B2
公开(公告)日：2023-05-02
申请号：US16517017
申请日：2019-07-19
申请人： NanoCav, LLC
发明人： Ting-Hsiang Sherry Wu , Artin Mehrabi , Jon Thomas Van Lew
IPC分类号： C12N15/87 , C12N5/0775 , B01L3/00
摘要： Devices and methods are presented for delivery of various macrostructures into cells, such as depleted cells as well as methods of generating engineered cells using said devices and methods. Cells are placed on a porous membrane. A force is applied by a configurable actuator to a deformable fluid reservoir that generates an applied pressure to macrostructures in a solution, causing the macrostructures to pass through the porous membrane and triggering uptake of at least some of the macrostructures into the cells to form transfected cells. Said devices and methods may be used in a process to replace defective endogenous mtDNA with corrected mtDNA to generate cellular-based therapeutics for administration to a patient. The customizable actuator may be configured with parameters to optimize efficiency for a given cell type and material to be transfected. Machine learning techniques also may be utilized to optimize transfection parameters.

4. 发明公开

US20240075267A1 Microneedle Array Device, Methods Of Manufacturing And Use Thereof 审中-公开
公开(公告)号：US20240075267A1
公开(公告)日：2024-03-07
申请号：US18388356
申请日：2023-11-09
申请人： NanoCav, LLC
发明人： Chihwei CHANG
IPC分类号： A61M37/00 , A61L31/04 , A61L31/10 , A61L31/16 , B29C33/56 , B29C41/04 , B29C41/22 , B33Y10/00 , B33Y80/00
CPC分类号： A61M37/0015 , A61L31/041 , A61L31/10 , A61L31/16 , A61M37/0076 , B29C33/56 , B29C41/04 , B29C41/22 , B33Y10/00 , B33Y80/00 , A61M2037/0023 , A61M2037/0053 , A61M2037/0061 , B29K2001/08
摘要： Systems and methods for using microneedle arrays to deliver bioactive compounds are presented. In general, the microeconomic array comprises at least three layers: a base layer, a separation layer, and a bioactive layer, wherein the separation layer is situated between the base layer and the bioactive layer. Upon exposure to physiological conditions, the separation layer dissolves and/or disperses, allowing the base layer to be removed while the bioactive layer remains embedded in the outer surface of the skin.

5. 发明授权

US11377652B2 Micro flow-through electroporation devices and methods of cell transfection 有权
公开(公告)号：US11377652B2
公开(公告)日：2022-07-05
申请号：US16506190
申请日：2019-07-09
申请人： NanoCav, LLC
发明人： Chih-Wei Chang
IPC分类号： C12N15/87 , C12N13/00
摘要： Systems and methods are provided for transfecting cells, such as mammalian cells and nonmammalian cells, using an electroporation apparatus having an electroporation chamber including an upper micromesh electrode, a lower micromesh electrode and a path defined in the electroporation chamber. The electroporation apparatus includes a first input allowing passage of cells into the electroporation chamber and a first output allowing passage of electroporated cells from the electroporation chamber. The first input and the first output are separated by an offset distance.

6. 发明申请

US20210348155A1 Micro Flow-Through Electroporation Devices And Methods Of Cell Transfection 有权
公开(公告)号：US20210348155A1
公开(公告)日：2021-11-11
申请号：US17380799
申请日：2021-07-20
申请人： NanoCav, LLC
发明人： Chih-Wei CHANG
IPC分类号： C12N13/00 , C12N15/87
摘要： Systems and methods are provided for transfecting cells, such as mammalian cells and nonmammalian cells, using an electroporation apparatus having an electroporation chamber including an upper micromesh electrode, a lower micromesh electrode and a path defined in the electroporation chamber. The electroporation apparatus includes a first input allowing passage of cells into the electroporation chamber and a first output allowing passage of electroporated cells from the electroporation chamber. The first input and the first output are separated by an offset distance.

7. 发明授权

US10760040B1 Mechanical transfection devices and methods 有权
公开(公告)号：US10760040B1
公开(公告)日：2020-09-01
申请号：US14754625
申请日：2015-06-29
申请人： NanoCav, LLC
发明人： Ting Wu
IPC分类号： C12M1/12 , C12M1/00 , C12M1/34
摘要： A transfection device suitable for delivery of various macrostructures (e.g., mitochondria, bacteria, liposomes) is described and uses mechanical force to thereby induce active endocytosis in a target cell. Contemplated devices are able to achieve high throughput of transfected cells that remain viable and are capable of producing colonies.

8. 发明申请

US20200009259A1 Methods Of Transfection For Large Cargo Using Poly(Beta-Amino Esters) 审中-公开
公开(公告)号：US20200009259A1
公开(公告)日：2020-01-09
申请号：US16506478
申请日：2019-07-09
申请人： NanoCav, LLC
发明人： Ting-Hsiang Sherry WU , Wade Nichols RICHARDSON
IPC分类号： A61K47/58 , A61K39/235 , A61K35/00 , C12N7/00
摘要： Methods are provided for transfecting cells with large cargo using a poly(beta-amino ester) (PBAE) molecule, and achieving high efficiency and viability. A method is provided of transfecting cells with a cargo, by forming a complex of the cargo with a (PBAE) molecule, mixing the complex with a first buffer and contacting the complex with the cells, wherein the cargo has a dimension of at least 0.1 μm. The PBAE molecule may be formed by reacting an amine with a di(acrylate ester). In some aspects, the PBAE molecule is poly(1,4-butanediol diacrylate-co-4-amino-1-butanol). In some aspects, the PBAE molecule is capped with 1-(3-aminopropyl)-4-methylpiperazine.

9. 外观设计

USD1016324S1 Biological cell processing chip 有权
公开(公告)号：USD1016324S1
公开(公告)日：2024-02-27
申请号：US29812292
申请日：2021-10-20
申请人： NanoCav, LLC
设计人： Chihwei Chang , Wade Richardson , Artin Mehrabi
摘要： FIG. 1 is an isometric view of a biological cell processing chip in accordance with our new design;
FIG. 2 is a front elevation view thereof;
FIG. 3 is a rear elevation view thereof;
FIG. 4 is a right side elevation view thereof;
FIG. 5 is a left side elevation view thereof;
FIG. 6 is a top plan view thereof;
FIG. 7 is a bottom plan view thereof;
FIG. 8 is an isometric view of the biological cell processing chip of FIGS. 1-7 in a state of use;
FIG. 9 is an isometric view of another embodiment of a biological cell processing chip in accordance with our new design;
FIG. 10 is a front elevation view thereof;
FIG. 11 is a rear elevation view thereof;
FIG. 12 is a right side elevation view thereof;
FIG. 13 is a left side elevation view thereof;
FIG. 14 is a top plan view thereof;
FIG. 15 is a bottom plan view thereof; and,
FIG. 16 is an isometric view of the biological cell processing chip of FIGS. 9-15 in a state of use.
The broken lines in FIGS. 1-7 and 9-15, the areas within them, and the unshaded areas bounded by broken lines and solid lines depict portions of the biological cell processing chip that form no part of the claimed design. The additional broken lines shown in FIGS. 8 and 16 depict environmental subject matter and form no part of the claimed design.

10. 发明授权

US11549089B2 Mechanical transfection devices and methods 有权
公开(公告)号：US11549089B2
公开(公告)日：2023-01-10
申请号：US16939728
申请日：2020-07-27
申请人： NanoCav, LLC
发明人： Ting Wu
IPC分类号： C12M1/12 , C12M1/00 , C12M1/34
摘要： A transfection device suitable for delivery of various macrostructures (e.g., mitochondria, bacteria, liposomes) is described and uses mechanical force to thereby induce active endocytosis in a target cell. Contemplated devices are able to achieve high throughput of transfected cells that remain viable and are capable of producing colonies.

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