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1. 发明申请

US20110081403A1 HISTONE OCTAMERS FOR INCREASED NUCLEIC ACID TRANSFER 审中-公开
公开(公告)号：US20110081403A1
公开(公告)日：2011-04-07
申请号：US12887666
申请日：2010-09-22
申请人： Nancy Smyth Templeton
发明人： Nancy Smyth Templeton
IPC分类号： A61K9/127 , A61K38/16 , A61P35/00
CPC分类号： A61K38/1709 , A61K9/1273 , A61K48/00 , A61K48/0025 , C12N2830/60
摘要： The present invention provides reconstituted histone octamers with multiple modifications (e.g. acetylation of all histones and trimethylation of histone H3K4) assembled onto plasmids for increased transcription post-transfection using our unique bi-lamellar invaginated liposomes (BIVs) to more effectively recruit the transcriptional machinery of human cancer cells post-transfection and substantially increase the production of therapeutic gene products.
摘要翻译：本发明提供了组装到质粒上的多种修饰（例如组蛋白的乙酰化和组蛋白H3K4的三甲基化）的复组组蛋白八聚体，用于使用我们独特的双片层入侵的脂质体（BIV）以更有效地招募转录机制转染后的人类癌细胞，并大大增加治疗性基因产物的产生。

2. 发明申请

US20110020924A1 HIGHLY PURE PLASMID DNA PREPARATIONS 审中-公开
标题翻译：高纯度PLASMID DNA制备
公开(公告)号：US20110020924A1
公开(公告)日：2011-01-27
申请号：US12433645
申请日：2009-04-30
申请人： Nancy Smyth Templeton
发明人： Nancy Smyth Templeton
IPC分类号： C12N15/63
CPC分类号： C12N15/1017 , B01D15/327 , B01D15/362 , B01D15/363 , B01D15/3804 , C07H21/04 , C12N9/2402 , C12N15/1003 , C12N15/101 , C12N15/63
摘要： The present disclosure generally relates to highly pure plasmid compositions having low, or undetectable, levels of colanic acid and other contaminants. The compositions described herein have a range of uses, including diverse applications in the field of bioterrorism, environmental science, food science, forensics, molecular biology, and health and medicine.
摘要翻译：本公开内容通常涉及具有低或不可检测的荚果酸和其它污染物水平的高纯度质粒组合物。本文描述的组合物具有一系列用途，包括生物恐怖主义，环境科学，食品科学，法医学，分子生物学以及健康和医学领域的各种应用。

3. 发明申请

US20090275088A1 PROCESSES FOR THE PREPARATION OF HIGHLY PURE PLASMID COMPOSITIONS 有权
标题翻译：制备高纯度PLASMID组合物的方法
公开(公告)号：US20090275088A1
公开(公告)日：2009-11-05
申请号：US12433674
申请日：2009-04-30
申请人： Nancy Smyth Templeton
发明人： Nancy Smyth Templeton
IPC分类号： C12N15/64
CPC分类号： C12N15/1017 , B01D15/327 , B01D15/362 , B01D15/363 , B01D15/3804 , C07H21/04 , C12N9/2402 , C12N15/1003 , C12N15/101 , C12N15/63
摘要： The present disclosure generally relates to processes for preparing highly pure plasmid compositions. The processes generally involve treating a composition comprising plasmid DNA with a polypeptide to digest colanic acid. The treated plasmid DNA is then separated from the treated composition.
摘要翻译：本公开一般涉及制备高纯度质粒组合物的方法。该方法通常涉及用多肽处理包含质粒DNA的组合物以消化荚膜异多糖。然后将经处理的质粒DNA与经处理的组合物分离。

4. 发明授权

US07037520B2 Reversible masking of liposomal complexes for targeted delivery 失效
公开(公告)号：US07037520B2
公开(公告)日：2006-05-02
申请号：US10393101
申请日：2003-03-20
申请人： Nancy Smyth Templeton
发明人： Nancy Smyth Templeton
IPC分类号： C12N15/87 , A61K9/127
CPC分类号： C12N15/88 , A61K9/1271 , C12N2810/40
摘要： The present invention is a composition and process for avoiding non-specific uptake of targeted liposomal complexes in the lung and other highly vascular issues. The reversible masking of liposomal complexes allows increased delivery of nucleic acid molecules to target cells or tissues.

5. 发明授权

US08647857B2 Processes for the digestion of colanic acid 失效
标题翻译：荚果酸消化过程
公开(公告)号：US08647857B2
公开(公告)日：2014-02-11
申请号：US12433701
申请日：2009-04-30
申请人： Nancy Smyth Templeton
发明人： Nancy Smyth Templeton
IPC分类号： C12P41/00
CPC分类号： C12N15/1017 , B01D15/327 , B01D15/362 , B01D15/363 , B01D15/3804 , C07H21/04 , C12N9/2402 , C12N15/1003 , C12N15/101 , C12N15/63
摘要： The present disclosure generally relates to processes employing polypeptides having colanic acid-degrading activity. The processes generally involve contacting a biological material with a polypeptide capable of digesting colanic acid. Additional process steps, such as chromatographic separation steps, are also described.
摘要翻译：本公开通常涉及使用具有col酸降解活性的多肽的方法。所述方法通常包括使生物材料与能够消化col酸的多肽接触。还描述了另外的工艺步骤，例如色谱分离步骤。

6. 发明授权

US07001614B2 Liposome complexes for increased systemic delivery 有权
标题翻译：用于增加系统递送的脂质体复合物
公开(公告)号：US07001614B2
公开(公告)日：2006-02-21
申请号：US10825803
申请日：2004-04-15
申请人： Nancy Smyth-Templeton , George N. Pavlakis
发明人： Nancy Smyth-Templeton , George N. Pavlakis
IPC分类号： A61K9/127
CPC分类号： A61K48/00 , A61K9/1272 , A61K47/6911 , C12N15/88
摘要： Highly efficient cationic liposomes have been developed as an improved delivery system for biologically-active reagents. A novel structure, the sandwich liposome, is formed and comprises one or more biologically active agents internalized between two bilomellar liposomes. This structure protects the incoming agent and accounts for the high efficiency of in vivo delivery and for the broad tissue distribution of the sandwich liposome complexes.These novel liposomes are also highly efficient carriers of nucleic acids. By using extruded DOTAP:cholesterol liposomes to form complexes with DNA encoding specific proteins, expression has been improved dramatically. Highest expression was achieved in the lung, while increased expression was detected in several organs and tissues.
摘要翻译：已经开发了高效的阳离子脂质体作为用于生物活性试剂的改进的递送系统。形成了一种新颖的结构，即夹心脂质体，并且包含一种或多种在两个生物体脂质体之间内在的生物活性剂。这种结构保护进入的药剂并且考虑到体内递送的高效率和夹心脂质体复合物的广泛的组织分布。

7. 发明授权

US06770291B2 Liposome complexes for increased systemic delivery 有权
标题翻译：用于增加系统递送的脂质体复合物
公开(公告)号：US06770291B2
公开(公告)日：2004-08-03
申请号：US09991028
申请日：2001-11-20
申请人： Nancy Smyth-Templeton , George N. Pavlakis
发明人： Nancy Smyth-Templeton , George N. Pavlakis
IPC分类号： A61K9127
CPC分类号： C12N15/88 , A61K48/00
摘要： Highly efficient cationic liposomes have been developed as an improved delivery system for biologically-active reagents. A novel structure, the sandwich liposome, is formed and comprises one or more biologically active agents internalized between two bilomellar liposomes. This structure protects the incoming agent and accounts for the high efficiency of in vivo delivery and for the broad tissue distribution of the sandwich liposome complexes. These novel liposomes are also highly efficient carriers of nucleic acids. By using extruded DOTAP:cholesterol liposomes to form complexes with DNA encoding specific proteins, expression has been improved dramatically. Highest expression was achieved in the lung, while increased expression was detected in several organs and tissues.
摘要翻译：已经开发了高效的阳离子脂质体作为用于生物活性试剂的改进的递送系统。形成了一种新颖的结构，即夹心脂质体，并且包含一种或多种在两个生物体脂质体之间内在的生物活性剂。这种结构保护了进入的药剂并且考虑到体内递送的高效率和夹心脂质体复合物的广泛的组织分布。这些新的脂质体也是核酸的高效载体。通过使用挤出的DOTAP：胆固醇脂质体与编码特定蛋白质的DNA形成复合物，表达显着改善。在肺中达到最高表达，而在几个器官和组织中检测到表达增加。

8. 发明授权

US08969068B2 Processes for the preparation of highly pure plasmid compositions by enzymatic digestion of colanic acid 有权
标题翻译：通过酶解消化col酸制备高纯度质粒组合物的方法
公开(公告)号：US08969068B2
公开(公告)日：2015-03-03
申请号：US12433674
申请日：2009-04-30
申请人： Nancy Smyth Templeton
发明人： Nancy Smyth Templeton
IPC分类号： C12P1/00 , C12S3/00 , C12N9/24 , C12N15/10 , C07H21/04 , C12N15/63
CPC分类号： C12N15/1017 , B01D15/327 , B01D15/362 , B01D15/363 , B01D15/3804 , C07H21/04 , C12N9/2402 , C12N15/1003 , C12N15/101 , C12N15/63
摘要： A process for the purification of plasmid DNA, the process comprising treating an aqueous composition containing plasmid DNA with a polypeptide obtained from an NST1 phage to digest colanic acid and separating the plasmid DNA from the treated aqueous composition.
摘要翻译：一种用于纯化质粒DNA的方法，所述方法包括用从NST1噬菌体获得的多肽处理含有质粒DNA的含水组合物以消化colanic酸，并从经处理的水性组合物中分离质粒DNA。

9. 发明授权

US08460908B2 Polypeptides having colanic acid-degrading activity 有权
标题翻译：具有荚果酸降解活性的多肽
公开(公告)号：US08460908B2
公开(公告)日：2013-06-11
申请号：US12433691
申请日：2009-04-30
申请人： Nancy Smyth Templeton
发明人： Nancy Smyth Templeton
IPC分类号： C12N9/00
CPC分类号： C12N15/1017 , B01D15/327 , B01D15/362 , B01D15/363 , B01D15/3804 , C07H21/04 , C12N9/2402 , C12N15/1003 , C12N15/101 , C12N15/63
摘要： The present disclosure generally relates to polypeptides having colanic acid-degrading activity and methods of using the same. Polynucleotides encoding such polypeptides are also described. The polypeptides may be used, for example, in processes for degrading colanic acid, processes for the removal of endotoxins from biological samples, and processes for purifying plasmid DNA.
摘要翻译：本公开内容一般涉及具有col酸降解活性的多肽及其使用方法。还描述了编码这种多肽的多核苷酸。多肽可用于例如降解荚膜氨酸的方法，从生物样品中去除内毒素的方法，以及纯化质粒DNA的方法。

10. 发明申请

US20130129813A1 TARGETED DELIVERY USING TISSUE-SPECIFIC PEPTIDOMIMETIC LIGANDS 有权
标题翻译：使用组织特异性配体配体进行定向递送
公开(公告)号：US20130129813A1
公开(公告)日：2013-05-23
申请号：US13664288
申请日：2012-10-30
申请人： Nancy Smyth Templeton
发明人： Nancy Smyth Templeton
IPC分类号： C07D403/14 , C12Q1/02 , A61K47/22
CPC分类号： C07D403/14 , A61K31/711 , A61K47/22 , A61K47/64 , C12Q1/025 , G01N33/6845
摘要： Compositions and methods for tissue-specific targeted delivery of therapeutic agents through the use of tissue-specific peptidomimetic ligands are disclosed herein. The ligand comprises a composition of formula A-scaffold-A′ and one or more hydrophobic anchors covalently linked to the scaffold. The A and A′ compounds linked to the scaffold comprise monovalent peptidomimetic compounds wherein each monovalent peptidomimetic compound is selected from the group consisting of fragments IKs, GKs, IDs, GSs, GTs, VSs, TKs, KTs, ARs, KIs, KEs, AEs, GRs, YSs, IRs, and morpholino.
摘要翻译：本文公开了通过使用组织特异性拟肽配体对组织特异性靶向递送治疗剂的组合物和方法。配体包含式A-支架-A'的组合物和与支架共价连接的一个或多个疏水锚。与支架连接的A和A'化合物包含单价拟肽化合物，其中每个单价拟肽化合物选自片段IK，GK，ID，GS，GT，VS，TK，KT，AR，KI，KEs，AE ，GRs，YSs，IRs和吗啉代。

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