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    • 3. 发明申请
    • PROTEASE DETECTION MATERIAL, SET OF PROTEASE DETECTION MATERIALS, AND METHOD FOR MEASURING PROTEASE
    • 蛋白质检测材料,蛋白酶检测材料组和测定蛋白质的方法
    • US20090246815A1
    • 2009-10-01
    • US12414325
    • 2009-03-30
    • Sadanobu SHUTOKatsuhisa OHZEKIMikinaga MORI
    • Sadanobu SHUTOKatsuhisa OHZEKIMikinaga MORI
    • C12Q1/37
    • C12Q1/37G01N2333/8146G01N2333/96486
    • The present invention provides a protease detection material having, on a support, at least a layer containing a dye precursor and a layer which contains a protease substrate and is disposed on the same side of the support as the layer containing the dye precursor and farther from the support than the layer containing the dye precursor, wherein the layer containing the protease substrate further contains a development center-forming substance. Further, a set of protease detection materials and a method for measuring protease using the above-described protease detection material are provided. A protease detection material, a set of protease detection materials, and a method of measuring protease which allow protease activity to be measured promptly with high sensitivity, and that allow, at the same time, morphological observation of tissues and cells on a thin film to be performed easily.
    • 本发明提供一种蛋白酶检测材料,其在载体上至少含有染料前体和含有蛋白酶底物的层,并且与含有染料前体的层位于载体的同一侧, 比含有染料前体的层的载体,其中含有蛋白酶底物的层还含有显影中心形成物质。 此外,提供一组蛋白酶检测材料和使用上述蛋白酶检测材料测定蛋白酶的方法。 一种蛋白酶检测材料,一组蛋白酶检测材料,以及测定蛋白酶的方法,该蛋白酶允许以高灵敏度迅速测定蛋白酶活性,同时允许同时对薄膜上的组织和细胞进行形态学观察 容易执行
    • 5. 发明授权
    • Assay method
    • 测定方法
    • US08663910B2
    • 2014-03-04
    • US12562885
    • 2009-09-18
    • Mikinaga MoriJunichi KatadaHiroyuki ChikuTakayoshi Oyamada
    • Mikinaga MoriJunichi KatadaHiroyuki ChikuTakayoshi Oyamada
    • C12Q1/00
    • G01N33/54366
    • A plurality of kinds of liquids, which are of at least three kinds, containing (a) a test body solution containing at least one kind of an analyte, and (b) at least two kinds of liquids selected from the group consisting of a reagent solution, an amplifying solution, and a detecting solution, are fed to a detection site containing a specific binding substance with respect to the analyte. A qualitative analysis or a quantitative analysis of the analyte contained in the test body solution is thereby performed. Directions of liquid feeding of all of the plurality of the kinds of the liquids vary from one another, and the plurality of the kinds of the liquids are caused to intersect with one another at the detection site.
    • 至少三种的多种液体,其含有(a)含有至少一种分析物的测试体溶液,和(b)至少两种选自试剂 溶液,放大溶液和检测溶液相对于分析物进料到含有特异性结合物质的检测部位。 因此,进行了测试体溶液中包含的分析物的定性分析或定量分析。 所有多种液体的液体供给方向彼此不同,并且使多种液体在检测部位彼此相交。
    • 7. 发明授权
    • Method and device for assay
    • 测定方法和装置
    • US08802426B2
    • 2014-08-12
    • US13262526
    • 2010-03-30
    • Mikinaga MoriJunichi KatadaTakayoshi OyamadaHideyuki KarakiMasayasu KonishiHiroki Terada
    • Mikinaga MoriJunichi KatadaTakayoshi OyamadaHideyuki KarakiMasayasu KonishiHiroki Terada
    • B01L3/00G01N33/543
    • G01N33/54386
    • A device for assay can evenly develop solution, and performs highly accurate and sensitive measurement. A first device part (10) maintains a second insoluble carrier (12) and a third insoluble carrier (13) in such a manner that they overlap with each other at a detection portion (14) of a first insoluble carrier (11). These three carriers (11), (12) and (13) are housed not in contact with each other. A pressing unit (18) having a pressing surface (18a) that is parallel to the detection portion (14) is provided on an inner surface of the second device part (20) facing the detection portion (14). The pressing surface (18a) is displaced by being pressed toward the detection portion (14), and presses, from the upper side of the first insoluble carrier (11), the second insoluble carrier (12) and the third insoluble carrier (13) onto the first insoluble carrier (11). The first device part (10) and the second device part (20) are joined together.
    • 用于测定的装置可以均匀地开发溶液,并进行高度精确和灵敏的测量。 第一装置部分(10)以这样的方式保持第二不溶性载体(12)和第三不溶性载体(13),使得它们在第一不溶性载体(11)的检测部分(14)处彼此重叠。 这三个载体(11),(12)和(13)彼此不接触地容纳。 在与检测部(14)相对的第二装置部(20)的内表面上设置有具有与检测部(14)平行的按压面(18a)的按压部(18)。 按压面(18a)通过朝向检测部(14)被按压而移位,从第一不溶性载体(11)的上侧按压第二不溶性载体(12)和第三不溶性载体(13) 到第一不溶性载体(11)上。 第一装置部件(10)和第二装置部件(20)连接在一起。
    • 8. 发明申请
    • METHOD AND DEVICE FOR ASSAY
    • 用于测定的方法和装置
    • US20120058465A1
    • 2012-03-08
    • US13262526
    • 2010-03-30
    • Mikinaga MoriJunichi KatadaTakayoshi OyamadaHideyuki KarakiMasayasu KonishiHiroki Terada
    • Mikinaga MoriJunichi KatadaTakayoshi OyamadaHideyuki KarakiMasayasu KonishiHiroki Terada
    • C12Q1/70C12M1/34
    • G01N33/54386
    • A device for assay can evenly develop solution, and performs highly accurate and sensitive measurement. A first device part (10) maintains a second insoluble carrier (12) and a third insoluble carrier (13) in such a manner that they overlap with each other at a detection portion (14) of a first insoluble carrier (11). These three carriers (11), (12) and (13) are housed not in contact with each other. A pressing unit (18) having a pressing surface (18a) that is parallel to the detection portion (14) is provided on an inner surface of the second device part (20) facing the detection portion (14). The pressing surface (18a) is displaced by being pressed toward the detection portion (14), and presses, from the upper side of the first insoluble carrier (11), the second insoluble carrier (12) and the third insoluble carrier (13) onto the first insoluble carrier (11). The first device part (10) and the second device part (20) are joined together.
    • 用于测定的装置可以均匀地开发溶液,并进行高度精确和灵敏的测量。 第一装置部分(10)以这样的方式保持第二不溶性载体(12)和第三不溶性载体(13),使得它们在第一不溶性载体(11)的检测部分(14)处彼此重叠。 这三个载体(11),(12)和(13)彼此不接触地容纳。 在与检测部(14)相对的第二装置部(20)的内表面上设置有具有与检测部(14)平行的按压面(18a)的按压部(18)。 按压面(18a)通过朝向检测部(14)被按压而移位,从第一不溶性载体(11)的上侧按压第二不溶性载体(12)和第三不溶性载体(13) 到第一不溶性载体(11)上。 第一装置部件(10)和第二装置部件(20)连接在一起。