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1. 发明授权

US08039256B2 Culturing method of mesenchymal stem cell 有权
标题翻译：间充质干细胞的培养方法
公开(公告)号：US08039256B2
公开(公告)日：2011-10-18
申请号：US12892223
申请日：2010-09-28
申请人： Yuhiro Sakai , Katsuyuki Yamanaka , Mika Takeda , Tomohisa Okura , Koichiro Tsuji
发明人： Yuhiro Sakai , Katsuyuki Yamanaka , Mika Takeda , Tomohisa Okura , Koichiro Tsuji
IPC分类号： C12N5/00 , C12N5/07 , C12N5/10 , C12N5/071
CPC分类号： C12N5/0663 , C12N2500/50
摘要： To efficiently select and proliferate the mesenchymal stem cells without necessity of an exclusive separating device and a complicated separating operation, mesenchymal stem cells are cultured by seeding at least one of a bone marrow solution, an umbilical cord blood, a peripheral blood, a synovial membrane and an amniotic membrane in a liquid culture medium which is filled in a vessel, includes water as its main components and having a specific gravity between 1.06 and 1.10 at 37° C., and making a culture at a temperature 37±2° C. on a ceiling side surface of the vessel, preferably the specific gravity being regulated by use of at least one selected from silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound including a triiodo aromatic ring.
摘要翻译：为了有效地选择和增殖间充质干细胞而不需要专门的分离装置和复杂的分离操作，通过种植骨髓溶液，脐带血，外周血，滑膜中的至少一种来培养间充质干细胞和填充在容器中的液体培养基中的羊膜包含水作为主要成分，在37℃下的比重为1.06〜1.10，培养温度为37±2℃。在容器的顶棚侧表面上，优选使用选自由聚乙烯吡咯烷酮涂覆的二氧化硅细粉末，蔗糖和表氯醇的水溶性共聚物和包含三碘芳环的水溶性化合物中的至少一种来调节比重。

2. 发明申请

US20110076770A1 CULTURING METHOD OF MESENCHYMAL STEM CELL 有权
标题翻译：细胞干细胞的培养方法
公开(公告)号：US20110076770A1
公开(公告)日：2011-03-31
申请号：US12892223
申请日：2010-09-28
申请人： Yuhiro SAKAI , Katsuyuki Yamanaka , Mika Takeda , Tomohisa Okura , Koichiro Tsuji
发明人： Yuhiro SAKAI , Katsuyuki Yamanaka , Mika Takeda , Tomohisa Okura , Koichiro Tsuji
IPC分类号： C12N5/077
CPC分类号： C12N5/0663 , C12N2500/50
摘要： To efficiently select and proliferate the mesenchymal stem cells without necessity of an exclusive separating device and a complicated separating operation, mesenchymal stem cells are cultured by seeding at least one of a bone marrow solution, an umbilical cord blood, a peripheral blood, a synovial membrane and an amniotic membrane in a liquid culture medium which is filled in a vessel, includes water as its main components and having a specific gravity between 1.06 and 1.10 g/ml at 37° C., and making a culture at a temperature 37±2° C. on a ceiling side surface of the vessel, preferably the specific gravity being regulated by use of at least one selected from silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound including a triiodo aromatic ring.
摘要翻译：为了有效地选择和增殖间充质干细胞而不需要专门的分离装置和复杂的分离操作，通过种植骨髓溶液，脐带血，外周血，滑膜中的至少一种来培养间充质干细胞在装在容器中的液体培养基中的羊膜包含水作为主要成分，在37℃下比重为1.06〜1.10g / ml，培养温度为37±2℃ ℃，优选通过使用选自由聚乙烯吡咯烷酮涂覆的二氧化硅细粉末，蔗糖和表氯醇的水溶性共聚物和水溶性化合物中选择的至少一种来调节比重，所述水溶性化合物包括三碘芳环。

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