专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06372183B1 Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same 失效
标题翻译：使用其自动分析设备和检测细胞表面蛋白质和/或细胞质受体功能的测定方法
公开(公告)号：US06372183B1
公开(公告)日：2002-04-16
申请号：US08870537
申请日：1997-06-06
申请人： Michael Anthony Akong , Michael Miller Harpold , Gonul Velicelebi , Paul Brust
发明人： Michael Anthony Akong , Michael Miller Harpold , Gonul Velicelebi , Paul Brust
IPC分类号： G01N2164
CPC分类号： G01N33/6872 , G01N21/6428 , G01N21/6452 , G01N33/5008 , G01N33/502 , G01N33/5058 , G01N35/028 , G01N35/109 , G01N2035/042 , G01N2333/705 , G01N2500/00 , Y10S436/80 , Y10S436/807 , Y10S436/809 , Y10T436/11 , Y10T436/115831
摘要： Automated measurement apparatus and methods for automated drug screening procedures are provided. The apparatus is capable of initiating and measuring rapid or transient events, such as cell receptor and/or ion channel activity. In particular, the apparatus is capable of aligning with predetermined positions, one or more samples contained in a multi-well container, initiating the reaction with reagent addition and measuring a resultant attribute for a period of time. The apparatus can substantially continuously measure and record data corresponding to the measured attribute before, during and after initiation of the reaction so that a time course of the rapid or transient event may be determined. After the reaction(s) are complete in one or more wells of the multi-well container, the apparatus can align one or more different wells to be assayed with the predetermined position and repeat the cycle until a predetermined number of wells are assayed. Automated drug screening methods employing the apparatus are also provided for screening for compounds which activate, inhibit or potentiate cellular ion channel or receptor activity. The assays include fluorescent indicator-based assays that use viable cells containing effective levels of a fluorescent indicator which is responsive to changes in ion concentration. Activation of the ion channels or receptors is initiated in the automated measurement apparatus by injection into one or more predetermined cell-containing wells of a reagent which is a known or putative activator of the ion channels or receptors of interest. The resulting activity of the ion channels or receptors, which causes changes in ion concentration in the cytoplasm, is determined by measurement of fluorescence intensity changes of the indicator in response to an excitation wavelength.
摘要翻译：提供了自动化药物筛选程序的自动测量装置和方法。该装置能够启动和测量快速或瞬时事件，例如细胞受体和/或离子通道活性。特别地，该装置能够与预定位置对齐，一个或多个样品包含在多孔容器中，开始与试剂添加的反应并测量所得到的属性一段时间。该装置可以在反应开始之前，期间和之后基本连续地测量和记录对应于测量属性的数据，使得可以确定快速或瞬态事件的时间过程。在多孔容器的一个或多个孔中的反应完成之后，该装置可以将待测定的一个或多个不同孔与预定位置对准，并重复循环，直到测定预定数量的孔。还提供了使用该装置的自动药物筛选方法，用于筛选激活，抑制或增强细胞离子通道或受体活性的化合物。测定包括基于荧光指示剂的测定法，其使用含有有效水平的荧光指示剂的活细胞，其对离子浓度的变化有反应。离子通道或受体的激活在自动化测量装置中通过注射到一个或多个预定的含有细胞的孔中，该试剂是已知或推定的感兴趣的离子通道或受体的激活剂。通过测量响应于激发波长的指示剂的荧光强度变化来确定导致细胞质中离子浓度变化的离子通道或受体的所得活性。

2. 发明授权

US5981193A Human neuronal nicotinic acetylcholine receptor compositions and methods employing same 失效
标题翻译：人神经元烟碱乙酰胆碱受体组合物及其应用方法
公开(公告)号：US5981193A
公开(公告)日：1999-11-09
申请号：US938154
申请日：1992-11-30
申请人： Michael Miller Harpold , Steven Bradley Ellis , Paul Brust , Michael Akong , Gonul Velicelebi
发明人： Michael Miller Harpold , Steven Bradley Ellis , Paul Brust , Michael Akong , Gonul Velicelebi
IPC分类号： C07K14/705 , C12N15/12 , C12Q1/68 , C12N5/10
CPC分类号： C12Q1/6897 , C07K14/70571
摘要： Human neuronal nicotinic acetylcholine receptor subunits are described, as are methods for producing cells containing functional receptors employing such subunits. Also described are assay methods for determining the presence of functional HnAChRs in transfected cells, and for determining the agonist or antagonist activity of compounds with respect to such cells.
摘要翻译： PCT No.PCT / US91 / 02311 Sec。 371日期：1992年11月30日 102（e）日期1992年11月30日PCT 1991年4月3日PCT PCT。公开号WO91 / 15602 日期1991年10月17日描述人类神经元烟碱乙酰胆碱受体亚单位，以及使用这种亚单位产生含有功能受体的细胞的方法。还描述了用于确定转染细胞中功能性HnAChR的存在以及用于确定化合物相对于这种细胞的激动剂或拮抗剂活性的测定方法。

3. 发明授权

US5726035A Recombinant production of mammalian calcium channel gamma subunits 失效
标题翻译：哺乳动物钙通道γ亚单位的重组产生
公开(公告)号：US5726035A
公开(公告)日：1998-03-10
申请号：US336257
申请日：1994-11-07
申请人： Scott David Jay , Steven Bradley Ellis , Michael Miller Harpold , Kevin Peter Campbell
发明人： Scott David Jay , Steven Bradley Ellis , Michael Miller Harpold , Kevin Peter Campbell
IPC分类号： A61K38/00 , A61P43/00 , C07H21/02 , C07H21/04 , C07K14/00 , C07K14/705 , C07K19/00 , C12N5/10 , C12N15/09 , C12N15/12 , C12P21/02 , C12Q1/02 , C12Q1/68 , C12R1/91 , C07K14/435
CPC分类号： C07K14/705 , C12Q1/6876
摘要： Calcium channel .gamma.-subunit-encoding cDNAs, and related compositions and methods, are provided.
摘要翻译：提供钙通道γ-亚基编码cDNA，以及相关组合物和方法。

4. 发明授权

US5710250A Calcium channel Alpha 2 subunit polypeptides 失效
标题翻译：钙通道α2亚基多肽
公开(公告)号：US5710250A
公开(公告)日：1998-01-20
申请号：US435675
申请日：1995-05-05
申请人： Steven Bradley Ellis , Mark E. Williams , Michael Miller Harpold , Jean Sartor , Robert Brenner
发明人： Steven Bradley Ellis , Mark E. Williams , Michael Miller Harpold , Jean Sartor , Robert Brenner
IPC分类号： C07K14/705 , C12N15/12 , C12Q1/68 , G01N33/564 , G01N33/566 , G01N33/68 , C07K14/435 , C12N15/85
CPC分类号： G01N33/566 , C07K14/705 , C12Q1/6876 , C12Q1/6897 , G01N33/564 , G01N33/68 , G01N33/6893 , G01N2333/435 , G01N2333/705
摘要： .alpha..sub.1 - and .alpha..sub.2 - protein subunits of voltage-dependent calcium channels are provided.
摘要翻译：提供电压依赖性钙通道的α1-和α2-蛋白质亚基。

5. 发明授权

US5686241A Probes and assays for calcium channel .alpha..sub.2 subunit-encoding nucleic acids 失效
标题翻译：钙通道α2亚基编码核酸的探针和测定
公开(公告)号：US5686241A
公开(公告)日：1997-11-11
申请号：US314083
申请日：1994-09-28
申请人： Steven Bradley Ellis , Mark E. Williams , Michael Miller Harpold , Arnold Schwartz , Robert Brenner
发明人： Steven Bradley Ellis , Mark E. Williams , Michael Miller Harpold , Arnold Schwartz , Robert Brenner
IPC分类号： C07K14/705 , C12N15/12 , C12Q1/68 , G01N33/564 , G01N33/566 , G01N33/68
CPC分类号： G01N33/566 , C07K14/705 , C12Q1/6876 , C12Q1/6897 , G01N33/564 , G01N33/68 , G01N33/6893 , G01N2333/435 , G01N2333/705
摘要： Calcium channel (alpha).sub.1 -subunit and (alpha).sub.2 -subunit-encoding DNA, and related compositions and methods, are provided.
摘要翻译：提供了钙通道（α）1-亚基和（α）2-亚基编码DNA，以及相关组合物和方法。

6. 发明授权

US5670113A Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same 失效
标题翻译：使用其自动分析设备和检测细胞表面蛋白质和/或细胞质受体功能的测定方法
公开(公告)号：US5670113A
公开(公告)日：1997-09-23
申请号：US244985
申请日：1994-06-20
申请人： Michael Anthony Akong , Michael Miller Harpold , Gonul Velicelebi , Paul Brust
发明人： Michael Anthony Akong , Michael Miller Harpold , Gonul Velicelebi , Paul Brust
IPC分类号： G01N33/48 , G01N33/50 , G01N33/68 , G01N35/02 , G01N35/04 , G01N35/10 , G01N21/64 , G01J3/42
CPC分类号： G01N33/6872 , G01N21/6452 , G01N33/5008 , G01N33/502 , G01N33/5058 , G01N35/028 , G01N2035/042 , G01N21/6428 , G01N2333/705 , G01N2500/00 , G01N35/109 , Y10S436/80 , Y10S436/807 , Y10S436/809 , Y10T436/11 , Y10T436/115831
摘要： Automated measurement apparatus and methods for automated drug screening and for studying ion channel and cell surface receptor activity are provided. The apparatus is designed to initiate and measure rapid or transient events, such as cell receptor and/or ion channel activity. The apparatus can effect measurements of transient reactions in or more samples in a multi-well container, initiate the reaction with reagent addition and measure a resulting attribute of the sample for a period of time. The apparatus is capable of substantially continuously measuring and recording data corresponding to the measured attribute before, during and after initiation of the reaction so that a time course of the rapid or transient event is determined. Automated drug screening methods and methods for studying receptor and ion channel activity using the apparatus are also provided. The drug screening assays identify compounds that activate, inhibit or potentiate cellular ion channel or receptor activity.
摘要翻译： PCT No.PCT / US92 / 11090 Sec。 371日期：1994年6月20日 102（e）日期1994年6月20日PCT提交1992年12月13日PCT公布。出版物WO93 / 13423 日期1993年7月8日提供自动化药物筛选和研究离子通道和细胞表面受体活性的自动测量装置和方法。该装置被设计为启动和测量诸如细胞受体和/或离子通道活性的快速或瞬时事件。该装置可以实现多孔容器中或多个样品中的多个样品中的瞬态反应的测量，通过加入试剂启动反应并测量样品的结果属性一段时间。该装置能够在反应开始之前，期间和之后基本上连续地测量和记录对应于测量属性的数据，从而确定快速或瞬态事件的时间过程。还提供了使用该装置研究受体和离子通道活性的自动药物筛选方法和方法。药物筛选试验鉴定激活，抑制或增强细胞离子通道或受体活性的化合物。

7. 发明授权

US6127133A Automated analysis equipment and assay method for detecting cell surface protein function using same 失效
标题翻译：自动分析仪器和检测方法用于检测细胞表面蛋白质功能
公开(公告)号：US6127133A
公开(公告)日：2000-10-03
申请号：US229150
申请日：1994-04-18
申请人： Michael Anthony Akong , Michael Miller Harpold , Gonul Velicelebi , Paul Brust
发明人： Michael Anthony Akong , Michael Miller Harpold , Gonul Velicelebi , Paul Brust
IPC分类号： G01N33/48 , G01N33/50 , G01N33/68 , G01N35/02 , G01N35/04 , G01N35/10 , G01N33/53 , C12P21/06 , G01N15/06 , G01N33/566
CPC分类号： G01N33/6872 , G01N21/6452 , G01N33/5008 , G01N33/502 , G01N33/5058 , G01N35/028 , G01N2035/042 , G01N21/6428 , G01N2333/705 , G01N2500/00 , G01N35/109 , Y10S436/80 , Y10S436/807 , Y10S436/809 , Y10T436/11 , Y10T436/115831
摘要： Automated drug screening method and a method of studying receptor and ion-channel activity are provided.
摘要翻译：提供了自动药物筛选方法和研究受体和离子通道活性的方法。

8. 发明授权

US6057114A Automated assays and methods for detecting and modulating cell surface protein function 失效
标题翻译：用于检测和调节细胞表面蛋白质功能的自动测定和方法
公开(公告)号：US6057114A
公开(公告)日：2000-05-02
申请号：US434511
申请日：1995-05-04
申请人： Michael Anthony Akong , Michael Miller Harpold , Gonul Velicelebi , Paul Brust
发明人： Michael Anthony Akong , Michael Miller Harpold , Gonul Velicelebi , Paul Brust
IPC分类号： G01N33/48 , G01N33/50 , G01N33/68 , G01N35/02 , G01N35/04 , G01N35/10 , G01N33/533
CPC分类号： G01N33/6872 , G01N21/6452 , G01N33/5008 , G01N33/502 , G01N33/5058 , G01N35/028 , G01N2035/042 , G01N21/6428 , G01N2333/705 , G01N2500/00 , G01N35/109 , Y10S436/80 , Y10S436/807 , Y10S436/809 , Y10T436/11 , Y10T436/115831
摘要： Automated assays for detecting and measuring ion channel and cell surface receptor activity and for identifying compounds that modulate or potentiate such activity are provided. Among the assays are fluorescent indicator-based assays that use cells containing effective levels of a fluorescent indicator that is responsive to changes in ion concentration. Activation of the ion channels or receptors is initiated in the automated measurement apparatus by injection into one or more predetermined cell-containing wells of a reagent which is a known or possible activator or inhibitor of the ion channels or receptors of interest. The resulting activity of the ion channels or receptors, which causes changes in ion concentration in the cytoplasm, is determined by measurement of fluorescence intensity changes of the indicator in response to an excitation wavelength.
摘要翻译：提供了用于检测和测量离子通道和细胞表面受体活性以及鉴定调节或增强此类活性的化合物的自动化测定法。在这些测定中，基于荧光指示剂的测定法使用含有有效水平的对离子浓度变化作出反应的荧光指示剂的细胞。离子通道或受体的激活在自动测量装置中通过注射到已知或可能的活化剂或感兴趣的离子通道或受体的抑制剂的试剂的一个或多个预定的含细胞孔中开始。通过测量响应于激发波长的指示剂的荧光强度变化来确定导致细胞质中离子浓度变化的离子通道或受体的所得活性。

9. 发明授权

US6013474A Calcium channel compositions and methods 失效
标题翻译：钙通道组成和方法
公开(公告)号：US6013474A
公开(公告)日：2000-01-11
申请号：US884599
申请日：1997-06-27
申请人： Steven Bradley Ellis , Mark E. Williams , Michael Miller Harpold , Arnold Schwartz , Robert Brenner
发明人： Steven Bradley Ellis , Mark E. Williams , Michael Miller Harpold , Arnold Schwartz , Robert Brenner
IPC分类号： C12N1/19 , C07K14/00 , C07K14/47 , C07K14/705 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/12 , C12P21/00 , C12P21/02 , C12Q1/02 , C12Q1/68 , C12R1/645 , G01N33/564 , G01N33/68
CPC分类号： C07K14/705 , C12Q1/6876 , C12Q1/6897 , G01N33/5008 , G01N33/502 , G01N33/564 , G01N33/68 , G01N33/6872 , G01N33/6893 , C12Q2600/158
摘要： Calcium channel (alpha).sub.1 -subunit and (alpha).sub.2 -subunit-encoding DNA, and related compositions and methods, are provided.
摘要翻译：提供了钙通道（α）1-亚基和（α）2-亚基编码DNA，以及相关组合物和方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式