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1. 发明申请

US20100041070A1 Immunoassay and method of use 审中-公开
标题翻译：免疫测定和使用方法
公开(公告)号：US20100041070A1
公开(公告)日：2010-02-18
申请号：US12459903
申请日：2009-07-09
申请人： Murali D. Bandla , Matthew R. Chambers , Chester L. Sutula
发明人： Murali D. Bandla , Matthew R. Chambers , Chester L. Sutula
IPC分类号： G01N33/543
CPC分类号： G01N33/558 , G01N33/56911 , G01N2400/50
摘要： A method for performing an immunoassay is described. The method is particularly useful for detecting extracellular polysaccharide (EPS) and/or lipopolysaccharide (LPS) producing microorganisms. The method is particularly useful for detecting microorganisms which produce extracellular polysaccharides (EPS) also known as exocellular polysaccharides, capsule, and/or lipopolysaccharides (LPS). In a preferred method for detecting microorganisms which produce EPS, LPS, or both, the EPS and/or LPS is extracted from a sample with cetyltrimethylammonium bromide (CTAB) to produce molecular aggregates which are then preferentially bound to colored polystyrene latex particles over other components in the sample, and the bound EPS and/or LPS detected using a lateral flow immunoassay apparatus which has immobilized thereon antibodies specific for the EPS and/or LPS. The method can also be used to detect particular viruses, for example viruses of the potyviridae or tobamoviridae group.
摘要翻译：描述了进行免疫测定的方法。该方法特别适用于检测产生细胞外多糖（EPS）和/或脂多糖（LPS）的微生物。该方法特别可用于检测产生也被称为外细胞多糖，胶囊和/或脂多糖（LPS）的细胞外多糖（EPS）的微生物。在用于检测产生EPS，LPS或两者的微生物的优选方法中，用十六烷基三甲基溴化铵（CTAB）从样品中提取EPS和/或LPS以产生分子聚集体，然后优选与着色聚苯乙烯胶乳颗粒结合到其它组分上并且使用已经固定有EPS和/或LPS特异性抗体的侧流免疫测定装置检测结合的EPS和/或LPS。该方法还可以用于检测特定病毒，例如病毒科或鸟病毒科的病毒。

2. 发明授权

US07585641B2 Immunoassay and method of use 失效
标题翻译：免疫测定和使用方法
公开(公告)号：US07585641B2
公开(公告)日：2009-09-08
申请号：US10348078
申请日：2003-01-21
申请人： Murali D. Bandla , Matthew R. Chambers , Chester L. Sutula
发明人： Murali D. Bandla , Matthew R. Chambers , Chester L. Sutula
IPC分类号： C12N7/00 , C12N7/01 , C12N11/14 , C12N13/00 , C12Q1/06 , C12Q1/70 , G01N33/53
CPC分类号： G01N33/558 , G01N33/56911 , G01N2400/50
摘要： A method for performing an immunoassay is described. The method is particularly useful for detecting extracellular polysaccharide (EPS) and/or lipopolysaccharide (LPS) producing microorganisms. The method is particularly useful for detecting microorganisms which produce extracellular polysaccharides (EPS) also known as exocellular polysaccharides, capsule, and/or lipopolysaccharides (LPS). In a preferred method for detecting microorganisms which produce EPS, LPS, or both, the EPS and/or LPS is extracted from a sample with cetyltrimethylammonium bromide (CTAB) to produce molecular aggregates which are then preferentially bound to colored polystyrene latex particles over other components in the sample, and the bound EPS and/or LPS detected using a lateral flow immunoassay apparatus which has immobilized thereon antibodies specific for the EPS and/or LPS. The method can also be used to detect particular viruses, for example viruses of the potyviridae or tobamoviridae group.
摘要翻译：描述了进行免疫测定的方法。该方法特别适用于检测产生细胞外多糖（EPS）和/或脂多糖（LPS）的微生物。该方法特别可用于检测产生也被称为外细胞多糖，胶囊和/或脂多糖（LPS）的细胞外多糖（EPS）的微生物。在用于检测产生EPS，LPS或两者的微生物的优选方法中，用十六烷基三甲基溴化铵（CTAB）从样品中提取EPS和/或LPS以产生分子聚集体，然后优选与着色聚苯乙烯胶乳颗粒结合到其它组分上并且使用已经固定有EPS和/或LPS特异性抗体的侧流免疫测定装置检测结合的EPS和/或LPS。该方法还可以用于检测特定病毒，例如病毒科或鸟病毒科的病毒。

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