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1. 发明授权

US5232846A Method for producing a thermostable lipoprotein lipase from streptomyces 失效
标题翻译：从链霉菌制备热稳定性脂蛋白脂肪酶的方法
公开(公告)号：US5232846A
公开(公告)日：1993-08-03
申请号：US929744
申请日：1992-09-03
申请人： Akira Takeda , Tomoko Kamei , Masao Kageyama , Kenzo Motosugi
发明人： Akira Takeda , Tomoko Kamei , Masao Kageyama , Kenzo Motosugi
IPC分类号： C12N9/20 , C12Q1/44
CPC分类号： C12Q1/44 , C12N9/20 , Y10S435/906
摘要： The present invention is directed toward a thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, a process for producing the thermostable lipoprotein lipase, and a blood triglyceride determining reagent containing the thermostable lipoprotein lipase. The thermostable lipoprotein lipase exhibits about 100% retention of the hydrolyzing activity when treated in a buffer having a pH of from bout 4 to 7 at about 60.degree. C. for about 15 minutes and a glycerol forming activity/fatty acid forming activity ratio of at least about 15%. The process comprises cultivating a thermophilic actinomycetes, particularly Streptomyces 7825 (FERM P-9983, FERM BP-2489 is cultivated to produce a thermostable lipoprotein lipase.
摘要翻译：本发明涉及能够将脂蛋白中的甘油三酯水解成甘油和脂肪酸的热稳定性脂蛋白脂肪酶，产生热稳定性脂蛋白脂肪酶的方法和含有热稳定性脂蛋白脂肪酶的血液甘油三酯测定试剂。热稳定性脂蛋白脂肪酶在约60℃左右pH4〜7的缓冲液中处理约15分钟，甘油形成活性/脂肪酸形成活性比为至少约15％。该方法包括培养嗜热放线菌，特别是Streptomyces 7825（FERM P-9983，培养FERM BP-2489以产生热稳定性脂蛋白脂肪酶）。

2. 发明授权

US4812398A Reagent for measuring amylase activity and measuring method thereof 失效
标题翻译：测定淀粉酶活性的试剂及其测定方法
公开(公告)号：US4812398A
公开(公告)日：1989-03-14
申请号：US861306
申请日：1986-05-09
申请人： Hitoshi Kondo , Masao Kageyama
发明人： Hitoshi Kondo , Masao Kageyama
IPC分类号： C12Q1/40 , C12Q1/54
CPC分类号： C12Q1/40 , C12Q1/54
摘要： Disclosed is a reagent for measuring amylase activity in body fluids by cleaving an oligosaccharide having a defined chain length with amylase in body fluids to produce a glucose and measuring said glucose, characterized in that the reagent is divided to two portions, the first portion comprises an enzyme for converting a glucose and/or maltose naturally present in body fluids to glucose-6-phosphate, phosphoglucose isomerase, phosphofructokinase and adenosine-5'-triphosphate, and the second portion comprises said oligosaccharide being used as a substrate and a phosphoric acid ester of saccharides and/or saccharic acids; and a method for measuring amylase activity in body fluids comprising;eliminating glucose and/or maltose naturally present in body fluids with a first reagent comprising an enzyme for converting the glucose and/or maltose to glucose-6-phosphate, phosphoglucose isomerase, phosphofructokinase and adenosine-5'-triphosphate,adding a second reagent comprising a oligosaccharide substrate and a phosphoric acid ester of saccharides and/or saccharic acids to eliminate the phosphoglucose isomerase activity and to convert the oligosaccharide fragments formed by the action of amylase in body fluids to glucose by means of alpha-glucosidase or maltose phosphorylase, and measuring the amount of the obtained glucose.
摘要翻译：公开了一种用于通过用体液中的淀粉酶切割具有规定的链长的寡糖来测量体液中的淀粉酶活性以产生葡萄糖并测量所述葡萄糖的试剂，其特征在于，试剂被分成两部分，第一部分包含天然存在于体液中的葡萄糖和/或麦芽糖转化为葡萄糖-6-磷酸，磷酸葡萄糖异构酶，磷酸果糖激酶和腺苷-5'-三磷酸的酶，第二部分包含所述寡糖用作底物和磷酸酯的糖和/或糖酸; 和测定体液中的淀粉酶活性的方法，包括：用包含用于将葡萄糖和/或麦芽糖转化为葡萄糖-6-磷酸，磷酸葡萄糖异构酶，磷酸果糖激酶和腺苷-5'-三磷酸的酶的第一试剂消除体液中天然存在的葡萄糖和/或麦芽糖，加入第二试剂，寡糖底物和糖和/或糖酸的磷酸酯，以消除磷酸葡萄糖异构酶活性，并通过α-葡糖苷酶或麦芽糖磷酸化酶将体液中淀粉酶的作用形成的寡糖片段转化成葡萄糖，并测量所得葡萄糖的量。

3. 发明授权

US5244798A Reagent for determining triglycerides comprising a thermostable lipoprotein lipase from streptomyces 失效
标题翻译：用于测定含有来自链霉菌的热稳定性脂蛋白脂肪酶的甘油三酯的试剂
公开(公告)号：US5244798A
公开(公告)日：1993-09-14
申请号：US929743
申请日：1992-08-17
申请人： Akira Takeda , Tomoko Kamei , Masao Kageyama , Kenzo Motosugi
发明人： Akira Takeda , Tomoko Kamei , Masao Kageyama , Kenzo Motosugi
IPC分类号： C12N9/00 , C12N9/20 , C12Q1/34 , C12Q1/44 , C12R1/465 , G01N33/00
CPC分类号： C12Q1/44 , C12N9/20 , Y10S435/906
摘要： A reagent composition for determining blood triglycerides comprising a color former and a thermostable lipoprotein lipase obtained from Streptomyces 7825 (FERM P-9983, FERM BP-2489) is presented.
摘要翻译：提供了一种用于测定血甘油三酯的试剂组合物，其包含从Streptomyces 7825（FERM P-9983，FERM BP-2489）获得的成色剂和热稳定性脂蛋白脂肪酶。

4. 发明授权

US4331762A Bacillus stearothermophilus strain UK 788 and process for producing a useful enzyme 失效
标题翻译：嗜热脂肪芽孢杆菌菌株UK 788和生产有用酶的方法
公开(公告)号：US4331762A
公开(公告)日：1982-05-25
申请号：US209097
申请日：1980-11-21
申请人： Hiroshi Nakajima , Kazuhiko Nagata , Masao Kageyama , Toyohiko Suga , Tadao Suzuki , Kenzo Motosugi
发明人： Hiroshi Nakajima , Kazuhiko Nagata , Masao Kageyama , Toyohiko Suga , Tadao Suzuki , Kenzo Motosugi
IPC分类号： C12N9/00 , C12N9/04 , C12N9/12 , C12N1/20
CPC分类号： C12N9/0006 , C12N9/00 , C12N9/1258 , C12R1/07 , Y10S435/832
摘要： Strain UK 788 (FERM-P No. 5141) that belongs to Bacillus stearothermophilus, the cell of which is longer than about 10 microns and which permits easier release of intracellular components than a type culture Bacillus stearothermophilus, IAM 11001 is disclosed. Also, a process for producing a useful enzyme selected from the group consisting of a heat-resistant polynucleotide phosphorylase, heat-resistant maleate dehydrogenase, heat-resistant glucokinase, heat-resistant glucose-6-phosphate dehydrogenase and heat-resistant pyruvate kinase by culturing such UK 788 and recovering the desired enzyme from the culture is disclosed. Since the cell of the strain UK 788 is easy to settle and its membrane is also easy to break, the useful enzymes mentioned above can be efficiently produced on an industrial scale.
摘要翻译：属于嗜热脂肪芽孢杆菌的菌株UK788（FERM-P编号5141），其细胞长于约10微米，并且允许比类型培养的嗜热脂肪芽孢杆菌IAM 11001更容易释放细胞内组分。此外，通过培养生产选自耐热多核苷酸磷酸化酶，耐热马来酸脱氢酶，耐热葡萄糖激酶，耐热葡萄糖-6-磷酸脱氢酶和耐热丙酮酸激酶的有用酶的方法公开了这样的英国788并从培养物中回收所需的酶。由于菌株UK 788的细胞容易沉降并且其膜也易于破裂，所以上述有用的酶可以在工业规模上有效地生产。

5. 发明授权

US5173417A Thermostable lipoprotein lipase, process for producing the same, and triglyceride determining reagent using the same 失效
标题翻译：可热稳定的脂蛋白脂肪酶，其制备方法和使用其的TRIGLYCERIDE测定试剂
公开(公告)号：US5173417A
公开(公告)日：1992-12-22
申请号：US391017
申请日：1989-08-09
申请人： Akira Takeda , Tomoko Kamei , Masao Kageyama , Kenzo Motosugi
发明人： Akira Takeda , Tomoko Kamei , Masao Kageyama , Kenzo Motosugi
IPC分类号： C12N9/00 , C12N9/20 , C12Q1/34 , C12Q1/44 , C12R1/465 , G01N33/00
CPC分类号： C12Q1/44 , C12N9/20 , Y10S435/906
摘要： The present invention is directed toward a thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, a process for producing the thermostable lipoprotein lipase, and a blood triglyceride determining reagent containing the thermostable lipoprotein lipase. The thermostable lipoprotein lipase exhibits about 100% retention of the hydrolyzing activity when treated in a buffer having a pH of from about 4 to 7 at about 60.degree. C. for about 15 minutes and a glycerol forming activity/fatty acid forming activity ratio of at least about 15%. The process comprises cultivating a thermophilic actinomycetes, particularly Streptomyces 7825 (FERM P-9983, FERM BP-2489 and recovering lipoprotein lipase from the culture.

6. 发明授权

US4794084A Acyl-CoA synthetase 失效
标题翻译：酰基辅酶A合成酶
公开(公告)号：US4794084A
公开(公告)日：1988-12-27
申请号：US11478
申请日：1987-02-05
申请人： Mitsuo Watanabe , Hiromi Sato , Tomoko Kamei , Masao Kageyama
发明人： Mitsuo Watanabe , Hiromi Sato , Tomoko Kamei , Masao Kageyama
IPC分类号： C12N9/00 , C12N1/20
CPC分类号： C12N9/93 , C12R1/38 , Y10S435/874
摘要： An acyl-CoA synthetase is disclosed having the following characteristics:(a) Reaction:Capable of acting on saturated or unsaturated lower to higher fatty acids to produce a CoA derivative thereof (Acyl-CoA), adenosine monophosphate and pyrophosporic acid, in the presence of adenosine triphosphate and coenzyme A;(b) Stability:Having a residual activity of not less than about 50% after being incubated in a buffer (pH about 7.5 for about 15 minutes at a temperature of about 55.degree. C.; and(c) Optimum reaction temperature:Having an optimum reaction temperature in the range of from 50.degree. to 60.degree. C.Also disclosed is Psuedomonas UKSW-3733 strain which is capable of producing the acyl-CoA synthetase, which has an optimum temperature for growth in the range of from 45.degree. to 55.degree. C. and is incapable of assimilating carbonates.The acyl-CoA synthetase is highly stable and, therefore, can be used for preparation of clinical diagnosis kits capable of giving accurate test results over an extended period of time.
摘要翻译：公开了具有以下特征的酰基-CoA合成酶：（a）反应：能够在饱和或不饱和的低级至高级脂肪酸上作用，以产生其CoA衍生物（Acyl-CoA），单磷酸腺苷和焦磷酸，的三磷酸腺苷和辅酶A; （b）稳定性：在缓冲液（pH约7.5左右，在约55℃的温度下约15分钟后）具有不小于约50％的残余活性;和（c）最佳反应温度：具有 50℃至60℃范围内的最佳反应温度。还公开了能够产生酰基辅酶A合成酶的Psuedomonas UKSW-3733菌株，其具有在45℃至55℃范围内生长的最佳温度并且不能同化碳酸酯，酰基辅酶A合成酶是高度稳定的，因此可用于制备能够在更长时间内提供准确测试结果的临床诊断试剂盒。

7. 发明授权

US5610045A Producing a high content of acetate kinase using bacillus stearothermophilus 失效
标题翻译：使用嗜热脂肪芽孢杆菌产生高含量的醋酸激酶
公开(公告)号：US5610045A
公开(公告)日：1997-03-11
申请号：US458770
申请日：1995-06-02
申请人： Masao Kageyama , Toyohiko Suga , Kenzo Motosugi , Hiroshi Nakajima
发明人： Masao Kageyama , Toyohiko Suga , Kenzo Motosugi , Hiroshi Nakajima
IPC分类号： C12N1/20 , C12N9/12 , C21N9/12 , C21N1/20
CPC分类号： C12N1/20 , C12N9/12
摘要： A process is described for cultivating thermophilic bacteria of the genus Bacillus having the ability to produce acetate kinase, wherein said cultivation is carried out continuously at a dilution rate (D) in the range from about 0.9 .mu..sub.max to 1.0 .mu..sub.max, wherein D is the dilution rate (1/hr), and .mu..sub.max is the maximum specific growth rate (1/hr), to thereby produce bacterial cells having a high content of acetate kinase.
摘要翻译：描述了用于培养具有产生乙酸激酶能力的芽孢杆菌属嗜热细菌的方法，其中所述培养以约0.9至最大1.0m的稀释速率（D）连续进行，其中D为稀释率（1 / hr）和mu max是最大比生长速率（1 / hr），从而产生具有高含量醋酸激酶的细菌细胞。

8. 发明授权

US4740458A Reagent for assaying creatine kinase 失效
标题翻译：用于测定肌酸激酶的试剂
公开(公告)号：US4740458A
公开(公告)日：1988-04-26
申请号：US784432
申请日：1985-10-04
申请人： Hitoshi Kondo , Masao Kageyama , Kosuke Tomita
发明人： Hitoshi Kondo , Masao Kageyama , Kosuke Tomita
IPC分类号： C12N9/12 , C12Q1/32 , C12Q1/48 , C12Q1/50 , C12Q1/54 , C12Q1/26
CPC分类号： C12Q1/50 , C12N9/12 , Y10S435/81
摘要： A reagent system for assaying creatine kinase is disclosed. The reagent system consisting essentially of a first reagent comprising glucose-6-phosphate dehydrogenase, .beta.-nicotinamideadenine dinucleotide (phosphate), and adenosine diphosphate, and a second reagent comprising creatine phosphate, said second reagent being maintained at a pH of from 7.5 to 10, and at least one of said first reagent and said second reagent containing glucokinase and glucose. The creatine kinase-assaying reagent exhibits remarkably improved stability in a dissolved state so that it can be prepared in large quantities and can be conventionally utilized to cope with urgent clinical examinations.
摘要翻译：公开了一种用于测定肌酸激酶的试剂系统。基本上由包含葡萄糖-6-磷酸脱氢酶，β-烟酰胺腺嘌呤二核苷酸（磷酸酯）和二磷酸腺苷的第一试剂和包含磷酸肌酸的第二试剂组成的试剂系统，所述第二试剂保持在7.5至10的pH ，并且所述第一试剂和所述第二试剂中的至少一种含有葡糖激酶和葡萄糖。肌酸激酶测定试剂在溶解状态下显示出显着提高的稳定性，因此其可以大量制备，并且可以常规地用于处理紧急临床检查。

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