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    • 2. 发明授权
    • Method of preparing angiotensin converting enzyme inhibitors
    • 制备血管紧张素转换酶抑制剂的方法
    • US5243027A
    • 1993-09-07
    • US737852
    • 1991-07-31
    • Tsutomu MimuraYasuhiro KohamaKazuhiko NagataRyoichi Tsurutani
    • Tsutomu MimuraYasuhiro KohamaKazuhiko NagataRyoichi Tsurutani
    • A61K38/00C12N9/04
    • C12N9/0006A61K38/00
    • A method of preparing an angiotensin converting enzyme inhibitor having the amino acid sequence selected from the group consisting of:Gly-Lys-Glu-Ile-Ile-Val-Lys-Ala-Glu-Arg (SEQ ID No: 1),Gly-Lys-Met-Val-Lys-Val-Val-Ser-Trp-Tyr (SEQ ID No: 2) andAla-Tyr-Ile-Ala-Ser-Lys-Gly-Leu (SEQ ID No: 3); which comprises the steps of:(a) disrupting bacteria to obtain a cell extract, wherein the disruption is at least one of mechanical and chemical disruption;(b) exposing said cell extract to an acid mixture comprising acetic acid and hydrochloric acid, wherein said acid mixture comprises acetic acid in a concentration of 0.01 to 10M and hydrochloric acid in a concentration of 1 to 100 mM, to produce an acid hydrolysate; and(c) purifying from said acid hydrolysate a peptide having the amino acid sequence selected from the group consisting of:Gly-Lys-Glu-Ile-Ile-Val-Lys-Ala-Glu-Arg (SEQ ID No: 1),Gly-Lys-Met-Val-Lys-Val-Val-Ser-Trp-Tyr (SEQ ID No: 2) andAla-Tyr-Ile-Ala-Ser-Lys-Gly-Leu (SEQ ID No: 3).
    • 一种制备具有选自Gly-Lys-Glu-Ile-Ile-Val-Lys-Ala-Glu-Arg(SEQ ID No:1),Gly- Lys-Met-Val-Lys-Val-Val-Ser-Trp-Tyr(SEQ ID No:2)和Ala-Tyr-Ile-Ala-Ser-Lys-Gly-Leu(SEQ ID No:3); 其包括以下步骤:(a)破坏细菌以获得细胞提取物,其中所述破坏是机械和化学破坏中的至少一种; (b)将所述细胞提取物暴露于含有乙酸和盐酸的酸混合物中,其中所述酸混合物包含浓度为0.01至10M的乙酸和浓度为1至100mM的盐酸,以产生酸水解产物; 和(c)从所述酸水解产物中纯化具有选自Gly-Lys-Glu-Ile-Ile-Val-Lys-Ala-Glu-Arg(SEQ ID No:1)的氨基酸序列的肽, Gly-Lys-Met-Val-Lys-Val-Val-Ser-Trp-Tyr(SEQ ID No:2)和Ala-Tyr-Ile-Ala-Ser-Lys-Gly-Leu(SEQ ID No:3)。
    • 5. 发明授权
    • Reagent for measurement of magnesium ion
    • 用于测量镁离子的试剂
    • US5683889A
    • 1997-11-04
    • US960364
    • 1993-09-23
    • Hitoshi KondouKazuhiko NagataKazue Kawahara
    • Hitoshi KondouKazuhiko NagataKazue Kawahara
    • C12Q1/48G01N33/84C12Q1/54C12Q1/00
    • G01N33/84C12Q1/48
    • The present invention relates to a reagent for measurement of magnesium ions utilizing a complex of magnesium ion with adenosine 5'-triphosphate as a substrate of glucokinase derived from bacteria of the genus Zymomonas. By using this reagent, enough measuring range to measure a magnesium ion in various samples such as biological fluids, foodstuffs, etc. can be accomplished and, at the same time, the measurement can be made with high precision and reliability even in a sample containing a magnesium ion in low concentration. Further, the reagent of the present invention is superior in operation property by reducing a dilution operation, and has sufficient performance for a routine method for determination of magnesium ions.
    • PCT No.PCT / JP92 / 00278 Sec。 371日期:1993年9月23日 102(e)日期1993年9月23日PCT 1991年3月9日PCT公布。 第WO92 / 16650号公报 日期:1992年10月1日本发明涉及利用镁离子与腺苷5'-三磷酸复合物作为源自发酵菌属细菌的葡萄糖激酶的底物的镁离子测定试剂。 通过使用该试剂,可以实现足够的测量范围来测量各种样品如生物液体,食品等中的镁离子,并且同时可以高精度和可靠地进行测量,即使在含有 低浓度的镁离子。 此外,本发明的试剂通过减少稀释操作而具有优异的操作性能,并且对于用于测定镁离子的常规方法具有足够的性能。