专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US10081838B2 Gene expression signature for IL-6/STAT3 signaling pathway and use thereof 有权
公开(公告)号：US10081838B2
公开(公告)日：2018-09-25
申请号：US14398532
申请日：2013-05-03
申请人： Zhong Wu , Martin Schlumpberger , John Dicarlo , Vikram Devgan , Yexun Wang
发明人： Zhong Wu , Martin Schlumpberger , John Dicarlo , Vikram Devgan , Yexun Wang
IPC分类号： C12Q1/6876 , C12Q1/6883
CPC分类号： C12Q1/6876 , C12N15/113 , C12N2310/14 , C12Q1/6883 , C12Q1/6886 , C12Q2600/158 , C12Q2600/16
摘要： The present invention relates to a set of biomarkers, microarrays that provide for detection thereof, an expression signature comprising 16 genes or a subset thereof, and the use thereof in determining the regulation status of IL-6/STAT3 signaling pathway in a cell sample or subject, as well as compositions for the detection thereof. The regulation status of IL-6/STAT3 signaling pathway in a cell sample or subject may be assayed based on the level of expression of one or more of these genes. The methods and compositions provided herein may be used to evaluate IL-6/STAT3 pathway regulation status in a sample; classify a cell sample as having a deregulated or regulated IL-6/STAT3 signaling pathway; determine whether an agent modulates the IL-6/STAT3 signaling pathway; predict the response of a subject to an agent that modulates the IL-6/STAT3 signaling pathway; assign treatment to a subject; and/or evaluate the pharmacodynamic effects of therapies designed to regulate IL-6/STAT3 pathway signaling. Expression of the biomarkers is preferably determined by RT-PCR using SYBR Green methods, and the expression data analyzed and compared to a control sample by use of the random forest method.

2. 发明申请

US20090202998A1 METHOD FOR THE EXTRACTION OF BIOMOLECULES FROM FIXED TISSUES 审中-公开
标题翻译：从固定组织中提取生物分子的方法
公开(公告)号：US20090202998A1
公开(公告)日：2009-08-13
申请号：US12097488
申请日：2006-12-18
申请人： Martin Schlumpberger , Ralf Peist
发明人： Martin Schlumpberger , Ralf Peist
IPC分类号： A61K31/7088 , C02F3/34 , C12Q1/68
CPC分类号： C12N15/1003
摘要： The present invention relates to a method for treating a fixed biological sample, comprising the steps of the method: i) provision of a fixed biological sample, ii) contacting the fixed biological sample with an aqueous solution comprising at least one nucleophilic reagent, and iii) heating the biological sample contacted with the aqueous solution. The invention also relates to the biological sample obtainable by this method, to the use of a nucleophilic reagent for the treatment of a fixed biological sample, to a kit for isolating a biomolecule from a fixed biological sample, to the use of this kit, and to a method for the treatment of a disease.
摘要翻译：本发明涉及一种治疗固定生物样品的方法，包括以下步骤：i）提供固定生物样品，ii）使固定生物样品与包含至少一种亲核试剂的水溶液接触，iii ）加热与水溶液接触的生物样品。本发明还涉及通过该方法可获得的生物样品，将亲核试剂用于固定的生物样品的处理，将生物分子从固定生物样品分离的试剂盒用于该试剂盒的使用，以及涉及治疗疾病的方法。

3. 发明申请

US20150232926A1 GENE EXPRESSION SIGNATURE FOR IL-6/STAT3 SIGNALING PATHWAY AND USE THEREOF 审中-公开
标题翻译： IL-6 / STAT3信号途径的基因表达及其用途
公开(公告)号：US20150232926A1
公开(公告)日：2015-08-20
申请号：US14398532
申请日：2013-05-03
申请人： Zhong Wu , Martin Schlumpberger , John Dicarlo , Vikram Devgan , Yexun Wang
发明人： Zhong Wu , Martin Schlumpberger , John Dicarlo , Vikram Devgan , Yexun Wang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12N15/113 , C12N2310/14 , C12Q1/6886 , C12Q2600/158 , C12Q2600/16
摘要： The present invention relates to a set of biomarkers, microarrays that provide for detection thereof, an expression signature comprising 16 genes or a subset thereof, and the use thereof in determining the regulation status of IL-6/STAT3 signaling pathway in a cell sample or subject, as well as compositions for the detection thereof. The regulation status of IL-6/STAT3 signaling pathway in a cell sample or subject may be assayed based on the level of expression of one or more of these genes. The methods and compositions provided herein may be used to evaluate IL-6/STAT3 pathway regulation status in a sample; classify a cell sample as having a deregulated or regulated IL-6/STAT3 signaling pathway; determine whether an agent modulates the IL-6/STAT3 signaling pathway; predict the response of a subject to an agent that modulates the IL-6/STAT3 signaling pathway; assign treatment to a subject; and/or evaluate the pharmacodynamic effects of therapies designed to regulate IL-6/STAT3 pathway signaling. Expression of the biomarkers is preferably determined by RT-PCR using SYBR Green methods, and the expression data analyzed and compared to a control sample by use of the random forest method.
摘要翻译：本发明涉及提供其检测的一组生物标志物，微阵列，包含16个基因或其子集的表达标签，以及其用于确定细胞样品中IL-6 / STAT3信号传导途径的调节状态或受试者以及用于检测的组合物。可以基于这些基因中的一种或多种的表达水平来测定细胞样品或受试者中IL-6 / STAT3信号通路的调节状态。本文提供的方法和组合物可用于评估样品中的IL-6 / STAT3通路调节状态; 将细胞样品分类为具有去调节或调节的IL-6 / STAT3信号传导途径; 确定试剂是否调节IL-6 / STAT3信号通路; 预测受试者对调节IL-6 / STAT3信号通路的药剂的反应; 为受试者分配治疗; 和/或评估旨在调节IL-6 / STAT3通路信号传导的疗法的药效学作用。优选通过使用SYBR Green方法的RT-PCR来确定生物标志物的表达，并且通过使用随机森林法分析表达数据并与对照样品进行比较。

4. 发明授权

US11542494B2 Method for isolating a target nucleic acid including small target nucleic acids with high yield 有权
公开(公告)号：US11542494B2
公开(公告)日：2023-01-03
申请号：US13820228
申请日：2011-09-02
申请人： Vera Holländer , Gabriele Christoffel , Martin Schlumpberger
发明人： Vera Holländer , Gabriele Christoffel , Martin Schlumpberger
IPC分类号： C12Q1/68 , C12N15/10 , C07H21/04
摘要： The present invention pertains to a method for isolating a target nucleic acid including small target nucleic acids from a sample, said method comprising at least the following steps a) binding at least a portion of the target nucleic acid including small target nucleic acids to a nucleic acid binding solid phase comprised in a column by passing the sample through said column, b) performing an enzymatic and/or chemical treatment on the nucleic acid binding solid phase while the target nucleic acid is bound to said solid phase, c) collecting at least a portion of the small target nucleic acids released from the solid phase during said treatment of step b) as flow-through, d) contacting said flow-through which comprises small target nucleic acids mixed with a recovery solution with a nucleic acid binding solid phase for binding the contained small target nucleic acids to said nucleic acid binding solid phase, e) optionally performing an elution. The present invention results in a considerable increase in the yield of small target nucleic acids in the isolated target nucleic acid because it allows to efficiently capture and recover small target nucleic acids.

5. 发明授权

US09097628B2 Method and kit for processing wax-embedded biological samples 有权
标题翻译：蜡嵌入生物样品的加工方法和试剂盒
公开(公告)号：US09097628B2
公开(公告)日：2015-08-04
申请号：US13996437
申请日：2011-12-23
申请人： Martin Schlumpberger
发明人： Martin Schlumpberger
IPC分类号： G01N1/30 , C12N15/10
CPC分类号： G01N1/30 , C12N15/1003 , C12N15/1006 , C12N15/101
摘要： The present invention relates to a method for processing a wax-embedded biological sample, the use of poly(organosiloxane)s for liquefying the embedding medium of a wax-embedded biological sample and a kit for processing a wax-embedded biological sample.
摘要翻译：本发明涉及一种用于处理嵌蜡生物样品的方法，使用聚（有机硅氧烷）来液化嵌入蜡的生物样品的包埋介质和用于处理蜡嵌入的生物样品的试剂盒。

6. 发明申请

US20130164825A1 METHOD FOR ISOLATING A TARGET NUCLEIC ACID INCLUDING SMALL TARGET NUCLEIC ACIDS WITH HIGH YIELD 审中-公开
标题翻译：用于分离靶向核酸的方法，包括具有高YIELD的小目标核酸
公开(公告)号：US20130164825A1
公开(公告)日：2013-06-27
申请号：US13820228
申请日：2011-09-02
申请人： Vera Holländer , Gabriele Christoffel , Martin Schlumpberger
发明人： Vera Holländer , Gabriele Christoffel , Martin Schlumpberger
IPC分类号： C07H1/08
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention pertains to a method for isolating a target nucleic acid including small target nucleic acids from a sample, said method comprising at least the following steps a) binding at least a portion of the target nucleic acid including small target nucleic acids to a nucleic acid binding solid phase comprised in a column by passing the sample through said column, b) performing an enzymatic and/or chemical treatment on the nucleic acid binding solid phase while the target nucleic acid is bound to said solid phase, c) collecting at least a portion of the small target nucleic acids released from the solid phase during said treatment of step b) as flow-through, d) contacting said flow-through which comprises small target nucleic acids mixed with a recovery solution with a nucleic acid binding solid phase for binding the contained small target nucleic acids to said nucleic acid binding solid phase, e) optionally performing an elution. The present invention results in a considerable increase in the yield of small target nucleic acids in the isolated target nucleic acid because it allows to efficiently capture and recover small target nucleic acids.
摘要翻译：本发明涉及从样品中分离包含小靶核酸的靶核酸的方法，所述方法至少包括以下步骤：a）将包含小靶核酸的靶核酸的至少一部分结合至核酸通过使样品通过所述柱而包含在柱中的酸结合固相，b）对核酸结合固相进行酶和/或化学处理，同时靶核酸与所述固相结合，c）至少收集在步骤b）的所述处理期间从固相中释放的一部分小目标核酸作为流通，d）使所述流通接触，所述流过包含与回收溶液混合的小目标核酸与核酸结合固相用于将所含的小靶核酸与所述核酸结合固相结合，e）任选地进行洗脱。本发明导致分离的靶核酸中小靶核酸的产量显着增加，因为其允许有效捕获和回收小靶核酸。

7. 发明申请

US20130280728A1 METHOD AND KIT FOR PROCESSING WAX-EMBEDDED BIOLOGICAL SAMPLES 有权
标题翻译：用于处理嵌入式生物样品的方法和工具包
公开(公告)号：US20130280728A1
公开(公告)日：2013-10-24
申请号：US13996437
申请日：2011-12-23
申请人： Martin Schlumpberger
发明人： Martin Schlumpberger
IPC分类号： G01N1/30
CPC分类号： G01N1/30 , C12N15/1003 , C12N15/1006 , C12N15/101
摘要： The present invention relates to a method for processing a wax-embedded biological sample, the use of poly(organosiloxane)s for liquefying the embedding medium of a wax-embedded biological sample and a kit for processing a wax-embedded biological sample.
摘要翻译：本发明涉及一种用于处理嵌蜡生物样品的方法，使用聚（有机硅氧烷）来液化嵌入蜡的生物样品的包埋介质和用于处理蜡嵌入的生物样品的试剂盒。

8. 发明授权

US08563262B2 Permanent inactivation of nucleases 有权
标题翻译：核酸酶永久失活
公开(公告)号：US08563262B2
公开(公告)日：2013-10-22
申请号：US12680515
申请日：2008-09-26
申请人： Gabriele Christoffel , Martin Schlumpberger , Heike Glowatz
发明人： Gabriele Christoffel , Martin Schlumpberger , Heike Glowatz
IPC分类号： C12Q1/44
CPC分类号： C12N15/1003
摘要： The present invention relates to the use of reagent for permanently inactivating nucleases wherein the reagent comprises an oxidizing agent, a protein denaturant and optionally a pH adjustor and to a method for permanently inactivating nucleases using said reagent.
摘要翻译：本发明涉及用于永久灭活核酸酶的试剂的用途，其中试剂包含氧化剂，蛋白质变性剂和任选的pH调节剂，以及使用所述试剂永久灭活核酸酶的方法。

9. 发明申请

US20130231460A1 METHOD AND DEVICE FOR CONCENTRATING TARGET COMPOUNDS 审中-公开
标题翻译：用于浓缩目标化合物的方法和装置
公开(公告)号：US20130231460A1
公开(公告)日：2013-09-05
申请号：US13821509
申请日：2011-09-07
申请人： Christoph Erbacher , Vera Holländer , Markus Müller , Martin Schlumpberger , Marion Schmidt , Markus Sprenger-Haussels
发明人： Christoph Erbacher , Vera Holländer , Markus Müller , Martin Schlumpberger , Marion Schmidt , Markus Sprenger-Haussels
IPC分类号： G01N1/40 , C12Q1/68
CPC分类号： G01N1/405 , B01L3/5023 , B01L3/5029 , B01L2300/043 , B01L2300/0609 , C12Q1/6806
摘要： The present invention relates to a method for concentrating one or more target compounds in a liquid sample, a device for carrying out this method and a kit for processing a biological sample comprising such a device.
摘要翻译：本发明涉及浓缩液体样品中一种或多种目标化合物的方法，用于实施该方法的装置和用于处理包含这种装置的生物样品的试剂盒。

10. 发明申请

US20100209993A1 PERMANENT INACTIVATION OF NUCLEASES 有权
标题翻译：核子的永久灭活
公开(公告)号：US20100209993A1
公开(公告)日：2010-08-19
申请号：US12680515
申请日：2008-09-26
申请人： Gabriele Christoffel , Martin Schlumpberger , Heike Glowatz
发明人： Gabriele Christoffel , Martin Schlumpberger , Heike Glowatz
IPC分类号： C12N9/99
CPC分类号： C12N15/1003
摘要： The present invention relates to the use of reagent for permanently inactivating nucleases wherein the reagent comprises an oxidizing agent, a protein denaturant and optionally a pH adjustor and to a method for permanently inactivating nucleases using said reagent.
摘要翻译：本发明涉及用于永久灭活核酸酶的试剂的用途，其中试剂包含氧化剂，蛋白质变性剂和任选的pH调节剂，以及使用所述试剂永久灭活核酸酶的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式