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1. 发明授权

US08273688B2 Collection of HCDR3 regions and uses therefor 有权
标题翻译：收集HCDR3地区及其用途
公开(公告)号：US08273688B2
公开(公告)日：2012-09-25
申请号：US12158181
申请日：2006-12-15
申请人： Markus Enzelberger , Stefanie Thiel , Josef Prassler , Stefanie Urlinger , Christine Rothe
发明人： Markus Enzelberger , Stefanie Thiel , Josef Prassler , Stefanie Urlinger , Christine Rothe
IPC分类号： C40B40/08 , C40B40/10 , C40B50/00
CPC分类号： C07K16/00 , C07K2317/565
摘要： The present invention is directed to the preparation and use of a collection of antibody heavy chain complementarity determining region 3 (HCDR3) members, where diversity of the collection is a function of the length of the HCDR3 members. The diversity of the collection of HCDR3 regions substantially represents the natural amino acid distribution of HCDR3 in the human repertoire. This natural amino acid distribution can be represented by biasing the complete random distribution of amino acids, accordingly, in the HCDR3 encoding DNA sequence by using trinucleotide mutagenesis (TRIM) technology. A collection of HCDR3 members of the invention each can be comprised within a variable region of an antibody (or fragment thereof) to form a library of synthetic antibodies or antibody fragments. The invention also provides nucleic acid molecules encoding such diverse collection and methods of making and using the same.
摘要翻译：本发明涉及抗体重链互补决定区3（HCDR3）成员的集合的制备和使用，其中集合的多样性是HCDR3成员的长度的函数。 HCDR3区域的多样性基本上代表HCDR3在人体内的天然氨基酸分布。通过使用三核苷酸诱变（TRIM）技术，通过偏置氨基酸的完全随机分布，因此在编码DNA序列的HCDR3中可以表示天然氨基酸分布。本发明的HCDR3成员的集合各自可以包含在抗体（或其片段）的可变区内，以形成合成抗体或抗体片段的文库。本发明还提供了编码这种不同收集物的核酸分子及其制备和使用方法。

2. 发明授权

US09267952B2 Deconvolution method 有权
标题翻译：去卷积法
公开(公告)号：US09267952B2
公开(公告)日：2016-02-23
申请号：US13124127
申请日：2009-11-04
申请人： Markus Enzelberger
发明人： Markus Enzelberger
IPC分类号： C40B30/04 , G01N33/68
CPC分类号： G01N33/6845 , C40B30/04
摘要： The present invention relates generally to novel applications in combating infectious disease, cancer, allergy and autoimmune diseases. In one aspect, the invention relates to identifying one or more protein binding moieties of interest. In another aspect, the present invention relates to identifying one or more candidate vaccines.
摘要翻译：本发明一般涉及抗感染性疾病，癌症，变态反应和自身免疫性疾病的新型应用。一方面，本发明涉及鉴定感兴趣的一种或多种蛋白质结合部分。另一方面，本发明涉及鉴定一种或多种候选疫苗。

3. 发明授权

US08550119B2 Microfabricated elastomeric valve and pump systems 有权
公开(公告)号：US08550119B2
公开(公告)日：2013-10-08
申请号：US11932552
申请日：2007-10-31
申请人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake , Markus Enzelberger , Mark L. Adams , Carl L. Hansen
发明人： Marc A. Unger , Hou-Pu Chou , Todd A. Thorsen , Axel Scherer , Stephen R. Quake , Markus Enzelberger , Mark L. Adams , Carl L. Hansen
IPC分类号： F15C3/00 , F16K31/126
CPC分类号： F15C3/00 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/0481 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , F04B19/006 , F04B43/043 , F16K99/0001 , F16K99/0026 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , Y10T137/2202 , Y10T137/2224 , Y10T137/87885
摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.

4. 发明申请

US20120077713A1 Collection and Methods for its Use 有权
标题翻译：收集和使用方法
公开(公告)号：US20120077713A1
公开(公告)日：2012-03-29
申请号：US13321564
申请日：2010-05-29
申请人： Markus Enzelberger , Stefanie Urlnger , Thomas Tiller , Josef Prassler , Tanja Herrmann
发明人： Markus Enzelberger , Stefanie Urlnger , Thomas Tiller , Josef Prassler , Tanja Herrmann
IPC分类号： C40B40/08 , C12N15/63 , C12N1/21 , C07K16/00 , C12N5/10 , C40B50/06 , C12N15/57 , C40B40/10 , C12N1/19
CPC分类号： C07K16/005 , C07K16/00 , C07K16/32 , C07K2317/21 , C07K2317/56 , C07K2317/565 , C07K2317/567 , C12N15/1093 , C40B30/04 , C40B40/08 , C40B50/06
摘要： The present disclosure enables methods of identifying the VH and VL class pairs in the human immune repertoire, determining the VH and VL class pairs that are most prevalent and those having favorable biophysical properties. More specifically, the collections of the present disclosure comprise the most prevalent and/or preferred VH and VL class pairings with highly diversified CDRs.
摘要翻译：本公开使得能够鉴定人免疫谱中的VH和VL类对的方法，确定最普遍的VH和VL类对以及具有有利的生物物理性质的那些。更具体地，本公开的集合包含具有高度多样化的CDR的最普遍和/或优选的VH和VL类配对。

5. 发明申请

US20100317546A1 Display Vectors and Methods and Uses Thereof 审中-公开
标题翻译：显示向量及其方法和用途
公开(公告)号：US20100317546A1
公开(公告)日：2010-12-16
申请号：US12812236
申请日：2009-01-09
申请人： Markus Enzelberger , Philippe Thiel , Solveig Peters , Rüdiger Neun , Stephen Yoder
发明人： Markus Enzelberger , Philippe Thiel , Solveig Peters , Rüdiger Neun , Stephen Yoder
IPC分类号： C40B40/02 , C12N15/79 , C12N15/85 , C12N5/10 , C12N5/24 , C12Q1/68 , C12P21/00
CPC分类号： C12N15/1037
摘要： The present invention relates, in one aspect, to a vector comprising (a) a first polynucleotide capable of encoding a first (poly)peptide comprising at least one cysteine residue, and (b) a second polynucleotide capable of encoding a second (poly)peptide which is a cell surface anchor comprising at least one cysteine residue, wherein the vector is operable in a eukaryotic host cell to express and to cause or allow the attachment of said first (poly)peptide to said second (poly)peptide by formation of a disulfide bond between said cysteine residues comprised within said first (polypeptide and said second (poly)peptide, respectively, wherein said first (poly)peptide is exhibited at the surface of a eukaryotic host cell.
摘要翻译：本发明在一个方面涉及包含（a）能够编码包含至少一个半胱氨酸残基的第一（多）肽的第一多核苷酸的载体，和（b）能够编码第二（多聚）肽，其是包含至少一个半胱氨酸残基的细胞表面锚，其中所述载体可在真核宿主细胞中操作以表达并引起或允许所述第一（多）肽通过形成所述第二（多）肽而形成分别包含在所述第一（多肽和所述第二（多））肽内的所述半胱氨酸残基之间的二硫键，其中所述第一（多）肽显示在真核宿主细胞的表面。

6. 发明申请

US20090088346A1 NOVEL COLLECTION OF HCDR3 REGIONS AND USES THEREFOR 有权
标题翻译：新的收集HCDR3地区及其用途
公开(公告)号：US20090088346A1
公开(公告)日：2009-04-02
申请号：US12158181
申请日：2006-12-15
申请人： Markus Enzelberger , Stefanie Thiel
发明人： Markus Enzelberger , Stefanie Thiel
IPC分类号： C40B40/08 , C40B40/10 , C40B50/00
CPC分类号： C07K16/00 , C07K2317/565
摘要： The present invention is directed to the preparation and use of a collection of antibody heavy chain complementarity determining region 3 (HCDR3) members, where diversity of the collection is a function of the length of the HCDR3 members. The diversity of the collection of HCDR3 regions substantially represents the natural amino acid distribution of HCDR3 in the human repertoire. This natural amino acid distribution can be represented by biasing the complete random distribution of amino acids, accordingly, in the HCDR3 encoding DNA sequence by using trinucleotide mutagenesis (TRIM) technology. A collection of HCDR3 members of the invention each can be comprised within a variable region of an antibody (or fragment thereof) to form a library of synthetic antibodies or antibody fragments. The invention also provides nucleic acid molecules encoding such diverse collection and methods of making and using the same.
摘要翻译：本发明涉及抗体重链互补决定区3（HCDR3）成员的集合的制备和使用，其中集合的多样性是HCDR3成员的长度的函数。 HCDR3区域的多样性基本上代表HCDR3在人体内的天然氨基酸分布。通过使用三核苷酸诱变（TRIM）技术，通过偏置氨基酸的完全随机分布，因此在编码DNA序列的HCDR3中可以表示天然氨基酸分布。本发明的HCDR3成员的集合各自可以包含在抗体（或其片段）的可变区内，以形成合成抗体或抗体片段的文库。本发明还提供了编码这种不同收集物的核酸分子及其制备和使用方法。

7. 发明授权

US07216671B2 Microfabricated elastomeric valve and pump systems 有权
标题翻译：微型弹性阀和泵系统
公开(公告)号：US07216671B2
公开(公告)日：2007-05-15
申请号：US11056451
申请日：2005-02-10
申请人： Marc Unger , Hou-Pu Chou , Todd Thorsen , Axel Scherer , Stephen Quake , Markus Enzelberger , Mark Adams , Carl Hansen
发明人： Marc Unger , Hou-Pu Chou , Todd Thorsen , Axel Scherer , Stephen Quake , Markus Enzelberger , Mark Adams , Carl Hansen
IPC分类号： F15C1/06
CPC分类号： B05D3/04 , B01J2219/00355 , B01J2219/00378 , B01J2219/00396 , B01J2219/00398 , B01J2219/00439 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/54 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0605 , B01L2200/10 , B01L2300/0681 , B01L2300/0861 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2300/18 , B01L2400/046 , B01L2400/0481 , B01L2400/06 , B01L2400/0655 , B01L2400/0688 , B32B2037/1081 , B65B31/00 , B81B2201/036 , B81B2201/054 , B81C1/00119 , B81C2201/019 , C12Q1/6832 , C12Q1/6874 , C30B29/54 , F04B43/043 , F15C1/06 , F15C3/00 , F15C5/00 , F16K99/0001 , F16K99/0015 , F16K99/0046 , F16K99/0051 , F16K99/0059 , F16K2099/0074 , F16K2099/0076 , F16K2099/0078 , F16K2099/008 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T137/0379 , Y10T137/0424 , Y10T137/206 , Y10T137/2082 , Y10T137/2164 , Y10T137/2169 , Y10T137/2174 , Y10T137/2202 , Y10T137/2224 , Y10T137/3084 , Y10T137/7837 , Y10T137/86027 , Y10T156/10 , Y10T428/24479 , Y10T428/24612 , C12Q2535/125
摘要： A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
摘要翻译：一种制造弹性体结构的方法，包括：在第一微加工模具的顶部上形成第一弹性体层，所述第一微加工模具具有形成沿所述第一弹性体层的底表面延伸的第一凹槽的第一凸起突起; 在第二微加工模具的顶部上形成第二弹性体层，所述第二微加工模具具有第二凸起突起，所述第二凸起突起形成沿所述第二弹性体层的底表面延伸的第二凹槽; 将第二弹性体层的底表面粘合到第一弹性体层的顶表面上，使得控制通道在第一和第二弹性体层之间的第二凹部中形成; 以及将第一弹性体层定位在平面基底的顶部上，使得流动通道在第一弹性体层和平面基底之间的第一凹部中形成。

8. 发明申请

US20140011698A1 SIMULTANEOUS DETECTION OF BIOMOLECULES IN SINGLE CELLS 审中-公开
标题翻译：同时检测单细胞中的生物分子
公开(公告)号：US20140011698A1
公开(公告)日：2014-01-09
申请号：US13989814
申请日：2011-11-30
申请人： Markus Enzelberger , Andreas Boll , Beate Diefenbach-Streiber , Guenter Roth , Felix von Stetten , Fabian Stumpf
发明人： Markus Enzelberger , Andreas Boll , Beate Diefenbach-Streiber , Guenter Roth , Felix von Stetten , Fabian Stumpf
IPC分类号： G01N33/53
CPC分类号： G01N33/5308 , C12Q1/6834 , C12Q1/6869 , C12Q2523/303 , C12Q2527/109 , C12Q2527/119 , C12Q2531/113 , C12Q2563/149 , C12Q2563/159 , G01N33/543 , G01N33/6842
摘要： The present invention provides methods, immunoassays, kits and devices pertaining to the detection of multiple biomolecules from single cells or other biological entities. It also enables the highly parallel detection of interacting biomolecules from such entities.
摘要翻译：本发明提供了与从单细胞或其他生物实体检测多种生物分子有关的方法，免疫测定，试剂盒和装置。它还能够高度平行地检测来自这些实体的相互作用的生物分子。

9. 发明授权

US08551733B2 Production of oligoclonal mixtures of immunoglobulins in single cells 失效
标题翻译：在单细胞中生产免疫球蛋白的寡克隆混合物
公开(公告)号：US08551733B2
公开(公告)日：2013-10-08
申请号：US13147383
申请日：2010-02-08
申请人： Markus Enzelberger
发明人： Markus Enzelberger
IPC分类号： C12P21/06
CPC分类号： C07K16/00 , A61K2039/505
摘要： The present invention provides methods to produce mixtures of exogenous proteins in single cells. Preferably said exogenous proteins are heteromeric and multimeric proteins, such as immunoglobulins. The method enables the controlled expression of proteins, thereby allowing the correct formation and assembly of multimeric proteins, such as immunoglobulins.
摘要翻译：本发明提供在单细胞中产生外源蛋白质的混合物的方法。优选地，所述外源蛋白质是异聚和多聚体蛋白质，例如免疫球蛋白。该方法能够控制蛋白质的表达，从而允许多聚体蛋白质如免疫球蛋白的正确形成和装配。

10. 发明申请

US20120028302A1 Production of oligoclonal mixtures of immunoglobulins in single cells 失效
标题翻译：在单细胞中生产免疫球蛋白的寡克隆混合物
公开(公告)号：US20120028302A1
公开(公告)日：2012-02-02
申请号：US13147383
申请日：2010-02-08
申请人： Markus Enzelberger
发明人： Markus Enzelberger
IPC分类号： C12P21/00
CPC分类号： C07K16/00 , A61K2039/505
摘要： The present invention provides methods to produce mixtures of exogenous proteins in single cells. Preferably said exogenous proteins are heteromeric and multimeric proteins, such as immunoglobulins. The method enables the controlled expression of proteins, thereby allowing the correct formation and assembly of multimeric proteins, such as immunoglobulins.
摘要翻译：本发明提供在单细胞中产生外源蛋白质的混合物的方法。优选地，所述外源蛋白质是异聚和多聚体蛋白质，例如免疫球蛋白。该方法能够控制蛋白质的表达，从而允许多聚体蛋白质如免疫球蛋白的正确形成和装配。

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