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    • 3. 发明授权
    • Loader and loader control system
    • US07881845B2
    • 2011-02-01
    • US11959722
    • 2007-12-19
    • Mark Nichols
    • Mark Nichols
    • E02F9/26G01C21/00G06F19/00
    • E02F3/3414E02F3/431E02F9/264
    • A loader and a control system for a loader provide for monitoring the position of a part of the implement carried by the loader. The loader has a body with left and right upright tower portions, and a loader drive system including ground engaging drive elements. Left and right interconnected lift arm assemblies each have an implement lift arm pivotally connected with a corresponding tower portion of the body at a lift arm pivot point. A lift actuator is connected between the body and the lift arm. The implement is pivotally connected with the lift arm assemblies about an implement pivot axis. The lift arm pivot point and the implement pivot axis in side elevation define a straight reference line. At least one implement tilt actuator is connected between at least one of the lift arm assemblies and the implement. A position sensor is mounted on the body at the level of, or above, the lift arm pivot points. An inclinometer is movable with the left and right interconnected lift arm assemblies to provide an indication of the inclination of the lift arm assemblies along the straight reference line. An angle sensor provides an indication of the orientation of said implement with respect to said left and right interconnected lift arm assemblies. The control is responsive to the position sensor, the inclinometer, and the angle sensor. The control determines the position of the position sensor and the position of a part of the implement with respect to the position sensor.
    • 5. 发明授权
    • Small interfering RNA libraries and methods of synthesis and use
    • 小干扰RNA文库和合成和使用方法
    • US07524653B2
    • 2009-04-28
    • US10842353
    • 2004-05-10
    • Mark NicholsRichard Steinman
    • Mark NicholsRichard Steinman
    • C12N15/64C12N15/00C07H21/04
    • C12N15/1138C12N15/1093C12N15/111C12N2310/111C12N2310/14C12N2310/53C12N2330/31
    • In one aspect, the invention provides a random or semirandom siRNA (encoding) library. Another aspect of the invention pertains to methods for construction of random or semirandom siRNA (encoding) libraries. Another aspect of the invention is vector systems for use in constructing siRNA libraries and/or that can express single siRNAs and siRNA libraries both constitutively and in an inducible fashion. In another aspect, the invention provides a method of using an siRNA library. The siRNA library is introduced into a population of cells. The population of cells then is subjected to a selection process to select a subpopulation of cells exhibiting a different behavioral, biochemical, chemical, functional, molecular, morphological, phenotypic, or physical property from the remainder of population. Following the selection process, the subpopulation of cells can be isolated, analyzed, and/or cloned as desired. Such analysis of the subpopulation can be identification and sequencing of the siRNA species responsible for the different properties of the subpopulation relative to the remainder of the population. Alternatively, the subpopulation can be further analyzed by genomic, proteomic, and/or cellomic assays. Where such genomic, proteomic, and/or cellomic assays are employed, the method can produce several useful bioinformatics products. Specific siRNAs identified through this process may have direct therapeutic value.
    • 一方面,本发明提供了随机或半免疫siRNA(编码)文库。 本发明的另一方面涉及用于构建随机或半随机siRNA(编码)文库的方法。 本发明的另一方面是用于构建siRNA文库的载体系统和/或可组成型和诱导型表达单个siRNA和siRNA文库。 另一方面,本发明提供了使用siRNA文库的方法。 将siRNA文库导入细胞群。 然后对细胞群体进行选择过程,从其余群体中选择表现出不同行为,生物化学,化学,功能,分子,形态,表型或物理性质的细胞亚群。 在选择过程之后,可以根据需要分离,分析和/或克隆细胞亚群。 亚群的这种分析可以是负责亚群相对于其余群体的不同性质的siRNA物种的鉴定和测序。 或者,亚群可以通过基因组,蛋白质组学和/或纤维素测定进一步分析。 在使用这种基因组,蛋白质组学和/或纤维素测定法的情况下,该方法可以产生几种有用的生物信息学产物。 通过该方法鉴定的特异性siRNA可具有直接的治疗价值。
    • 8. 发明申请
    • Small interfering RNA libraries and methods of synthesis and use
    • 小干扰RNA文库和合成和使用方法
    • US20070166720A1
    • 2007-07-19
    • US10842353
    • 2004-05-10
    • Mark NicholsRichard Steinman
    • Mark NicholsRichard Steinman
    • C40B30/06C40B40/08
    • C12N15/1138C12N15/1093C12N15/111C12N2310/111C12N2310/14C12N2310/53C12N2330/31
    • In one aspect, the invention provides a random or semirandom siRNA (encoding) library. Another aspect of the invention pertains to methods for construction of random or semirandom siRNA (encoding) libraries. Another aspect of the invention is vector systems for use in constructing siRNA libraries and/or that can express single siRNAs and siRNA libraries both constitutively and in an inducible fashion. In another aspect, the invention provides a method of using an siRNA library. The siRNA library is introduced into a population of cells. The population of cells then is subjected to a selection process to select a subpopulation of cells exhibiting a different behavioral, biochemical, chemical, functional, molecular, morphological, phenotypic, or physical property from the remainder of population. Following the selection process, the subpopulation of cells can be isolated, analyzed, and/or cloned as desired. Such analysis of the subpopulation can be identification and sequencing of the siRNA species responsible for the different properties of the subpopulation relative to the remainder of the population. Alternatively, the subpopulation can be further analyzed by genomic, proteomic, and/or cellomic assays. Where such genomic, proteomic, and/or cellomic assays are employed, the method can produce several useful bioinformatics products. Specific siRNAs identified through this process may have direct therapeutic value.
    • 一方面,本发明提供了随机或半免疫siRNA(编码)文库。 本发明的另一方面涉及用于构建随机或半随机siRNA(编码)文库的方法。 本发明的另一方面是用于构建siRNA文库的载体系统和/或可组成型和诱导型表达单个siRNA和siRNA文库。 另一方面,本发明提供了使用siRNA文库的方法。 将siRNA文库导入细胞群。 然后对细胞群体进行选择过程,从其余群体中选择表现出不同行为,生物化学,化学,功能,分子,形态,表型或物理性质的细胞亚群。 在选择过程之后,可以根据需要分离,分析和/或克隆细胞亚群。 亚群的这种分析可以是负责亚群相对于其余群体的不同性质的siRNA物种的鉴定和测序。 或者,亚群可以通过基因组,蛋白质组学和/或纤维素测定进一步分析。 在使用这种基因组,蛋白质组学和/或纤维素测定法的情况下,该方法可以产生几种有用的生物信息学产物。 通过该方法鉴定的特异性siRNA可具有直接的治疗价值。
    • 10. 发明申请
    • Method for augmenting radio positioning system using single fan laser
    • 使用单扇激光器增强无线电定位系统的方法
    • US20070058161A1
    • 2007-03-15
    • US11214221
    • 2005-08-27
    • Mark NicholsNicholas TalbotGary Cain
    • Mark NicholsNicholas TalbotGary Cain
    • G01C1/00G01B11/26
    • G01S19/04G01S19/07G01S19/10G01S19/44G01S19/46
    • A method of augmenting a mobile radio positioning system (Mobile_RADPS) by using a stationary fan laser transmitter. A rover comprises the mobile radio positioning system (Mobile_RADPS) integrated with a mobile laser detector. The stationary fan laser transmitter is integrated with a stationary radio positioning system (Stationary_RADPS). The method comprises the following steps: (A)generating a single sloping fan beam by the stationary fan laser transmitter; (B) detecting the single sloping fan beam generated by the stationary fan laser transmitter by using the mobile laser detector; and (C) timing the fan laser beam strike at the rover's location and using the timing of the fan laser beam strike at the rover's location to improve an accuracy in determination of position coordinates of the rover.
    • 一种通过使用固定式风扇激光发射器来增强移动无线电定位系统(Mobile_RADPS)的方法。 流动站包括与移动激光检测器集成的移动无线电定位系统(Mobile_RADPS)。 固定式风扇激光发射机与固定无线电定位系统(Stationary_RADPS)集成。 该方法包括以下步骤:(A)由固定式风扇激光发射器产生单个倾斜扇形光束; (B)使用移动式激光检测器检测由固定式风扇激光发射器产生的单斜扇形光束; 并且(C)使风扇激光束在流动站的位置处定时,并且使用风扇激光束在流动站的位置处的打击时间来提高确定流动站的位置坐标的准确度。