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1. 发明授权

US06916661B2 Microparticles with multiple fluorescent signals and methods of using same 有权
公开(公告)号：US06916661B2
公开(公告)日：2005-07-12
申请号：US10694084
申请日：2003-10-28
申请人： Mark B. Chandler , Don J. Chandler
发明人： Mark B. Chandler , Don J. Chandler
IPC分类号： C12Q1/68 , G01N15/14 , G01N33/00 , G01N33/58 , G01N33/48 , G01N33/536
CPC分类号： G01N33/587 , C12Q1/6816 , G01N15/14 , G01N33/582 , Y10S435/81 , Y10S435/975 , Y10S436/805 , Y10S436/808 , Y10S436/823 , Y10S977/707 , Y10S977/773 , Y10S977/904 , Y10S977/92 , Y10T436/10 , Y10T436/109163 , Y10T436/13 , C12Q2563/155 , C12Q2563/107 , C12Q2537/143 , C12Q2565/626
摘要： This invention provides a novel fluorescent particle including a core or carrier particle having on its surface a plurality of smaller polymeric particles or nanoparticles, which are stained with different fluorescent dyes. When excited by a light source they are capable of giving off multiple fluorescent emissions simultaneously, which is useful for multiplexed analysis of a plurality of analytes in a sample. The coupled complex particles carrying on their surface fluorescent nanoparticles, methods of preparing such polymer particles, and various applications and methods of using such particles are claimed.

2. 发明授权

US06268222B1 Microparticles attached to nanoparticles labeled with flourescent dye 有权
标题翻译：附着在用荧光染料标记的纳米颗粒上的微粒
公开(公告)号：US06268222B1
公开(公告)日：2001-07-31
申请号：US09234841
申请日：1999-01-22
申请人： Mark B. Chandler , Don J. Chandler
发明人： Mark B. Chandler , Don J. Chandler
IPC分类号： G01N33536
CPC分类号： G01N33/54346 , G01N15/14 , G01N33/582 , G01N33/587 , G01N2015/1018
摘要： This invention provides a novel fluorescent article comprising a core or carrier particle having on its surface a plurality of smaller polymeric particles or nanoparticles, which are stained with different fluorescent dyes. When excited by a light source they are capable of giving off multiple fluorescent emissions simultaneously, which is useful for multiplexed analysis of a plurality of analytes in a sample. The coupled complex particles carrying on their surface fluorescent nanoparticles, methods of preparing such polymer articles, and various applications and methods of using such particles are claimed.
摘要翻译：本发明提供了一种新颖的荧光制品，其包含在其表面上具有多个较小的聚合物颗粒或纳米颗粒的芯或载体颗粒，其被不同的荧光染料染色。当被光源激发时，它们能够同时发出多个荧光发射，这对于样品中多个分析物的多重分析是有用的。涉及其表面上携带有荧光纳米颗粒的偶联复合颗粒，制备这种聚合物制品的方法以及使用这种颗粒的各种应用和方法。

3. 发明申请

US20090215077A1 METHODS FOR DIAGNOSIS OF ACUTE CORONARY SYNDROME 审中-公开
标题翻译：急性冠状动脉综合征诊断方法
公开(公告)号：US20090215077A1
公开(公告)日：2009-08-27
申请号：US12417496
申请日：2009-04-02
申请人： Mark B. CHANDLER , Michael D. SPAIN , James MAPES , George RODGERS
发明人： Mark B. CHANDLER , Michael D. SPAIN , James MAPES , George RODGERS
IPC分类号： G01N33/53 , C12Q1/00 , C12Q1/48
CPC分类号： C12Q1/48 , C12Q1/37 , G01N33/573 , G01N33/6893 , G01N2333/91188 , G01N2333/96486 , G01N2800/324
摘要： Provided are methods for the detection and diagnosis of acute coronary syndrome or ACS. The methods are based on the discovery that abnormal levels of selected analytes in sample fluid, typically blood samples, of patients who are at risk are supportive of a diagnosis of ACS. At least two new biomarkers for ACS are thus disclosed, MMP-3 and SGOT. Altogether the concentrations of twelve analytes provide a sensitive and selective picture of the patient's condition, namely, whether the patient is suffering a heart attack. Other important biomarkers for ACS are described, including but not limited to IL-18, Factor VII, ICAM-1, Creatine Kinase-MB, MCP-1, Myoglobin, C Reactive Protein, von Willebrand Factor, TIMP-1, Ferritin, Glutathione S-Transferase, Prostate Specific Antigen (free), IL-3, Tissue Factor, alpha-Fetoprotein, Prostatic Acid Phosphatase, Stem Cell Factor, MIP-1-beta, Carcinoembryonic Antigen, IL-13, TNF-alpha, IgE, Fatty Acid Binding Protein, ENA-78, IL-1-beta, Brain-Derived Nerotrophic Factor, Apolipoprotein A1, Serum Amyloid P, Growth Hormone, Beta-2 microglobulin, Lipoprotein (a), MMP-9, Thyroid Stimulating hormone, alpha-2 Macroglobulin, Complement 3, IL-7, Leptin, and IL-6. Kits containing reagents to assist in the analysis of fluid samples are also described.
摘要翻译：提供了用于急性冠状动脉综合征或ACS的检测和诊断的方法。这些方法基于以下发现：患有风险的患者的样品液体（通常为血液样本）中所选分析物的异常水平支持ACS的诊断。因此公开了至少两种用于ACS的新生物标志物，MMP-3和SGOT。总共有十二种分析物的浓度可以提供对病人状况的敏感和有选择性的图片，即患者是否患有心脏病发作。描述了ACS的其他重要生物标志物，包括但不限于IL-18，因子VII，ICAM-1，肌酸激酶-MB，MCP-1，肌红蛋白，C反应蛋白，血管性血友病因子，TIMP-1，铁蛋白，谷胱甘肽 S转移酶，前列腺特异性抗原（游离），IL-3，组织因子，α-甲胎蛋白，前列腺酸性磷酸酶，干细胞因子，MIP-1-β，癌胚抗原，IL-13，TNF-α，IgE，脂肪酸结合蛋白，ENA-78，IL-1-β，脑源性神经营养因子，载脂蛋白A1，血清淀粉样蛋白P，生长激素，β-2微球蛋白，脂蛋白（a），MMP-9，甲状腺刺激激素， 2大球蛋白，补体3，IL-7，瘦蛋白和IL-6。还描述了含有辅助分析流体样品的试剂的试剂盒。

4. 发明授权

US06524793B1 Multiplexed analysis of clinical specimens apparatus and method 失效
标题翻译：临床标本装置和方法的多重分析
公开(公告)号：US06524793B1
公开(公告)日：2003-02-25
申请号：US09335719
申请日：1999-06-18
申请人： Van S. Chandler , R. Jerrold Fulton , Mark B. Chandler
发明人： Van S. Chandler , R. Jerrold Fulton , Mark B. Chandler
IPC分类号： G01N3353
CPC分类号： G01N33/54313 , G01N15/1012 , G01N15/1456 , G01N33/5094 , G01N2015/1018 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2015/149 , Y10S435/973
摘要： A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. The inventive technology enables the simultaneous, and automated, detection and interpretation of multiple biomolecules or DNA sequences in real-time while also reducing the cost of performing diagnostic and genetic assays.
摘要翻译：用于酶，DNA片段，抗体和其他生物分子的多重诊断和遗传分析的方法包括以下步骤：构建适当标记的珠粒，将珠粒暴露于临床样品，并通过流式细胞术分析组合的样品/珠粒。流式细胞仪测量用于实时分类暴露珠粒内的珠子，并根据在实时分析中获得的累积数据，为用户生成文字说明。本发明的技术使得能够实时地同时和自动地检测和解释多个生物分子或DNA序列，同时还降低了进行诊断和遗传测定的成本。

5. 发明授权

US07141431B2 Microparticles with multiple fluorescent signals and methods of using same 有权
公开(公告)号：US07141431B2
公开(公告)日：2006-11-28
申请号：US11173331
申请日：2005-06-30
申请人： Mark B. Chandler , Don J. Chandler , Jason Bedre
发明人： Mark B. Chandler , Don J. Chandler , Jason Bedre
IPC分类号： G01N33/48 , G01N33/536
CPC分类号： G01N33/587 , C12Q1/6816 , G01N15/14 , G01N33/582 , Y10S435/81 , Y10S435/975 , Y10S436/805 , Y10S436/808 , Y10S436/823 , Y10S977/707 , Y10S977/773 , Y10S977/904 , Y10S977/92 , Y10T436/10 , Y10T436/109163 , Y10T436/13 , C12Q2563/155 , C12Q2563/107 , C12Q2537/143 , C12Q2565/626
摘要： This invention provides a novel fluorescent particle including a core or carrier particle having on its surface a plurality of smaller polymeric particles or nanoparticles, which are stained with different fluorescent dyes. When excited by a light source they are capable of giving off multiple fluorescent emissions simultaneously, which is useful for multiplexed analysis of a plurality of analytes in a sample. The coupled complex particles carrying on their surface fluorescent nanoparticles, methods of preparing such polymer particles, and various applications and methods of using such particles are claimed.

6. 发明授权

US06939720B2 Multiplexed analysis of clinical specimens apparatus and method 失效
标题翻译：临床标本装置和方法的多重分析
公开(公告)号：US06939720B2
公开(公告)日：2005-09-06
申请号：US10329391
申请日：2002-12-27
申请人： Van S. Chandler , R. Jerrold Fulton , Mark B. Chandler
发明人： Van S. Chandler , R. Jerrold Fulton , Mark B. Chandler
IPC分类号： G01N15/10 , G01N15/14 , G01N33/50 , G01N33/543 , G01N33/553
CPC分类号： G01N33/54313 , G01N15/1012 , G01N15/1456 , G01N33/5094 , G01N2015/1018 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2015/149 , Y10S435/973
摘要： A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. The inventive technology enables the simultaneous, and automated, detection and interpretation of multiple biomolecules or DNA sequences in real-time while also reducing the cost of performing diagnostic and genetic assays.
摘要翻译：用于酶，DNA片段，抗体和其他生物分子的多重诊断和遗传分析的方法包括以下步骤：构建适当标记的珠粒，将珠粒暴露于临床样品，并通过流式细胞术分析组合的样品/珠粒。流式细胞仪测量用于实时分类暴露珠粒内的珠子，并根据在实时分析中获得的累积数据，为用户生成文字说明。本发明的技术使得能够实时地同时和自动地检测和解释多个生物分子或DNA序列，同时还降低了进行诊断和遗传测定的成本。

7. 发明授权

US5981180A Multiplexed analysis of clinical specimens apparatus and methods 失效
标题翻译：临床标本装置和方法的多重分析
公开(公告)号：US5981180A
公开(公告)日：1999-11-09
申请号：US540814
申请日：1995-10-11
申请人： Van S. Chandler , R. Jerrold Fulton , Mark B. Chandler
发明人： Van S. Chandler , R. Jerrold Fulton , Mark B. Chandler
IPC分类号： G01N15/10 , G01N15/14 , G01N33/50 , G01N33/543 , C12Q1/68
CPC分类号： G01N33/54313 , G01N15/1012 , G01N15/1456 , G01N33/5094 , B01J2219/00459 , B01J2219/00576 , G01N2015/1018 , G01N2015/1477 , G01N2015/1486 , G01N2015/1488 , G01N2015/149 , Y10S435/973
摘要： A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. The inventive technology enables the simultaneous, and automated, detection and interpretation of multiple biomolecules or DNA sequences in real-time while also reducing the cost of performing diagnostic and genetic assays.
摘要翻译：用于酶，DNA片段，抗体和其他生物分子的多重诊断和遗传分析的方法包括以下步骤：构建适当标记的珠粒，将珠粒暴露于临床样品，并通过流式细胞术分析组合的样品/珠粒。流式细胞仪测量用于实时分类暴露珠粒内的珠子，并根据在实时分析中获得的累积数据，为用户生成文字说明。本发明的技术使得能够实时地同时和自动地检测和解释多个生物分子或DNA序列，同时还降低了进行诊断和遗传测定的成本。

8. 发明授权

US06649414B1 Microparticles with multiple fluorescent signals and methods of using same 有权
标题翻译：具有多个荧光信号的微粒及其使用方法
公开(公告)号：US06649414B1
公开(公告)日：2003-11-18
申请号：US09639818
申请日：2000-08-17
申请人： Mark B. Chandler , Don J. Chandler
发明人： Mark B. Chandler , Don J. Chandler
IPC分类号： G01N3348
CPC分类号： G01N33/587 , C12Q1/6816 , G01N15/14 , G01N33/582 , Y10S435/81 , Y10S435/975 , Y10S436/805 , Y10S436/808 , Y10S436/823 , Y10S977/707 , Y10S977/773 , Y10S977/904 , Y10S977/92 , Y10T436/10 , Y10T436/109163 , Y10T436/13 , C12Q2563/155 , C12Q2563/107 , C12Q2537/143 , C12Q2565/626
摘要： This invention provides a novel fluorescent particle including a core or carrier particle having on its surface a plurality of smaller polymeric particles or nanoparticles, which are stained with different fluorescent dyes. When excited by a light source they are capable of giving off multiple fluorescent emissions simultaneously, which is useful for multiplexed analysis of a plurality of analytes in a sample. The coupled complex particles carrying on their surface fluorescent nanoparticles, methods of preparing such polymer particles, and various applications and methods of using such particles are claimed.
摘要翻译：本发明提供了一种新颖的荧光颗粒，其包括在其表面上具有多个较小的聚合物颗粒或纳米颗粒的芯或载体颗粒，其被不同的荧光染料染色。当被光源激发时，它们能够同时发出多个荧光发射，这对于样品中多个分析物的多重分析是有用的。涉及其表面上携带有荧光纳米颗粒的偶联复合颗粒，制备这种聚合物颗粒的方法以及使用这种颗粒的各种用途和方法。

9. 发明授权

US06449562B1 Multiplexed analysis of clinical specimens apparatus and method 有权
标题翻译：临床标本装置与方法的多重分析
公开(公告)号：US06449562B1
公开(公告)日：2002-09-10
申请号：US09000286
申请日：1998-08-18
申请人： Van S. Chandler , Jerrold R. Fulton , Mark B. Chandler
发明人： Van S. Chandler , Jerrold R. Fulton , Mark B. Chandler
IPC分类号： G01N3348
CPC分类号： G01N33/537 , C12Q1/00 , G01N33/582 , G06F19/20 , G06F19/24 , G06F19/28 , Y10S707/99931
摘要： A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry is disclosed. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. The inventive technology enables the simultaneous, and automated, detection and interpretation of multiple biomolecules or DNA sequences in real-time while also reducing the cost of performing diagnostic and genetic assays.
摘要翻译：一种用于酶，DNA片段，抗体和其他生物分子的多重诊断和遗传分析的方法包括以下步骤：构建适当标记的珠粒，将珠粒暴露于临床样品，并通过流式细胞术分析组合的样品/珠粒。流式细胞仪测量用于实时分类暴露珠粒内的珠子，并根据在实时分析中获得的累积数据，为用户生成文字说明。本发明的技术使得能够实时地同时和自动地检测和解释多个生物分子或DNA序列，同时还降低了进行诊断和遗传测定的成本。

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