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1. 发明授权

US09492528B2 Influenza virus-like particles (VLPS) comprising hemagglutinin 有权
标题翻译：包含血凝素的流感病毒样颗粒（VLPS）
公开(公告)号：US09492528B2
公开(公告)日：2016-11-15
申请号：US13003570
申请日：2009-07-02
申请人： Marc-Andre D'Aoust , Manon Couture , Frédéric Ors , Sonia Trepanier , Pierre-Olivier Lavoie , Michèle Dargis , Louis-Philippe Vezina , Nathalie Landry
发明人： Marc-Andre D'Aoust , Manon Couture , Frédéric Ors , Sonia Trepanier , Pierre-Olivier Lavoie , Michèle Dargis , Louis-Philippe Vezina , Nathalie Landry
IPC分类号： A61K39/145 , C12N15/82 , C12N7/04 , C12N15/44 , C07K14/005 , C12N7/00 , A61K39/12 , A61K39/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/517 , A61K2039/5258 , A61K2039/543 , A61K2039/55505 , A61K2039/55583 , A61K2039/58 , C07K14/005 , C12N7/00 , C12N15/8257 , C12N15/8258 , C12N2760/16122 , C12N2760/16123 , C12N2760/16134
摘要： A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA of type A/California/04/09 in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein of type A/California/04/09 and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA of type A/California/04/09 as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译：提供了在植物或植物的一部分内合成流感病毒样颗粒（VLP）的方法。该方法包括在植物中表达A / California / 04/09型流感病毒HA，并通过大小排阻色谱法纯化。本发明还涉及包含A / California / 04/09型流感HA蛋白和植物脂质的VLP。本发明还涉及编码A / California / 04/09型流感病毒的核酸以及载体。 VLP可用于制定流感疫苗，或可用于丰富现有疫苗。

2. 发明申请

US20130295609A1 PLANT EXPRESSION SYSTEM 审中-公开
标题翻译：植物表达系统
公开(公告)号：US20130295609A1
公开(公告)日：2013-11-07
申请号：US13883439
申请日：2011-11-03
申请人： Marc-Andre D'Aoust , Pierre-Olivier Lavoie , Louis-Philippe Vezina
发明人： Marc-Andre D'Aoust , Pierre-Olivier Lavoie , Louis-Philippe Vezina
IPC分类号： C07K14/005
CPC分类号： C07K14/005 , C12N15/8203 , C12N15/8216 , C12N15/8221 , C12N15/8257 , C12N15/8258 , C12N2750/12022 , C12N2760/16051 , C12N2770/18022 , C12N2820/60 , C12N2830/60
摘要： A plant expression system and methods for expressing a protein of interest in a plant are provided. The plant expression system comprises a first nucleic acid sequence regulatory region sequence, operatively linked with a one or more than one comovirus enhancer, a nucleotide sequence of interest, one or more than one geminivirus amplification elements, and a second nucleic acid encoding a geminivirus replicase. The method of producing a protein of interest in a plant, involves introducing the plant expression system into a plant, or portion of the plant, and incubating the plant or the portion of the plant under conditions that permit the expression of the nucleotide sequence and producing the protein of interest.
摘要翻译：提供植物表达系统和在植物中表达目的蛋白质的方法。植物表达系统包含第一核酸序列调控区序列，其与一个或多于一个的病毒增强子，目的核苷酸序列，一个或多于一个的双核病毒扩增元件可操作地连接，以及编码双重病毒复制子的第二个核酸。在植物中生产感兴趣的蛋白质的方法包括将植物表达系统引入植物或植物的一部分，并且在允许表达核苷酸序列并产生感兴趣的蛋白质

3. 发明申请

US20110008837A1 MODIFYING GLYCOPROTEIN PRODUCTION IN PLANS 审中-公开
标题翻译：修改计划中的糖蛋白生产
公开(公告)号：US20110008837A1
公开(公告)日：2011-01-13
申请号：US12664567
申请日：2008-06-13
申请人： Marc-Andre D-Aoust , Louis-Philippe Vezina , Stephanie Aquin , Estelle Marquet-Blouin , Muriel Bardor , Veronique Gomord , Christophe Rihouey , Thomas Paccalet , Carole Burel , Loic Faye , Patrice Lerouge , Christophe Sourrouille
发明人： Marc-Andre D-Aoust , Louis-Philippe Vezina , Stephanie Aquin , Estelle Marquet-Blouin , Muriel Bardor , Veronique Gomord , Christophe Rihouey , Thomas Paccalet , Carole Burel , Loic Faye , Patrice Lerouge , Christophe Sourrouille
IPC分类号： C12P21/04 , C12P21/00 , C07H21/04 , C12N5/10 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8257 , C12N9/1051 , C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8258
摘要： A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNT1-GalT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNT1) fused to a catalytic domain of beta-1,4galactosyltransferase (GalT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.
摘要翻译：提供了在植物，植物的一部分或植物细胞内合成具有修饰的N-糖基化谱的目的蛋白质的方法。该方法包括在植物中共表达编码编码杂合蛋白（GNT1-GalT）的第一核苷酸序列的核苷酸序列，所述第一核苷酸序列包含与β-1,4-半乳糖基转移酶的催化结构域融合的N-乙酰葡糖胺基转移酶（GNT1）的CTS结构域 GalT），与在植物中有活性的第一调节区可操作地连接的第一核苷酸序列和用于编码目的蛋白质的第二核苷酸序列，第二核苷酸序列与在植物中有活性的第二调节区可操作地连接。共表达第一和第二核苷酸序列以合成目标蛋白，其包含在植物，植物部分或植物细胞内具有修饰的N-糖基化谱的聚糖。

4. 发明申请

US20090312240A1 Synthesis of Sialic Acid in Plants 有权
标题翻译：植物中唾液酸的合成
公开(公告)号：US20090312240A1
公开(公告)日：2009-12-17
申请号：US12278677
申请日：2007-02-09
申请人： Thomas Paccalet , Muriel Bardor , Chirstophe Rihouey , Véronique Gomord , Loïc Faye , Patrice Lerouge , Stéphanie Aquin , Louis-Philippe Vezina , Marc-André D'Aoust
发明人： Thomas Paccalet , Muriel Bardor , Chirstophe Rihouey , Véronique Gomord , Loïc Faye , Patrice Lerouge , Stéphanie Aquin , Louis-Philippe Vezina , Marc-André D'Aoust
IPC分类号： A61K38/16 , C12P19/26 , C12P21/06 , A01H5/00 , C12N5/10 , A01H5/10 , C12N15/82
CPC分类号： C12N15/8245 , C12N9/1085 , C12N9/88 , C12N15/8257 , C12P19/26 , C12P21/005 , C12Y205/01057 , C12Y401/03003
摘要： A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated a protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, CMP-Neu5Ac transporter and a sialyltransferase.
摘要翻译：提供了一种在植物中合成唾液酸的方法，以及能够合成唾液酸的植物。此外，还提供了在植物中产生唾液酸化蛋白质的方法。合成唾液酸的方法包括提供包含编码N-乙酰神经氨酸（Neu5Ac）合酶或Neu5Ac裂解酶的核苷酸序列的植物，并表达该核苷酸序列从而合成唾液酸。植物还可以共表达编码差向异构酶，CMP-Neu5Ac合成酶，CMP-Neu5Ac转运蛋白和唾液酸转移酶中的一种或多种的核苷酸序列。

5. 发明授权

US09815873B2 Method for recovering plant-derived proteins 有权
公开(公告)号：US09815873B2
公开(公告)日：2017-11-14
申请号：US14006552
申请日：2012-03-23
申请人： Manon Couture , Dany Paquet , Louis-Philippe Vezina
发明人： Manon Couture , Dany Paquet , Louis-Philippe Vezina
IPC分类号： C07K14/415 , C07K1/14
CPC分类号： C07K14/415 , C07K1/145
摘要： Methods of recovering plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, loosening the cell wall to produce a plant or plant matter having a loosened cell wall, thus allowing the release of apoplastic content through the cell wall to produce an apoplastic content fraction from the plant or plant matter, and recovering the apoplastic content fraction. The apoplastic content fraction comprises plant-derived proteins or suprastructure proteins.

6. 发明申请

US20130067807A1 METHOD OF PREPARING PLANT-DERIVED PROTEINS 审中-公开
标题翻译：制备植物衍生蛋白的方法
公开(公告)号：US20130067807A1
公开(公告)日：2013-03-21
申请号：US13497767
申请日：2010-09-21
申请人： Louis-Philippe Vezina , Manon Couture , Dany Paquet , Michele Dargis , Marc-Andre D'Aoust
发明人： Louis-Philippe Vezina , Manon Couture , Dany Paquet , Michele Dargis , Marc-Andre D'Aoust
IPC分类号： C07K1/14 , A01D45/00 , A01G1/00 , A01C11/00 , C12S3/14 , C12N15/82
摘要： Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.
摘要翻译：提供了制备植物衍生蛋白或超基质蛋白的方法。该方法可以包括获得植物或包含质外体蛋白质定位蛋白质的植物物质或超基质蛋白，从植物或植物物质产生原生质体/原生质体级分和质外体级分，并回收载脂蛋白级分。载脂蛋白级分包含植物来源的蛋白质或超基因蛋白质。或者，可以通过使用细胞壁降解酶组合物消化植物物质以产生消化部分，从包含植物衍生的蛋白质或超基质蛋白质的植物或植物物质获得蛋白质或超基质蛋白质。将消化的馏分过滤以产生过滤的馏分，从过滤的馏分中回收植物来源的蛋白质或超结构蛋白质。

7. 发明申请

US20120189658A1 Chimeric Influenza Virus-Like Particles Comprising Hemagglutinin 审中-公开
标题翻译：包含血凝素的嵌合型流感病毒样颗粒
公开(公告)号：US20120189658A1
公开(公告)日：2012-07-26
申请号：US13380346
申请日：2010-06-25
申请人： Manon Couture , Michele Dargis , Pierre-Olivier Lavoie , Louis-Philippe Vezina , Marc-Andre D'Aoust
发明人： Manon Couture , Michele Dargis , Pierre-Olivier Lavoie , Louis-Philippe Vezina , Marc-Andre D'Aoust
IPC分类号： A61K39/145 , A61P31/16 , A01H5/00 , A61P37/04 , C07K14/11 , C12N15/44
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5258 , C07K14/005 , C07K2319/00 , C07K2319/02 , C12N15/8257 , C12N2760/16122 , C12N2760/16123 , C12N2760/16134 , C12N2760/16222 , C12N2760/16223 , C12N2760/16234
摘要： A method for synthesizing chimeric influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of chimeric influenza HA in a plant or a portion of a plant. The invention is also directed towards a VLP comprising chimeric influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding chimeric influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译：提供了一种在植物或植物的一部分内合成嵌合型流感病毒样颗粒（VLP）的方法。该方法包括在植物或植物的一部分中表达嵌合流感HA。本发明还涉及包含嵌合流感HA蛋白和植物脂质的VLP。本发明还涉及编码嵌合流感HA以及载体的核酸。 VLP可用于制定流感疫苗，或可用于丰富现有疫苗。

8. 发明申请

US20110312095A1 METHOD AND CONSTRUCTS FOR INCREASING RECOMBINANT PROTEIN PRODUCTION IN PLANTS DEHYDRATION STRESS 审中-公开
标题翻译：增加植物脱水胁迫中重组蛋白生产的方法和建议
公开(公告)号：US20110312095A1
公开(公告)日：2011-12-22
申请号：US12811903
申请日：2009-01-09
申请人： Serge Laberge , Souad Azelmat , Marc-Andre D'Aoust , Pierre-Olivier Lavoie , Jean Cloutier , Yves Castonguay , Louis-Philippe Vezina
发明人： Serge Laberge , Souad Azelmat , Marc-Andre D'Aoust , Pierre-Olivier Lavoie , Jean Cloutier , Yves Castonguay , Louis-Philippe Vezina
IPC分类号： C12N15/82 , C12N15/113
CPC分类号： C12N15/8237
摘要： The present invention provides methods and constructs for increasing recombinant protein production in plants during dehydration stress. The invention includes nucleic acids, inducible expression systems, and methods for using same for increasing recombinant protein production in plants and plant protection.
摘要翻译：本发明提供了用于在脱水胁迫期间增加植物中重组蛋白质生产的方法和构建体。本发明包括核酸，诱导型表达系统及其用于增加植物中重要蛋白质生产和植物保护的方法。

9. 发明申请

US20090181460A1 Bipartite System, Method and Composition for the Constitutive and Inducible Expression of High Levels of Foreign Proteins in Plants 有权
标题翻译：用于植物中高水平外源蛋白的组成型和诱导表达的双系统，方法和组成
公开(公告)号：US20090181460A1
公开(公告)日：2009-07-16
申请号：US12300922
申请日：2006-05-22
申请人： George Lomonossoff , Li Liu , Carmen Canizares , Marc-Andre D'Aoust , Louis-Philippe Vezina
发明人： George Lomonossoff , Li Liu , Carmen Canizares , Marc-Andre D'Aoust , Louis-Philippe Vezina
IPC分类号： C12N15/82 , C12N15/63 , A01H5/00 , C12N5/04
CPC分类号： C12N15/8203 , C12N15/8218 , C12N15/8257 , C12N15/8258
摘要： This invention comprises a combined transgene/virus vector system for the expression of heterologous proteins in plants. While exemplified with respect to the bipartite RNA plant virus, Cowpect mosaic virus (CPMV), other bipartite viral systems may be used to advantage according to the method, system and composition described in detail herein.
摘要翻译：本发明包括用于在植物中表达异源蛋白质的组合的转基因/病毒载体系统。尽管关于两部分RNA植物病毒的例证，根据本文详细描述的方法，系统和组合，可以使用考威花叶病毒（CPMV），其他二分之一病毒系统。

10. 发明授权

US08771703B2 Soluble recombinant influenza antigens 有权
标题翻译：可溶性重组流感抗原
公开(公告)号：US08771703B2
公开(公告)日：2014-07-08
申请号：US13001111
申请日：2009-07-07
申请人： Manon Couture , Nathalie Landry , Louis-Philippe Vezina , Michéle Dargis
发明人： Manon Couture , Nathalie Landry , Louis-Philippe Vezina , Michéle Dargis
IPC分类号： A61K39/145 , A61K39/00 , C12N15/82 , C12N15/79 , C12N15/84 , C12N15/44 , C12N15/62 , C12N15/90 , C12N5/10 , C07K14/005
CPC分类号： C12N15/8257 , A61K39/00 , A61K39/12 , A61K39/145 , C07K14/005 , C07K2319/50 , C07K2319/70 , C07K2319/73 , C07K2319/735 , C12N2760/16122 , C12N2760/16134
摘要： The present invention provides a recombinant soluble trimeric hemagglutinin (rHA) protein comprising a hemagglutinin ectodomain and an oligomerization domain. The rHA is produced as a soluble homotrimer, and may further comprises a signal peptide and/or an endoplasmic reticulum (ER) retention signal. The invention is also directed to nucleic acids encoding the rHA of the invention, as well as vectors and chimeric constructs comprising the nucleic acid. Methods of producing the rHA are also provided. The rHA described herein may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译：本发明提供了包含血凝素胞外域和寡聚化结构域的重组可溶性三聚体血凝素（rHA）蛋白。 rHA产生为可溶性同三聚物，并且还可以包含信号肽和/或内质网（ER）保留信号。本发明还涉及编码本发明的rHA的核酸，以及包含该核酸的载体和嵌合构建体。还提供了生产rHA的方法。本文所述的rHA可用于制定流感疫苗，或可用于富集现有疫苗。

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