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    • 3. 发明申请
    • PROTEIN SUBSTRATE AND ITS MANUFACTURING METHOD
    • 蛋白质底物及其制造方法
    • US20130109036A1
    • 2013-05-02
    • US13284812
    • 2011-10-28
    • YI-LING YELI-SHIAN SHILIN-YI WUI-EN LINJENG-HAUR HORNGL. JAMES LEE
    • YI-LING YELI-SHIAN SHILIN-YI WUI-EN LINJENG-HAUR HORNGL. JAMES LEE
    • G01N33/53C12P21/00
    • G01N33/54353
    • A protein substrate includes a base having micro-passages and reaction grooves, a polyethyleneimine (PEI) fixed on the base and a specific protein fixed on the PEI. The base is modified by plasma stripper to make the PEI bonded on the base. Mixed with tyrosinase, the specific protein can stably stick on the base due to the tyrosinase bonded with the PEI. By antigen-antibody bonding specificity, the invention can quickly detect antibody in a sample able to link with the specific protein. The specific antibody can be added with targeted biotin secondary antibody, targeted avidin-peroxidase, and color producing substrate able to react with the targeted avidin-peroxidase, such as tetramethylbenzidine or 3-(4-hydroxy)phenyl propionic acid (HPPA), so as to measure a specific antibody amount.
    • 蛋白质底物包括具有微通道和反应槽的基底,固定在基底上的聚乙烯亚胺(PEI)和固定在PEI上的特定蛋白质。 基体由等离子体汽提器改性,使PEI结合在基体上。 与酪氨酸酶混合,由于与PEI结合的酪氨酸酶,特异性蛋白质可以稳定地粘在基质上。 通过抗原 - 抗体结合特异性,本发明可以快速检测能够与特异性蛋白质连接的样品中的抗体。 特异性抗体可以加入靶向的生物素二抗,靶向的抗生物素蛋白 - 过氧化物酶和能够与靶向的抗生物素蛋白 - 过氧化物酶如四甲基联苯胺或3-(4-羟基)苯基丙酸(HPPA)反应的产生色素的底物,因此 以测量特异性抗体量。