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1. 发明申请

US20080187966A1 Antibodies and methods for making and using them 审中-公开
标题翻译：用于制造和使用它们的抗体和方法
公开(公告)号：US20080187966A1
公开(公告)日：2008-08-07
申请号：US11928201
申请日：2007-10-30
申请人： Laura Simmons
发明人： Laura Simmons
IPC分类号： C12N15/09 , C07K16/00
CPC分类号： C07K16/22 , C07K16/00 , C07K16/42 , C07K16/4291 , C07K2317/567
摘要： The present invention provides methods for producing humanized antibodies and increasing the yield of antibodies and/or antigen binding fragments when produced in cell culture. In one aspect of the invention, at least one framework region amino acid residue of the variable domain is substituted by a corresponding amino acid from a variable domain consensus sequence subgroup that has the most sequence identity with the HVR1 and/or HVR2 amino acid sequence of the variable domain. In another aspect, an amino acid is placed at a position proximal to a cys residue that participates in an intrachain variable domain disulfide bond that corresponds to an amino acid found at that position in a variable domain consensus sequence subgroup that has the most sequence identity with the HVR1 and/or HVR2 amino acid sequence of the variable domain.
摘要翻译：本发明提供了当在细胞培养物中产生时产生人源化抗体并增加抗体和/或抗原结合片段的产量的方法。在本发明的一个方面，可变结构域的至少一个框架区氨基酸残基被来自与HVR1和/或HVR2氨基酸序列具有最高序列同一性的可变结构域共有序列亚组的相应氨基酸取代可变域。在另一方面，将氨基酸置于接近cys残基的位置，所述cys残基参与相应于在该位置上发现的氨基酸的可变结构域共有序列亚组中具有最多序列同一性的氨基酸的链内可变结构域二硫键可变结构域的HVR1和/或HVR2氨基酸序列。

2. 发明申请

US20120077740A1 Methods for making Apo-2 Ligand using divalent metal ions 审中-公开
标题翻译：使用二价金属离子制备Apo-2配体的方法
公开(公告)号：US20120077740A1
公开(公告)日：2012-03-29
申请号：US12928422
申请日：2010-12-10
申请人： Avi J. Ashkenazi , Sarah Hymowitz , Robert F. Kelley , Iphigenia Koumenis , Woon-Lam Susan Leung , Mark O'Connell , Roger Pai , Zahra Shahrokh , Laura Simmons
发明人： Avi J. Ashkenazi , Sarah Hymowitz , Robert F. Kelley , Iphigenia Koumenis , Woon-Lam Susan Leung , Mark O'Connell , Roger Pai , Zahra Shahrokh , Laura Simmons
IPC分类号： A61K38/19 , C12N15/19 , C12N15/63 , C12N1/21 , B01D15/36 , C07K1/14 , C07K1/18 , C07K1/20 , B01D15/32 , C12P21/02 , C07K14/52
CPC分类号： A61K9/19 , A61K9/0019 , A61K38/00 , A61K47/02 , C07K14/525 , C07K14/70575
摘要： Methods of making Apo-2 ligand and formulations of Apo-2 ligand using divalent metal ions are provided. Such divalent metal ions include zinc and cobalt which improve Apo-2 ligand trimer formation and stability. The crystal structure of Apo-2 ligand is also provided, along with Apo-2 ligand variant polypeptides identified using oligonucleotide-directed mutagenesis.
摘要翻译：提供使用二价金属离子制备Apo-2配体和Apo-2配体制剂的方法。这种二价金属离子包括改善Apo-2配体三聚体形成和稳定性的锌和钴。还提供了Apo-2配体的晶体结构，以及使用寡核苷酸定向突变鉴定的Apo-2配体变体多肽。

3. 发明授权

US07575893B2 Methods for producing humanized antibodies and improving yield of antibodies or antigen binding fragments in cell culture 有权
标题翻译：生产人源化抗体的方法，提高细胞培养中抗体或抗原结合片段的产量
公开(公告)号：US07575893B2
公开(公告)日：2009-08-18
申请号：US10764428
申请日：2004-01-23
申请人： Laura Simmons
发明人： Laura Simmons
IPC分类号： C12N1/21 , C12N15/06 , C12N15/03 , C12N15/13 , C07K16/22
CPC分类号： C07K16/22 , C07K16/00 , C07K16/42 , C07K16/4291 , C07K2317/567
摘要： The present invention provides methods for producing humanized antibodies and increasing the yield of antibodies and/or antigen binding fragments when produced in cell culture. In one aspect of the invention, at least one framework region amino acid residue of the variable domain is substituted by a corresponding amino acid from a variable domain consensus sequence subgroup that has the most sequence identity with the HVR1 and/or HVR2 amino acid sequence of the variable domain. In another aspect, an amino acid is placed at a position proximal to a cys residue that participates in an intrachain variable domain disulfide bond that corresponds to an amino acid found at that position in a variable domain consensus sequence subgroup that has the most sequence identity with the HVR1 and/or HVR2 amino acid sequenc of the variable domain.
摘要翻译：本发明提供了当在细胞培养物中产生时产生人源化抗体并增加抗体和/或抗原结合片段的产量的方法。在本发明的一个方面，可变结构域的至少一个框架区氨基酸残基被来自与HVR1和/或HVR2氨基酸序列具有最高序列同一性的可变结构域共有序列亚组的相应氨基酸取代可变域。在另一方面，将氨基酸置于接近cys残基的位置，所述cys残基参与相应于在该位置上发现的氨基酸的可变结构域共有序列亚组中具有最多序列同一性的氨基酸的链内可变结构域二硫键可变结构域的HVR1和/或HVR2氨基酸序列。

4. 发明申请

US20070224664A1 System for Antibody Expression and Assembly 审中-公开
标题翻译：抗体表达和装配体系
公开(公告)号：US20070224664A1
公开(公告)日：2007-09-27
申请号：US11627071
申请日：2007-01-25
申请人： Laura Simmons , Dana Anderson
发明人： Laura Simmons , Dana Anderson
IPC分类号： C12P21/02 , C12N1/21 , C12N15/00
CPC分类号： C07K16/36 , C07K16/00 , C07K16/2845 , C07K16/2896 , C07K2317/54
摘要： The present invention provides methods and compositions for expression and production of recombinant antibodies in a host cell system, such as prokaryotic and eukaryotic expression systems. Particularly contemplated are recombinant systems for temporally separated expression of light chain and heavy chain of antibodies. The antibody products including antibody fragments can be used in various aspects of biological research, diagnosis and medical treatment.
摘要翻译：本发明提供用于在宿主细胞系统中表达和产生重组抗体的方法和组合物，例如原核和真核表达系统。特别考虑的是用于时间分离表达轻链和重链抗体的重组系统。包括抗体片段的抗体产物可用于生物研究，诊断和治疗的各个方面。

5. 发明申请

US20070015244A1 System for antibody expression and assembly 审中-公开
标题翻译：用于抗体表达和组装的系统
公开(公告)号：US20070015244A1
公开(公告)日：2007-01-18
申请号：US11438484
申请日：2006-05-22
申请人： Laura Simmons , Dana Andersen
发明人： Laura Simmons , Dana Andersen
IPC分类号： C12P21/06 , C07H21/04 , C12N1/21 , C07K16/18
CPC分类号： C07K16/36 , C07K16/00 , C07K16/2845 , C07K16/2896 , C07K2317/54
摘要： The present invention provides methods and compositions for expression and production of recombinant antibodies in a host cell system, such as prokaryotic and eukaryotic expression systems. Particularly contemplated are recombinant systems for temporally separated expression of light chain and heavy chain of antibodies. The antibody products including antibody fragments can be used in various aspects of biological research, diagnosis and medical treatment.
摘要翻译：本发明提供用于在宿主细胞系统中表达和产生重组抗体的方法和组合物，例如原核和真核表达系统。特别考虑的是用于时间分离表达轻链和重链抗体的重组系统。包括抗体片段的抗体产物可用于生物研究，诊断和治疗的各个方面。

6. 发明授权

US07855066B1 Methods for making Apo-2 ligand using divalent metal ions 有权
标题翻译：使用二价金属离子制备Apo-2配体的方法
公开(公告)号：US07855066B1
公开(公告)日：2010-12-21
申请号：US11528948
申请日：2006-09-28
申请人： Avi J. Ashkenazi , Sarah Hymowitz , Robert F. Kelley , Iphigenia Koumenis , Woon-Lam Susan Leung , Mark O'Connell , Roger Pai , Zahra Shahrokh , Laura Simmons
发明人： Avi J. Ashkenazi , Sarah Hymowitz , Robert F. Kelley , Iphigenia Koumenis , Woon-Lam Susan Leung , Mark O'Connell , Roger Pai , Zahra Shahrokh , Laura Simmons
IPC分类号： C12N5/10 , C12N15/12 , C07K14/52
CPC分类号： A61K9/19 , A61K9/0019 , A61K38/00 , A61K47/02 , C07K14/525 , C07K14/70575
摘要： Methods of making Apo-2 ligand and formulations of Apo-2 ligand using divalent metal ions are provided. Such divalent metal ions include zinc and cobalt which improve Apo-2 ligand trimer formation and stability. The crystal structure of Apo-2 ligand is also provided, along with Apo-2 ligand variant polypeptides identified using oligonucleotide-directed mutagenesis.
摘要翻译：提供使用二价金属离子制备Apo-2配体和Apo-2配体制剂的方法。这种二价金属离子包括改善Apo-2配体三聚体形成和稳定性的锌和钴。还提供了Apo-2配体的晶体结构，以及使用寡核苷酸定向突变鉴定的Apo-2配体变体多肽。

7. 发明申请

US20070161564A1 Methods for making Apo-2 ligand using divalent metal ions 审中-公开
标题翻译：使用二价金属离子制备Apo-2配体的方法
公开(公告)号：US20070161564A1
公开(公告)日：2007-07-12
申请号：US11603584
申请日：2006-11-22
申请人： Avi Ashkenazi , Sarah Hymowitz , Robert Kelley , Iphigenia Koumenis , Woon-Lam Leung , Mark O'Connell , Roger Pai , Zahra Shahrokh , Laura Simmons
发明人： Avi Ashkenazi , Sarah Hymowitz , Robert Kelley , Iphigenia Koumenis , Woon-Lam Leung , Mark O'Connell , Roger Pai , Zahra Shahrokh , Laura Simmons
IPC分类号： A61K38/17
CPC分类号： A61K9/19 , A61K9/0019 , A61K38/00 , A61K47/02 , C07K14/525 , C07K14/70575
摘要： Methods of making Apo-2 ligand and formulations of Apo-2 ligand using divalent metal ions are provided. Such divalent metal ions include zinc and cobalt which improve Apo-2 ligand trimer formation and stability. The crystal structure of Apo-2 ligand is also provided, along with Apo-2 ligand variant polypeptides identified using oligonucleotide-directed mutagenesis.
摘要翻译：提供使用二价金属离子制备Apo-2配体和Apo-2配体制剂的方法。这种二价金属离子包括改善Apo-2配体三聚体形成和稳定性的锌和钴。还提供了Apo-2配体的晶体结构，以及使用寡核苷酸定向突变鉴定的Apo-2配体变体多肽。

8. 发明申请

US20060170728A1 Pen wiping method and system that employs a treadmill belt 失效
标题翻译：使用跑步机皮带的笔擦拭方法和系统
公开(公告)号：US20060170728A1
公开(公告)日：2006-08-03
申请号：US11391095
申请日：2006-03-27
申请人： Laura Simmons , Jonathan Andrews , Stephen Cooper
发明人： Laura Simmons , Jonathan Andrews , Stephen Cooper
IPC分类号： B41J2/165
CPC分类号： B41J2/16547 , B41J23/025
摘要： According to one embodiment of the present invention, a service station for maintaining pens is provided. The service station includes a treadmill belt that has an outer surface and an inner surface. At least one wiper is positioned on the outer surface of the treadmill belt. The treadmill belt is mounted on a first roller and a second roller. The first roller and the second roller contact the inner surface of the treadmill belt for driving the treadmill belt. Either the first roller or the second roller can be coupled to a drive axle. Preferably, the drive axle is coupled to a paper motor via a paper drive shaft. The wiper can be, for example, a flicker squeegee or a wicking squeegee.
摘要翻译：根据本发明的一个实施例，提供了一种用于维持笔的维修站。维修站包括具有外表面和内表面的跑步机带。至少一个擦拭器位于跑步机皮带的外表面上。跑步机皮带安装在第一辊和第二辊上。第一辊和第二辊接触跑步机带的内表面以驱动跑步机带。第一辊或第二辊可以联接到驱动轴。优选地，驱动轴通过纸驱动轴联接到纸马达。擦拭器可以是例如闪光刮板或吸液刮板。

9. 发明申请

US20110244517A1 SYSTEM FOR ANTIBODY EXPRESSION AND ASSEMBLY 审中-公开
标题翻译：用于抗体表达和组装的系统
公开(公告)号：US20110244517A1
公开(公告)日：2011-10-06
申请号：US13033521
申请日：2011-02-23
申请人： Laura Simmons , Dana Andersen
发明人： Laura Simmons , Dana Andersen
IPC分类号： C12P21/00 , C12N1/21 , C12N15/63
CPC分类号： C07K16/36 , C07K16/00 , C07K16/2845 , C07K16/2896 , C07K2317/54
摘要： The present invention provides methods and compositions for expression and production of recombinant antibodies in a host cell system, such as prokaryotic and eukaryotic expression systems. Particularly contemplated are recombinant systems for temporally separated expression of light chain and heavy chain of antibodies. The antibody products including antibody fragments can be used in various aspects of biological research, diagnosis and medical treatment.
摘要翻译：本发明提供用于在宿主细胞系统中表达和产生重组抗体的方法和组合物，例如原核和真核表达系统。特别考虑的是用于时间分离表达轻链和重链抗体的重组系统。包括抗体片段的抗体产物可用于生物研究，诊断和治疗的各个方面。

10. 发明申请

US20070065909A1 Prokaryotically produced antibodies and uses thereof 审中-公开
公开(公告)号：US20070065909A1
公开(公告)日：2007-03-22
申请号：US11351905
申请日：2006-02-09
申请人： Laura Simmons , Laura Klimowski , Dorothea Reilly , Daniel Yansura
发明人： Laura Simmons , Laura Klimowski , Dorothea Reilly , Daniel Yansura
IPC分类号： C07K16/44 , C07H21/04 , C12N15/74 , C12P21/08 , C12N1/21
CPC分类号： C07K16/36 , A61K2039/505 , C07K16/00 , C07K16/22 , C07K16/2845 , C07K16/2878 , C07K16/2896 , C07K16/32 , C07K16/4291 , C07K2317/20 , C07K2317/41 , C07K2317/52 , C07K2317/55 , C07K2319/00 , C12N15/70
摘要： The present invention provides methods and compositions for improved expression and production of recombinant antibodies in prokaryotic expression systems. Particularly contemplated are prokaryotic expression and production of full length aglycosylated antibodies. The antibody products of the invention can be used in various aspects of biological research, diagnosis and medical treatment.

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