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1. 发明申请

US20050153440A1 Methods for assaying gene imprinting and methylated cpg islands 审中-公开
标题翻译：测定基因印迹和甲基化cpg岛的方法
公开(公告)号：US20050153440A1
公开(公告)日：2005-07-14
申请号：US10296263
申请日：2001-05-22
申请人： Andrew Feinberg , Liora Strichman-Almashanu , Shan Jiang
发明人： Andrew Feinberg , Liora Strichman-Almashanu , Shan Jiang
IPC分类号： A01K67/027 , A61P35/00 , C12N5/074 , C12N5/10 , C12N15/09 , C12Q1/02 , C12Q1/68 , C12N5/06
CPC分类号： C12N5/0611 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2501/385 , C12N2502/13 , C12N2503/00 , C12N2503/02 , C12N2510/00 , C12N2517/00 , C12Q1/6827 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136 , C12Q2600/154
摘要： Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.
摘要翻译：基因印记是起源依赖基因沉默的亲本，其涉及在种系中标记等位基因和在后代的体细胞中的差异表达。印迹基因和异常印记涉及发育，人类疾病和胚胎干细胞移植。我们已经建立了使用多能8.5 d.p.c的基因组印迹模型系统。来自种间交叉的小鼠胚胎胚芽（EG）细胞系。我们发现在这些细胞中已经丢失了等位基因特异性印记的基因表达。然而，在将EG细胞体外分化成体细胞谱系后，一些印迹基因可能发生等位基因特异性沉默的部分恢复，表明存在可与等位基因特异性基因沉默分离的配子记忆。我们还生成了一个包含大多数甲基化CpG岛的文库。分析了这些克隆的一个子集，并揭示了甲基化CpG岛分为4个不同的亚型：属于高拷贝数重复家族的CpG岛; 所有组织中独特的CpG岛甲基化; 在父系种系中未甲基化的独特的甲基化CpG岛; 和独特的CpG岛在肿瘤中甲基化。该方法鉴定了整个基因组中甲基化CpG岛的甲基化。

2. 发明授权

US07488815B2 Gene imprinting and methylated CpG islands 失效
标题翻译：基因印迹和甲基化CpG岛
公开(公告)号：US07488815B2
公开(公告)日：2009-02-10
申请号：US11084085
申请日：2005-03-17
申请人： Andrew P. Feinberg , Liora Strichman-Almashanu , Shan Jiang
发明人： Andrew P. Feinberg , Liora Strichman-Almashanu , Shan Jiang
IPC分类号： C07H21/04
CPC分类号： C12N5/0611 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2501/385 , C12N2502/13 , C12N2503/00 , C12N2503/02 , C12N2510/00 , C12N2517/00 , C12Q1/6827 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136 , C12Q2600/154
摘要： Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.
摘要翻译：基因印记是起源依赖基因沉默的亲本，其涉及在种系中标记等位基因和在后代的体细胞中的差异表达。印迹基因和异常印记涉及发育，人类疾病和胚胎干细胞移植。我们已经建立了使用多能8.5 d.p.c的基因组印迹模型系统。来自种间交叉的小鼠胚胎胚芽（EG）细胞系。我们发现在这些细胞中已经丢失了等位基因特异性印记的基因表达。然而，在将EG细胞体外分化成体细胞谱系后，一些印迹基因可能发生等位基因特异性沉默的部分恢复，表明存在可与等位基因特异性基因沉默分离的配子记忆。我们还生成了一个包含大多数甲基化CpG岛的文库。分析了这些克隆的一个子集，并揭示了甲基化CpG岛分为4个不同的亚型：属于高拷贝数重复家族的CpG岛; 所有组织中独特的CpG岛甲基化; 在父系种系中未甲基化的独特的甲基化CpG岛; 和独特的CpG岛在肿瘤中甲基化。该方法鉴定了整个基因组中甲基化CpG岛的甲基化。

3. 发明申请

US20100273658A1 Methods for Assaying Gene Imprinting and Methylated CpG Islands 失效
标题翻译：测定基因印迹和甲基化CpG群岛的方法
公开(公告)号：US20100273658A1
公开(公告)日：2010-10-28
申请号：US12350159
申请日：2009-01-07
申请人： ANDREW P. FEINBERG , LIORA STRICHMAN-ALMASHANU , SHAN JIANG
发明人： ANDREW P. FEINBERG , LIORA STRICHMAN-ALMASHANU , SHAN JIANG
IPC分类号： C40B20/00 , C12N5/073 , C12Q1/68 , C40B50/00
CPC分类号： C12N5/0611 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2501/385 , C12N2502/13 , C12N2503/00 , C12N2503/02 , C12N2510/00 , C12N2517/00 , C12Q1/6827 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136 , C12Q2600/154
摘要： Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.
摘要翻译：基因印记是起源依赖基因沉默的亲本，其涉及在种系中标记等位基因和在后代的体细胞中的差异表达。印迹基因和异常印记涉及发育，人类疾病和胚胎干细胞移植。我们已经建立了使用多能8.5 d.p.c的基因组印迹模型系统。来自种间交叉的小鼠胚胎胚芽（EG）细胞系。我们发现在这些细胞中已经丢失了等位基因特异性印记的基因表达。然而，在将EG细胞体外分化成体细胞谱系后，一些印迹基因可能发生等位基因特异性沉默的部分恢复，表明存在可与等位基因特异性基因沉默分离的配子记忆。我们还生成了一个包含大多数甲基化CpG岛的文库。分析了这些克隆的一个子集，并揭示了甲基化CpG岛分为4个不同的亚型：属于高拷贝数重复家族的CpG岛; 所有组织中独特的CpG岛甲基化; 在父系种系中未甲基化的独特的甲基化CpG岛; 和独特的CpG岛在肿瘤中甲基化。该方法鉴定了整个基因组中甲基化CpG岛的甲基化。

4. 发明申请

US20050282147A1 Methods for assaying gene imprinting and methylated CpG islands 失效
标题翻译：测定基因印迹和甲基化CpG岛的方法
公开(公告)号：US20050282147A1
公开(公告)日：2005-12-22
申请号：US11084085
申请日：2005-03-17
申请人： Andrew Feinberg , Liora Strichman-Almashanu , Shan Jiang
发明人： Andrew Feinberg , Liora Strichman-Almashanu , Shan Jiang
IPC分类号： A01K67/027 , A61P35/00 , C12N5/074 , C12N5/10 , C12N15/09 , C12Q1/02 , C12Q1/68 , C12Q1/00 , C12N5/08
CPC分类号： C12N5/0611 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2501/385 , C12N2502/13 , C12N2503/00 , C12N2503/02 , C12N2510/00 , C12N2517/00 , C12Q1/6827 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136 , C12Q2600/154
摘要： Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.
摘要翻译：基因印记是起源依赖基因沉默的亲本，其涉及在种系中标记等位基因和在后代的体细胞中的差异表达。印迹基因和异常印记涉及发育，人类疾病和胚胎干细胞移植。我们已经建立了使用多能8.5 d.p.c的基因组印迹模型系统。来自种间交叉的小鼠胚胎胚芽（EG）细胞系。我们发现在这些细胞中已经丢失了等位基因特异性印记的基因表达。然而，在将EG细胞体外分化成体细胞谱系后，一些印迹基因可能发生等位基因特异性沉默的部分恢复，表明存在可与等位基因特异性基因沉默分离的配子记忆。我们还生成了一个包含大多数甲基化CpG岛的文库。分析了这些克隆的一个子集，并揭示了甲基化CpG岛分为4个不同的亚型：属于高拷贝数重复家族的CpG岛; 所有组织中独特的CpG岛甲基化; 在父系种系中未甲基化的独特的甲基化CpG岛; 和独特的CpG岛在肿瘤中甲基化。该方法鉴定了整个基因组中甲基化CpG岛的甲基化。

5. 发明授权

US07985550B2 Methods of isolating methylated CpG islands 失效
标题翻译：分离甲基化CpG岛的方法
公开(公告)号：US07985550B2
公开(公告)日：2011-07-26
申请号：US12350159
申请日：2009-01-07
申请人： Andrew P. Feinberg , Liora Strichman-Almashanu , Shan Jiang
发明人： Andrew P. Feinberg , Liora Strichman-Almashanu , Shan Jiang
IPC分类号： C12Q1/68
CPC分类号： C12N5/0611 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2501/385 , C12N2502/13 , C12N2503/00 , C12N2503/02 , C12N2510/00 , C12N2517/00 , C12Q1/6827 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136 , C12Q2600/154
摘要： Provided is a method for isolating methylated CpG islands. The method includes identifying an imprinted gene adjacent to the methylated CpG island, and identifying a disease which is preferentially transmitted by one parent and which is genetically linked to region of genomic DNA which contains the imprinted gene, whereby the imprinted gene is thereby indicated as a candidate gene involved in transmitting the disease.
摘要翻译：提供了分离甲基化CpG岛的方法。该方法包括鉴定与甲基化CpG岛相邻的印迹基因，以及鉴定由一个亲本优先传播并与遗传上连接到含有印迹基因的基因组DNA区域的疾病，从而将印迹基因表示为候选基因涉及传播疾病。

6. 发明授权

US06960434B2 Methods for assaying gene imprinting and methylated CpG islands 失效
公开(公告)号：US06960434B2
公开(公告)日：2005-11-01
申请号：US09861893
申请日：2001-05-22
申请人： Andrew P. Feinberg , Liora Strichman-Almashanu , Shan Jiang
发明人： Andrew P. Feinberg , Liora Strichman-Almashanu , Shan Jiang
IPC分类号： A01K67/027 , A61P35/00 , C12N5/074 , C12N5/10 , C12N15/09 , C12Q1/02 , C12Q1/68 , C12P19/34
CPC分类号： C12N5/0611 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2501/385 , C12N2502/13 , C12N2503/00 , C12N2503/02 , C12N2510/00 , C12N2517/00 , C12Q1/6827 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136 , C12Q2600/154
摘要： Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.

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