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1. 发明申请

US20050153440A1 Methods for assaying gene imprinting and methylated cpg islands 审中-公开
标题翻译：测定基因印迹和甲基化cpg岛的方法
公开(公告)号：US20050153440A1
公开(公告)日：2005-07-14
申请号：US10296263
申请日：2001-05-22
申请人： Andrew Feinberg , Liora Strichman-Almashanu , Shan Jiang
发明人： Andrew Feinberg , Liora Strichman-Almashanu , Shan Jiang
IPC分类号： A01K67/027 , A61P35/00 , C12N5/074 , C12N5/10 , C12N15/09 , C12Q1/02 , C12Q1/68 , C12N5/06
CPC分类号： C12N5/0611 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2501/385 , C12N2502/13 , C12N2503/00 , C12N2503/02 , C12N2510/00 , C12N2517/00 , C12Q1/6827 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136 , C12Q2600/154
摘要： Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.
摘要翻译：基因印记是起源依赖基因沉默的亲本，其涉及在种系中标记等位基因和在后代的体细胞中的差异表达。印迹基因和异常印记涉及发育，人类疾病和胚胎干细胞移植。我们已经建立了使用多能8.5 d.p.c的基因组印迹模型系统。来自种间交叉的小鼠胚胎胚芽（EG）细胞系。我们发现在这些细胞中已经丢失了等位基因特异性印记的基因表达。然而，在将EG细胞体外分化成体细胞谱系后，一些印迹基因可能发生等位基因特异性沉默的部分恢复，表明存在可与等位基因特异性基因沉默分离的配子记忆。我们还生成了一个包含大多数甲基化CpG岛的文库。分析了这些克隆的一个子集，并揭示了甲基化CpG岛分为4个不同的亚型：属于高拷贝数重复家族的CpG岛; 所有组织中独特的CpG岛甲基化; 在父系种系中未甲基化的独特的甲基化CpG岛; 和独特的CpG岛在肿瘤中甲基化。该方法鉴定了整个基因组中甲基化CpG岛的甲基化。

2. 发明申请

US20050282147A1 Methods for assaying gene imprinting and methylated CpG islands 失效
标题翻译：测定基因印迹和甲基化CpG岛的方法
公开(公告)号：US20050282147A1
公开(公告)日：2005-12-22
申请号：US11084085
申请日：2005-03-17
申请人： Andrew Feinberg , Liora Strichman-Almashanu , Shan Jiang
发明人： Andrew Feinberg , Liora Strichman-Almashanu , Shan Jiang
IPC分类号： A01K67/027 , A61P35/00 , C12N5/074 , C12N5/10 , C12N15/09 , C12Q1/02 , C12Q1/68 , C12Q1/00 , C12N5/08
CPC分类号： C12N5/0611 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2501/385 , C12N2502/13 , C12N2503/00 , C12N2503/02 , C12N2510/00 , C12N2517/00 , C12Q1/6827 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136 , C12Q2600/154
摘要： Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.
摘要翻译：基因印记是起源依赖基因沉默的亲本，其涉及在种系中标记等位基因和在后代的体细胞中的差异表达。印迹基因和异常印记涉及发育，人类疾病和胚胎干细胞移植。我们已经建立了使用多能8.5 d.p.c的基因组印迹模型系统。来自种间交叉的小鼠胚胎胚芽（EG）细胞系。我们发现在这些细胞中已经丢失了等位基因特异性印记的基因表达。然而，在将EG细胞体外分化成体细胞谱系后，一些印迹基因可能发生等位基因特异性沉默的部分恢复，表明存在可与等位基因特异性基因沉默分离的配子记忆。我们还生成了一个包含大多数甲基化CpG岛的文库。分析了这些克隆的一个子集，并揭示了甲基化CpG岛分为4个不同的亚型：属于高拷贝数重复家族的CpG岛; 所有组织中独特的CpG岛甲基化; 在父系种系中未甲基化的独特的甲基化CpG岛; 和独特的CpG岛在肿瘤中甲基化。该方法鉴定了整个基因组中甲基化CpG岛的甲基化。

3. 发明申请

US20050092191A1 Disposable barbeque smoking box with integrated soaking device 失效
公开(公告)号：US20050092191A1
公开(公告)日：2005-05-05
申请号：US10698362
申请日：2003-11-03
申请人： Andrew Feinberg
发明人： Andrew Feinberg
IPC分类号： A23L5/10 , A47J37/07 , B60H3/00
CPC分类号： A47J37/0786
摘要： A barbeque smoking box includes a metal container that encloses woodchips. Within the mass of the woodchips is located a frangible liquid container that ruptures when subjected to the heat of a barbeque grill. The liquid then permeates and soaks the woodchips in the frangible container. As the barbeque grill continues to heat the soaked woodchips, smoke is generated and passes in an continuous control manner through a number of small openings formed in the external package so as to smoke food that is being prepared in the grill.

4. 发明授权

US06935226B2 Disposable barbeque smoking box with integrated soaking device 失效
标题翻译：一次性烧烤吸烟箱，集成浸泡装置
公开(公告)号：US06935226B2
公开(公告)日：2005-08-30
申请号：US10698362
申请日：2003-11-03
申请人： Andrew Feinberg
发明人： Andrew Feinberg
IPC分类号： A23L5/10 , A47J37/07 , B60H3/00 , A23L1/01 , A23B4/04
CPC分类号： A47J37/0786
摘要： A barbeque smoking box includes a metal container that encloses woodchips. Within the mass of the woodchips is located a frangible liquid container that ruptures when subjected to the heat of a barbeque grill. The liquid then permeates and soaks the woodchips in the frangible container. As the barbeque grill continues to heat the soaked woodchips, smoke is generated and passes in an continuous control manner through a number of small openings formed in the external package so as to smoke food that is being prepared in the grill.
摘要翻译：烧烤箱包括一个封闭木片的金属容器。在木片的质量范围内是一个易碎的液体容器，当受烧烤烤架的热量破裂时。然后液体渗透并浸泡在易碎容器中的木片。当烧烤架继续加热浸泡的木片时，产生烟雾并以连续的控制方式通过形成在外部包装中的许多小开口，以便抽出在烤架中准备的食物。

5. 发明授权

US08394584B2 Detecting and sorting methylated DNA using a synthetic nanopore 有权
标题翻译：使用合成纳米孔检测和分选甲基化DNA
公开(公告)号：US08394584B2
公开(公告)日：2013-03-12
申请号：US13133300
申请日：2009-12-18
申请人： Gregory Timp , Winston Timp , Andrew Feinberg , Utkur Mirsaidov
发明人： Gregory Timp , Winston Timp , Andrew Feinberg , Utkur Mirsaidov
IPC分类号： C12Q1/68 , C12P19/34 , C12M1/34 , C07H21/02 , C07H19/00
CPC分类号： C12Q1/6827 , C12Q2565/631
摘要： Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.
摘要翻译：提供了用于基于甲基化检测，表征或分离DNA的方法。通过提供具有通过其施加电场的纳米孔的膜，基于DNA甲基化分离不均匀DNA群体。引入目标DNA，对于纳米孔上的给定阈值电压，只有具有目标甲基化参数的DNA才能转移孔，从而便于基于甲基化的DNA的检测，表征或分离。任选地，所述方法用于检测与DNA甲基化相关的疾病状态，包括但不限于癌症。

6. 发明申请

US20070090253A1 ADJUSTABLE CLAMP FOR CYLINDRICAL OBJECT 审中-公开
标题翻译：用于圆柱体的可调节夹具
公开(公告)号：US20070090253A1
公开(公告)日：2007-04-26
申请号：US11163664
申请日：2005-10-26
申请人： Andrew Feinberg
发明人： Andrew Feinberg
IPC分类号： F16B45/00
CPC分类号： A62C13/78
摘要： A clamp for a cylindrical object is adapted for mounting to a surface and includes a hose clamp having a metal strap for encircling a cylindrical object. The strap includes a plurality of spaced openings for meshing with a worm gear member rotationally mounted in a rider that is slidably positionable along the length of the strap. An over-center double bail is pivotally mounted to the rider and has a first section secured at opposite ends thereof to the rider. A bight portion of the first section serves as a lever. A second section of the bail is generally U-shaped and has opposite free ends thereof pivotally mounted to respective points along legs of the first section for facilitating engagement of a bight portion of the second section over an upturned edge of the strap. A mounting bracket is perpendicularly attached to the strap.
摘要翻译：用于圆柱形物体的夹具适于安装到表面并且包括具有用于环绕圆柱形物体的金属带的软管夹。该带包括多个间隔开的开口，用于与旋转地安装在骑手中的蜗轮构件啮合，该蜗轮构件可沿带的长度可滑动地定位。一个超中心的双重吊环枢转地安装在骑手上，并且具有固定在骑手的相对端的第一部分。第一部分的弯曲部分用作杠杆。吊钩的第二部分通常是U形的并且具有相对的自由端，其枢转地安装到沿着第一部分的腿部的相应位置，以便于将第二部分的弯曲部分接合在带子的向上的边缘上。安装支架垂直安装在吊带上。

7. 发明申请

US20070196843A1 Method for identification and monitoring of epigenetic modifications 审中-公开
标题翻译：鉴定和监测表观遗传修饰的方法
公开(公告)号：US20070196843A1
公开(公告)日：2007-08-23
申请号：US11638004
申请日：2006-12-13
申请人： Roland Green , Hans Bjornsson , Andrew Feinberg
发明人： Roland Green , Hans Bjornsson , Andrew Feinberg
IPC分类号： C12Q1/68 , G06F19/00
CPC分类号： C12Q1/6883 , C12Q1/6809 , C12Q1/6837 , C12Q2600/112 , C12Q2600/154 , C12Q2600/156 , C12Q2522/101 , C12Q2523/101 , C12Q2537/164 , C12Q2565/501
摘要： The present invention provides novel methods for identifying and monitoring epigenetic modifications, such as imprinted genes, using microarray based technology. Specifically, the invention detects imprinted genes by the presence of overlapping closed and open chromatin markers. The invention also discloses a method for detecting the loss of imprinting on a genome-wide scale, which is indicative of a variety of medical conditions. Diagnostic assays and chromatin structure markers for identifying gene imprinting and loss thereof are also disclosed.
摘要翻译：本发明提供用于鉴定和监测表观遗传修饰的新方法，例如使用基于微阵列技术的印迹基因。具体地，本发明通过重叠的闭合和开放的染色质标记物的存在来检测印迹基因。本发明还公开了一种在全基因组范围内检测印记损失的方法，其指示各种医学状况。还公开了用于鉴定基因印记及其损失的诊断测定和染色质结构标记。

8. 发明申请

US20120040343A1 Detecting and Sorting Methylated DNA Using a Synthetic Nanopore 有权
标题翻译：使用合成纳米孔检测和分选甲基化DNA
公开(公告)号：US20120040343A1
公开(公告)日：2012-02-16
申请号：US13133300
申请日：2009-12-18
申请人： Gregory Timp , Winston Timp , Andrew Feinberg , Utkur Mirsaidov
发明人： Gregory Timp , Winston Timp , Andrew Feinberg , Utkur Mirsaidov
IPC分类号： C12Q1/68 , G01N27/447 , B82Y15/00
CPC分类号： C12Q1/6827 , C12Q2565/631
摘要： Provided are methods for detecting, characterizing or separating DNA based on methylation. Heterogeneous DNA populations are separated based on DNA methylation by providing a membrane having a nanopore through which an electric field is applied. DNA of interest is introduced, and for a given threshold voltage across the nanopore, only DNA having a methylation parameter of interest may transit the pore, thereby facilitating detection, characterization, or separation of DNA based on methylation. The methods are optionally used to detect a disease state that is associated with DNA methylation including, but not limited to, cancer.
摘要翻译：提供了用于基于甲基化检测，表征或分离DNA的方法。通过提供具有通过其施加电场的纳米孔的膜，基于DNA甲基化分离不均匀DNA群体。引入目标DNA，对于纳米孔上的给定阈值电压，只有具有目标甲基化参数的DNA才能转移孔，从而便于基于甲基化的DNA的检测，表征或分离。任选地，所述方法用于检测与DNA甲基化相关的疾病状态，包括但不限于癌症。

9. 发明申请

US20060003359A1 Methods of screening for cell proliferation or neoplastic disorders 有权
公开(公告)号：US20060003359A1
公开(公告)日：2006-01-05
申请号：US11145331
申请日：2005-06-03
申请人： Andrew Feinberg , Christine Iacobuzio-Donahue , Dan Longo , Minoru Ko
发明人： Andrew Feinberg , Christine Iacobuzio-Donahue , Dan Longo , Minoru Ko
IPC分类号： C12Q1/68 , G01N33/574
CPC分类号： G01N33/574 , C12Q1/6886 , C12Q2600/154 , C12Q2600/156 , G01N33/5017 , G01N33/5023 , G01N33/57419 , G01N33/57434
摘要： The invention relates to methods and compositions for identifying subjects having, or predisposed to having, a neoplastic or cell proliferation or neoplastic disorder. The methods are applicable to any type of tissue sample and can be conducted on otherwise normal tissue.

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