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    • 2. 发明授权
    • Method for early diagnosis of alzheimer'S disease using phototransistor
    • 使用光电晶体管早期诊断阿尔茨海默氏病的方法
    • US09023607B2
    • 2015-05-05
    • US13093152
    • 2011-04-25
    • Kwan-Su KimCheol-Joo ChaeJae-Min KangJeong-Dae SuhMyung-Ae ChungKi-Bong Song
    • Kwan-Su KimCheol-Joo ChaeJae-Min KangJeong-Dae SuhMyung-Ae ChungKi-Bong Song
    • C12Q1/00B82Y15/00G01N33/68
    • B82Y15/00G01N33/6896G01N2800/2821
    • A method for diagnosing Alzheimer's disease by detecting the presence of beta-amyloid in cells using a phototransistor device. Cells that potentially contain beta-amyloid are provided and labeled with a multi-protein that selectively binds to beta-amyloid if present and that includes streptavidin, biotin and polyethylene glycol connected in sequence with the streptavidin being bound to a magnetic bead and the polyethylene glycol being bound to the beta-amyloid, to provide labeled cells. A phototransistor device including a channel layer is provided and a difference in photocurrent determined corresponding to incident light measured before and after selectively fixing the labeled cells on a surface of the channel layer by applying an external magnetic field effective to attract the magnetic bead to the phototransistor device through a permanent magnet positioned below the channel. Any difference between the first and second photocurrents indicates the presence of, and optionally amount of, labeled beta-amyloid in the cells.
    • 一种通过使用光电晶体管装置检测细胞中β-淀粉样蛋白的存在来诊断阿尔茨海默病的方法。 提供可能含有β-淀粉样蛋白的细胞并用选择性结合β-淀粉样蛋白(如果存在)的多蛋白标记,并且包括与链霉抗生物素蛋白结合到磁珠上的序列连接的链霉抗生物素蛋白,生物素和聚乙二醇,并且聚乙二醇 结合β-淀粉样蛋白,以提供标记细胞。 提供了包括沟道层的光电晶体管装置,并且通过施加有效吸引磁珠到光电晶体管的外部磁场选择性地将标记的单元选择性地固定在通道层的表面之前和之后测量的入射光确定的光电流差异 设备通过位于通道下方的永磁体。 第一和第二光电流之间的任何差异表明在细胞中存在和任选量的标记的β-淀粉样蛋白。