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1. 发明授权

US08129148B2 Process for preparation of chondroitin fraction 有权
$Process for preparation of chondroitin fraction$
标题翻译：软骨素部分的制备方法
公开(公告)号：US08129148B2
公开(公告)日：2012-03-06
申请号：US12064810
申请日：2006-08-23
申请人： Nobuo Sugiura , Koji Kimata
发明人： Nobuo Sugiura , Koji Kimata
IPC分类号： C12P19/28
CPC分类号： C12P19/26
摘要： Provided are a method for producing a fraction containing more than 50% of CH represented by the general formula (1), which comprises at least the step of allowing a glucuronic acid donor, an N-acetylgalactosamine donor, a saccharide receptor, a chondroitin polymerase derived from the Escherichia coli K4 strain, and Mn2+ at a final concentration of 0.02 to 100 mM to coexist, and performing a reaction thereof under conditions of 20 to 40° C. and pH 6 to 8 for 0.5 minutes to 4 hours, and a method for producing a fraction containing more than 50% of CH represented by the general formula (2), which comprises at least the step of performing the reaction under same conditions for 10 hours or longer, which enable industrial scale production of a CH fraction of a controlled even number saccharide and odd number saccharide content ratio by a simple procedure at a low cost. (GlcA-GalNAc)n (1) GalNAc-(GlcA-GalNAc)n (2) (In the formula, GlcA represents a glucuronic acid residue, GalNCAc represents a N-acetylgalactosamine residue, - represents a glycosidic bond, and n represents an arbitrary integer.)
摘要翻译：本发明提供了一种生产含有通式（1）所示的超过50％的CH的级分的方法，该方法至少包括使葡糖醛酸供体，N-乙酰半乳糖胺供体，糖受体，软骨素聚合酶来源于大肠杆菌K4菌株，最终浓度为0.02〜100mM的Mn2 +共存，在20〜40℃，pH6〜8的条件下进行0.5分钟〜4小时的反应，制备含有超过50％由通式（2）表示的CH的级分的方法，该方法至少包括在相同条件下进行反应10小时或更长时间的步骤，这使得工业规模生产CH部分通过简单的程序以低成本控制偶数糖和奇数糖含量比。（GlcA-GalNAc）n（1）GalNAc-（GlcA-GalNAc）n（2）（式中，GlcA表示葡萄糖醛酸残基，GaINCAc表示N-乙酰半乳糖胺残基， - 表示糖苷键，n表示任意整数）

2. 发明申请

US20110195476A1 MODIFIED CHONDROITIN SYNTHASE POLYPEPTIDE AND CRYSTAL THEREOF 有权
标题翻译：改性的合成酶聚合酶及其结晶
公开(公告)号：US20110195476A1
公开(公告)日：2011-08-11
申请号：US13046172
申请日：2011-03-11
申请人： Yoshimitsu KAKUTA , Takuo Osawa , Nobuo Sugiura , Koji Kimata
发明人： Yoshimitsu KAKUTA , Takuo Osawa , Nobuo Sugiura , Koji Kimata
IPC分类号： C12N9/00 , C12N1/00 , C12N15/63 , C07H21/04
CPC分类号： C12Y204/01175 , C12N9/1051 , C12P19/26
摘要： Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.
摘要翻译：公开了：（A）由SEQ ID NO：2的氨基酸序列组成的多肽，或（B）包含SEQ ID NO：2的氨基酸序列的多肽，包括缺失，取代或添加一个或几个氨基酸残留并具有软骨素合成酶活性; 编码该多肽的核酸; 一种生产多肽的方法，至少包括以下步骤：（1）表达所述核酸以产生所述多肽; 和（2）收集步骤（1）中产生的多肽; 和多肽的晶体。该晶体可以是单斜晶体或四方晶体。

3. 发明申请

US20090155851A1 NOVEL PROCESS FOR PREPARATION OF CHONDROITIN FRACTION 有权
标题翻译：制备新戊酰化酶的新方法
公开(公告)号：US20090155851A1
公开(公告)日：2009-06-18
申请号：US12064810
申请日：2006-08-23
申请人： Nobuo Sugiura , Koji Kimata
发明人： Nobuo Sugiura , Koji Kimata
IPC分类号： C12P19/26 , C08B37/00
CPC分类号： C12P19/26
摘要： Provided are a method for producing a fraction containing more than 50% of CH represented by the general formula (1), which comprises at least the step of allowing a glucuronic acid donor, an N-acetylgalactosamine donor, a saccharide receptor, a chondroitin polymerase derived from the Escherichia coli K4 strain, and Mn2+ at a final concentration of 0.02 to 100 mM to coexist, and performing a reaction thereof under conditions of 20 to 40° C. and pH 6 to 8 for 0.5 minutes to 4 hours, and a method for producing a fraction containing more than 50% of CH represented by the general formula (2), which comprises at least the step of performing the reaction under same conditions for 10 hours or longer, which enable industrial scale production of a CH fraction of a controlled even number saccharide and odd number saccharide content ratio by a simple procedure at a low cost. (GlcA-GalNAc)n (1) GalNAc-(GlcA-GalNAc)n (2) (In the formula, GlcA represents a glucuronic acid residue, GalNAc represents a N-acetylgalactosamine residue, - represents a glycosidic bond, and n represents an arbitrary integer.)
摘要翻译：提供了一种生产含有通式（1）所示的超过50％的CH的级分的方法，该方法至少包括使葡糖醛酸供体，N-乙酰半乳糖胺供体，糖受体，软骨素聚合酶来源于大肠杆菌K4菌株，最终浓度为0.02〜100mM的Mn2 +共存，在20〜40℃，pH6〜8的条件下进行0.5分钟〜4小时的反应，制备含有超过50％由通式（2）表示的CH的级分的方法，该方法至少包括在相同条件下进行反应10小时或更长时间的步骤，这使得工业规模生产CH部分通过简单的程序以低成本控制偶数糖和奇数糖含量比。 <？in-line-formula description =“In-line Formulas”end =“lead”？>（GlcA-GalNAc）n（1）<？in-line-formula description =“In-line Formulas”end =“tail “？”<？in-line-formula description =“In-line Formulas”end =“lead”？> GalNAc-（GlcA-GalNAc）n（2）<？in-line-formula description = （式中，GlcA表示葡萄糖醛酸残基，GaINAc表示N-乙酰半乳糖胺残基， - 表示糖苷键，n表示任意的整数。）

4. 发明申请

US20080070996A1 CHONDROITIN SYNTHASE AND NUCLEIC ACID ENCODING THE ENZYME 有权
标题翻译： CHONDROITIN SYNTHASE和核酸编码ENZYME
公开(公告)号：US20080070996A1
公开(公告)日：2008-03-20
申请号：US11748409
申请日：2007-05-14
申请人： Hisashi Narimatsu , Koji Kimata , Toshikazu Yada , Takashi Sato , Masanori Goto
发明人： Hisashi Narimatsu , Koji Kimata , Toshikazu Yada , Takashi Sato , Masanori Goto
IPC分类号： A61K35/00 , A61P43/00 , C07H21/00 , C12N1/00 , C12N15/63 , C12P19/04
CPC分类号： C12N9/1048
摘要： A human-derived novel chondroitin synthase, which is an enzyme for synthesizing a fundamental backbone of chondroitin and has both glucuronic acid transferring activity and N-acetylgalactosamine transferring activity.
摘要翻译：一种人源化的新型软骨素合成酶，其是用于合成软骨素的基础骨架的酶，具有葡萄糖醛酸转移活性和N-乙酰半乳糖胺转移活性。

5. 发明授权

US07273729B2 Chondroitin synthase and DNA encoding the enzyme 有权
标题翻译：软骨素合成酶和编码酶的DNA
公开(公告)号：US07273729B2
公开(公告)日：2007-09-25
申请号：US10516099
申请日：2003-05-30
申请人： Hisashi Narimatsu , Koji Kimata , Toshikazu Yada , Takashi Sato , Masanori Goto
发明人： Hisashi Narimatsu , Koji Kimata , Toshikazu Yada , Takashi Sato , Masanori Goto
IPC分类号： C12N9/10 , C12N15/00 , C12N5/02 , C12P21/06 , C12P19/42 , C12P19/04 , C07H21/04
CPC分类号： C12N9/1048 , C12P19/26
摘要： A human-derived novel chondroitin synthase, which is an enzyme for synthesizing a fundamental backbone of chondroitin and has both glucuronic acid transferase activity and N-acetylgalactosamine transferase activity.
摘要翻译：人源性新型软骨素合成酶，其是合成软骨素基础骨架的酶，具有葡糖醛酸转移酶活性和N-乙酰半乳糖胺转移酶活性。

6. 发明申请

US20070160593A1 Sugar chain-cleaving agent 审中-公开
标题翻译：糖链切割剂
公开(公告)号：US20070160593A1
公开(公告)日：2007-07-12
申请号：US10569676
申请日：2004-08-25
申请人： Hisashi Narimatsu , Koji Kimata , Toshikazu Yada
发明人： Hisashi Narimatsu , Koji Kimata , Toshikazu Yada
IPC分类号： A61K38/47 , C07H21/04 , C08B37/00 , C12P19/28 , C12N9/24
CPC分类号： A61K38/47 , C12N9/2402 , C12Y302/01097
摘要： A sugar chain-cleaving agent, which comprises a protein having activity of degrading chondroitin and/or chondroitin sulfate; a medicament which comprises the sugar chain-cleaving agent as an active ingredient; a method for specifically cleaving a sugar chain, which comprises reacting the protein with chondroitin and/or chondroitin sulfate; and a method for specifically producing a sugar chain of which molecular weight is decreased, which reacting the protein with chondroitin and/or chondroitin sulfate.
摘要翻译：一种糖链切割剂，其包含具有降解软骨素和/或硫酸软骨素的活性的蛋白质; 包含糖链切割剂作为活性成分的药物; 一种用于特异性切割糖链的方法，其包括使所述蛋白质与软骨素和/或硫酸软骨素反应; 特异性地制造分子量降低的糖链，使蛋白与软骨素和/或硫酸软骨素反应的方法。

7. 发明授权

US6162908A Polypeptide of human-origin hyaluronate synthetase and DNA encoding the same 有权
标题翻译：人源性透明质酸合成多肽和编码相同的DNA
公开(公告)号：US6162908A
公开(公告)日：2000-12-19
申请号：US155768
申请日：1998-10-05
申请人： Naoki Itano , Koji Kimata
发明人： Naoki Itano , Koji Kimata
IPC分类号： C12N9/10 , C12N15/56 , C12N9/26 , C12N9/00
CPC分类号： C12N9/1051
摘要： A DNA encoding at least part of a hyaluronan synthase of human origin, particularly encoding the whole or a part of an amino acid sequence shown by SEQ ID NO: 4. A polypeptide of the hyaluronan synthase of human origin, which is encoded by the DNA, may have a substitution, deletion or insertion of one or more amino acid residues that does not substantially lower an activity of synthesizing hyaluronan. A polypeptide of the hyaluronan synthase of human origin or a part thereof encoded by the DNA is also provided.
摘要翻译： PCT No.PCT / JP97 / 01111 Sec。 371日期：1998年10月5日 102（e）日期1998年10月5日PCT 1997年3月31日PCT公布。公开号WO97 / 38113 日期1997年10月16日编码人源的透明质酸合成酶的至少一部分的DNA，特别是编码SEQ ID NO：4所示的全部或部分氨基酸序列。人源性透明质酸合酶的多肽由DNA编码，可能具有不显着降低合成透明质酸的活性的一个或多个氨基酸残基的取代，缺失或插入。还提供了人源的透明质酸合酶或其由DNA编码的部分的多肽。

8. 发明授权

US5849722A Oligosaccharide having affinity for fibroblast growth factor and process for producing same 失效
标题翻译：对成纤维细胞生长因子具有亲和力的寡糖及其制造方法
公开(公告)号：US5849722A
公开(公告)日：1998-12-15
申请号：US471174
申请日：1995-06-06
申请人： Hiroko Habuchi , Sakaru Suzuki , Koji Kimata
发明人： Hiroko Habuchi , Sakaru Suzuki , Koji Kimata
IPC分类号： C08B37/00 , C12P19/26 , A61K31/725 , C08B37/10
CPC分类号： A61K31/715 , C08B37/0078 , C12P19/26
摘要： An oligosaccharide having an affinity for fibroblast growth factor, which is composed of 8 to 18 monosaccharide residues, wherein a principal disaccharide unit comprising L-iduronic acid 2-sulfate and N-sulfo-D-glucosamine and a process for producing the oligosaccharide comprising digesting heparan sulfate.
摘要翻译：一种对8-18个单糖残基组成的成纤维细胞生长因子亲和力的寡糖，其中包含L-艾可糖醛酸2-硫酸盐和N-磺基-D-葡糖胺的主要二糖单元和包含消化的寡糖的方法硫酸乙酰肝素。

9. 发明授权

US5470578A Antirheumatic composition 失效
标题翻译：抗风湿组合物
公开(公告)号：US5470578A
公开(公告)日：1995-11-28
申请号：US98936
申请日：1993-07-29
申请人： Shigehisa Aoki , Shinichi Iwasaki , Nobuo Sugiura , Sakaru Suzuki , Koji Kimata
发明人： Shigehisa Aoki , Shinichi Iwasaki , Nobuo Sugiura , Sakaru Suzuki , Koji Kimata
IPC分类号： A61K31/715 , A61K9/48 , A61K31/661 , A61K31/726 , A61K31/728 , A61P29/00 , C07H5/06 , A61K37/22
CPC分类号： A61K9/0019 , A61K47/48 , C07H5/06
摘要： This invention provides an antirheumatic composition which comprises a lipid-bound glycosaminoglycan or a salt thereof as an active ingredient and a pharmaceutically acceptable carrier and has an effect to inhibit extension of pannus.
摘要翻译：本发明提供一种抗风湿组合物，其包含脂质结合的糖胺聚糖或其盐作为活性成分和药学上可接受的载体，并且具有抑制血管inhibit延伸的作用。

10. 发明授权

US08067204B2 Long-chain chondroitin sugar chain and method for producing the same and method for promoting synthesis of chondroitin 有权
标题翻译：长链软骨素糖链及其生产方法及促进软骨素合成的方法
公开(公告)号：US08067204B2
公开(公告)日：2011-11-29
申请号：US12097725
申请日：2006-12-14
申请人： Nobuo Sugiura , Satoshi Shimokata , Koji Kimata
发明人： Nobuo Sugiura , Satoshi Shimokata , Koji Kimata
IPC分类号： C12P19/00
CPC分类号： C12P19/26 , C08B37/0069
摘要： A method for producing a chondroitin sugar chain comprises reacting a glucuronic acid donor, an N-acetyl galactosamine donor, a sugar receptor and a bacterial cell enzyme which synthesizes chondroitin in the presence of a surfactant. The surfactant is selected from polyoxyethylene octadecyl amine, n-decanoyl-N-methylglucamide, sodium cholate, n-octyl-β-D-thioglucopyranoside, n-nonyl-β-D-thiomaltopyranoside, sucrose monocholate, sucrose monocaprate, and sucrose monolaurate. The chondroitin sugar chain has all the following properties: a weight average molecular weight: 50,000 or more when measured by gel filtration chromatography; it is completely degraded to disaccharides with chondroitinase ABC; and when the sugar chain is decomposed with chondroitinase ABC and the decomposed products are subjected to a disaccharide analysis, substantially all of them correspond to an unsaturated disaccharide unit of chondroitin.
摘要翻译：生产软骨素糖链的方法包括使葡糖醛酸供体，N-乙酰半乳糖胺供体，糖受体和在表面活性剂存在下合成软骨素的细菌细胞酶的反应。表面活性剂选自聚氧乙烯十八烷基胺，正癸酰基-N-甲基葡萄糖酰胺，胆酸钠，正辛基-β-硫代吡喃葡萄糖苷，正壬基-β-硫代木糖淀粉，蔗糖单胆酸酯，蔗糖单癸酸酯和蔗糖单月桂酸酯。软骨素糖链具有以下性质：通过凝胶过滤色谱法测定的重均分子量为50,000以上; 软骨素酶ABC完全降解成二糖; 并且当糖链用软骨素酶ABC分解并且分解产物进行二糖分析时，其基本上都对应于软骨素的不饱和二糖单元。

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