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1. 发明申请

US20070045126A1 Oxidizable species as an internal reference for biosensors and method of use 有权
标题翻译：可氧化物种作为生物传感器的内部参考和使用方法
公开(公告)号：US20070045126A1
公开(公告)日：2007-03-01
申请号：US10590765
申请日：2005-02-04
申请人： Greg Beer , Huan-Ping Wu , Kin-Fai Yip
发明人： Greg Beer , Huan-Ping Wu , Kin-Fai Yip
IPC分类号： C12Q1/00 , G01N33/487
CPC分类号： G01N27/3273 , C12Q1/004 , C12Q1/006 , G01N27/3271 , G01N27/3272 , G01N27/3274 , G01N33/54373
摘要： A biosensor (102) for determining the presence or amount of a substance in a sample and methods of use of the biosensor (102) are provided. The biosensor (102) for receiving a user sample to be analyzed includes a mixture for electrochemical reaction with an analyte. The mixture includes an enzyme, a mediator and an oxidizable species as an internal reference.
摘要翻译：提供了一种用于确定样品中物质的存在或量以及生物传感器（102）的使用方法的生物传感器（102）。用于接收待分析的用户样品的生物传感器（102）包括用于与分析物的电化学反应的混合物。该混合物包括酶，介体和可氧化物质作为内部参考。

2. 发明授权

US5653863A Method for reducing bias in amperometric sensors 失效
标题翻译：降低电流传感器偏压的方法
公开(公告)号：US5653863A
公开(公告)日：1997-08-05
申请号：US647219
申请日：1996-05-09
申请人： Marvin A. Genshaw , Dijia Huang , Matthew K. Musho , Kin Fai Yip
发明人： Marvin A. Genshaw , Dijia Huang , Matthew K. Musho , Kin Fai Yip
IPC分类号： G01N33/483 , C12Q1/00 , G01N27/327 , G01N27/416 , G01N27/48 , G01N33/66 , G01N33/72 , G01N33/92 , G01N27/26
CPC分类号： C12Q1/004
摘要： Apparatus and method are provided for determining the concentration of an analyte in a fluid test sample by applying the fluid test sample to the surface of a working electrode which is electrochemically connected to a reference electrode which surface bears a composition comprising an enzyme specific for the analyte. A mediator is reduced in response to a reaction between the analyte and the enzyme. An oxidizing potential is applied between the electrodes to return at least a portion of the mediator back to its oxidized form before determining the concentration of the analyte to thereby increase the accuracy of the analyte determination. Following this initially applied potential, the circuit is switched to an open circuit or to a potential that substantially reduces the current to minimize the rate of electrochemical potential at the working electrode. A second potential is applied between the electrodes and the current generated in the fluid test sample is measured to determine analyte concentration. Optionally, the accuracy of the analyte determination is further enhanced algorithmically.
摘要翻译：提供了设备和方法，用于通过将流体测试样品施加到电化学连接到参考电极的工作电极的表面上来确定流体测试样品中分析物的浓度，所述参考电极的表面承载包含对于分析物特异的酶的组合物。响应分析物和酶之间的反应而减少介体。在确定分析物的浓度之前，在电极之间施加氧化电位以将至少一部分介体返回到其氧化形式，从而提高分析物确定的准确度。在该最初施加的电位之后，电路被切换到开路或电位，其基本上减小电流以最小化工作电极处的电化学电势的速率。在电极之间施加第二电位，并测量在流体测试样品中产生的电流以确定分析物浓度。任选地，分析物确定的准确度进一步在算法上增强。

3. 发明授权

US08696880B2 Oxidizable species as an internal reference for biosensors and method of use 有权
标题翻译：可氧化物种作为生物传感器的内部参考和使用方法
公开(公告)号：US08696880B2
公开(公告)日：2014-04-15
申请号：US10590765
申请日：2005-02-04
申请人： Greg P. Beer , Huan-Ping Wu , Kin-Fai Yip
发明人： Greg P. Beer , Huan-Ping Wu , Kin-Fai Yip
IPC分类号： G01N27/327
CPC分类号： G01N27/3273 , C12Q1/004 , C12Q1/006 , G01N27/3271 , G01N27/3272 , G01N27/3274 , G01N33/54373
摘要： A biosensor (102) for determining the presence or amount of a substance in a sample and methods of use of the biosensor (102) are provided. The biosensor (102) for receiving a user sample to be analyzed includes a mixture for electrochemical reaction with an analyte. The mixture includes an enzyme, a mediator and an oxidizable species as an internal reference.
摘要翻译：提供了一种用于确定样品中物质的存在或量以及生物传感器（102）的使用方法的生物传感器（102）。用于接收待分析的用户样品的生物传感器（102）包括用于与分析物的电化学反应的混合物。该混合物包括酶，介体和可氧化物质作为内部参考。

4. 发明授权

US6043043A Method for the determination of hemoglobin adducts 失效
标题翻译：血红蛋白加合物测定方法
公开(公告)号：US6043043A
公开(公告)日：2000-03-28
申请号：US77546
申请日：1993-06-18
申请人： Kin-Fai Yip
发明人： Kin-Fai Yip
IPC分类号： G01N33/53 , G01N33/72 , G01N15/06 , G01N31/00
CPC分类号： G01N33/723 , Y10T436/105831
摘要： Disclosed is an improvement to the method of determining the concentration of a hemoglobin adduct in a blood sample by the steps of assaying the blood sample for the total amount of hemoglobin, assaying the blood sample for the hemoglobin adduct, and dividing the hemoglobin adduct concentration by the total hemoglobin concentration. The improvement involves normalizing the measurement of the hemoglobin adduct to the total amount of hemoglobin in the blood sample.
摘要翻译：公开了通过以下步骤确定血液样品中血红蛋白加合物的浓度的方法的改进：通过测定血液样品中血红蛋白总量，测定血液样品中血红蛋白加合物，并将血红蛋白加合物浓度除以总血红蛋白浓度。该改进涉及将血红蛋白加合物的测量标准化为血液样品中血红蛋白的总量。

5. 发明授权

US5133934A Ionosphere and reporter substance incorporated into a hydrophobic carrier 失效
标题翻译：电离层和记录物质掺入疏水载体中
公开(公告)号：US5133934A
公开(公告)日：1992-07-28
申请号：US659234
申请日：1991-02-22
申请人： James B. Denton , Kin-Fai Yip
发明人： James B. Denton , Kin-Fai Yip
IPC分类号： C07D323/00 , G01N33/52 , G01N33/84
CPC分类号： G01N33/84 , C07D323/00 , G01N33/521
摘要： Compositions and test articles comprisng ionophore of an optically active isomer of bis(12-crown-4-methyl)dialkyl malonate, wherein alkyl group is methyl and the second alkyl group has 4 to 20 carbon atoms, with a reporter substance incorporated in a hydrophobic carrier are disclosed.
摘要翻译：包含双（12-冠-4-甲基）二烷基丙二酸酯的光学活性异构体的离子载体的组合物和测试制品，其中烷基是甲基，第二个烷基具有4至20个碳原子，报道物质掺入疏水性载体被公开。

6. 发明授权

US5162237A Reaction cassette for preforming sequential analytical assays by noncentrifugal and noncapillary manipulations 失效
标题翻译：用于通过非离心和非毛细管理进行顺序分析测定的反应盒
公开(公告)号：US5162237A
公开(公告)日：1992-11-10
申请号：US774362
申请日：1991-10-08
申请人： Lowry J. Messenger , Christine D. Nelson , Kin-Fai Yip , Frank W. Wogoman
发明人： Lowry J. Messenger , Christine D. Nelson , Kin-Fai Yip , Frank W. Wogoman
IPC分类号： B01L3/00 , G01N21/07 , G01N33/53 , G01N33/543 , G01N33/72
CPC分类号： B01L3/502 , G01N21/07 , G01N33/5302 , G01N33/5304 , G01N33/54366 , G01N33/723 , G01N33/726 , Y10S436/808
摘要： Analytical reaction cassette and method for performing sequential analytical assay procedures to determine the amount of an analyte in a liquid test mixture. The reaction cassette can be in the form of a substantially square container having a substantially horizontal axis of rotation and incorporated with one or more analytical reagents such that they are contacted with a liquid test mixture in a desired ordered sequence to perform a particular assay procedure. Corners provided by the substantially square configuration of the reaction cassette disrupt the flow of liquids disposed in the reaction cassette upon contact therewith to thereby agitate and mix such liquids. A liquid disposed in the reaction cassette is capable of being manipulated and mixed therein by rotating the reaction cassette about the horizontal axis at sufficiently low velocities wherein the transport of such liquid is noncentrifugal and due substantially only to gravitational force. The reaction cassette is particularly useful for performing immunoturbidimetric assays, e.g., for the determination of glycated hemoglobin.
摘要翻译：分析反应盒和用于执行顺序分析测定程序以确定液体测试混合物中分析物的量的方法。反应盒可以是具有基本上水平的旋转轴线的基本上为方形的容器的形式，并结合有一种或多种分析试剂，使得它们以所需的有序序列与液体测试混合物接触以进行特定的测定程序。通过反应盒的大致正方形配置提供的角部在与反应盒接触时破坏设置在反应盒中的液体的流动，从而搅拌并混合这些液体。设置在反应盒中的液体能够通过以足够低的速度旋转反应盒围绕水平轴线而被操纵和混合，其中这种液体的输送是非离心的，并且基本上仅仅由重力引起。反应盒特别可用于进行免疫比浊测定，例如用于测定糖化血红蛋白。

7. 发明授权

US06391645B1 Method and apparatus for correcting ambient temperature effect in biosensors 失效
标题翻译：用于校正生物传感器环境温度影响的方法和装置
公开(公告)号：US06391645B1
公开(公告)日：2002-05-21
申请号：US08854440
申请日：1997-05-12
申请人： Dijia Huang , Brenda L. Tudor , Kin-Fai Yip
发明人： Dijia Huang , Brenda L. Tudor , Kin-Fai Yip
IPC分类号： G01N2712
CPC分类号： C12Q1/006 , G01N27/3274 , Y10T436/144444
摘要： A method and apparatus are provided for correcting ambient temperature effect in biosensors. An ambient temperature value is measured. A sample is applied to the biosensors, then a current generated in the test sample is measured. An observed analyte concentration value is calculated from the current through a standard response curve. The observed analyte concentration is then modified utilizing the measured ambient temperature value to thereby increase the accuracy of the analyte determination. The analyte concentration value can be calculated by solving the following equation: G 2 = G 1 - ( T 2 2 - 24 2 ) * I2 - ( T 2 - 24 ) * I1 ( T 2 2 - 24 2 ) * S2 + ( T 2 - 24 ) * S1 + 1 where G1 is said observed analyte concentration value, T2 is said measured ambient temperature value and I1, I2, S1, and S2 are predetermined parameters.
摘要翻译：提供了一种用于校正生物传感器中的环境温度效应的方法和装置。测量环境温度值。将样品应用于生物传感器，然后测量在测试样品中产生的电流。从通过标准响应曲线的电流计算观察到的分析物浓度值。然后使用测量的环境温度值改变观察到的分析物浓度，从而提高分析物测定的准确度。分析物浓度值可以通过求解以下公式计算：其中G1是所述观察到的分析物浓度值，T2是所述测量的环境温度值，I1，I2，S1和S2是预定参数。

8. 发明授权

US5464777A Dry reagent for creatinine assay 失效
标题翻译：用于肌酐测定的干试剂
公开(公告)号：US5464777A
公开(公告)日：1995-11-07
申请号：US312559
申请日：1994-09-26
申请人： Kin-Fai Yip
发明人： Kin-Fai Yip
IPC分类号： G01N33/493 , G01N33/52 , G01N33/53 , G01N33/531 , G01N33/536 , G01N33/70 , G01N33/546 , G01N33/557
CPC分类号： G01N33/70 , Y10S435/973 , Y10T436/147777
摘要： A method for the determination of protein and creatinine in urine. An organic acid, in the form of its lithium salt, is dried onto a suitable substrate in the second reaction zone of a reaction vessel having a first reaction zone for the immunometric determination of the protein and a second reaction zone for the colorimetric determination of creatinine. After completion of the immunometric determination of the protein the dried organic salt is contacted with the urine containing creatinine resulting in solubilization of the organic acid salt as creatinine reactive reagent thereby facilitating the colorimetric determination of the creatinine.
摘要翻译：一种测定尿液中蛋白质和肌酐的方法。以其锂盐形式的有机酸在具有用于免疫测定蛋白质的第一反应区域的反应容器的第二反应区中被干燥到合适的底物上，以及用于比例测定肌酸酐的第二反应区。完成蛋白质的免疫测定后，将干燥的有机盐与含有肌酸酐的尿液接触，导致有机酸盐作为肌酸酐反应试剂的溶解，从而便于肌酸酐的比色测定。

9. 发明授权

US5047563A Chiral crown ethers 失效
标题翻译：手性冠醚
公开(公告)号：US5047563A
公开(公告)日：1991-09-10
申请号：US95012
申请日：1987-09-10
申请人： James B. Denton , Kin-Fai Yip
发明人： James B. Denton , Kin-Fai Yip
IPC分类号： C07D323/00 , G01N21/78 , G01N27/333 , G01N33/52 , G01N33/84
CPC分类号： C07D323/00 , G01N33/521 , G01N33/84
摘要： The synthesis and use of optically active isomers of bis(12-crown-4-methyl) dialkylmalonates as sodium specific ionophores is described. The synthesis of optically active isomers of hydroxymethyl 12-crown-4 useful in synthesizing ionophores is also described.

10. 发明授权

US4898824A Crosslinked polyacrylamide-sulfhydryl polymer for immobilization of biologically active substances 失效
标题翻译：用于固定生物活性物质的交联聚丙烯酰胺 - 巯基聚合物
公开(公告)号：US4898824A
公开(公告)日：1990-02-06
申请号：US939904
申请日：1986-12-09
申请人： Kin-Fai Yip
发明人： Kin-Fai Yip
IPC分类号： C08F20/56 , C08F20/00 , C08F220/56 , C12N11/08 , G01N33/545
CPC分类号： G01N33/545 , C08F220/56 , C12N11/08
摘要： A water-insoluble, crosslinked polyacrylamide copolymer is prepared having recurring monomeric residues of acrylamide, bisacrylamide or analogs thereof, and N,N'-bisacrylylcystamine or analogs thereof. The crosslinked copolymer can be formed into discrete particles having crosslinking disulfide groups on their external surface which can be reduced to provide activated particles having exposed chemically active sulfhydryl groups. The activated polyacrylamide particles are free of residual amino and carboxylic acid groups and can be reacted with suitable haptens and other biologically active substances to provide insolubilized forms thereof.
摘要翻译：制备具有丙烯酰胺，双丙烯酰胺或其类似物的重复单体残基和N，N'-双丙烯基胱胺或其类似物的水不溶性交联的聚丙烯酰胺共聚物。交联共聚物可以在其外表面上形成具有交联二硫键的离散颗粒，其可被还原以提供具有暴露的化学活性巯基的活化颗粒。活化的聚丙烯酰胺颗粒没有残留的氨基和羧酸基团，并且可以与合适的半抗原和其他生物活性物质反应以提供其不溶性形式。

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