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1. 发明申请

US20120301466A1 CHEMICALLY PROGRAMMABLE IMMUNITY 审中-公开
标题翻译：化学可编程免疫
公开(公告)号：US20120301466A1
公开(公告)日：2012-11-29
申请号：US13567930
申请日：2012-08-06
申请人： Kary B. MULLIS
发明人： Kary B. MULLIS
IPC分类号： A61K39/395 , A61K35/74 , A61P31/00 , A61K35/76
CPC分类号： A61K39/07 , A61K39/385 , A61K2039/505 , A61K2039/541 , A61K2039/6025 , A61K2039/627 , C07K16/1242 , C07K2317/55 , Y02A50/386
摘要： Methods and compositions for immediately immunizing an individual against any molecule or compound. The present invention comprises an immunity linker with at least two sites; (1) at least one first binding site that binds to an immune response component in an individual that has been pre-immunized with a universal immunogen, and (2) at least one second binding site that binds specifically to a desired compound or molecule, the target.
摘要翻译：立即将个体免疫任何分子或化合物的方法和组合物。本发明包括具有至少两个位点的免疫接头; （1）至少一个第一结合位点，其结合已经用通用免疫原预先免疫的个体中的免疫应答组分，和（2）至少一个特异性结合所需化合物或分子的第二结合位点，目标。

2. 发明申请

US20100285052A1 Chemically Programmable Immunity 有权
标题翻译：化学可编程抗扰度
公开(公告)号：US20100285052A1
公开(公告)日：2010-11-11
申请号：US12774194
申请日：2010-05-05
申请人： KARY B. MULLIS , JEEVALATHA VIVEKANANDA , JOHNATHAN LLOYD KIEL , RONALD M. COOK
发明人： KARY B. MULLIS , JEEVALATHA VIVEKANANDA , JOHNATHAN LLOYD KIEL , RONALD M. COOK
IPC分类号： A61K39/385 , C07H21/00 , C07K16/18 , A61K35/00 , A61K31/7052 , A61P37/04 , C07K14/00 , A61K39/02 , A61K39/05 , A61K39/07 , A61K39/08 , A61K39/106 , A61K39/108 , A61K39/12 , A61K39/00
CPC分类号： A61K31/7088 , Y02A50/471 , Y02A50/473
摘要： Methods and compositions for immediately immunizing an individual against any molecule or compound are provided. The present invention is directed to an immunity linker with at least two sites; (1) at least one first binding site that binds to an immune response component in an individual, and (2) at least one second binding site that binds specifically to a desired compound or molecule, the target. The second binding sites are preferably thiolated aptamers that have the benefit of increased stability, resistance to degradation and longer circulating half life. Methods of making and using pharmaceutical compositions including immunity linker molecules having a thiolated aptamer are also provided.
摘要翻译：提供了立即将个体免受任何分子或化合物的方法和组合物。本发明涉及具有至少两个位点的免疫接头; （1）结合个体中的免疫应答组分的至少一个第一结合位点，和（2）至少一个第二结合位点，其特异性结合所需化合物或分子，靶标。第二结合位点优选为具有增加的稳定性，耐降解性和更长的循环半衰期益处的硫醇化适体。还提供了制备和使用药物组合物的方法，包括具有巯基适体的免疫接头分子。

3. 发明授权

US5468613A Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids 失效
标题翻译：用于检测核酸中存在的特异性核苷酸变异和遗传多态性的方法
公开(公告)号：US5468613A
公开(公告)日：1995-11-21
申请号：US491210
申请日：1990-03-09
申请人： Henry A. Erlich , Glenn Horn , Randall K. Saiki , Kary B. Mullis
发明人： Henry A. Erlich , Glenn Horn , Randall K. Saiki , Kary B. Mullis
IPC分类号： C12N15/09 , C07K14/74 , C12Q1/68 , G01N33/50 , C12N9/12 , C12N15/11 , C12P19/34
CPC分类号： C12Q1/6837 , C07K14/70539 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6881 , C12Q1/6883 , C12Q2600/156
摘要： Single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process whereby the sample suspected of containing the relevant nucleic acid is repeatedly treated with primers, nucleotide triphosphates, and an agent for polymerization of the triphosphates and then denatured, in a process which amplifies the sequence containing the nucleotide variation if it is present. In one embodiment, the sample is spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe. Hybridization of the probe to the sample is detected by the label on the probe.
摘要翻译：核酸序列中的单个或多个核苷酸变异可通过以下过程在核酸中检测，其中怀疑含有相关核酸的样品用引物，三磷酸核苷酸和用于聚合三磷酸酯的试剂重复处理，然后变性如果存在，则扩增含有核苷酸变异的序列的过程。在一个实施方案中，将样品点在膜上并用标记的序列特异性寡核苷酸探针处理。通过探头上的标签检测探针与样品的杂交。

4. 发明授权

US5187083A Rapid purification of DNA 失效
标题翻译：快速纯化DNA
公开(公告)号：US5187083A
公开(公告)日：1993-02-16
申请号：US611921
申请日：1990-11-13
申请人： Kary B. Mullis
发明人： Kary B. Mullis
IPC分类号： C12N1/06 , C12N15/10
CPC分类号： C12N1/06 , C12N15/1017 , Y10S435/803 , Y10S435/82
摘要： The present invention provides a method for rapidly obtaining substantially pure DNA from a biological sample containing cells. The method involves gently lysing the membranes of the cells to yield a lysate containing genomic DNA in a high molecular weight form. The lysate is moved through a porous filter to selectively trap the high molecular weight DNA on the filter. The DNA is released from the filter using an aqueous solution to form a solution containing substantially purified DNA, from which the DNA is recovered.
摘要翻译：本发明提供从含有细胞的生物样品中快速获得基本上纯的DNA的方法。该方法包括轻轻地裂解细胞的膜以产生含有高分子量形式的基因组DNA的裂解物。将裂解物移动通过多孔过滤器以选择性地将高分子量DNA捕获在过滤器上。使用水溶液从过滤器中释放DNA以形成含有基本上纯化的DNA的溶液，从中回收DNA。

5. 发明授权

US4965188A Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme 失效
标题翻译：使用热稳定酶扩增，检测和/或克隆核酸序列的方法
公开(公告)号：US4965188A
公开(公告)日：1990-10-23
申请号：US63647
申请日：1987-06-17
申请人： Kary B. Mullis , Henry A. Erlich , David H. Gelfand , Glenn Horn , Randall K. Saiki
发明人： Kary B. Mullis , Henry A. Erlich , David H. Gelfand , Glenn Horn , Randall K. Saiki
IPC分类号： C12N9/12 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C12P19/34 , C12N9/1252 , C12Q1/6844
摘要： A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The amplified sequence can be readily detected. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
摘要翻译：用于扩增核酸或其混合物中包含的任何靶核酸序列的方法包括用摩尔过量的两个寡核苷酸引物处理所述核酸的分开的互补链，并用热稳定酶扩增引物以形成互补引物延伸产物，其起作用作为合成所需核酸序列的模板。可以容易地检测扩增的序列。反应的步骤可以根据需要经常重复，并且涉及温度循环以进行杂交，促进酶的活性和所形成的杂交体的变性。

6. 发明授权

US4683202A Process for amplifying nucleic acid sequences 失效
标题翻译：扩增核酸序列的方法
公开(公告)号：US4683202A
公开(公告)日：1987-07-28
申请号：US791308
申请日：1985-10-25
申请人： Kary B. Mullis
发明人： Kary B. Mullis
IPC分类号： C12N15/09 , B01L7/00 , C07H21/00 , C07K14/805 , C12N15/00 , C12N15/10 , C12P19/34 , C12Q1/68 , C40B40/06 , C40B60/14 , C07H21/02 , C07H21/04 , C12N1/00
CPC分类号： C12N15/10 , B01L7/52 , C07K14/805 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , B01J2219/00495 , B01J2219/0059 , B01J2219/00722 , C12Q1/6883 , C12Q1/6886 , C12Q1/689 , C40B40/06 , C40B60/14
摘要： The present invention is directed to a process for amplifying any desired specific nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, and extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.

7. 发明授权

US07850975B2 Chemically programmable immunity 有权
标题翻译：化学可编程免疫
公开(公告)号：US07850975B2
公开(公告)日：2010-12-14
申请号：US11606564
申请日：2006-11-30
申请人： Kary B. Mullis
发明人： Kary B. Mullis
IPC分类号： A61K39/00 , A61K31/7088 , C07H21/00
CPC分类号： A61K39/385 , A61K2039/6025 , Y02A50/386
摘要： The present invention is related to methods and compositions that are capable of immediately immunizing a human or animal against any molecule or compound. The present invention comprises an immunity linker molecule with at least two sites; (1) a first binding site that binds to an immune system molecule in a human or animal that has been preimmunized against the first binding site, and (2) one or more second binding sites that bind specifically to a desired compound or molecule. The first binding site and the second binding site(s) are linked by a linker portion of the molecule.
摘要翻译：本发明涉及能够立即针对任何分子或化合物免疫人或动物的方法和组合物。本发明包括具有至少两个位点的免疫接头分子; （1）结合已针对第一结合位点预免疫的人或动物中的免疫系统分子的第一结合位点，和（2）特异性结合所需化合物或分子的一个或多个第二结合位点。第一结合位点和第二结合位点通过分子的接头部分连接。

8. 发明授权

US5386022A Primes and probes for the amplification and detection of aids associated nucleic acids 失效
标题翻译：引物和探针，用于扩增和检测辅助相关核酸
公开(公告)号：US5386022A
公开(公告)日：1995-01-31
申请号：US92767
申请日：1993-07-16
申请人： John J. Sninsky , Shirley Y. Kwok , David Mack , Henry A. Erlich , Kary B. Mullis
发明人： John J. Sninsky , Shirley Y. Kwok , David Mack , Henry A. Erlich , Kary B. Mullis
IPC分类号： B01L7/00 , C07K14/805 , C12N15/10 , C12Q1/68 , C12Q1/70 , C40B40/06 , C40B60/14 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/706 , B01L7/52 , C07K14/805 , C12N15/10 , C12Q1/6823 , C12Q1/6853 , C12Q1/686 , C12Q1/6865 , C12Q1/6876 , C12Q1/6881 , C12Q1/70 , C12Q1/703 , B01J2219/00495 , B01J2219/0059 , B01J2219/00722 , C40B40/06 , C40B60/14
摘要： The presence or absence of a nucleic acid sequence associated with AIDS in a sample containing one or more nucleic acids and suspected of containing such sequence can be detected by amplifying the sequence using primers to form extension products as templates and detecting the amplified product if it is present. This may be accomplished by adding a labeled hybridization probe to the amplified product either free in solution or after immobilization on a solid support. Exemplary primers and probes for amplifying and detecting AIDS virus are provided.
摘要翻译：含有一个或多个核酸并且怀疑含有此类序列的样品中与AIDS相关的核酸序列的存在或不存在可以通过使用引物扩增序列来检测以形成扩增产物作为模板并检测扩增产物当下。这可以通过将标记的杂交探针加入到在溶液中游离或固定在固体支持物上的扩增产物来实现。提供了用于扩增和检测艾滋病病毒的示例性引物和探针。

9. 发明授权

US5333675A Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps 失效
标题翻译：用于使用加热和冷却步骤进行核酸序列的自动扩增和测定的装置和方法
公开(公告)号：US5333675A
公开(公告)日：1994-08-02
申请号：US21624
申请日：1993-02-22
申请人： Kary B. Mullis , Larry Johnson , Richard A. Leath , Timothy J. Wennberg , Louis M. Mezei , Joseph T. Widunas
发明人： Kary B. Mullis , Larry Johnson , Richard A. Leath , Timothy J. Wennberg , Louis M. Mezei , Joseph T. Widunas
IPC分类号： B01J19/00 , B01L7/00 , C07H21/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N35/02 , G05B17/00
CPC分类号： B01L7/52 , B01J19/0046 , C07H21/00 , B01J2219/00495 , B01J2219/0059 , B01J2219/00689 , B01J2219/00722 , C40B40/06 , C40B60/14 , Y10T436/115831
摘要： There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.
摘要翻译：本文公开了一种用于在计算机控制下进行核酸扩增的机器。该机器在主机的控制下使用多个加热和冷却系统中的任何一个，其引导加热和冷却系统在该过程中适当的时间对含反应室的热交换器进行加热和冷却。反应室预先加载待扩增的核酸，催化聚合的热稳定酶，特异性寡核苷酸引物和四种不同的核苷酸三磷酸。还公开了由机器实现的扩增链反应的方法，其利用热稳定性酶。

10. 发明授权

US4754065A Precursor to nucleic acid probe 失效
标题翻译：核酸探针前体
公开(公告)号：US4754065A
公开(公告)日：1988-06-28
申请号：US72536
申请日：1987-07-13
申请人： Corey H. Levenson , Kary B. Mullis
发明人： Corey H. Levenson , Kary B. Mullis
IPC分类号： C07D493/04 , C07D513/04 , C07D519/00 , C07H21/00 , C07C103/44
CPC分类号： C07D493/04 , C07D513/04 , C07H21/00
摘要： Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling reagent is of the formula:[A] [B] Lwhere A is an alkylating intercalation moiety, B is a divalent organic moiety of the formula: ##STR1## where Y is O, NH or N--CHO, x is a number from 1 to 4, y is a number from 2 to 4, and L is a monovalent label moiety, wherein B is exclusive of any portion of the intercalation and label moieties.Preferably A is a 4-methylene-substituted psoralen moiety, and most preferably A is a 4'-methylene-substituted-4,5',8-trimethylpsoralen moiety and L is biotin.
摘要翻译：可以通过将标记试剂的烷基化插层部分嵌入部分双链核酸中以形成复合物并激活复合物以引起试剂和核酸之间的共价键来标记核酸。优选地，标记的核酸是用于检测能够与核酸的杂交区域杂交的核酸序列的杂交探针。还优选地，标记部分是非放射性的。标记试剂具有下式：[A] [B] L，其中A是烷基化插层部分，B是下式的二价有机部分：其中Y是O，NH或N-CHO，x是 1至4的数，y为2至4的数，L为单价标记部分，其中B不包括插入和标记部分的任何部分。优选A是4-亚甲基取代的补骨脂素部分，最优选A是4'-亚甲基取代的-4,5'，8-三甲基肉豆蔻酸酯部分，L是生物素。

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