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1. 发明申请

US20070298422A1 Procedure for the analysis of a sample 审中-公开
标题翻译：样品分析程序
公开(公告)号：US20070298422A1
公开(公告)日：2007-12-27
申请号：US11656463
申请日：2007-01-23
申请人： Jorn Mosner , Sebastian Schmidt
发明人： Jorn Mosner , Sebastian Schmidt
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6872 , C12Q2563/167 , C12Q2545/101 , C12Q2521/301
摘要： A procedure for the analysis of a sample is disclosed, where the sample includes at least one nucleic acid molecule having at least one nucleic acid sequence. In at least one embodiment, the procedure includes making available a comparison molecule of known concentration having a known comparison sequence, where the comparison sequence has a defined mass difference in comparison to a reference sequence of the nucleic acid sequence; cleaving the nucleic acid sequence to give fragments of the nucleic acid sequence and the comparison sequence to give fragments of the comparison sequence, determining a mass spectrum of a mixture of the fragments of the nucleic acid sequence and of the comparison sequence by means of mass spectrometry, determining a comparison spectrum of the fragments of the comparison sequence by means of mass spectrometry, determination of the concentration of the nucleic acid sequence from the mass spectrum and the comparison spectrum and determining still unknown sequence variations of the nucleic acid sequence in comparison to the reference sequence from the mass spectrum and a reference spectrum of the reference sequence.
摘要翻译：公开了用于分析样品的方法，其中样品包括至少一个具有至少一个核酸序列的核酸分子。在至少一个实施方案中，该方法包括提供具有已知比较序列的已知浓度的比较分子，其中比较序列与核酸序列的参考序列相比具有确定的质量差异; 切割核酸序列以得到核酸序列和比较序列的片段，得到比较序列的片段，通过质谱法测定核酸序列片段和比较序列的混合物的质谱图，通过质谱法测定比较序列的片段的比较谱，从质谱确定核酸序列的浓度和比较光谱，并确定核酸序列的未知序列变异与来自质谱的参考序列和参考序列的参考光谱。

2. 发明申请

US20070298429A1 Process for determining the concentration of nucleic acids 有权
标题翻译：确定核酸浓度的方法
公开(公告)号：US20070298429A1
公开(公告)日：2007-12-27
申请号：US11808835
申请日：2007-06-13
申请人： Walter Gumbrecht , Jorn Mosner , Sebastian Schmidt
发明人： Walter Gumbrecht , Jorn Mosner , Sebastian Schmidt
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： C12Q1/686 , B01L3/5027 , B01L7/52 , B01L2200/143 , B01L2200/16 , B01L2300/0645 , B01L2300/0816 , B01L2300/0867 , B01L2300/1822 , B01L2400/0487 , B01L2400/0633 , C12Q1/6851 , C12Q2565/629
摘要： A process is disclosed for determining the concentration of nucleic acids in a sample in a microfluidic device. In at least one embodiment, the method includes a) introducing the sample into a first chamber, b) carrying out a number of cycles of an amplification reaction to be carried out in cycles for amplifying nucleic acids, c) transferring a defined volume which is a fraction of the volume of the first chamber and which has amplified nucleic acids into a second chamber and replacing the transferred defined volume with fresh reagents for the amplification reaction, d) determining the concentration of the amplified nucleic acids in a second chamber equipped with an element to determine concentrations, and e) repeating steps b)-d) until a concentration of the amplified nucleic acids which is within a range is determined in the second chamber. An arrangement is further disclosed.
摘要翻译：公开了用于确定微流体装置中样品中核酸浓度的方法。在至少一个实施方案中，所述方法包括：a）将样品引入第一室中，b）进行多个循环的扩增反应，以循环进行以扩增核酸，c）转移定义的体积，第一室的体积的一部分，并且已经将核酸扩增到第二室中，并用用于扩增反应的新试剂代替转移的限定体积，d）确定在装备有第二室的第二室中扩增的核酸的浓度元素以确定浓度，以及e）重复步骤b）-d）直到在第二室中确定在一定范围内的扩增核酸的浓度。进一步公开了一种布置。

3. 发明申请

US20070184478A1 Process for detecting a plurality of target nucleic acids 审中-公开
标题翻译：用于检测多个靶核酸的方法
公开(公告)号：US20070184478A1
公开(公告)日：2007-08-09
申请号：US11702209
申请日：2007-02-05
申请人： Walter Gumbrecht , Jorn Mosner , Manfred Stanzel , Christian Zilch
发明人： Walter Gumbrecht , Jorn Mosner , Manfred Stanzel , Christian Zilch
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6837 , C12Q1/686 , C12Q2563/143 , C12Q2563/155 , C12Q2565/137
摘要： A process and a kit for detecting a plurality of target nucleic acids are disclosed. In at least one embodiment, the process and/or kit includes using primers coupled to a semi-solid phase support.
摘要翻译：公开了用于检测多个靶核酸的方法和试剂盒。在至少一个实施方案中，所述方法和/或试剂盒包括使用与半固相支持物偶联的引物。

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