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1. 发明申请

US20060194715A1 Proteins encoded by ble genes and antibiotics from the bleomycin family 审中-公开
标题翻译：来自博来霉素家族的可溶性基因和抗生素编码的蛋白质
公开(公告)号：US20060194715A1
公开(公告)日：2006-08-31
申请号：US10532834
申请日：2003-10-27
申请人： Jonathan Blackburn , Darren Hart , Ben Godber , Mike McAndrews
发明人： Jonathan Blackburn , Darren Hart , Ben Godber , Mike McAndrews
IPC分类号： G01N33/554 , C07K9/00 , A61K38/14
CPC分类号： G01N33/9446 , A61K47/6809 , C07K14/00 , G01N33/58 , G01N2415/00
摘要： The present invention provides use of a protein conjugate comprising a “ble” protein, which has specific binding properties. The protein conjugates are capable of binding reversibly to an antibiotic from the bleomycin family, which property is exploited in a variety of immobilisation methods. In preferred aspects of the invention, the conjugates are used as markers for protein expression and/or folding, or for affinity tagging. The present invention also provides a probe comprising an array of an immobilised antibody from the bleomycin family, which acts as an analyte capture moiety. In another aspect, a purification media is provided, which comprises an antibiotic of the bleomycin family as an analyte capture moiety. Also provided is a method for generating soluble forms of an insoluble protein by expressing the protein as a “ble” fusion protein and selecting in the presence of an antibiotic from the bleomycin family. In a further aspect, the “ble” protein is expressed as a fusion protein in a cell into which is introduced a labelled antibiotic of the bleomycin family, thereby allowing identification of the cellular localisation of the protein.
摘要翻译：本发明提供了包含具有特异性结合特性的“可见”蛋白质的蛋白质缀合物的用途。蛋白质缀合物能够可逆地结合来自博来霉素家族的抗生素，该特性在各种固定化方法中被利用。在本发明的优选方面，缀合物用作蛋白质表达和/或折叠或亲和标记的标记物。本发明还提供了包含来自博来霉素家族的固定化抗体阵列的探针，其作为分析物捕捉部分。另一方面，提供了一种纯化介质，其包含作为分析物捕获部分的博来霉素家族的抗生素。还提供了通过将蛋白质表达为“可见”融合蛋白并在来自博来霉素家族的抗生素存在下选择来产生可溶性形式的不溶性蛋白质的方法。在另一方面，“可溶”蛋白质在细胞中表达为融合蛋白，其中引入了博来霉素家族的标记抗生素，从而允许鉴定蛋白质的细胞定位。

2. 发明申请

US20050221308A1 Protein tag comprising a biotinylation domain and method for increasing solubility and determining folding state 有权
标题翻译：包含生物素化结构域的蛋白质标签和增加溶解度并确定折叠状态的方法
公开(公告)号：US20050221308A1
公开(公告)日：2005-10-06
申请号：US10502581
申请日：2003-01-29
申请人： Mitali Samaddar , Jonathan Blackburn , Darren Hart , Michael Dyson
发明人： Mitali Samaddar , Jonathan Blackburn , Darren Hart , Michael Dyson
IPC分类号： G01N33/68 , C07K14/47 , C07K19/00 , C12N9/00 , C12N15/00 , C12N15/62 , C12N15/67 , C12P21/02 , C12Q1/68 , C12N15/09 , C12P21/04 , G01N33/53
CPC分类号： C12N9/93 , C07K2319/23 , C07K2319/60 , C07K2319/90 , C12N15/62 , C12N15/67 , C12P21/02
摘要： The use of a tag moiety comprising a biotinylation domain, such as biotin carboxyl carrier protein (BCCP), as a protein folding marker and protein solubility enhancer in the orientated surface capture of products of heterologously expressed genes is described. Methods for increasing the solubility of proteins and determining the folded state of a protein are also disclosed. The uses and methods of the invention can be carried out in a multiplexed manner on more than one protein in the formation of libraries. In addition the nucleic acid molecule encoding the biotinylation domain of the tag moiety can be used to increase the proportion of clones in a library that express the protein of interest.
摘要翻译：描述了在异源表达基因的产物的定向表面捕获中使用包含生物素化结构域的标签部分，例如生物素羧基载体蛋白（BCCP）作为蛋白质折叠标记和蛋白质溶解度增强剂。还公开了增加蛋白质的溶解度和测定蛋白质的折叠状态的方法。本发明的用途和方法可以以多种方式在多种形式的文库形成蛋白质中进行。此外，编码标签部分的生物素化结构域的核酸分子可以用于增加表达所关注蛋白质的文库中克隆的比例。

3. 发明申请

US20060275855A1 Enzyme array and assay 审中-公开
标题翻译：酶阵列和测定
公开(公告)号：US20060275855A1
公开(公告)日：2006-12-07
申请号：US10532787
申请日：2003-10-27
申请人： Jonathan Blackburn , Jens-Oliver Koopmann
发明人： Jonathan Blackburn , Jens-Oliver Koopmann
IPC分类号： C12Q1/48 , C12Q1/37 , C12Q1/26
CPC分类号： C12Q1/00
摘要： The present invention relates to an enzyme array and assay for use with a mass spectrometer, particularly, though not exclusively, a laser desorption/ionisation, such as a MALDI mass spectrometer. It includes a method of determining the activity of an enzyme, or the effect a test compound has on the activity of the enzyme, using mass spectrometry comprising: providing a probe carrying an immobilised enzyme; optionally introducing the test compound; introducing one or more reactants to the immobilised enzyme for a time, and in a form sufficient for a reaction to take place; drying the probe; subjecting the probe to mass spectrometry; and determining the activity of the enzyme, or the effect the test compound had on the activity of the enzyme, by detecting the presence and/or absence of one or more products and/or the one or more reactants and is exemplified with reference to a kinase assay. It also provides an array for use with the method.
摘要翻译：本发明涉及用于质谱仪的酶阵列和测定法，特别是但不限于激光解吸/电离，例如MALDI质谱仪。其包括测定酶的活性的方法或测试化合物对酶的活性的影响，使用质谱法，其包括：提供携带固定化酶的探针; 任选地引入测试化合物; 将一种或多种反应物引入固定化酶一段时间，并以足以使反应发生的形式; 干燥探头; 对探针进行质谱分析; 并通过检测一种或多种产物和/或一种或多种反应物的存在和/或不存在来确定酶的活性或测试化合物对酶的活性的影响，并且参考激酶测定。它还提供了一个用于该方法的数组。

4. 发明申请

US20060024791A1 Method 审中-公开
公开(公告)号：US20060024791A1
公开(公告)日：2006-02-02
申请号：US11237597
申请日：2005-09-27
申请人： Roland Kozlowski , Michael McAndrew , Jonathan Blackburn , Michelle Mulder , Mitali Samaddar
发明人： Roland Kozlowski , Michael McAndrew , Jonathan Blackburn , Michelle Mulder , Mitali Samaddar
IPC分类号： C40B30/04 , C40B40/06
CPC分类号： C40B30/04 , G01N33/6845
摘要： The present invention relates to novel methods of producing proteins in which one or more domains are full length and correctly folded and which are each tagged at either the N— or C-terminus with one or more marker moieties and arrays containing such proteins, as well as the use of such proteins in arrays for rapid screening.

5. 发明申请

US20070206067A1 Internal vent channel in ejection head assemblies and methods relating thereto 有权
标题翻译：喷射头组件中的内部排气通道和与其相关的方法
公开(公告)号：US20070206067A1
公开(公告)日：2007-09-06
申请号：US11365193
申请日：2006-03-01
申请人： Jonathan Blackburn , Edgar Diaz , Thomas Romine , Jeanne Singh , Mary Smoot , Jason Stokesbary
发明人： Jonathan Blackburn , Edgar Diaz , Thomas Romine , Jeanne Singh , Mary Smoot , Jason Stokesbary
IPC分类号： B41J2/175
CPC分类号： B41J2/1753
摘要： Fluid ejection head assemblies, fluid ejection devices, and methods for improving fluid sealing of fluid ejection head assemblies. One such fluid ejection head assembly includes a substrate cavity and a substantially planar surface surrounding the substrate cavity. The substantially planar surface contains at least one external vent, at least one internal vent channel, and a plurality of vents in fluid flow communication with the substrate cavity and providing fluid flow communication between the internal vent channel and the external vent. The plurality of vents, the at least one external vent and the at least one internal vent channel are disposed in fluid flow communication with an environment external to the substrate cavity for flow of a gas associated with an adhesive at least partially disposed in the substrate cavity, to the environment during the curing of the adhesive.
摘要翻译：流体喷射头组件，流体喷射装置和用于改善流体喷射头组件的流体密封的方法。一个这样的流体喷射头组件包括衬底空腔和围绕衬底空腔的基本平坦的表面。基本平坦的表面包含至少一个外部通气口，至少一个内部通气通道和与衬底空腔流体连通的多个通气口，并且在内部通气通道和外部通风口之间提供流体流动连通。多个通气口，至少一个外部通气口和至少一个内部通气通道设置成与衬底空腔外部的环境流体流动连通，用于与至少部分地设置在衬底空腔中的粘合剂相关联的气体流动，在粘合剂固化期间的环境。

6. 发明申请

US20050196791A1 Probe for mass spectrometry 审中-公开
标题翻译：探测质谱
公开(公告)号：US20050196791A1
公开(公告)日：2005-09-08
申请号：US11054050
申请日：2005-02-08
申请人： Jens-Oliver Koopman , Jonathan Blackburn
发明人： Jens-Oliver Koopman , Jonathan Blackburn
IPC分类号： G01N27/64 , A61K47/48 , C07K14/00 , G01N27/62 , G01N33/483 , G01N33/53 , G01N33/94 , G01N37/00 , H01J49/04 , H01J49/16 , C12Q1/68 , C12M1/34 , H01J49/00
CPC分类号： H01J49/0418 , A61K47/6809 , B82Y30/00 , C07K14/00 , G01N33/9446 , G01N2415/00 , Y10T436/24
摘要： The present invention relates to a probe for the analysis of one or more analytes, particularly proteins or compounds capable of binding or otherwise interacting therewith, by laser desorption/ionisation mass spectrometry, more particularly MALDI MS. It also relates to a protein microarray, a method of producing a protein microarray and a method of analysing a protein microarray. The probe comprises a support having an electroconductive target surface thereon characterized in that the target surface comprises a micro array having a plurality of discrete target areas presenting one or more analyte capture moieties. Each discrete target area has an area of less than 1000 μm2, more preferably still less than 500 μm2, and more preferably still less than 100 μm2.
摘要翻译：本发明涉及通过激光解吸/电离质谱法，更具体地说是MALDI MS分析一种或多种分析物，特别是能够结合或以其它方式相互作用的蛋白质或化合物的探针。它还涉及蛋白质微阵列，产生蛋白质微阵列的方法和分析蛋白质微阵列的方法。探针包括其上具有导电目标表面的支撑体，其特征在于，目标表面包括具有呈现一个或多个分析物捕获部分的多个离散靶区域的微阵列。每个离散目标区域的面积小于1000μm2，更优选小于500μm2，更优选小于100μm2， / SUP>。

7. 发明申请

US20050181449A1 Arrays and methods 审中-公开
标题翻译：数组和方法
公开(公告)号：US20050181449A1
公开(公告)日：2005-08-18
申请号：US10506756
申请日：2003-03-13
申请人： Roland Kozlowski , Jonathan Blackburn
发明人： Roland Kozlowski , Jonathan Blackburn
IPC分类号： C07K1/107 , C07K14/705 , C07K14/72 , G01N33/53 , C07K16/18 , G01N33/543
CPC分类号： C07K14/723 , C07K1/1077 , C07K14/705
摘要： Arrays of cytosolic accessory proteins are provided, together with methods of screening, using such arrays.
摘要翻译：提供了细胞溶质辅助蛋白的阵列，以及使用这种阵列的筛选方法。

8. 发明申请

US20060199220A1 Protein arrays and uses thereof 审中-公开
标题翻译：蛋白质阵列及其用途
公开(公告)号：US20060199220A1
公开(公告)日：2006-09-07
申请号：US10527603
申请日：2003-09-16
申请人： Jonathan Blackburn , Benjamin Godber , Darren Hart , Nicholas Bockett , Roland Kozlowski , Michael Dyson
发明人： Jonathan Blackburn , Benjamin Godber , Darren Hart , Nicholas Bockett , Roland Kozlowski , Michael Dyson
IPC分类号： G01N33/53 , C12Q1/26 , C12M1/34
CPC分类号： C12Q1/00 , B01J2219/00387 , B01J2219/00527 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00628 , B01J2219/00639 , B01J2219/00691 , B01J2219/00722 , B01J2219/00725 , C12Q1/26 , C12Q1/6837 , C40B30/04 , C40B40/06 , C40B40/10 , C40B60/14 , G01N2333/90209 , G01N2500/00
摘要： The Inventors herein describe methods for the production of a functional human, animal, plant or microbe protein arrays and methods to assay for interactions between the proteins on the array with molecules of interest, for example, using such arrays to determine the in vitro metabolite profile of any drug. Such protein arrays can be used, for example, to assay, in a parallel fashion, the protein products of DNA sequences encoding drug metabolising enzymes (DMEs) to obtain a toxicology profile. Also described herein is a novel DME expression and purification strategy using detergents and not requiring an ultra-centrifugation step.
摘要翻译：本文的发明人描述了生产功能性人，动物，植物或微生物蛋白质阵列的方法以及用于测定阵列上的蛋白质与感兴趣的分子之间的相互作用的方法，例如使用这样的阵列来确定体外代谢物谱的任何药物这样的蛋白质阵列可以用于例如以并行方式测定编码药物代谢酶（DME）的DNA序列的蛋白质产物，以获得毒理学谱。本文还描述了使用洗涤剂并且不需要超离心步骤的新型DME表达和纯化策略。

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