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1. 发明申请

US20140318963A1 METHODS FOR IONOPHORICALLY SCREENING PORE FORMING BACTERIAL PROTEIN TOXINS AND RECEPTORS 审中-公开
标题翻译：方法筛选形成细菌蛋白毒素和受体的孔
公开(公告)号：US20140318963A1
公开(公告)日：2014-10-30
申请号：US14327897
申请日：2014-07-10
申请人： John Cuppoletti
发明人： John Cuppoletti
IPC分类号： G01N27/447
CPC分类号： G01N27/447 , G01N33/56911 , G01N33/68 , G01N2400/50 , G01N2500/20 , Y10S435/975 , Y10T428/249953
摘要： One embodiment of the present invention is directed to methods for ionophorically screening pore forming bacterial protein toxins and receptors. The method includes: a) forming a membrane comprising a lipid and a receptor, b) contacting the membrane with the pore forming bacterial protein toxin and an ion solution, and c) measuring ion flow through the membrane.
摘要翻译：本发明的一个实施方案涉及用于离子色谱筛选造孔细菌蛋白质毒素和受体的方法。该方法包括：a）形成包含脂质和受体的膜，b）使所述膜与形成孔的细菌蛋白质毒素和离子溶液接触，以及c）测量通过膜的离子流。

2. 发明授权

US5208649A Assembly for front face testing 失效
标题翻译：组装面前测试
公开(公告)号：US5208649A
公开(公告)日：1993-05-04
申请号：US839469
申请日：1992-02-20
申请人： John Cuppoletti , Nick H. Langdon
发明人： John Cuppoletti , Nick H. Langdon
IPC分类号： G01N21/01 , G01N21/03
CPC分类号： G01N21/01 , G01N2021/0367 , G01N2021/6476
摘要： A coverslip holder assembly for a front face fluorimetry flow cell has a holder body and a hingedly attached clamp for releasably maintaining a coverslip adjacent the front face of the holder body and flow cell. The assembly is preferably a unitary arrangement wherein the clamp is hingedly mounted adjacent the bottom end of the front face of the holder body, and has a convenient snap lock release mechanism at its top end to selectively lock the coverslip in flush arrangement with the front face. The holder assembly can provide a biased snug fit with a variety of cuvettes or similar sample vessels, and enables the use of highly colored or fluorescent compounds for perfusion in front face fluorimetry operations.
摘要翻译：用于前表面荧光测定流动池的盖玻片保持器组件具有保持器主体和铰接附接的夹具，用于可拆卸地保持邻近保持器本体和流动池的前表面的盖玻片。组件优选地是一体的布置，其中夹具相对于保持器主体的前表面的底端铰接地安装，并且在其顶端具有方便的卡扣释放机构，以选择性地将盖玻片与前面齐平地安装。保持器组件可以提供与各种比色皿或类似样品容器的偏置贴合配合，并且能够在前面荧光测定操作中使用高度着色或荧光化合物用于灌注。

3. 发明授权

US08877517B1 Methods for ionophorically screening pore forming bacterial protein toxins and receptors 有权
标题翻译：用于离子色谱筛选形成细菌的细菌蛋白毒素和受体的方法
公开(公告)号：US08877517B1
公开(公告)日：2014-11-04
申请号：US14327897
申请日：2014-07-10
申请人： John Cuppoletti
发明人： John Cuppoletti
IPC分类号： C12M1/00 , C12M1/34 , G01N33/543 , G01N27/447
CPC分类号： G01N27/447 , G01N33/56911 , G01N33/68 , G01N2400/50 , G01N2500/20 , Y10S435/975 , Y10T428/249953
摘要： A method for determining the amount of pore forming bacterial toxin protein in a sample is provided, the method including the steps of a) forming a membrane comprising a lipid bilayer and a receptor, b) contacting the membrane with an ion solution and the sample, c) measuring ion flow through the membrane, d) comparing the ion flow through the membrane to a standard curve, and e) determining the amount of pore forming bacterial toxin protein in the sample.
摘要翻译：提供了一种用于确定样品中形成细菌毒素蛋白质的量的方法，所述方法包括以下步骤：a）形成包含脂质双层和受体的膜，b）使膜与离子溶液和样品接触， c）测量通过膜的离子流，d）将通过膜的离子流与标准曲线进行比较，e）确定样品中形成细菌毒素蛋白的量。

4. 发明申请

US20080207759A1 METHOD FOR PROTECTING MITOCHONDRIA 审中-公开
标题翻译：保护麻醉方法
公开(公告)号：US20080207759A1
公开(公告)日：2008-08-28
申请号：US12037435
申请日：2008-02-26
申请人： Ryuji UENO , Sachiko Kuno , John Cuppoletti
发明人： Ryuji UENO , Sachiko Kuno , John Cuppoletti
IPC分类号： A61K31/5575 , A61P43/00
CPC分类号： A61K31/5575
摘要： The present invention relates to a method for protecting mitochondria from damage in a mammalian subject, which comprises administering an effective amount of a prostaglandin compound to a subject in need thereof. Also provided is a method for treating mitochondrial dysfunction as well as a condition associated with mitochondrial dysfunction in a mammalian subject, which comprises administering an effective amount of a prostaglandin compound to a subject in need thereof.
摘要翻译：本发明涉及一种保护线粒体免受哺乳动物受试者损伤的方法，其包括向有需要的受试者施用有效量的前列腺素化合物。还提供了治疗线粒体功能障碍的方法以及与哺乳动物受试者的线粒体功能障碍相关的病症，其包括向有需要的受试者施用有效量的前列腺素化合物。

5. 发明授权

US07064148B2 Chloride channel opener 有权
公开(公告)号：US07064148B2
公开(公告)日：2006-06-20
申请号：US10231341
申请日：2002-08-30
申请人： Ryuji Ueno , John Cuppoletti
发明人： Ryuji Ueno , John Cuppoletti
IPC分类号： A61K31/19
CPC分类号： C07C405/00 , A61K31/557
摘要： Disclosed is a novel use of a prostaglandin compound as a chloride channel opener. According to the instant invention, chloride channels in a mammalian subject can be opened by a prostaglandin compound to facilitate chloride ion transportation.

6. 发明申请

US20060068503A1 Solid support membranes for ion channel arrays and sensors: application ro rapid screening of pharmacological compounds 审中-公开
标题翻译：用于离子通道阵列和传感器的固体支持膜：药物化合物的应用快速筛选
公开(公告)号：US20060068503A1
公开(公告)日：2006-03-30
申请号：US11224422
申请日：2005-09-12
申请人： John Cuppoletti
发明人： John Cuppoletti
IPC分类号： G01N33/543
CPC分类号： G01N33/6872 , G01N33/5438 , G01N33/553
摘要： The present invention relates to synthetic membranes and provides a solid supported membrane comprising: a support; a chromium layer; a gold layer; an alkyl monolayer, a lipid monolayer; and an ion channel protein, wherein the alkyl monolayer and the lipid monolayer form a bilayer, and further wherein a functionally reconstituted ion channel is supported, and methods for forming them. The invention further provides methods and applications of the membranes in rapid screening for pharmacological agents and as bioelectric smart sensors.
摘要翻译：本发明涉及合成膜并提供固体支撑膜，其包括：载体; 铬层; 金层; 烷基单层，脂质单层; 和离子通道蛋白质，其中所述烷基单层和所述脂质单层形成双层，并且其中支持功能重构的离子通道及其形成方法。本发明进一步提供膜快速筛选药理学和生物电智能传感器的方法和应用。

7. 发明授权

US08835181B2 Methods for ionophorically screening pore forming bacterial protein toxins and receptors 有权
标题翻译：用于离子色谱筛选形成细菌的细菌蛋白毒素和受体的方法
公开(公告)号：US08835181B2
公开(公告)日：2014-09-16
申请号：US13721425
申请日：2012-12-20
申请人： John Cuppoletti
发明人： John Cuppoletti
IPC分类号： G01N33/53 , C12M1/00 , C12M1/34 , G01N33/68 , G01N33/569
CPC分类号： G01N27/447 , G01N33/56911 , G01N33/68 , G01N2400/50 , G01N2500/20 , Y10S435/975 , Y10T428/249953
摘要： A method for determining the amount of live pore forming bacterial toxin protein in a sample is provided, the method including the steps of a) forming a membrane comprising a lipid bilayer and a receptor, b) contacting the membrane with an ion solution and the sample, c) measuring ion flow through the membrane, d) comparing the ion flow through the membrane to a standard curve, and e) determining the amount of pore forming bacterial toxin protein in the sample. A kit for determining the amount of live pore forming bacterial toxin protein present in the sample is also provided.
摘要翻译：提供了一种用于确定样品中活细菌形成细菌毒素蛋白质的量的方法，所述方法包括以下步骤：a）形成包含脂质双层和受体的膜，b）使膜与离子溶液和样品接触 c）测量通过膜的离子流，d）将通过膜的离子流与标准曲线进行比较，e）确定样品中形成细菌毒素蛋白的量。还提供了用于确定样品中存在的活细孔形成细菌毒素蛋白的量的试剂盒。

8. 发明授权

US08361809B2 Methods for ionophorically screening pore forming bacterial protein toxins and receptors 有权
标题翻译：用于离子色谱筛选形成细菌的细菌蛋白毒素和受体的方法
公开(公告)号：US08361809B2
公开(公告)日：2013-01-29
申请号：US13368861
申请日：2012-02-08
申请人： John Cuppoletti
发明人： John Cuppoletti
IPC分类号： C12M1/00 , C12M1/34 , G01N33/543
CPC分类号： G01N27/447 , G01N33/56911 , G01N33/68 , G01N2400/50 , G01N2500/20 , Y10S435/975 , Y10T428/249953
摘要： One embodiment of the present invention is directed to methods for ionophorically screening pore forming bacterial protein toxins and receptors for insect toxicity. The method includes: a) forming a membrane comprising a lipid and an insect receptor, b) contacting the membrane with the pore forming bacterial protein toxin and an ion solution, and c) measuring ion flow through the membrane. Also provided are a method and kit for determining the amount of live pore forming bacterial toxin protein in a sample.
摘要翻译：本发明的一个实施方案涉及用于电离屏蔽成孔细菌蛋白质毒素和昆虫毒性的受体的方法。该方法包括：a）形成包含脂质和昆虫受体的膜，b）使所述膜与形成孔的细菌蛋白质毒素和离子溶液接触，以及c）测量通过膜的离子流。还提供了用于测定样品中活细孔形成细菌毒素蛋白的量的方法和试剂盒。

9. 发明授权

US07833805B2 Selectively permeable membranes on porous substrates 有权
标题翻译：多孔基材上的选择性渗透膜
公开(公告)号：US07833805B2
公开(公告)日：2010-11-16
申请号：US11454569
申请日：2006-06-16
申请人： John Cuppoletti
发明人： John Cuppoletti
IPC分类号： C12M1/00 , C12M1/34 , G01N33/543 , B32B3/26 , B01L99/00
CPC分类号： B01D69/10 , B01D69/144 , B01D71/06 , H01B1/122 , H01M8/16 , H01M2300/0082 , H01M2300/0094 , Y02E60/527 , Y02P70/56 , Y10T428/249953
摘要： Functional biological synthetic composite (BSC) membranes comprising phospholipids, biological membrane proteins and porous supports or membranes are provided. Lipid bilayers are formed on porous polycarbonate (PC), polyethylene terephthalate (PETE) and poly (I-lactic acid) (PLLA) membranes and in laser-drilled pores in a multi-well plastic plate as measured by increased resistance or decreased currents. BSC's comprising functional reconstituted Kv1.5 K channel and/or H/K ATPase transport proteins are also provided c inhibitor), methods of manufacture, and high throughput screening assays employing the inventive membranes are also provided.
摘要翻译：提供了包含磷脂，生物膜蛋白质和多孔载体或膜的功能性生物合成复合物（BSC）膜。在多孔聚碳酸酯（PC），聚对苯二甲酸乙二醇酯（PETE）和聚（l-乳酸）（PLLA）膜上形成脂质双层，并在多孔塑料板中的激光钻孔中形成电阻增加或电流降低测量。还提供包含功能重构的Kv1.5K通道和/或H / K ATP酶转运蛋白的BSC，c抑制剂），使用本发明的膜的制备方法和高通量筛选测定。

10. 发明授权

US07253295B2 Chloride channel opener 有权
标题翻译：氯化物通道开启剂
公开(公告)号：US07253295B2
公开(公告)日：2007-08-07
申请号：US11333511
申请日：2006-01-18
申请人： Ryuji Ueno , John Cuppoletti
发明人： Ryuji Ueno , John Cuppoletti
IPC分类号： C07C23/00
CPC分类号： C07C405/00 , A61K31/557
摘要： Disclosed is a novel use of a prostaglandin compound as a chloride channel opener. According to the instant invention, chloride channels in a mammalian subject can be opened by a prostaglandin compound to facilitate chloride ion transportation.
摘要翻译：公开了前列腺素化合物作为氯化物通道开放剂的新用途。根据本发明，哺乳动物受试者中的氯化物通道可以被前列腺素化合物打开以促进氯离子转运。

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