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1. 发明申请

US20100194409A1 METHOD OF ELECTRICALLY DETECTING A BIOLOGICAL ANALYTE MOLECULE 审中-公开
标题翻译：电生物分析仪分析方法
公开(公告)号：US20100194409A1
公开(公告)日：2010-08-05
申请号：US12377404
申请日：2007-08-14
申请人： Zhiqiang Gao , Yi Fan , Xiantong Chen , Jinming Kong
发明人： Zhiqiang Gao , Yi Fan , Xiantong Chen , Jinming Kong
IPC分类号： G01R27/08 , C01B31/00
CPC分类号： G01N33/5438 , C12Q1/6825 , C12Q2565/607 , C12Q2563/155
摘要： The invention provides a method of electrically detecting a biological analyte molecule by means of a pair of electrodes. The electrodes are arranged at a distance from one another within a sensing zone. A capture molecule, which has an affinity to the analyte molecule and which is capable of forming a complex with the analyte molecule, is immobilised on an immobilisation unit. The immobilisation unit is contacted with a solution suspected to comprise the analyte molecule. The analyte molecule is allowed to form a complex with the capture molecule. The invention also provides a probe defined by a nanoparticulate tag that comprises or consists of electrically conducting matter that is capable of chemically interacting with the analyte molecule. In the method of the invention the electrically conducting nanoparticulate tag is added. Thereby the electrically conducting nanoparticulate tag is allowed to associate to the complex formed between the capture molecule and the analyte molecule. The presence of the analyte molecule is determined based on an electrical characteristic, influenced by the electrically conducting nanoparticulate tag, of a region in the sensing zone.
摘要翻译：本发明提供一种通过一对电极电生物分析物分子的电学检测方法。电极在感测区内相互间隔一定距离。捕获分子与分析物分子具有亲和性，并且能够与分析物分子形成复合物，被固定在固定化单元上。固定化单元与疑似包含分析物分子的溶液接触。允许分析物分子与捕获分子形成复合物。本发明还提供由纳米颗粒标签定义的探针，其包含能够与分析物分子进行化学相互作用的导电物质或由导电物质组成。在本发明的方法中，添加导电纳米颗粒标签。由此，导电纳米颗粒标签被允许与形成在捕获分子和分析物分子之间的复合物结合。分析物分子的存在基于感测区域中的区域的导电纳米颗粒标签的电特性来确定。

2. 发明申请

US20100285601A1 METHOD OF ELECTRICALLY DETECTING A NUCLEIC ACID MOLECULE 审中-公开
标题翻译：电流检测核酸分子的方法
公开(公告)号：US20100285601A1
公开(公告)日：2010-11-11
申请号：US12680290
申请日：2007-09-28
申请人： Jinming Kong , Yi Fan , Xiantong Chen
发明人： Jinming Kong , Yi Fan , Xiantong Chen
IPC分类号： G01N27/00
CPC分类号： G01N33/54306 , Y10T436/143333
摘要： The method is performed by means of a pair of electrodes that are arranged at a distance and within a sensing zone. A nucleic acid capture molecule with an uncharged backbone and a nucleotide sequence that is at least partially complementary to at least a portion of a strand of the target nucleic acid molecule, is immobilised on an immobilisation unit. The immobilisation unit, which is arranged within the sensing zone, is contacted with a solution suspected to comprise the target nucleic acid molecule, which hybridizes to the nucleic acid capture molecule. An activation agent is added, which has an electrostatic net charge complementary to the net charge of the target nucleic acid molecule. It associates to the complex of nucleic acid capture molecule and target nucleic acid molecule. Added is a water soluble polymer with at least one polymer strand and with an electrostatic net charge that is complementary to the net charge of the activation agent. Thus the polymer associates to the activation agent. A metal salt is added, which can act as an oxidant and the metal ions of which have an electrostatic net charge complementary to the net charge of the polymer. The metal ions associate to the polymer. Upon adding a reducing agent, the latter reduces the metal ions, forming a metal wire. The presence of the analyte molecule is determined based on an electrical characteristic of a region in the sensing zone that is affected by the metal wire.
摘要翻译：该方法通过一对电极进行，该对电极被布置在距离和感测区域内。具有不带电荷的骨架的核酸捕获分子和与靶核酸分子的链的至少一部分至少部分互补的核苷酸序列被固定在固定化单元上。布置在感测区内的固定单元与疑似包含与核酸捕获分子杂交的靶核酸分子的溶液接触。加入活化剂，其具有与靶核酸分子的净电荷互补的静电净电荷。它与核酸捕获分子和靶核酸分子的复合物结合。添加的是具有至少一个聚合物链和具有与活化剂的净电荷互补的静电净电荷的水溶性聚合物。因此，聚合物与活化剂结合。加入金属盐，其可以用作氧化剂，并且其金属离子具有与聚合物的净电荷互补的静电净电荷。金属离子与聚合物缔合。加入还原剂后，还原金属离子，形成金属丝。分析物分子的存在基于受金属线影响的感测区域中的区域的电特性来确定。

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