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    • 4. 发明授权
    • Method for recovering sperm nucleic acid from a forensic sample
    • 从法医样品中回收精子核酸的方法
    • US08969044B2
    • 2015-03-03
    • US12177579
    • 2008-07-22
    • Jason Yingjie Liu
    • Jason Yingjie Liu
    • C12P19/34C12N15/10
    • C12Q1/6806C12N15/1003
    • A method for selectively recovering nucleic acid from a sperm cell in a sample containing cells of at least a sperm cell and an epithelial cell, and a cell suspension medium comprising extracellular impurities, is provided. The method entails introducing a sample into a vessel, sequestering the cells from the remaining sample components, washing the cells with a washing solution either before or after sequestration, removing the impurities-containing cell suspension medium from the vessel while retaining the cells; lysing selectively cells of the first cell type; and isolating the nucleic acid from the lysed cells. Methods for recovering nucleic acid from the second cell type are also provided.
    • 提供了选择性地从含有至少精细胞和上皮细胞的细胞的样品中的精子细胞中回收核酸的方法和包含细胞外杂质的细胞悬浮培养基。 该方法需要将样品引入容器中,从剩余的样品组分中隔离细胞,在封存之前或之后用洗涤溶液洗涤细胞,同时保留细胞,从容器中除去含杂质的细胞悬浮培养基; 选择性裂解第一细胞类型的细胞; 并从裂解的细胞中分离核酸。 还提供了从第二细胞类型中回收核酸的方法。
    • 6. 发明申请
    • METHOD FOR RECOVERING SPERM NUCLEIC ACID FROM A FORENSIC SAMPLE
    • 从威尔逊样品中回收孢子核酸的方法
    • US20090042255A1
    • 2009-02-12
    • US12177579
    • 2008-07-22
    • Jason Yingjie Liu
    • Jason Yingjie Liu
    • C12P19/34
    • C12Q1/6806C12N15/1003
    • A method for selectively recovering nucleic acid from a sperm cell in a sample containing cells of at least a sperm cell and an epithelial cell, and a cell suspension medium comprising extracellular impurities, is provided. The method entails introducing a sample into a vessel, sequestering the cells from the remaining sample components, washing the cells with a washing solution either before or after sequestration, removing the impurities-containing cell suspension medium from the vessel while retaining the cells; lysing selectively cells of the first cell type; and isolating the nucleic acid from the lysed cells. Methods for recovering nucleic acid from the second cell type are also provided.
    • 提供了选择性地从含有至少精细胞和上皮细胞的细胞的样品中的精子细胞中回收核酸的方法和包含细胞外杂质的细胞悬浮培养基。 该方法需要将样品引入容器中,从剩余的样品组分中隔离细胞,在封存之前或之后用洗涤溶液洗涤细胞,同时保留细胞,从容器中除去含杂质的细胞悬浮培养基; 选择性裂解第一细胞类型的细胞; 并从裂解的细胞中分离核酸。 还提供了从第二细胞类型中回收核酸的方法。