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    • 1. 发明申请
    • IMAGING OF BIOLOGICAL SAMPLES
    • 生物样本的成像
    • US20090173892A1
    • 2009-07-09
    • US12159790
    • 2007-01-19
    • Patrick CourtneyPaul OrangeJanet ParkClaire Hooper
    • Patrick CourtneyPaul OrangeJanet ParkClaire Hooper
    • G01T1/10G01J1/58
    • G01N33/582G01N21/6408G01N21/6428G01N21/6458G01N33/5005
    • Methods and apparatus relating to the imaging of biological samples are provided. More particularly, they relate to the detection of light emanating from fluorescent species present in a sample in order to study the structure and dynamics of such a sample. Such a method of analysis comprises irradiating the sample with a pulse of excitation energy causing fluorescent species in the sample to fluoresce; detecting light emanating from the sample during a predetermined period of time after the pulse; generating and storing data recording at least the wavelength of the detected light against time; and analysing the data with reference to the respective lifetimes of the fluorescent species to detect the presence of the respective emissions from three or more different fluorescent species which emit light simultaneously during at least part of said predetermined period, which are indistinguishable from each other on the basis of their wavelength or lifetime alone.
    • 提供了与生物样品成像有关的方法和设备。 更具体地,它们涉及从存在于样品中的荧光物质发出的光的检测,以便研究这种样品的结构和动力学。 这种分析方法包括用激发能量的脉冲照射样品中的荧光物质发荧光; 在脉冲之后的预定时间段内检测从样品发出的光; 生成和存储至少记录检测到的光的波长的数据; 并且参考荧光物质的相应寿命来分析数据,以检测来自三个或更多个在所述预定时间段的至少部分期间同时发光的三种或更多种不同荧光物质的各自发射的存在,所述荧光物质在 它们的波长或寿命的基础。
    • 2. 发明授权
    • Imaging of biological samples
    • 生物样品成像
    • US07906768B2
    • 2011-03-15
    • US12159790
    • 2007-01-19
    • Patrick CourtneyPaul OrangeJanet ParkClaire Hooper
    • Patrick CourtneyPaul OrangeJanet ParkClaire Hooper
    • G01J1/58
    • G01N33/582G01N21/6408G01N21/6428G01N21/6458G01N33/5005
    • Methods and apparatus relating to the imaging of biological samples are provided. More particularly, they relate to the detection of light emanating from fluorescent species present in a sample in order to study the structure and dynamics of such a sample. Such a method of analysis comprises irradiating the sample with a pulse of excitation energy causing fluorescent species in the sample to fluoresce; detecting light emanating from the sample during a predetermined period of time after the pulse; generating and storing data recording at least the wavelength of the detected light against time; and analysing the data with reference to the respective lifetimes of the fluorescent species to detect the presence of the respective emissions from three or more different fluorescent species which emit light simultaneously during at least part of said predetermined period, which are indistinguishable from each other on the basis of their wavelength or lifetime alone.
    • 提供了与生物样品成像有关的方法和设备。 更具体地,它们涉及从存在于样品中的荧光物质发出的光的检测,以便研究这种样品的结构和动力学。 这种分析方法包括用激发能量的脉冲照射样品中的荧光物质发荧光; 在脉冲之后的预定时间段内检测从样品发出的光; 生成和存储至少记录检测到的光的波长的数据; 并且参考荧光物质的相应寿命来分析数据,以检测来自三个或更多个不同荧光物质的各自发射的存在,所述荧光物质在所述预定时间段的至少部分期间同时发光,所述不同荧光物质在 它们的波长或寿命的基础。