专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08957036B2 Targeted delivery of glycine receptors to excitable cells 有权
标题翻译：将甘氨酸受体靶向递送至可兴奋细胞
公开(公告)号：US08957036B2
公开(公告)日：2015-02-17
申请号：US13043195
申请日：2011-03-08
申请人： Michael Cascio , Joseph C. Glorioso, III , James R. Goss , David Krisky
发明人： Michael Cascio , Joseph C. Glorioso, III , James R. Goss , David Krisky
IPC分类号： A61K48/00 , C07K14/705 , C12N15/86 , A61K38/00
CPC分类号： C07K14/705 , A01K67/0278 , A01K2217/20 , A01K2217/206 , A01K2227/105 , A01K2267/0356 , A01K2267/0393 , A61K38/00 , A61K48/005 , C12N7/00 , C12N15/86 , C12N2710/16643 , C12N2800/30
摘要： The invention provides a method of modulating electrophysiological activity of an excitable cell. The method involves causing exogenous expression of a glycine receptor (GlyR) protein in an excitable cell of a subject. Thereafter, the excitable cell is exposed to an allosteric modulator of the GlyR protein. Modulation of the exogenous GlyR protein (an ion channel) in response to the allosteric modulator modulates the electrophysiological activity of the excitable cell. The method can be used to control pain in a subject. The invention further provides a replication-defective HSV vector comprising an expression cassette encoding a GlyR protein, stocks and pharmaceutical compositions containing such vectors, and a transgenic animal.
摘要翻译：本发明提供调节可兴奋细胞的电生理活性的方法。该方法涉及在受试者的可兴奋细胞中引起甘氨酸受体（GlyR）蛋白质的外源表达。此后，将可兴奋细胞暴露于GlyR蛋白质的变构调节剂。响应于变构调节因子调节外源性GlyR蛋白（离子通道）调节可兴奋细胞的电生理活性。该方法可用于控制受试者的疼痛。本发明还提供了包含编码GlyR蛋白的表达盒，含有这些载体的原料和药物组合物和转基因动物的复制缺陷型HSV载体。

2. 发明授权

US08309349B2 Cell line 有权
标题翻译：细胞系
公开(公告)号：US08309349B2
公开(公告)日：2012-11-13
申请号：US12418062
申请日：2009-04-03
申请人： Joseph C. Glorioso , Darren Wolfe , David Krisky
发明人： Joseph C. Glorioso , Darren Wolfe , David Krisky
IPC分类号： C12N5/00 , C12N15/00
CPC分类号： C12N15/86 , C12N7/00 , C12N9/88 , C12N2710/16643 , C12N2840/20 , C12Y401/01015
摘要： The invention provides a cell line complementing HSV ICP4, ICP27, and UL55 genes.
摘要翻译：本发明提供补充HSV ICP4，ICP27和UL55基因的细胞系。

3. 发明授权

US10174341B2 Non-toxic HSV vectors for efficient gene delivery applications and complementing cells for their production 有权
公开(公告)号：US10174341B2
公开(公告)日：2019-01-08
申请号：US14905708
申请日：2014-07-17
申请人： UNIVERSITY OF PITTSBURGH—OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION , Joseph C. Glorioso, III , Justus Cohen , Yoshitaka Miyagawa , David Krisky , James Wechuck , Darren Wolfe
发明人： Joseph C. Glorioso, III , Justus Cohen , Yoshitaka Miyagawa , David Krisky , James Wechuck , Darren Wolfe
IPC分类号： C12N5/00 , C12N15/86 , A61K35/763 , A61K48/00 , C12N7/00
摘要： The invention provides a herpes simplex virus (HSV) vector that does not express toxic HSV genes in non-complementing cells and which comprises a genome comprising one or more transgenes, wherein the vector is capable of expression of a transgene for at least 28 days in non-complementing cells. The disclosed vectors include vectors having deletions in the genes ICP0, ICP4, TCP22, TCP27 and TCP47, or alternative inactivating mutations, or vectors which express one or more of these genes with modified kinetics. The invention also relates to viral stocks of the inventive vectors, compositions thereof suitable for use therapeutically or for in vitro applications, and methods relating thereto. In another aspect, the invention provides a complementing cell, in particular a U20S cell, engineered to express ICP4 and ICP27 when the cell is infected with HSV for the production of the inventive vector. Said cells are disclosed as naturally complementing ICP0.

4. 发明授权

US08846889B2 Peptide biosynthesis and pain therapy 有权
标题翻译：肽生物合成和疼痛治疗
公开(公告)号：US08846889B2
公开(公告)日：2014-09-30
申请号：US12893352
申请日：2010-09-29
申请人： Darren P. Wolfe , Joseph C. Glorioso , David J. Fink
发明人： Darren P. Wolfe , Joseph C. Glorioso , David J. Fink
IPC分类号： C07H21/04 , A61K31/711 , C12N15/12 , C12N15/62 , A61K48/00 , G01N33/68 , C07K14/67 , C07K5/107 , C12N15/86 , G01N33/94 , C07K7/22 , C07K14/695 , C07K14/70
CPC分类号： C07K5/1016 , A61K48/005 , C07K7/22 , C07K14/67 , C07K14/695 , C07K14/70 , C07K2319/50 , C07K2319/60 , C12N15/86 , C12N2710/16643 , G01N33/6893 , G01N33/9486 , G01N2800/2842
摘要： The invention provides an expression cassette comprising a DNA sequence encoding amino acids 1-99 of human preproenkephalin, a DNA sequence encoding a precursor of a carboxy-amidated peptide flanked by dibasic cleavage sites and optionally a DNA sequence encoding a marker protein (such as Enhanced Green Fluorescent Protein (GNP)) all in operable linkage and under control of a promoter. Where the encoded precursor of a carboxy-amidated peptide is an agonist for an opioid receptor, the invention further provides a method of treating neuropathic pain by administering the gene transfer vector comprising such an expression cassette to a patient. The invention also provides a method for detecting a peptide having a desired effect comprising introducing a library of DNA sequences encoding one or more precursors of carboxy-amidated peptides into host cells; expressing the carboxy-amidated peptides encoded in the library to provide expression products; and screening from the polypeptide expression products for the desired effect.
摘要翻译：本发明提供了一种表达盒，其包含编码人前脑啡肽的氨基酸1-99的DNA序列，编码侧翼为二碱基切割位点的羧基酰胺化肽的前体的DNA序列和任选的编码标记蛋白的DNA序列（例如增强型绿色荧光蛋白（GNP））均可操作地连接并在启动子的控制下。当羧基酰胺化肽的编码前体是阿片受体的激动剂时，本发明还提供了通过将包含这种表达盒的基因转移载体给予患者来治疗神经性疼痛的方法。本发明还提供了一种检测具有所需效果的肽的方法，包括将编码羧基酰胺化肽的一种或多种前体的DNA序列文库导入宿主细胞; 表达在文库中编码的羧基酰胺化肽以提供表达产物; 并从多肽表达产物筛选出所需的效果。

5. 发明申请

US20080289058A1 Targeted delivery of glycine receptors to excitable cells 审中-公开
标题翻译：将甘氨酸受体靶向递送至可兴奋细胞
公开(公告)号：US20080289058A1
公开(公告)日：2008-11-20
申请号：US12152310
申请日：2008-05-14
申请人： Michael Cascio , Joseph C. Glorioso, III , James R. Goss , David Krisky
发明人： Michael Cascio , Joseph C. Glorioso, III , James R. Goss , David Krisky
IPC分类号： A01K67/027 , C12N5/06 , A61P25/02 , A61K31/7088
CPC分类号： C07K14/705 , A01K67/0278 , A01K2217/20 , A01K2217/206 , A01K2227/105 , A01K2267/0356 , A01K2267/0393 , A61K38/00 , A61K48/005 , C12N7/00 , C12N15/86 , C12N2710/16643 , C12N2800/30
摘要： The invention provides a method of modulating electrophysiological activity of an excitable cell. The method involves causing exogenous expression of a glycine receptor (GlyR) protein in an excitable cell of a subject. Thereafter, the excitable cell is exposed to an allosteric modulator of the GlyR protein. Modulation of the exogenous GlyR protein (an ion channel) in response to the allosteric modulator modulates the electrophysiological activity of the excitable cell. The method can be used to control pain in a subject. The invention further provides a replication-defective HSV vector comprising an expression cassette encoding a GlyR protein, stocks and pharmaceutical compositions containing such vectors, and a transgenic animal.
摘要翻译：本发明提供调节可兴奋细胞的电生理活性的方法。该方法涉及在受试者的可兴奋细胞中引起甘氨酸受体（GlyR）蛋白质的外源表达。此后，将可兴奋细胞暴露于GlyR蛋白质的变构调节剂。响应于变构调节因子调节外源性GlyR蛋白（离子通道）调节可兴奋细胞的电生理活性。该方法可用于控制受试者的疼痛。本发明还提供了包含编码GlyR蛋白的表达盒，含有这些载体的原料和药物组合物和转基因动物的复制缺陷型HSV载体。

6. 发明授权

US5998174A Multigene vectors 失效
标题翻译：多基因载体
公开(公告)号：US5998174A
公开(公告)日：1999-12-07
申请号：US854601
申请日：1997-05-12
申请人： Joseph C. Glorioso , David Krisky
发明人： Joseph C. Glorioso , David Krisky
IPC分类号： C12N15/38 , C12N15/869 , C12N15/64 , C12N15/31 , C12N15/63
CPC分类号： C12N15/86 , C12N2710/16643
摘要： The present invention provides a method for preparing HSV vectors. The method comprises co-transfecting a source vector and a mutating cassette together into a population of appropriate host cells, such that homologous recombination occurs between the mutating cassette and the source vector whereby the mutating cassette replaces a region of the HSV genome. The mutating cassette has a unique restriction site not present in the sequence of the vector. The method further comprises plaquing the co-transfected host cells, selecting plaques in which recombination has occurred between the source vector and the mutating cassette, and isolating the viral DNA from the plaques. The isolated viral DNA is digested with a restriction endonuclease appropriate for cleaving the viral DNA at the unique restriction site within the mutating cassette to produce two viral polynucleotides. Following purification, the two viral polynucleotides can be ligated to form an HSV vector comprising the two viral polynucleotides. Alternatively, the two isolated viral polynucleotides can be recombined with an insertion cassette to form an HSV vector comprising the insertion cassette at the former locus of the unique restriction site. The present invention further provides a mutant vector, particularly an HSV vector constructed in accordance with the method for the present invention. The present invention further provides a multigene HSV vector, particularly a multigene HSV vector for cancer therapy.
摘要翻译：本发明提供一种HSV载体的制备方法。该方法包括将源载体和突变盒共同转染到合适的宿主细胞群体中，使得在突变盒和源载体之间发生同源重组，由此突变盒替代HSV基因组的区域。突变盒具有不存在于载体序列中的唯一限制性位点。该方法还包括将共转染的宿主细胞进行筛选，选择在源载体和突变盒之间已经发生重组的斑块，并从噬斑中分离病毒DNA。分离的病毒DNA用限制性内切核酸酶消化，其适用于在突变盒内的独特的限制性位点切割病毒DNA，以产生两个病毒多核苷酸。纯化后，可以连接两种病毒多核苷酸以形成包含两种病毒多核苷酸的HSV载体。或者，两个分离的病毒多核苷酸可以与插入盒重组以形成包含在唯一限制性位点前一位点的插入盒的HSV载体。本发明还提供了突变载体，特别是根据本发明的方法构建的HSV载体。本发明还提供了多基因HSV载体，特别是用于癌症治疗的多基因HSV载体。

7. 发明授权

US5858355A IRAP gene as treatment for arthritis 失效
标题翻译： IRAP基因作为关节炎的治疗
公开(公告)号：US5858355A
公开(公告)日：1999-01-12
申请号：US381603
申请日：1995-01-27
申请人： Joseph C. Glorioso , Christopher H. Evans , Paul D. Robbins
发明人： Joseph C. Glorioso , Christopher H. Evans , Paul D. Robbins
IPC分类号： A01K67/027 , A61K9/127 , A61K35/35 , A61K35/76 , A61K38/00 , A61K38/17 , A61K47/30 , A61K48/00 , A61K49/00 , A61P29/00 , C07K14/54 , C07K14/545 , C07K14/715 , C12N5/077 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/85 , C12N15/867 , C12R1/92
CPC分类号： A01K67/0271 , A61K35/35 , A61K48/00 , A61K49/0008 , C07K14/54 , C07K14/545 , C07K14/715 , C12N15/8509 , C12N15/86 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2267/025 , A01K2267/03 , A61K38/00 , C12N2740/13043 , C12N2740/13045 , C12N2750/14143 , C12N2799/025 , C12N2799/027
摘要： The subject invention concerns a method of introducing at least one gene encoding a product into at least one cell of a connective tissue of a mammalian host for use in treating the mammalian host including employing recombinant techniques to produce a DNA vector molecule which contains the gene encoding for the product and infecting the connective cell of the mammalian host using the DNA vector molecule using the gene coding for the product. A method is provided for introducing at least one gene encoding a product into at least one cell of a connective tissue of a mammalian host employing non-viral means. In a specific example, isolated synovial cells were infected with a retrovirus comprising a DNA sequence encoding IRAP and the transfected cells administered to an arthritic joint. A method to produce an animal model for the study of connective tissue pathology is also disclosed. Additionally, this invention provides a method of using in vivo a gene encoding and extracellular interleukin-1 binding domain of an interleukin-1 receptor.
摘要翻译：本发明涉及将编码产物的至少一种基因导入哺乳动物宿主的结缔组织的至少一个细胞以用于治疗哺乳动物宿主的方法，包括使用重组技术来产生含有编码并使用编码产物的基因使用DNA载体分子感染哺乳动物宿主的结缔组织细胞。提供了一种方法，用于使用非病毒方法将至少一种编码产物的基因导入哺乳动物宿主的结缔组织的至少一个细胞。在具体实例中，用包含编码IRAP的DNA序列和施用于关节炎关节的转染细胞的逆转录病毒感染孤立的滑膜细胞。还公开了一种用于产生结缔组织病理学研究的动物模型的方法。另外，本发明提供了在体内使用编码白细胞介素-1受体的细胞外白细胞介素-1结合结构域的方法。

8. 发明授权

US07037492B2 Gene transfer for studying and treating a connective tissue of a mammalian host 失效
公开(公告)号：US07037492B2
公开(公告)日：2006-05-02
申请号：US09731175
申请日：2000-12-05
申请人： Joseph C. Glorioso , Christopher H. Evans , Paul D. Robbins
发明人： Joseph C. Glorioso , Christopher H. Evans , Paul D. Robbins
IPC分类号： A61K35/00 , C12N5/00 , C12N15/63 , C12N15/09
CPC分类号： A01K67/0271 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2267/025 , A01K2267/03 , A61K38/00 , A61K38/162 , A61K48/00 , A61K48/005 , A61K49/0008 , C07K14/54 , C07K14/545 , C07K14/715 , C12N15/8509 , C12N15/86 , C12N2710/10343 , C12N2710/16643 , C12N2740/13043 , C12N2740/13045 , C12N2750/14143 , C12N2799/025 , C12N2799/027
摘要： Methods for introducing at least one gene encoding a product into at least one target cell of a mammalian host for use in treating the mammalian host are disclosed. These methods include employing recombinant techniques to produce a vector molecule that contains the gene encoding for the product, and infecting the target cells of the mammalian host using the DNA vector molecule. A method to produce an animal model for the study of connective tissue pathology is also disclosed.

9. 发明授权

US06881825B1 Identication of peptides that facilitate uptake and cytoplasmic and/or nuclear transport of proteins, DNA and virues 有权
标题翻译：鉴定促进蛋白质，DNA和病毒的摄取和细胞质和/或核转运的肽
公开(公告)号：US06881825B1
公开(公告)日：2005-04-19
申请号：US09653182
申请日：2000-08-31
申请人： Paul D. Robbins , Zhibao Mi , Raymond Frizzell , Joseph C. Glorioso , Andrea Gambotto
发明人： Paul D. Robbins , Zhibao Mi , Raymond Frizzell , Joseph C. Glorioso , Andrea Gambotto
IPC分类号： A61K38/00 , A61K39/00 , A61K47/48 , A61K48/00 , C07K7/06 , C07K7/08 , C12N15/10 , C40B40/02 , A61K38/04 , C12P21/08
CPC分类号： C40B40/02 , A61K38/00 , A61K39/00 , A61K47/62 , A61K48/00 , C07K7/06 , C07K7/08 , C07K2319/00 , C07K2319/10 , C12N15/1037 , G01N33/6842 , G01N2500/10
摘要： The present invention relates to internalizing peptides which facilitate the uptake and transport of cargo into the cytoplasm and nuclei of cells as well as methods for the identification of the peptides, and methods of use for the peptides. The internalizing peptides of the present invention are selected for their ability to efficiently internalize cargo into a wide variety of cell types both in vivo and in vitro.
摘要翻译：本发明涉及促进载体进入细胞的细胞质和细胞核以及鉴定肽的方法和肽的使用方法的内肽化肽。选择本发明的内化肽的能力是在体内和体外有效地将货物内部化成多种细胞类型。

10. 发明授权

US6159464A Viral vectors to inhibit leukocyte infiltration or cartilage degradation of joints 失效
标题翻译：病毒载体抑制白细胞浸润或关节软骨退化
公开(公告)号：US6159464A
公开(公告)日：2000-12-12
申请号：US924376
申请日：1997-09-05
申请人： Joseph C. Glorioso , Christopher H. Evans , Paul D. Robbins , Steven C. Ghivizzani
发明人： Joseph C. Glorioso , Christopher H. Evans , Paul D. Robbins , Steven C. Ghivizzani
IPC分类号： A01N63/00 , A61K48/00 , C07H21/04
CPC分类号： A01K67/0271 , A61K48/005 , C07K14/545 , C07K14/715 , C12N15/8509 , C12N15/86 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/107 , A01K2267/01 , A01K2267/03 , A61K38/00 , A61K48/00 , C12N2740/13043 , C12N2740/13045 , Y10S514/825
摘要： Methods for treating a connective tissue disorder by introducing at least one gene encoding a product into at least one target cell of a mammalian host for use in treating the mammalian host are disclosed. These methods include employing recombinant techniques to produce a vector molecule containing the DNA sequence encoding for the product and infecting the target cell of the mammalian host using the vector. The injection can be done in vivo, by directly injecting the vector into the host, or can be done in vitro by transfecting a population of cultured target cells with the vector and transplanting them each into the host. Nonviral means can also be used to introduce the DNA sequence to the host. Administration of more than one gene of interest results in an enhanced therapeutic benefit. Also disclosed is a method for treating a connective tissue disorder by introducing at least one gene encoding a product into at least one target cell of a joint of a host for use in treating multiple joints of the host. Injection of a vector molecule containing the DNA sequence encoding for a product of interest, or non-viral introduction of such a DNA sequence, to one join of a mammalian host results in a therapeutic benefit in that joint as well as other joints in the host
摘要翻译：公开了通过将编码产物的至少一个基因导入哺乳动物宿主的至少一个靶细胞用于治疗哺乳动物宿主来治疗结缔组织病的方法。这些方法包括使用重组技术来产生含有编码产物的DNA序列并使用载体感染哺乳动物宿主的靶细胞的载体分子。注射可以在体内进行，通过将载体直接注射到宿主中，或者可以通过用载体转染培养的靶细胞群体并将其移植到宿主体内来完成。也可以使用非病毒方法将DNA序列引入宿主。管理多个感兴趣的基因导致增强的治疗益处。还公开了一种通过将至少一种编码产物的基因引入到用于治疗宿主的多个关节的宿主的关节的至少一个靶细胞中来治疗结缔组织病的方法。将含有编码感兴趣的产物的DNA序列或非病毒引入这样的DNA序列的载体分子注射到哺乳动物宿主的一个连接中导致该接合物以及该宿主中的其它关节的治疗益处

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式