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1. 发明授权

US08846889B2 Peptide biosynthesis and pain therapy 有权
标题翻译：肽生物合成和疼痛治疗
公开(公告)号：US08846889B2
公开(公告)日：2014-09-30
申请号：US12893352
申请日：2010-09-29
申请人： Darren P. Wolfe , Joseph C. Glorioso , David J. Fink
发明人： Darren P. Wolfe , Joseph C. Glorioso , David J. Fink
IPC分类号： C07H21/04 , A61K31/711 , C12N15/12 , C12N15/62 , A61K48/00 , G01N33/68 , C07K14/67 , C07K5/107 , C12N15/86 , G01N33/94 , C07K7/22 , C07K14/695 , C07K14/70
CPC分类号： C07K5/1016 , A61K48/005 , C07K7/22 , C07K14/67 , C07K14/695 , C07K14/70 , C07K2319/50 , C07K2319/60 , C12N15/86 , C12N2710/16643 , G01N33/6893 , G01N33/9486 , G01N2800/2842
摘要： The invention provides an expression cassette comprising a DNA sequence encoding amino acids 1-99 of human preproenkephalin, a DNA sequence encoding a precursor of a carboxy-amidated peptide flanked by dibasic cleavage sites and optionally a DNA sequence encoding a marker protein (such as Enhanced Green Fluorescent Protein (GNP)) all in operable linkage and under control of a promoter. Where the encoded precursor of a carboxy-amidated peptide is an agonist for an opioid receptor, the invention further provides a method of treating neuropathic pain by administering the gene transfer vector comprising such an expression cassette to a patient. The invention also provides a method for detecting a peptide having a desired effect comprising introducing a library of DNA sequences encoding one or more precursors of carboxy-amidated peptides into host cells; expressing the carboxy-amidated peptides encoded in the library to provide expression products; and screening from the polypeptide expression products for the desired effect.
摘要翻译：本发明提供了一种表达盒，其包含编码人前脑啡肽的氨基酸1-99的DNA序列，编码侧翼为二碱基切割位点的羧基酰胺化肽的前体的DNA序列和任选的编码标记蛋白的DNA序列（例如增强型绿色荧光蛋白（GNP））均可操作地连接并在启动子的控制下。当羧基酰胺化肽的编码前体是阿片受体的激动剂时，本发明还提供了通过将包含这种表达盒的基因转移载体给予患者来治疗神经性疼痛的方法。本发明还提供了一种检测具有所需效果的肽的方法，包括将编码羧基酰胺化肽的一种或多种前体的DNA序列文库导入宿主细胞; 表达在文库中编码的羧基酰胺化肽以提供表达产物; 并从多肽表达产物筛选出所需的效果。

2. 发明授权

US07825231B2 Method of amidated peptide biosynthesis and delivery in vivo: endomorphin-2 for pain therapy 有权
标题翻译：酰胺化肽合成和体内递送的方法：内啡肽-2用于疼痛治疗
公开(公告)号：US07825231B2
公开(公告)日：2010-11-02
申请号：US11445837
申请日：2006-06-01
申请人： Darren P. Wolfe , Joseph C. Glorioso , David J. Fink
发明人： Darren P. Wolfe , Joseph C. Glorioso , David J. Fink
IPC分类号： C07H21/04 , A61K31/711 , C12N15/12 , C12N15/62
CPC分类号： C07K5/1016 , A61K48/005 , C07K7/22 , C07K14/67 , C07K14/695 , C07K14/70 , C07K2319/50 , C07K2319/60 , C12N15/86 , C12N2710/16643 , G01N33/6893 , G01N33/9486 , G01N2800/2842
摘要： The invention provides an expression cassette comprising a DNA sequence encoding amino acids 1-99 of human preproenkephalin, a DNA sequence encoding a precursor of a carboxy-amidated peptide flanked by dibasic cleavage sites and optionally a DNA sequence encoding a marker protein (such as Enhanced Green Fluorescent Protein (GFP)) all in operable linkage and under control of a promoter. Where the encoded precursor of a carboxy-amidated peptide is an agonist for an opioid receptor, the invention further provides a method of treating neuropathic pain by administering the gene transfer vector comprising such an expression cassette to a patient. The invention also provides a method for detecting a peptide having a desired effect comprising introducing a library of DNA sequences encoding one or more precursors of carboxy-amidated peptides into host cells; expressing the carboxy-amidated peptides encoded in the library to provide expression products; and screening from the polypeptide expression products for the desired effect.
摘要翻译：本发明提供了一种表达盒，其包含编码人前脑啡肽的氨基酸1-99的DNA序列，编码侧翼为二碱基切割位点的羧基酰胺化肽的前体的DNA序列和任选的编码标记蛋白的DNA序列（例如增强型绿色荧光蛋白（GFP））都在可操作的连锁和启动子的控制下。当羧基酰胺化肽的编码前体是阿片受体的激动剂时，本发明还提供了通过将包含这种表达盒的基因转移载体给予患者来治疗神经性疼痛的方法。本发明还提供了一种检测具有所需效果的肽的方法，包括将编码羧基酰胺化肽的一种或多种前体的DNA序列文库导入宿主细胞; 表达在文库中编码的羧基酰胺化肽以提供表达产物; 并从多肽表达产物筛选出所需的效果。

3. 发明授权

US08003622B2 Peptide biosynthesis and pain therapy 有权
标题翻译：肽生物合成和疼痛治疗
公开(公告)号：US08003622B2
公开(公告)日：2011-08-23
申请号：US12893255
申请日：2010-09-29
申请人： Darren P. Wolfe , Joseph C. Glorioso , David J. Fink
发明人： Darren P. Wolfe , Joseph C. Glorioso , David J. Fink
IPC分类号： A61K48/00 , C12N15/00
CPC分类号： C07K5/1016 , A61K48/005 , C07K7/22 , C07K14/67 , C07K14/695 , C07K14/70 , C07K2319/50 , C07K2319/60 , C12N15/86 , C12N2710/16643 , G01N33/6893 , G01N33/9486 , G01N2800/2842
摘要： The invention provides an expression cassette comprising a DNA sequence encoding amino acids 1-99 of human preproenkephalin, a DNA sequence encoding a precursor of a carboxy-amidated peptide flanked by dibasic cleavage sites and optionally a DNA sequence encoding a marker protein (such as Enhanced Green Fluorescent Protein (GFP)) all in operable linkage and under control of a promoter. Where the encoded precursor of a carboxy-amidated peptide is an agonist for an opioid receptor, the invention further provides a method of treating neuropathic pain by administering the gene transfer vector comprising such an expression cassette to a patient. The invention also provides a method for detecting a peptide having a desired effect comprising introducing a library of DNA sequences encoding one or more precursors of carboxy-amidated peptides into host cells; expressing the carboxy-amidated peptides encoded in the library to provide expression products; and screening from the polypeptide expression products for the desired effect.
摘要翻译：本发明提供了一种表达盒，其包含编码人前脑啡肽的氨基酸1-99的DNA序列，编码侧翼为二碱基切割位点的羧基酰胺化肽的前体的DNA序列和任选的编码标记蛋白的DNA序列（例如增强型绿色荧光蛋白（GFP））都在可操作的连锁和启动子的控制下。当羧基酰胺化肽的编码前体是阿片受体的激动剂时，本发明还提供了通过将包含这种表达盒的基因转移载体给予患者来治疗神经性疼痛的方法。本发明还提供了一种检测具有所需效果的肽的方法，包括将编码羧基酰胺化肽的一种或多种前体的DNA序列文库导入宿主细胞; 表达在文库中编码的羧基酰胺化肽以提供表达产物; 并从多肽表达产物筛选出所需的效果。

4. 发明申请

US20110112175A1 PEPTIDE BIOSYNTHESIS AND PAIN THERAPY 有权
标题翻译：肽生物合成和治疗
公开(公告)号：US20110112175A1
公开(公告)日：2011-05-12
申请号：US12893352
申请日：2010-09-29
申请人： DARREN P. WOLFE , JOSEPH C. GLORIOSO , DAVID J. FINK
发明人： DARREN P. WOLFE , JOSEPH C. GLORIOSO , DAVID J. FINK
IPC分类号： A61K31/7088 , C07H21/00 , C40B40/08 , C12N15/63 , A61P29/00 , A61P25/04
CPC分类号： C07K5/1016 , A61K48/005 , C07K7/22 , C07K14/67 , C07K14/695 , C07K14/70 , C07K2319/50 , C07K2319/60 , C12N15/86 , C12N2710/16643 , G01N33/6893 , G01N33/9486 , G01N2800/2842
摘要： The invention provides an expression cassette comprising a DNA sequence encoding amino acids 1-99 of human preproenkephalin, a DNA sequence encoding a precursor of a carboxy-amidated peptide flanked by dibasic cleavage sites and optionally a DNA sequence encoding a marker protein (such as Enhanced Green Fluorescent Protein (GNP)) all in operable linkage and under control of a promoter. Where the encoded precursor of a carboxy-amidated peptide is an agonist for an opioid receptor, the invention further provides a method of treating neuropathic pain by administering the gene transfer vector comprising such an expression cassette to a patient. The invention also provides a method for detecting a peptide having a desired effect comprising introducing a library of DNA sequences encoding one or more precursors of carboxy-amidated peptides into host cells; expressing the carboxy-amidated peptides encoded in the library to provide expression products; and screening from the polypeptide expression products for the desired effect.
摘要翻译：本发明提供了一种表达盒，其包含编码人前脑啡肽的氨基酸1-99的DNA序列，编码侧翼为二碱基切割位点的羧基酰胺化肽的前体的DNA序列和任选的编码标记蛋白的DNA序列（例如增强型绿色荧光蛋白（GNP））均可操作地连接并在启动子的控制下。当羧基酰胺化肽的编码前体是阿片受体的激动剂时，本发明还提供了通过将包含这种表达盒的基因转移载体给予患者来治疗神经性疼痛的方法。本发明还提供了一种检测具有所需效果的肽的方法，包括将编码羧基酰胺化肽的一种或多种前体的DNA序列文库导入宿主细胞; 表达在文库中编码的羧基酰胺化肽以提供表达产物; 并从多肽表达产物筛选出所需的效果。

5. 发明申请

US20110112174A1 PEPTIDE BIOSYNTHESIS AND PAIN THERAPY 有权
标题翻译：肽类生物化学和药物治疗
公开(公告)号：US20110112174A1
公开(公告)日：2011-05-12
申请号：US12893255
申请日：2010-09-29
申请人： Darren P. Wolfe , Joseph C. Glorioso , David J. Fink
发明人： Darren P. Wolfe , Joseph C. Glorioso , David J. Fink
IPC分类号： A61K31/711 , A61P35/00 , A61P29/00 , A61P25/00 , A61P25/04
CPC分类号： C07K5/1016 , A61K48/005 , C07K7/22 , C07K14/67 , C07K14/695 , C07K14/70 , C07K2319/50 , C07K2319/60 , C12N15/86 , C12N2710/16643 , G01N33/6893 , G01N33/9486 , G01N2800/2842
摘要： The invention provides an expression cassette comprising a DNA sequence encoding amino acids 1-99 of human preproenkephalin, a DNA sequence encoding a precursor of a carboxy-amidated peptide flanked by dibasic cleavage sites and optionally a DNA sequence encoding a marker protein (such as Enhanced Green Fluorescent Protein (GFP)) all in operable linkage and under control of a promoter. Where the encoded precursor of a carboxy-amidated peptide is an agonist for an opioid receptor, the invention further provides a method of treating neuropathic pain by administering the gene transfer vector comprising such an expression cassette to a patient. The invention also provides a method for detecting a peptide having a desired effect comprising introducing a library of DNA sequences encoding one or more precursors of carboxy-amidated peptides into host cells; expressing the carboxy-amidated peptides encoded in the library to provide expression products; and screening from the polypeptide expression products for the desired effect.
摘要翻译：本发明提供了一种表达盒，其包含编码人前脑啡肽的氨基酸1-99的DNA序列，编码侧翼为二碱基切割位点的羧基酰胺化肽的前体的DNA序列和任选的编码标记蛋白的DNA序列（例如增强型绿色荧光蛋白（GFP））都在可操作的连锁和启动子的控制下。当羧基酰胺化肽的编码前体是阿片受体的激动剂时，本发明还提供了通过将包含这种表达盒的基因转移载体给予患者来治疗神经性疼痛的方法。本发明还提供了一种检测具有所需效果的肽的方法，包括将编码羧基酰胺化肽的一种或多种前体的DNA序列文库导入宿主细胞; 表达在文库中编码的羧基酰胺化肽以提供表达产物; 并从多肽表达产物筛选出所需的效果。

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