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1. 发明申请

US20090311670A1 APPARATUS AND METHOD FOR MEASURING CONCENTRATION OF MOLECULES THROUGH A BARRIER 审中-公开
标题翻译：用于测量通过障碍物分子浓度的装置和方法
公开(公告)号：US20090311670A1
公开(公告)日：2009-12-17
申请号：US12544440
申请日：2009-08-20
申请人： Ivan E. Modrovich
发明人： Ivan E. Modrovich
IPC分类号： C12M1/34 , C12Q1/02 , G01N33/53 , C12Q1/70 , C12Q1/54
CPC分类号： A61B5/1486 , A61B5/14532 , A61B5/411
摘要： An apparatus and method for detecting the presence and measuring the concentrations of molecules through a barrier and/or at a distance utilizes the principle of chemical/electrostatic attraction (hereinafter “affinity”), i.e., the affinity between charged atoms and molecules that cause their chemical interactions and reactions, to infer, based on the behavior of molecules on one side of the barrier, the presence and concentration of corresponding molecules on the other side of the barrier. The invention is useful, by way of example and not limitation, in non-invasively measuring glucose levels of diabetic patients.
摘要翻译：用于通过屏障和/或在距离处检测分子的存在和测量的装置和方法利用化学/静电吸引原理（以下称为“亲和性”），即带电原子和分子之间的亲和力，导致它们化学相互作用和反应，基于屏障一侧的分子的行为推断屏障另一侧上相应分子的存在和浓度。本发明以糖尿病患者的非侵入性测量葡萄糖水平为例，而非限制性是有用的。

2. 发明授权

US6143537A Method for stabilizing analyte structure using antibodies or antibody fragments thereof 失效
标题翻译：使用抗体或其抗体片段稳定分析物结构的方法
公开(公告)号：US6143537A
公开(公告)日：2000-11-07
申请号：US225905
申请日：1999-01-05
申请人： Shing F. Kwan , Ivan E. Modrovich , Rebecca J. Hunt
发明人： Shing F. Kwan , Ivan E. Modrovich , Rebecca J. Hunt
IPC分类号： G01N33/531 , A61K39/395 , C07K16/40 , C12N9/96 , G01N33/53 , C12Q1/32 , C12Q1/37 , G01N33/573 , C12Q9/96
CPC分类号： C07K16/40 , C12N9/96 , C07K2317/55 , Y10S436/826 , Y10S524/90 , Y10T436/10 , Y10T436/106664 , Y10T436/108331 , Y10T436/2525
摘要： A method for stabilizing analyses with antibodies and antibody fragments comprises dissolving the analyte in a liquid to form a solution, adding analyte-specific antibodies, fragments of such antibodies, or both to the solution, heating the solution, and then cooling and filtering the solution. The filtered solution may be diluted in a suitable matrix.
摘要翻译：用抗体和抗体片段稳定分析的方法包括将分析物溶解在液体中以形成溶液，将分析物特异性抗体，此类抗体的片段或两者加入到溶液中，加热溶液，然后冷却和过滤溶液。过滤的溶液可以在合适的基质中稀释。

3. 发明授权

US5660978A Stabilization of analytes 失效
标题翻译：分析物的稳定
公开(公告)号：US5660978A
公开(公告)日：1997-08-26
申请号：US483375
申请日：1995-06-07
申请人： Shing Fai Kwan , Ivan E. Modrovich , Rebecca Jolene Hunt
发明人： Shing Fai Kwan , Ivan E. Modrovich , Rebecca Jolene Hunt
IPC分类号： G01N33/531 , A61K39/395 , C07K16/40 , C12N9/96 , C12Q1/70
CPC分类号： C07K16/40 , C12N9/96 , C07K2317/55 , Y10S436/826 , Y10S524/90 , Y10T436/10 , Y10T436/106664 , Y10T436/108331 , Y10T436/2525
摘要： A method for stabilizing analytes with antibodies and antibody fragments comprises dissolving the analyte in a liquid to form a solution, adding analyte-specific antibodies, fragments of such antibodies, or both to the solution, heating the solution, and then cooling and filtering the solution. The filtered solution may be diluted in a suitable matrix.
摘要翻译：用抗体和抗体片段稳定分析物的方法包括将分析物溶解在液体中以形成溶液，将分析物特异性抗体，此类抗体片段或两者加入溶液中，加热溶液，然后冷却和过滤溶液。过滤的溶液可以在合适的基质中稀释。

4. 发明授权

US4465770A Stabilized enzymic solutions for determining urea 失效
标题翻译：用于测定尿素的稳定酶溶液
公开(公告)号：US4465770A
公开(公告)日：1984-08-14
申请号：US287907
申请日：1981-07-29
申请人： Ivan E. Modrovich
发明人： Ivan E. Modrovich
IPC分类号： C12N9/80 , C12N9/96 , C12Q1/58
CPC分类号： C12N9/80 , C12N9/96 , C12Q1/58 , Y10S435/81 , Y10T436/103332
摘要： A stabilized urease solution is disclosed comprising water, buffering agents, a bacteriostat, chelating agent, polyhydroxy organic compound and urease. The preferred buffering agents are selected from low conductivity Zwitterionic buffer salts, such as N-2-hydroxyethyl piperazine-N'-2-ethanesulfonic acid, triethanolamine, tris(hydroxymethyl)aminomethane, diethanolamine, aminomethyl propanol and mixtures thereof.
摘要翻译：公开了稳定的脲酶溶液，其包含水，缓冲剂，抑菌剂，螯合剂，多羟基有机化合物和脲酶。优选的缓冲剂选自低电导率两性离子缓冲盐，例如N-2-羟乙基哌嗪-N'-2-乙磺酸，三乙醇胺，三（羟甲基）氨基甲烷，二乙醇胺，氨基甲基丙醇及其混合物。

5. 发明授权

US4271264A Stabilized liquid enzyme and coenzyme compositions 失效
标题翻译：稳定液体酶和辅酶组合物
公开(公告)号：US4271264A
公开(公告)日：1981-06-02
申请号：US862106
申请日：1977-12-19
申请人： Ivan E. Modrovich
发明人： Ivan E. Modrovich
IPC分类号： C12N9/96 , C12Q1/00 , C12Q1/54
CPC分类号： C12Q1/008 , C12N9/96 , C12Q1/54
摘要： Stabilized liquid enzyme and coenzyme compositions are prepared for use in biological diagnostic determination of glucose. In one embodiment a composition is prepared containing in admixture an aqueous vehicle, a nicotinamide-adenine dinucleotide coenzyme, a nucleotide, an enzyme including hexokinase and/or glucose-6-phosphate dehydrogenase and an organic solvent to stabilize the enzyme, coenzyme and nucleotide. The compositions have a pH of 6.0 to 8.5 and may contain a bacteriostat such as an azide compound. A preferred organic solvent is a polyol. In an alternative embodiment, a two reagent liquid enzyme and coenzyme composition is prepared wherein the enzyme is stabilized in one solution and the coenzyme and nucleotide stabilized in another solution, and the two solutions combined when used. In the enzyme-containing solution, an ammonia salt can be used as an alternative to the organic solvent to stabilize the enzyme. The compositions exhibit excellent shelf life and the container in which the compositions are stored can be repeatedly opened for use without any substantial degradation of the enzyme, coenzyme and nucleotide.
摘要翻译：制备稳定液体酶和辅酶组合物用于葡萄糖的生物诊断测定。在一个实施方案中，制备组合物，其混合含有载体，烟酰胺 - 腺嘌呤二核苷酸辅酶，核苷酸，包含己糖激酶和/或葡萄糖-6-磷酸脱氢酶的酶和有机溶剂，以稳定酶，辅酶和核苷酸。组合物的pH为6.0-8.5，并可含有抑菌剂，例如叠氮化合物。优选的有机溶剂是多元醇。在替代实施方案中，制备两种试剂液体酶和辅酶组合物，其中酶在一种溶液中稳定，并且辅酶和核苷酸在另一种溶液中稳定，并且两种溶液在使用时相结合。在含酶溶液中，氨盐可用作有机溶剂的替代物以稳定酶。组合物表现出优异的保存期限，并且其中储存组合物的容器可以反复打开以使用，而不会显着降解酶，辅酶和核苷酸。

6. 发明授权

US4409326A Stabilized enzymatic solutions and method for determining total cholesterol in human serum 失效
标题翻译：稳定的酶溶液和测定人血清中总胆固醇的方法
公开(公告)号：US4409326A
公开(公告)日：1983-10-11
申请号：US308162
申请日：1981-10-02
申请人： Ivan E. Modrovich
发明人： Ivan E. Modrovich
IPC分类号： C12N9/96 , C12Q1/60 , C12Q1/26 , C12Q1/28
CPC分类号： C12N9/96 , C12Q1/60 , Y10S435/81
摘要： Stabilized enzymes useful in the diagnostic assay of total cholesterol are prepared by combining three separately prepared reagents: (1) a cholesterol esterase reagent; (2) cholesterol oxidase reagent; and (3) a saline diluent reagent. The cholesterol esterase reagent is prepared by dissolving a salt of cholic acid in a buffer solution providing a pH within the range of about 4 to about 9. To this solution is added a cholesterol esterase. The solution is then mixed with a polyhydroxy organic compound and TRITON-X-100. A cholesterol oxidase and a polyhydroxy organic compound are each dissolved in separate portions of buffer solution to make the cholesterol esterase reagent. The saline diluent solution is prepared by dissolving potassium iodide in water and adding a polyhydroxy organic compound. The resultant three reagents, when combined, provide a stabilized enzyme solution having utility in the total cholesterol assay of a serum sample. The combined reagents have utility in a total oxygen consumption method of analysis of total cholesterol, which analysis method is also disclosed.
摘要翻译：通过组合三种单独制备的试剂制备可用于总胆固醇诊断测定的稳定酶：（1）胆固醇酯酶试剂; （2）胆固醇氧化酶试剂; 和（3）盐水稀释剂。通过将胆酸盐溶解在提供pH在约4至约9范围内的缓冲溶液中来制备胆固醇酯酶试剂。向该溶液中加入胆固醇酯酶。然后将溶液与多羟基有机化合物和TRITON-X-100混合。将胆固醇氧化酶和多羟基有机化合物各自溶解在缓冲溶液的分开部分中以制备胆固醇酯酶试剂。通过将碘化钾溶解在水中并加入多羟基有机化合物来制备盐水稀释溶液。所得到的三种试剂当组合时提供在血清样品的总胆固醇测定中具有效用的稳定的酶溶液。组合试剂在总胆固醇分析总耗氧方法中具有实用价值，同时也披露了该方法。

7. 发明授权

US4394449A Stabilization of coenzymes in aqueous solution 失效
标题翻译：在水溶液中稳定辅酶
公开(公告)号：US4394449A
公开(公告)日：1983-07-19
申请号：US121225
申请日：1980-02-13
申请人： Ivan E. Modrovich
发明人： Ivan E. Modrovich
IPC分类号： C12N9/96 , C12P1/00 , C12Q1/00 , C12Q1/48 , C12Q1/58 , C12Q1/34 , C12Q1/50
CPC分类号： C12Q1/58 , C12P1/00 , C12Q1/008 , C12Q1/48
摘要： A stabilized aqueous coenzyme solution is disclosed for use in the clinical assay of a selected biological constituent through the enzymic reaction of an enzyme with a selective substrate for the enzyme and a coenzyme. The aqueous coenzyme solution comprises at least one first enzyme and a first substrate for selectively reacting with the first enzyme in the assay, a coenzyme for interacting with the first enzyme and first substrate in the assay which coenzyme degrades forming a coenzyme conversion product, and at least one second enzyme and second substrate selective for the second enzyme which react with the coenzyme conversion product to regenerate the coenzyme.
摘要翻译：公开了一种稳定的含水辅酶溶液，用于通过酶与酶和辅酶的选择性底物的酶反应来选择的生物成分的临床测定。含水辅酶溶液包含至少一种第一酶和第一底物，用于在测定中选择性地与第一种酶反应，辅酶在分析中与第一种酶和第一种底物相互作用，其中辅酶降解形成辅酶转化产物，并且在对于与辅酶转化产物反应以再生辅酶的第二种酶选择性的至少一秒的酶和第二底物。

8. 发明授权

US4378430A Method of forming stabilized urease solutions 失效
标题翻译：形成稳定的脲酶溶液的方法
公开(公告)号：US4378430A
公开(公告)日：1983-03-29
申请号：US215622
申请日：1980-12-12
申请人： Ivan E. Modrovich
发明人： Ivan E. Modrovich
IPC分类号： C12N9/80 , C12N9/96 , C12Q1/58
CPC分类号： C12N9/80 , C12N9/96 , C12Q1/58
摘要： A stabilized urease solution is formulated by blending water, buffering agents, a bacteriostat, chelating agent, polyhydroxy organic compound and urease. The preferred buffering agents are selected from low-conductivity zwitterionic buffer salts, such as N-2-hydroxyethyl-piperazine-N'-2-ethanesulfonic acid, triethanolamine, tris(hydroxymethyl)aminomethane, diethanolamine, aminomethyl propanol and mixtures thereof.
摘要翻译：稳定的脲酶溶液通过混合水，缓冲剂，抑菌剂，螯合剂，多羟基有机化合物和脲酶来配制。优选的缓冲剂选自低电导率两性离子缓冲盐，例如N-2-羟乙基 - 哌嗪-N'-2-乙磺酸，三乙醇胺，三（羟甲基）氨基甲烷，二乙醇胺，氨基甲基丙醇及其混合物。

9. 发明授权

US4310625A Stabilized liquid enzyme compositions for diagnostic determinations 失效
标题翻译：用于诊断测定的稳定液体酶组合物
公开(公告)号：US4310625A
公开(公告)日：1982-01-12
申请号：US898704
申请日：1978-04-24
申请人： Ivan E. Modrovich
发明人： Ivan E. Modrovich
IPC分类号： C12N9/96 , C12Q1/00 , C12Q1/48 , C12Q1/32 , C12Q1/50 , C12Q1/52
CPC分类号： C12Q1/48 , C12N9/96 , C12Q1/008
摘要： Labile enzymes for use in biological diagnostic determinations are stabilized for long terms by forming a solution of the enzyme in an aqueous media containing at least 20% organic solvent such as 30% aqueous propane diol in the presence of a small amount of polymer such as 0.1% gelatin and then diluting the media at least 20 times with water while maintaining the polymer concentration at least 0.01%. The resultant liquid enzyme composition can be stored for extended periods without loss of significant enzyme catalytic activity. Stability is further enhanced by including from 1 to 18% of salts and 0.1% bacteriastatic agents in the liquid enzyme composition. The liquid enzyme composition enzyme can further contain an enzyme substrate and buffer salts.
摘要翻译：用于生物诊断测定的不稳定酶通过在少量聚合物例如0.1的存在下，在含有至少20％有机溶剂如30％丙二醇水溶液的水性介质中形成酶的溶液而长期稳定％明胶，然后用水稀释介质至少20次，同时保持聚合物浓度至少为0.01％。所得液态酶组合物可以长时间储存，而不会显着的酶催化活性丧失。通过在液体酶组合物中含有1至18％的盐和0.1％的抑菌剂来进一步提高稳定性。液体酶组合物酶可进一步含有酶底物和缓冲盐。

10. 发明授权

US5910422A Stable single liquid alpha-amylase reagent 失效
标题翻译：稳定的单液体α-淀粉酶试剂
公开(公告)号：US5910422A
公开(公告)日：1999-06-08
申请号：US700996
申请日：1991-05-10
申请人： Ivan E. Modrovich , Shing S. Kwan , Leon C. Wortham
发明人： Ivan E. Modrovich , Shing S. Kwan , Leon C. Wortham
IPC分类号： C12Q1/34 , C12Q1/40
CPC分类号： C12Q1/40 , C12Q2334/10
摘要： A stable, single liquid alpha-amylase assay reagent comprises a polysaccharide or long chain oligosaccharide substrate for alpha-amylase having an optically detectable label bonded to the reducing end glucose, by a bond cleavable by alpha- or beta-glucosidase, and a blocking group bonded to the terminal end glucose. The reagent further comprises beta-amylase and either alpha- or beta-glucosidase. Sorbitol is provided in an amount of about 5% to retard degradation of the enzymes. Zwitterionic buffers are also provided to stabilize the enzymes. In preparing the reagent, the enzymes and substrate are filtered through a 0.2 micron or less filter to remove alpha-amylase producing organisms.
摘要翻译：稳定的单液体α-淀粉酶测定试剂包含通过α-或β-葡糖苷酶可切割的键和具有与α-还原末端葡萄糖结合的可光学检测标记的α-淀粉酶的多糖或长链寡糖底物和封闭基团与末端葡萄糖结合。试剂还包含β-淀粉酶和α-葡萄糖苷或β-葡糖苷酶。提供山梨糖醇的量为约5％以阻止酶的降解。还提供两性离子缓冲液以稳定酶。在制备试剂时，酶和底物通过0.2微米或更少的过滤器过滤以除去产生α-淀粉酶的生物体。

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