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1. 发明授权

US4950597A Modification of cellulose normally synthesizied by cellulose-producing microorganisms 失效
标题翻译：纤维素生产微生物通常合成的纤维素的改性
公开(公告)号：US4950597A
公开(公告)日：1990-08-21
申请号：US198784
申请日：1988-05-25
申请人： Inder M. Saxena , Eric M. Roberts , R. Malcolm Brown, Jr.
发明人： Inder M. Saxena , Eric M. Roberts , R. Malcolm Brown, Jr.
IPC分类号： C12P19/04
CPC分类号： C12R1/02 , C12P19/04 , Y10S435/822 , Y10S435/823
摘要： The present invention involves a process for screening for and isolating spontaneously occurring or induced cellulose II-producing microorganisms. The process comprises a series of steps in various embodiments. Initially, cellulose-producing microorganisms from a first culture are plated out on a nutrient agar plate. The nutrient agar plate is then incubated to facilitate formation of colonies from single microorganisms. Samples of liquid nutrient medium are then inoculated with microorganisms from colonies having a smooth configuration, as compared to the usual rough colony configuration. The inoculated samples are then aerobically incubated to facilitate microorganism proliferation and pellicle formation. From these incubated samples are selected microorganisms, which, after a cultivation period, have proliferated but not formed a pellicle. Said selected microorganisms produce cellulose II instead of the cellulose I produced by pellicle-forming organisms.
摘要翻译：本发明涉及用于筛选和分离自发产生或诱导的产生纤维素II的微生物的方法。该方法包括各种实施方案中的一系列步骤。最初，将来自第一培养物的产生纤维素的微生物铺在营养琼脂平板上。然后培养营养琼脂平板以促进从单一微生物形成菌落。与通常的粗殖群配置相比，液体营养培养基的样品接种具有平滑构型的菌落的微生物。然后将接种的样品有氧培养以促进微生物增殖和防护薄膜组织。从这些孵育的样品中选出微生物，其在培养期后已增殖但未形成防护薄膜。所述选择的微生物产生纤维素II，而不是由防护薄膜组织生物体产生的纤维素I。

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