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1. 发明申请

US20090171051A1 Method For Producing Amide Compound 审中-公开
标题翻译：生产酰胺化合物的方法
公开(公告)号：US20090171051A1
公开(公告)日：2009-07-02
申请号：US12089511
申请日：2006-10-06
申请人： Hiroko Shibamoto , Toshikazu Aikawa , Teruo Arii , Masanori Muramoto , Takeshi Fukuda , Kiyoshi Ito , Takeya Abe , Souichi Hazama
发明人： Hiroko Shibamoto , Toshikazu Aikawa , Teruo Arii , Masanori Muramoto , Takeshi Fukuda , Kiyoshi Ito , Takeya Abe , Souichi Hazama
IPC分类号： C08F22/38 , C12P13/02
CPC分类号： C12P13/00 , C07C231/06 , C08F220/56 , C12P13/02 , C07C233/09
摘要： [Problems] The present invention provides a method for efficiently producing a corresponding amide compound from a nitrile compound by a reaction using a nitrile hydratase and a method for producing an amide-based polymer excellent in quality from the amide compound. In addition, the present invention provides a method for more efficiently producing an acrylamide with higher quality by a microbial catalyst containing a nitrile hydratase and the like and a method for producing an acrylamide-based polymer, which is excellent in hue, has a good balance between the water solubility and the high molecular weight and is also excellent in quality, by using the acrylamide.[Means for Solving the Problems] The method for producing an amide compound of the present invention is characterized in that in a method for producing an amide compound from a nitrile compound in an aqueous medium in the presence of a catalyst having a nitrile hydratase activity, the concentration of benzene in the aqueous medium is 4.0 ppm or less. In addition, the method for producing an amide-based polymer of the present invention is characterized by homopolymerizing the amide compound or by copolymerizing the amide compound and at least unsaturated monomer copolymerizable with the amide compound. Further, the method for producing acrylamide of the present invention is characterized by hydrating acrylonitrile having a concentration of acrolein of 1 ppm or less by a microbial cell containing a nitrile hydratase or a processed product of the microbial cell in an aqueous medium. Furthermore, the method for producing an acrylamide-based polymer of the present invention is characterized by homopolymerizing the acrylamide or by copolymerizing the acrylamide and at least one unsaturated monomer copolymerizable with the acrylamide.
摘要翻译：本发明提供一种通过使用腈水合酶的反应从腈化合物有效地制备相应的酰胺化合物的方法和由酰胺化合物质量优异的酰胺类聚合物的制备方法。此外，本发明提供了一种通过含有腈水合酶等的微生物催化剂更有效地制备丙烯酰胺的方法以及色调优异的丙烯酰胺类聚合物的制造方法，其平衡性良好在水溶性和高分子量之间，并且通过使用丙烯酰胺也具有优异的质量。解决问题的手段本发明的酰胺化合物的制造方法的特征在于，在具有腈水合酶活性的催化剂存在下，在含水介质中由腈化合物制备酰胺化合物的方法中，水介质中苯的浓度为4.0ppm以下。此外，本发明的酰胺系聚合物的制造方法的特征在于，使酰胺化合物均聚，或者使酰胺化合物与至少与酰胺化合物可共聚的不饱和单体共聚。此外，本发明的丙烯酰胺的制造方法的特征在于，通过含有腈水合酶的微生物细胞或微生物细胞的加工产物在水性介质中使丙烯醛浓度为1ppm以下的丙烯腈水合。此外，本发明的丙烯酰胺类聚合物的制造方法的特征在于使丙烯酰胺均聚或通过共聚丙烯酰胺和至少一种与丙烯酰胺共聚的不饱和单体。

2. 发明授权

US09150885B2 Method for producing isopropyl alcohol by continuous culture 有权
标题翻译：通过连续培养生产异丙醇的方法
公开(公告)号：US09150885B2
公开(公告)日：2015-10-06
申请号：US14237860
申请日：2012-08-09
申请人： Hiroko Shibamoto
发明人： Hiroko Shibamoto
IPC分类号： C12P7/04
CPC分类号： C12P7/04
摘要： A method of producing isopropyl alcohol includes: culturing an isopropyl alcohol-producing Escherichia coli under a bacterial cell growth condition in which the Escherichia coli stably proliferates in an isopropyl alcohol production period while continuously supplying a substrate solution to a culture tank and continuously removing a product-containing culture solution from the culture tank, the substrate solution containing a plant-derived raw material, the number of cells of the isopropyl alcohol-producing Escherichia coli in the culture tank being maintained during the culturing, and the isopropyl alcohol-producing Escherichia coli having isopropyl alcohol production ability introduced or modified by genetic recombination; bringing the isopropyl-alcohol-producing Escherichia coli into contact with the plant-derived raw material in the culture tank to produce isopropyl alcohol; and recovering the isopropyl alcohol produced by the isopropyl alcohol-producing Escherichia coli from the culture solution that contains the product and that has been removed from the culture tank.
摘要翻译：异丙醇的制造方法包括：在异丙醇生产期间，在大肠杆菌稳定地增殖的细菌细胞生长条件下培养产生异丙醇的大肠杆菌，同时将培养液连续地供给到培养槽中并连续地除去产物含有植物来源的原料的底物溶液，培养槽内维持的异丙醇生产性大肠杆菌的细胞数和产生异丙醇的大肠杆菌通过基因重组引入或修饰异丙醇生产能力; 将产生异丙醇的大肠杆菌与培养罐中的植物来源的原料接触以产生异丙醇; 并从含有产物的培养液中回收由产生异丙醇的大肠杆菌产生的异丙醇，并从培养槽中取出。

3. 发明授权

US08329843B2 Method for producing amide compound 有权
标题翻译：酰胺化合物的制备方法
公开(公告)号：US08329843B2
公开(公告)日：2012-12-11
申请号：US13197066
申请日：2011-08-03
申请人： Hiroko Shibamoto , Toshikazu Aikawa , Teruo Arii , Masanori Muramoto , Takeshi Fukuda , Kiyoshi Ito , Takeya Abe , Souichi Hazama
发明人： Hiroko Shibamoto , Toshikazu Aikawa , Teruo Arii , Masanori Muramoto , Takeshi Fukuda , Kiyoshi Ito , Takeya Abe , Souichi Hazama
IPC分类号： C12P13/00 , C08F20/02
CPC分类号： C12P13/00 , C07C231/06 , C08F220/56 , C12P13/02 , C07C233/09
摘要： A method for producing an amide compound from a nitrile compound in an aqueous medium in the presence of a catalyst having a nitrile hydratase activity wherein the concentration of benzene in the aqueous medium is 4.0 ppm or less and a method for producing an amide-based polymer excellent in quality from the amide compound. Also provided are a method for more efficiently producing an acrylamide with higher quality by a microbial catalyst containing a nitrile hydratase and the like and a method for producing an acrylamide-based polymer, which is excellent in hue, has a good balance between water solubility and high molecular weight and is excellent in quality.
摘要翻译：在具有腈水合酶活性的催化剂存在下，在含水介质中由腈化合物制备酰胺化合物的方法，其中水介质中苯的浓度为4.0ppm或更低，以及制备酰胺基聚合物的方法优异的酰胺化合物。还提供了通过含有腈水合酶等的微生物催化剂更有效地制备具有更高质量的丙烯酰胺的方法和色调优异的丙烯酰胺类聚合物的制造方法，水溶性与高分子量，质量优良。

4. 发明授权

US09096874B2 Method for producing lactic acid under pressure that exceeds normal atmospheric pressure 有权
标题翻译：在超过正常大气压的压力下生产乳酸的方法
公开(公告)号：US09096874B2
公开(公告)日：2015-08-04
申请号：US13387580
申请日：2010-07-28
申请人： Hiroko Shibamoto
发明人： Hiroko Shibamoto
IPC分类号： C12P7/56
CPC分类号： C12P7/56
摘要： Provided is a method for producing lactic acid, which includes: obtaining D-lactic acid or L-lactic acid by carrying out lactic acid fermentation using a lactic acid-producing microorganism under a pressurized condition that exceeds normal pressure and is capable of maintaining lactic acid production activity of the lactic acid-producing microorganism.
摘要翻译：提供一种生产乳酸的方法，其包括：通过在超过常压的加压条件下使用产生乳酸的微生物进行乳酸发酵来获得D-乳酸或L-乳酸，并且能够保持乳酸乳酸生产微生物的生产活性。

5. 发明申请

US20120122167A1 METHOD FOR PRODUCING LACTIC ACID 有权
标题翻译：生产乳酸的方法
公开(公告)号：US20120122167A1
公开(公告)日：2012-05-17
申请号：US13387580
申请日：2010-07-28
申请人： Hiroko Shibamoto
发明人： Hiroko Shibamoto
IPC分类号： C12P7/56
CPC分类号： C12P7/56
摘要： Provided is a method for producing lactic acid, which includes: obtaining D-lactic acid or L-lactic acid by carrying out lactic acid fermentation using a lactic acid-producing microorganism under a pressurized condition that exceeds normal pressure and is capable of maintaining lactic acid production activity of the lactic acid-producing microorganism.
摘要翻译：提供一种生产乳酸的方法，其包括：通过在超过常压的加压条件下使用产生乳酸的微生物进行乳酸发酵来获得D-乳酸或L-乳酸，并且能够保持乳酸乳酸生产微生物的生产活性。

6. 发明申请

US20140199742A1 METHOD FOR PRODUCING ISOPROPYL ALCOHOL BY CONTINUOUS CULTURE 有权
标题翻译：通过连续培养生产异丙醇的方法
公开(公告)号：US20140199742A1
公开(公告)日：2014-07-17
申请号：US14237860
申请日：2012-08-09
申请人： Hiroko Shibamoto
发明人： Hiroko Shibamoto
IPC分类号： C12P7/04
CPC分类号： C12P7/04
摘要： A method of producing isopropyl alcohol includes: culturing an isopropyl alcohol-producing Escherichia coli under a bacterial cell growth condition in which the Escherichia coli stably proliferates in an isopropyl alcohol production period while continuously supplying a substrate solution to a culture tank and continuously removing a product-containing culture solution from the culture tank, the substrate solution containing a plant-derived raw material, the number of cells of the isopropyl alcohol-producing Escherichia coli in the culture tank being maintained during the culturing, and the isopropyl alcohol-producing Escherichia coli having isopropyl alcohol production ability introduced or modified by genetic recombination; bringing the isopropyl-alcohol-producing Escherichia coli into contact with the plant-derived raw material in the culture tank to produce isopropyl alcohol; and recovering the isopropyl alcohol produced by the isopropyl alcohol-producing Escherichia coli from the culture solution that contains the product and that has been removed from the culture tank.
摘要翻译：异丙醇的制造方法包括：在异丙醇生产期间，在大肠杆菌稳定地增殖的细菌细胞生长条件下培养产生异丙醇的大肠杆菌，同时将培养液连续地供给到培养槽中并连续地除去产物含有植物来源的原料的底物溶液，培养槽内维持的异丙醇生产性大肠杆菌的细胞数和产生异丙醇的大肠杆菌通过基因重组引入或修饰异丙醇生产能力; 将产生异丙醇的大肠杆菌与培养罐中的植物来源的原料接触以产生异丙醇; 并从含有产物的培养液中回收由产生异丙醇的大肠杆菌产生的异丙醇，并从培养槽中取出。

7. 发明申请

US20110288255A1 METHOD FOR PRODUCING AMIDE COMPOUND 有权
标题翻译：生产酰胺化合物的方法
公开(公告)号：US20110288255A1
公开(公告)日：2011-11-24
申请号：US13197066
申请日：2011-08-03
申请人： Hiroko Shibamoto , Toshikazu Aikawa , Teruo Arii , Masanori Muramoto , Takeshi Fukuda , Kiyoshi Ito , Takeya Abe , Souichi Hazama
发明人： Hiroko Shibamoto , Toshikazu Aikawa , Teruo Arii , Masanori Muramoto , Takeshi Fukuda , Kiyoshi Ito , Takeya Abe , Souichi Hazama
IPC分类号： C08F20/56 , C12P13/02
CPC分类号： C12P13/00 , C07C231/06 , C08F220/56 , C12P13/02 , C07C233/09
摘要： A method for producing an amide compound from a nitrile compound in an aqueous medium in the presence of a catalyst having a nitrile hydratase activity wherein the concentration of benzene in the aqueous medium is 4.0 ppm or less and a method for producing an amide-based polymer excellent in quality from the amide compound. Also provided are a method for more efficiently producing an acrylamide with higher quality by a microbial catalyst containing a nitrile hydratase and the like and a method for producing an acrylamide-based polymer, which is excellent in hue, has a good balance between water solubility and high molecular weight and is excellent in quality.
摘要翻译：在具有腈水合酶活性的催化剂存在下，在含水介质中由腈化合物制备酰胺化合物的方法，其中水介质中苯的浓度为4.0ppm或更低，以及制备酰胺基聚合物的方法优异的酰胺化合物。还提供了通过含有腈水合酶等的微生物催化剂更有效地制备具有更高质量的丙烯酰胺的方法和色调优异的丙烯酰胺类聚合物的制造方法，水溶性与高分子量，质量优良。

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