专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08236532B2 Multibase delivery for long reads in sequencing by synthesis protocols 有权
标题翻译：通过合成方案进行长序读取的多碱基递送
公开(公告)号：US08236532B2
公开(公告)日：2012-08-07
申请号：US12641104
申请日：2009-12-17
申请人： Mostafa Ronaghi , Helmy A. Eltoukhy
发明人： Mostafa Ronaghi , Helmy A. Eltoukhy
IPC分类号： C12P19/34
CPC分类号： C12Q1/6874 , C12Q1/6837 , C12Q1/6869 , C12Q2535/122 , C12Q2565/543
摘要： Methods for obtaining nucleic acid sequence information including the steps of (a) providing a first sequencing reagent to a target nucleic acid in the presence of a polymerase, wherein the first sequencing reagent includes at least two different nucleotide monomers and no more than three nucleotide monomers, wherein the nucleotide monomers are simultaneously in the presence of the target nucleic acid, (b) providing a second sequencing reagent to the target nucleic acid, wherein the second sequencing reagent comprises one or more nucleotide monomers, at least one of the one or more nucleotide monomers being different from the nucleotide monomers present in the first sequencing reagent, and wherein the second sequencing reagent is provided subsequent to providing the first sequencing reagent, and optionally (c) repeating (a) and (b) for said target nucleic acid, whereby sequence information for at least a portion of the target nucleic acid is obtained.
摘要翻译：用于获得核酸序列信息的方法，包括以下步骤：（a）在聚合酶存在下向靶核酸提供第一测序试剂，其中所述第一测序试剂包含至少两种不同的核苷酸单体和不超过三个核苷酸单体其中所述核苷酸单体同时存在于靶核酸的存在下，（b）向靶核酸提供第二测序试剂，其中所述第二测序试剂包含一种或多种核苷酸单体，所述一种或多种核苷酸单体不同于存在于第一测序试剂中的核苷酸单体，并且其中在提供第一测序试剂后提供第二测序试剂，以及任选（c）对所述靶核酸重复（a）和（b）由此获得靶核酸的至少一部分的序列信息。

2. 发明申请

US20090286299A1 ENGINEERED LUCIFERASES 审中-公开
标题翻译：工程机械
公开(公告)号：US20090286299A1
公开(公告)日：2009-11-19
申请号：US12467222
申请日：2009-05-15
申请人： Mostafa Ronaghi , Helmy A. Eltoukhy , Leila Bazargan
发明人： Mostafa Ronaghi , Helmy A. Eltoukhy , Leila Bazargan
IPC分类号： C12N9/02 , C12N11/00
CPC分类号： C12Q1/66 , C12N9/0069
摘要： DNA sequencing techniques are important for a variety of research and diagnostic applications. Pyrosequencing is a “sequencing by synthesis” technique that makes use of luciferase. Modified luciferase enzymes and methods of DNA pyrosequencing are provided. Means of preparing and producing mutant luciferases that have enhanced selectivity for ATP or dATP are described.
摘要翻译： DNA测序技术对于各种研究和诊断应用很重要。焦磷酸测序是使用荧光素酶的“通过合成测序”技术。提供了改良的荧光素酶和DNA焦磷酸测序方法。描述了制备和产生具有增强的ATP或dATP选择性的突变荧光素酶的方法。

3. 发明申请

US20120295262A1 MULTIBASE DELIVERY FOR LONG READS IN SEQUENCING BY SYNTHESIS PROTOCOLS 有权
标题翻译：通过合成方案进行长时间读取的多条交付
公开(公告)号：US20120295262A1
公开(公告)日：2012-11-22
申请号：US13545682
申请日：2012-07-10
申请人： Mostafa Ronaghi , Helmy A. Eltoukhy
发明人： Mostafa Ronaghi , Helmy A. Eltoukhy
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6837 , C12Q1/6869 , C12Q2535/122 , C12Q2565/543
摘要： The present technology relates to molecular sciences, such as genomics. More particularly, the present technology relates to methods for obtaining long lengths of sequencing data.
摘要翻译：本技术涉及分子科学，如基因组学。更具体地，本技术涉及用于获得长长度的测序数据的方法。

4. 发明授权

US09096899B2 Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same 有权
标题翻译：用于生物或化学分析的微型设备和生物传感器盒及其系统和方法
公开(公告)号：US09096899B2
公开(公告)日：2015-08-04
申请号：US13882088
申请日：2011-10-20
申请人： Helmy A. Eltoukhy , Tarun Khurana , Behnam Javanmardi , Poorya Sabounchi , Majid Aghababazadeh
发明人： Helmy A. Eltoukhy , Tarun Khurana , Behnam Javanmardi , Poorya Sabounchi , Majid Aghababazadeh
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48 , C12Q1/68 , B01L3/00 , B01L9/00 , G01N21/05 , G01N21/64 , B01L7/00 , G01N21/11 , G01N35/10 , G01N21/03
CPC分类号： C12Q1/6874 , B01L3/502715 , B01L3/502746 , B01L3/5085 , B01L7/52 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0877 , G01N21/05 , G01N21/11 , G01N21/6428 , G01N21/6452 , G01N35/1097 , G01N2021/0346 , G01N2021/058
摘要： Biosensor cartridge includes a flow cell having inlet and outlet ports and a flow channel that extends therebetween. The flow cell includes a substrate field having a plurality of reaction chambers. The reaction chambers have apertures that open onto the flow channel. The biosensor cartridge also includes an activity detector that is coupled to the flow cell and has an array of pixels that has a fixed position relative to the substrate field of the flow cell. The pixels are assigned to select reaction chambers such that activity detected by the pixels indicates that a desired reaction has occurred within the select reaction chamber. The biosensor cartridge also includes an exterior side surface having a plurality of electrical contacts thereon that are communicatively coupled to the pixels. The electrical contacts of the side surface are configured to engage corresponding mating contacts of a bioassay system.
摘要翻译：生物传感器盒包括具有入口和出口的流动池和在其间延伸的流动通道。流动池包括具有多个反应室的基底场。反应室具有通向流动通道的孔。生物传感器盒还包括活动检测器，该活动检测器耦合到流动池并且具有相对于流动池的衬底场具有固定位置的像素阵列。分配像素以选择反应室，使得由像素检测的活性表示在选择反应室内已经发生期望的反应。生物传感器盒还包括外部侧表面，其上具有多个电连接到像素上的电触点。侧表面的电触点被构造成接合生物测定系统的相应配合触点。

5. 发明授权

US08476022B2 Method of making an array of nucleic acid colonies 有权
标题翻译：制作核酸集落阵列的方法
公开(公告)号：US08476022B2
公开(公告)日：2013-07-02
申请号：US13545682
申请日：2012-07-10
申请人： Mostafa Ronaghi , Helmy A. Eltoukhy
发明人： Mostafa Ronaghi , Helmy A. Eltoukhy
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6874 , C12Q1/6837 , C12Q1/6869 , C12Q2535/122 , C12Q2565/543
摘要： A method of making an array of nucleic acid colonies, by (a) providing a substrate having a patterned surface of features, wherein the features are spatially separated from each other on the surface of the substrate; (b) contacting the substrate with a solution of different target nucleic acids to seed a subset of the features that contact the solution, wherein each feature in the subset is seeded with a single nucleic acid from the solution, wherein a plurality of the features that contact the solution are not seeded with a nucleic acid from the solution; (c) amplifying the nucleic acids to form a nucleic acid colony at each of the features in the subset; and (d) repeating steps (b) and (c) to increase the number of the features on the surface that have a nucleic acid colony, thereby making an array of nucleic acid colonies.
摘要翻译：通过（a）提供具有特征图案化表面的基底，制备核酸集落阵列的方法，其中所述特征在所述基底的表面上彼此空间上分离; （b）使所述底物与不同靶核酸的溶液接触以接种与所述溶液接触的特征的子集，其中所述亚组中的每个特征从所述溶液中接种单个核酸，其中所述多个特征接触溶液不会从溶液中接种核酸; （c）扩增核酸以在子集中的每个特征上形成核酸集落; 和（d）重复步骤（b）和（c）以增加具有核酸集落的表面上的特征的数目，由此形成核酸集落阵列。

6. 发明申请

US20130040863A1 SOLID-PHASE CLONAL AMPLIFICATION AND RELATED METHODS 有权
公开(公告)号：US20130040863A1
公开(公告)日：2013-02-14
申请号：US13637770
申请日：2011-03-16
申请人： Neil A. Straus , Shengrong Lin , Helmy A. Eltoukhy , Kevin Gunderson
发明人： Neil A. Straus , Shengrong Lin , Helmy A. Eltoukhy , Kevin Gunderson
IPC分类号： C40B50/06 , C40B50/18
CPC分类号： C12Q1/6874 , C12Q1/6834 , C12Q1/6837 , C12Q2531/125 , C12Q2563/149
摘要： The present invention provides methods and compositions for analyzing nucleic acid sequences. In some aspects, the methods utilize clonal objects, such as DNA balls, that have been captured on beads. Using the methods described here, compositions are fabricated wherein a bead and one clonal object are affinity bound or hybridized to each other through an affinity binding patch or hybridization patch on the surface of the bead. The invention also provides a population of beads having affinity bound or hybridized clonal objects at a ratio of 1:1. The invention additionally provides methods for amplifying a target nucleic acid molecule utilizing the compositions described herein.

7. 发明授权

US08951940B2 Solid-phase clonal amplification and related methods 有权
标题翻译：固相克隆扩增及相关方法
公开(公告)号：US08951940B2
公开(公告)日：2015-02-10
申请号：US13637770
申请日：2011-03-16
申请人： Neil A. Straus , Shengrong Lin , Helmy A. Eltoukhy , Kevin Gunderson
发明人： Neil A. Straus , Shengrong Lin , Helmy A. Eltoukhy , Kevin Gunderson
IPC分类号： C40B50/06 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6874 , C12Q1/6834 , C12Q1/6837 , C12Q2531/125 , C12Q2563/149
摘要： The present invention provides methods and compositions for analyzing nucleic acid sequences. In some aspects, the methods utilize clonal objects, such as DNA balls, that have been captured on beads. Using the methods described here, compositions are fabricated wherein a bead and one clonal object are affinity bound or hybridized to each other through an affinity binding patch or hybridization patch on the surface of the bead. The invention also provides a population of beads having affinity bound or hybridized clonal objects at a ratio of 1:1. The invention additionally provides methods for amplifying a target nucleic acid molecule utilizing the compositions described herein.
摘要翻译：本发明提供了用于分析核酸序列的方法和组合物。在某些方面，该方法利用已被捕获在珠上的克隆物体，例如DNA球。使用本文描述的方法，制备组合物，其中珠粒和一个克隆物体通过珠粒表面上的亲和结合片段或杂交片段彼此亲和地结合或杂交。本发明还提供了以1:1的比例具有亲和结合或杂交的克隆物体的珠群。本发明另外提供了利用本文所述组合物扩增靶核酸分子的方法。

8. 发明申请

US20130210682A1 MICRODEVICES AND BIOSENSOR CARTRIDGES FOR BIOLOGICAL OR CHEMICAL ANALYSIS AND SYSTEMS AND METHODS FOR THE SAME 有权
公开(公告)号：US20130210682A1
公开(公告)日：2013-08-15
申请号：US13882088
申请日：2011-10-20
申请人： Helmy A. Eltoukhy , Tarun Khurana , Behnam Javanmardi , Poorya Sabounchi , Majid Aghababazadeh
发明人： Helmy A. Eltoukhy , Tarun Khurana , Behnam Javanmardi , Poorya Sabounchi , Majid Aghababazadeh
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , B01L3/502715 , B01L3/502746 , B01L3/5085 , B01L7/52 , B01L9/527 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0877 , G01N21/05 , G01N21/11 , G01N21/6428 , G01N21/6452 , G01N35/1097 , G01N2021/0346 , G01N2021/058
摘要： A flow cell including inlet and outlet ports in fluid communication with each other through a flow channel that extends therebetween. The flow channel includes a diffuser region and a field region that is located downstream from the diffuser region. The field region of the flow channel directs fluid along reaction sites where desired reactions occur. The fluid flows through the diffuser region in a first flow direction and through the field region in a second flow direction. The first and second flow directions being substantially perpendicular.

9. 发明申请

US20100173303A1 MULTIBASE DELIVERY FOR LONG READS IN SEQUENCING BY SYNTHESIS PROTOCOLS 有权
标题翻译：通过合成方案进行长时间读取的多条交付
公开(公告)号：US20100173303A1
公开(公告)日：2010-07-08
申请号：US12641104
申请日：2009-12-17
申请人： Mostafa Ronaghi , Helmy A. Eltoukhy
发明人： Mostafa Ronaghi , Helmy A. Eltoukhy
IPC分类号： C12Q1/68 , G06K9/62
CPC分类号： C12Q1/6874 , C12Q1/6837 , C12Q1/6869 , C12Q2535/122 , C12Q2565/543
摘要： The present technology relates to molecular sciences, such as genomics. More particularly, the present technology relates to methods for obtaining long lengths of sequencing data.
摘要翻译：本技术涉及分子科学，如基因组学。更具体地，本技术涉及用于获得长长度的测序数据的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式