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1. 发明授权

US4472498A Analysis film and a method of analysis using the same 失效
标题翻译：分析胶片和使用其的分析方法
公开(公告)号：US4472498A
公开(公告)日：1984-09-18
申请号：US401771
申请日：1982-07-26
申请人： Nobuhito Masuda , Yukio Yasuda , Shigeru Nagatomo , Hajime Makiuchi , Masaki Okazaki
发明人： Nobuhito Masuda , Yukio Yasuda , Shigeru Nagatomo , Hajime Makiuchi , Masaki Okazaki
IPC分类号： G01N33/52 , G01N33/535 , G01N33/542 , G01N33/543 , G01N33/54 , G01N33/78
CPC分类号： G01N33/542 , G01N33/521 , G01N33/535 , G01N33/54386 , Y10S435/805 , Y10S435/97 , Y10S436/81 , Y10S436/815
摘要： An analysis film comprises a reagent layer composed of a porous material which contains an antibody but does not substantially contain a complex of an analyte or a labelled antigen with the antibody. In the analysis film, reagents for enzyme immune reaction of homogenous type are incorporated so that an analyte is analyzed without requiring B/F separation. An analysis method for various analytes using the same provides high sensitivity, high accuracy as well as good reproducibility and is simple and rapid.
摘要翻译：分析膜包括由含有抗体但基本上不含有分析物或标记抗原与抗体的复合物的多孔材料构成的试剂层。在分析膜中，并入用于均匀型酶免疫反应的试剂，从而分析分析物而不需要B / F分离。使用该方法的各种分析物的分析方法提供高灵敏度，高精度以及良好的再现性，并且简单快速。

2. 发明授权

US4884213A Method for quantitative analysis of analyte in liquid sample using analytical element 失效
标题翻译：使用分析元素定量分析液体样品中分析物的方法
公开(公告)号：US4884213A
公开(公告)日：1989-11-28
申请号：US311444
申请日：1989-02-15
申请人： Yuzo Iwata , Hajime Makiuchi , Masao Kitajima
发明人： Yuzo Iwata , Hajime Makiuchi , Masao Kitajima
IPC分类号： G01N21/47 , C12Q1/54 , C12Q1/58 , G01N21/27 , G01N21/86 , G01N33/52
CPC分类号： C12Q1/58 , C12Q1/54 , G01N21/274 , G01N33/525 , G01N21/4738 , G01N21/8483
摘要： In a method for quantitatively analyzing an analyte in a liquid sample by applying the liquid sample on one of a set of analytical elements and measuring a color developed in the analytical element through reflection photometory in which said set of analytical elements are the same elements as a standard element except for deviation of sensitivity to the analyte, but said deviation of sensitivity is essentially equivalent to each other among the set of analytical elements, the calibration curve of the standard element with respect to said analyte being predetermined, an improvement involving the preparation of a linear equation for defining a relationship between an optical reflection density value OD.sub.x to be measured on an analytical element belonging to said set of the analytical elements and an optical reflection density value OD.sub.s being predetermined on the standard analytical element, in which the optical reflection density values OD.sub.x and OD.sub.s are those to be determined on colors developed on the respective elements upon application of a liquid sample containing the analyte in the same content C.sub.x.
摘要翻译：在通过将液体样品应用于一组分析元素之一并通过反射光度测量在分析元件中显影的颜色来定量分析液体样品中的分析物的方法中，其中所述一组分析元件是与除了对分析物的敏感性偏差之外，所述灵敏度的偏差在所述一组分析元件中基本相同，所述标准元件相对于所述分析物的校准曲线是预定的，改进涉及制备用于定义属于所述分析单元组的分析单元的光反射浓度值ODx与标准分析单元上规定的光反射浓度值ODs之间的关系的线性方程式，其中光学反射密度值ODx和OD是在颜色dev上确定的值在将含有相同含量C x的分析物的液体样品应用于各个元件上。

3. 发明授权

US5348889A Method for correcting a calibration curve 失效
标题翻译：修正校准曲线的方法
公开(公告)号：US5348889A
公开(公告)日：1994-09-20
申请号：US940770
申请日：1992-09-04
申请人： Masaaki Terashima , Hajime Makiuchi
发明人： Masaaki Terashima , Hajime Makiuchi
IPC分类号： G01N21/75 , G01N21/27 , G01N21/77 , G01N21/86 , G01N31/00 , G01N25/00
CPC分类号： G01N21/274 , G01N21/8483 , Y10T436/10
摘要： An improved calibration method for calibrating the measured value of an analyte in a liquid sample by referring to a calibration curve drawn by plotting the interrelation between the calibration values of n (n is an integer of not less than 2) calibrators containing different contents of the analyte and the measured values of the analyte contained in respective calibrators. In the improved method provided by the invention, the function of the calibration curve is determined by a first step of estimating an imaginal point, the imaginal point either corresponding to a calibration value larger than the uppermost value of the n calibrators and vicinal to the upper limit of the determination range of the analyte or corresponding to a calibration value smaller than the lowermost value of the n calibrators and vicinal to the lower limit of the determination range of the analyte or a calibration value vicinal to the zero value, and a second step of determining the function of the calibration curve by plotting the imaginal point with two to n measured values.
摘要翻译：一种改进的校准方法，用于通过参考通过绘制n（n是不小于2的整数）的校准器之间的相互关系绘制的校准曲线来校准液体样品中的分析物的测量值，所述校准值包含不同内容分析物和各自校准物中所含分析物的测量值。在本发明提供的改进方法中，通过估计成像点的第一步骤来确定校准曲线的功能，该成像点对应于校准值大于n个校准器的最上面的值，并且与上位点相邻分析物的确定范围的极限或对应于小于n个校准品的最低值的校准值，并且与分析物的确定范围的下限相邻，或与零值邻近的校准值，以及第二步骤通过用2到n个测量值绘制想象点来确定校准曲线的功能。

4. 发明授权

US5047351A Optical end-point type analytical method 失效
标题翻译：光端点型分析方法
公开(公告)号：US5047351A
公开(公告)日：1991-09-10
申请号：US351315
申请日：1989-05-10
申请人： Hajime Makiuchi , Yuzo Iwata , Kikuo Hirai , Kenji Murabayashi
发明人： Hajime Makiuchi , Yuzo Iwata , Kikuo Hirai , Kenji Murabayashi
IPC分类号： C12Q1/00 , G01N21/27 , G01N21/75 , G01N21/78 , G01N31/22 , G01N33/52
CPC分类号： C12Q1/00 , G01N21/272 , G01N33/521 , G01N2021/174 , G01N21/78
摘要： A method for shortening the analysis time, without adversely affecting the accuracy in the colorimetric analysis of an analyte. The method including the steps of:(a) Measuring the optical density for two or more times at suitable time intervals after the beginning of reaction to follow the variation of optical density,(b) Judging based upon the optical density obtained at the earlier measurement whether the reaction is to be further continued or not,(c) When it is judged that the continuation of the reaction is not necessary in terms of analytical accuracy, selecting the calibration curve corresponding to the reaction time,(d) While, when it is judged that the continuation of the reaction is necessary, further continuing the reaction, and(e) Repeating steps (b) and (d) until the judgement that the continuation of the reaction is not necessary is obtained.
摘要翻译：用于缩短分析时间的方法，而不会不利地影响分析物的比色分析的准确性。该方法包括以下步骤：（a）在反应开始后以合适的时间间隔测量光密度两次或更多次，以遵循光密度的变化，（b）基于在较早测量时获得的光密度反应是否进一步持续，（c）当判断在分析精度方面不需要继续反应时，选择与反应时间对应的校准曲线，（d）当判断反应的持续是必要的，进一步继续反应，（e）重复步骤（b）和（d），直到判断出不需要反应的延续为止。

5. 发明授权

US4861552A Biological reaction layer and its preparation 失效
标题翻译：生物反应层及其制备
公开(公告)号：US4861552A
公开(公告)日：1989-08-29
申请号：US851878
申请日：1986-04-11
申请人： Nobuhito Masuda , Shigeru Nagatomo , Hajime Makiuchi , Yukio Yasuda
发明人： Nobuhito Masuda , Shigeru Nagatomo , Hajime Makiuchi , Yukio Yasuda
IPC分类号： G01N33/52 , C12M1/00 , G01N33/543 , G01N33/78 , C12Q1/00 , G01N33/548
CPC分类号： G01N33/78 , G01N33/54386 , Y10S435/805 , Y10S436/81
摘要： A biological reaction layer of a dry analytical element in the form of a composite porous layer in which a particulate material carrying a biologically active material fixed thereon is dispersed in a fibrous porous matrix, characterized in that the dry weight ratio of said particulate material to said fibrous material contained in said composite porous layer ranges from 1:20 to 1:0.3 and said particulate material is contained therein in the range of 1 to 60 g/m.sup.2.
摘要翻译：复合多孔层形式的干燥分析元件的生物反应层，其中载有固定在其上的生物活性材料的颗粒材料分散在纤维多孔基质中，其特征在于，所述颗粒材料与所述所述复合多孔层中含有的纤维状物质的含量为1:20〜1:0.3，所述粒状物质的含量为1〜60g / m 2。

6. 发明授权

US4414325A Method for measurement of trace enzyme 失效
标题翻译：痕量酶的测定方法
公开(公告)号：US4414325A
公开(公告)日：1983-11-08
申请号：US298814
申请日：1981-09-02
申请人： Nobuhito Masuda , Yuji Mihara , Masaki Okazaki , Hajime Makiuchi
发明人： Nobuhito Masuda , Yuji Mihara , Masaki Okazaki , Hajime Makiuchi
IPC分类号： G01N33/536 , C12Q1/00 , C12Q1/34 , G01N33/53 , G01N33/52 , G01N33/54 , G01N33/58
CPC分类号： C12Q1/34 , C12Q1/00 , C12Q2334/00 , C12Q2337/00 , G01N2333/976 , Y10S435/962 , Y10S436/805
摘要： The amount of enzyme is determined by reacting an enzyme to be measured with a synthetic substrate having at least one structure to be specifically contacted with the enzyme and at least one spectral sensitizer structure, bringing either the reaction product having the spectral sensitizer structure or the unreacted synthetic substrate into contact with a silver halide emulsion layer, exposing the same to light of a spectral sensitizing wavelength region corresponding to the spectral sensitizer structure, developing the silver halide, and then measuring the optical density of developed silver and/or colored dye.
摘要翻译：酶的量通过使待测量的酶与具有至少一种与酶特异性接触的结构的合成底物和至少一种光谱增感剂结构反应，使具有光谱增感剂结构的反应产物或未反应的合成基底与卤化银乳剂层接触，将其暴露于对应于光谱敏化剂结构的光谱增感波长区域的光，显影卤化银，然后测量显影的银和/或着色染料的光密度。

7. 发明授权

US4587102A Multi-layer analysis element utilizing specific binding reaction 失效
标题翻译：利用特异性结合反应的多层分析元件
公开(公告)号：US4587102A
公开(公告)日：1986-05-06
申请号：US637324
申请日：1984-08-02
申请人： Shigeru Nagatomo , Yukio Yasuda , Nobuhito Masuda , Hajime Makiuchi , Masaki Okazaki
发明人： Shigeru Nagatomo , Yukio Yasuda , Nobuhito Masuda , Hajime Makiuchi , Masaki Okazaki
IPC分类号： G01N33/58 , G01N33/52 , G01N33/543 , G01N21/78 , G01N33/53
CPC分类号： G01N33/54386 , G01N33/525 , Y10S435/805 , Y10S436/81
摘要： A dry type multilayer analysis element for assaying a concentration of a specific component utilizing a competitive immunological reaction comprises a detection element comprising a detection layer which receives a labelled complex formed as a result of the competitive immunological reaction or an optically detectable change formed dependent upon an amount of the labelled complex of the specific component and having further provided thereon the detection layer a reaction layer comprising a fibrous porous medium containing fine particles therein. The multilayer analysis element absorbs an amount of a sample solution necessary for the competitive immunological reaction so that the multilayer analysis element has high sensitivity and high reproducibility.
摘要翻译：用于使用竞争性免疫反应测定特定成分的浓度的干式多层分析元件包括检测元件，其包含检测层，该检测层接受由于竞争性免疫反应而形成的标记复合物，或者由量的特定成分的标记复合物，并且还在其上提供检测层，反应层包含其中含有微粒的纤维状多孔介质。多层分析元件吸收竞争性免疫反应所需的样品溶液的量，使得多层分析元件具有高灵敏度和高再现性。

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