专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06210928B1 Production of proteins in procaryotes 失效
标题翻译：在原核生产蛋白质
公开(公告)号：US06210928B1
公开(公告)日：2001-04-03
申请号：US09066288
申请日：1998-04-24
申请人： Gwen G. Krivi
发明人： Gwen G. Krivi
IPC分类号： C12P2102
CPC分类号： A23K20/184 , A23K20/168 , C07K14/61
摘要： A method for preparing polypeptides in bacteria with an alanine rather than a methionine at the N-terminus. The DNA sequence expressed has an alanine codon immediately following from one to about three contiguous methionine codons including a translation start signal and allows for the expression of polypeptides having the amino acid sequence of, for example, naturally occurring eucaryotic proteins such as various bovine and porcine somatotropin species.
摘要翻译：在N-末端用丙氨酸而不是甲硫氨酸在细菌中制备多肽的方法。所表达的DNA序列具有紧邻从一至约三个连续甲硫氨酸密码子（包括翻译起始信号）的丙氨酸密码子，并允许表达具有例如天然存在的真核生物蛋白如多种牛和猪的氨基酸序列的多肽生长激素种类。

2. 发明授权

US5374523A Allelic variants of bovine somatotropin gene:genetic marker for superior milk production in bovine 失效
标题翻译：牛生长激素基因的等位变体：牛中优质牛奶生产的遗传标记
公开(公告)号：US5374523A
公开(公告)日：1994-12-20
申请号：US926785
申请日：1992-08-10
申请人： Robert J. Collier , Scott D. Hauser , Gwen G. Krivi , Matthew C. Lucy
发明人： Robert J. Collier , Scott D. Hauser , Gwen G. Krivi , Matthew C. Lucy
IPC分类号： C12Q1/68 , C07H17/00 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q2600/124 , C12Q2600/156
摘要： An assay for determining the presence in bovine genetic material of a genetic marker located on the bovine somatotropin gene indicative of an inheritable trait of increased milk production is provided. The marker is a polymorphism in the gene encoding somatotropin at amino acid position 126 which results in two forms of somatotropin existing in bovine. The assay comprises analyzing a bovine to determine its genotype with respect to the somatotropin gene. Bovine with the desired marker can be selected for inclusion in breeding programs or for milking. The desired marker indicative of superior milk production is dependent upon the breed of cattle. Holstein cattle that are homozygous for the leucine forms of somatotropin are desired. Jersey cattle that are homozygous for the valine form of somatotropin are desired. A kit for performing the assay is also provided.
摘要翻译：提供了一种用于确定位于牛生长激素基因上的遗传标记的牛遗传物质中存在的测定法，其指示增加的产奶量的可遗传性状。该标记是在氨基酸位置126编码生长激素的基因中的多态性，其导致存在于牛中的两种形式的生长激素。该测定法包括分析牛，以确定其相对于生长激素基因的基因型。可以选择具有所需标记的牛来包括在育种程序中或用于挤奶。指示优质牛奶生产的期望标记取决于牛的品种。希望对于生精肌氨酸的亮氨酸形式是纯合的荷斯坦牛。需要针对缬氨酸形式的生长激素纯合的泽西牛。还提供了用于进行测定的试剂盒。

3. 发明授权

US5037806A Biologically active method using somatotropins 失效
标题翻译：使用生长激素的生物活性方法
公开(公告)号：US5037806A
公开(公告)日：1991-08-06
申请号：US824202
申请日：1986-02-04
申请人： Gwen G. Krivi
发明人： Gwen G. Krivi
IPC分类号： C12N15/09 , A23K1/165 , A23K1/18 , A61K35/74 , A61K38/27 , A61P43/00 , C07H21/04 , C07K14/00 , C07K14/195 , C07K14/41 , C07K14/575 , C07K14/61 , C12N20060101 , C12N1/21 , C12N15/00 , C12N15/18 , C12N15/74 , C12P21/00 , C12P21/02 , C12R1/19
CPC分类号： C07K14/61 , A23K20/168
摘要： A method for preparing polypeptides in bacteria with an alanine rather than a methionine at the N-terminus. The DNA sequence expressed has an alanine codon immediately following from at least one methionine codon, and allows for the expression of polypeptides having the amino acid sequence essentially the same as such naturally occurring eucaryotic protein as various bovine and porcine somatotropin species. In another important embodiment, the invention provides a class of compositions comprising certain valine-containing somatropin species which increase milk production in lactating mammals to a significantly greater extent than otherwise-identical leucine somatotropin species.
摘要翻译：在N-末端用丙氨酸而不是甲硫氨酸在细菌中制备多肽的方法。表达的DNA序列具有紧邻从至少一个甲硫氨酸密码子之后的丙氨酸密码子，并且允许表达具有与诸如牛和猪生长激素类物种的天然存在的真核蛋白基本上相同的氨基酸序列的多肽。在另一个重要的实施方案中，本发明提供了一类包含某些含缬氨酸的生长激素类物质的组合物，其在哺乳哺乳动物中的乳产量比其他相同的亮氨酸生长激素物质显着地增加。

4. 发明授权

US4783524A Growth factors 失效
标题翻译：生长因子
公开(公告)号：US4783524A
公开(公告)日：1988-11-08
申请号：US888996
申请日：1986-07-31
申请人： Brent R. Larsen , Ned R. Siegel , Claire E. Kotts , Michael F. McGrath , Sharon D. Ogden , Gwen G. Krivi , John C. Minnerly
发明人： Brent R. Larsen , Ned R. Siegel , Claire E. Kotts , Michael F. McGrath , Sharon D. Ogden , Gwen G. Krivi , John C. Minnerly
IPC分类号： C12N15/09 , A23K1/16 , A61K20060101 , A61K35/74 , A61K38/00 , A61K38/27 , A61K38/28 , A61P5/00 , C07H21/04 , C07K14/00 , C07K14/65 , C07K16/00 , C12N20060101 , C12N15/00 , C12P20060101 , C12P21/02 , C07K13/00
CPC分类号： C07K14/65 , A23K20/147 , A61K38/00 , Y10S930/12
摘要： This invention relates to novel peptides having utility for promotion of growth and/or lactation in animals, to processes and DNA useful in production of such peptides, and to methods utilizing such peptides to promote growth or lactation in animals. In some embodiments, the invention is directed to peptides having bovine IGF-II biological activity, to production of such peptides, and to their use in effecting proliferation of certain cells (e.g. mammary epithelial or muscle) or in enhancing lactation in cattle or other animals.
摘要翻译：本发明涉及可用于促进动物生长和/或哺乳的新型肽，可用于生产这些肽的方法和DNA，以及利用这些肽促进动物生长或哺乳的方法。在一些实施方案中，本发明涉及具有牛IGF-II生物活性的肽，生产这样的肽，以及用于实现某些细胞（例如乳腺上皮或肌肉）的增殖或增强牛或其他动物的哺乳期。

5. 发明授权

US5744328A Production of proteins in procaryotes 失效
公开(公告)号：US5744328A
公开(公告)日：1998-04-28
申请号：US402088
申请日：1995-03-10
申请人： Gwen G. Krivi
发明人： Gwen G. Krivi
IPC分类号： C12N15/00 , A23K1/165 , C07K14/61 , C12N15/18
CPC分类号： C07K14/61 , A23K20/168
摘要： A method for preparing polypeptides in bacteria with an alanine rather than a methionine at the N-terminus. The DNA sequence expressed has an alanine codon immediately following from one to about three contiguous methionine codons including a translation start signal and allows for the expression of polypeptides having the amino acid sequence of, for example, naturally occurring eucaryotic proteins such as various bovine and porcine somatotropin species.

6. 发明授权

US5141922A Biologically active proteins and a method for their use 失效
标题翻译：生物活性蛋白及其使用方法
公开(公告)号：US5141922A
公开(公告)日：1992-08-25
申请号：US729283
申请日：1991-07-12
申请人： Gwen G. Krivi
发明人： Gwen G. Krivi
IPC分类号： A23K1/165 , C07K14/61
CPC分类号： C07K14/61 , A23K20/168
摘要： A method for preparing polypeptides in bacteria with an alanine rather than a methionine at the N-terminus. The DNA sequence expressed has an alanine codon immediately following from at least one methionine codon, and allows for the expression of polypeptides having the amino acid sequence essentially the same as such naturally occurring eucaryotic protein as various bovine and porcine somatotropin species. In another important embodiment, the invention provides a class of compositions comprising certain valine-containing somatropin species which increase milk production in lactating mammals to a significantly greater extent than otherwise-identical leucine somatotropin species.
摘要翻译：在N-末端用丙氨酸而不是甲硫氨酸在细菌中制备多肽的方法。表达的DNA序列具有紧邻从至少一个甲硫氨酸密码子之后的丙氨酸密码子，并且允许表达具有与诸如牛和猪生长激素类物种的天然存在的真核蛋白基本上相同的氨基酸序列的多肽。在另一个重要的实施方案中，本发明提供了一类包含某些含缬氨酸的生长激素类物质的组合物，其在哺乳哺乳动物中的乳产量比其他相同的亮氨酸生长激素物质显着地增加。

7. 发明授权

US5115102A Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices 失效
标题翻译：改善金属物质基质的增强性能的变种蛋白质和多肽
公开(公告)号：US5115102A
公开(公告)日：1992-05-19
申请号：US383778
申请日：1989-07-21
申请人： Barry L. Haymore , Gary S. Bild , Gwen G. Krivi
发明人： Barry L. Haymore , Gary S. Bild , Gwen G. Krivi
IPC分类号： A23K1/18 , A23K1/16 , B01J20/281 , C07K1/107 , C07K1/14 , C07K1/16 , C07K1/22 , C07K14/00 , C07K14/575 , C07K14/61 , C07K14/65 , C12N15/16 , C12N15/18 , G01N30/88
CPC分类号： C07K14/61 , C07K1/107 , C07K14/65
摘要： The present invention is directed at variant proteins and polypeptides having an enhanced affinity, i.e., greater binding strength, for immobilized-metal affinity resins and resides in engineering one or more specific metal-chelating amino acid sequences into a protein or polypeptide, the specific sequence depending on the metal-binding amino acids utilized and the secondary structure associated with the portion of the protein or polypeptide to include such sequence.

8. 发明授权

US4981956A DNA sequences encoding bovine growth factors 失效
标题翻译：编码牛生长因子的DNA序列
公开(公告)号：US4981956A
公开(公告)日：1991-01-01
申请号：US254332
申请日：1988-10-06
申请人： Brent R. Larsen , Ned R. Siegel , Claire E. Kotts , Michael F. McGrath , Sharon D. Ogden , Gwen G. Krivi , John C. Minnerly
发明人： Brent R. Larsen , Ned R. Siegel , Claire E. Kotts , Michael F. McGrath , Sharon D. Ogden , Gwen G. Krivi , John C. Minnerly
IPC分类号： A23K1/16 , A61K38/00 , C07K14/65
CPC分类号： C07K14/65 , A23K20/147 , A61K38/00
摘要： This invention relates to novel peptides having utility for promotion of growth and/or lactation in animals, to processes and DNA useful in production of such peptides, and to methods utilizing such peptides to promote growth or lactation in animals. In some embodiments, the invention is directed to peptides having bovine IGF-II biological activity, to production of such peptides, and to their use in effecting proliferation of certain cells (e.g. mammary epithelial or muscle) or in enhancing lactation in cattle or other animals.
摘要翻译：本发明涉及可用于促进动物生长和/或哺乳的新型肽，可用于生产这些肽的方法和DNA，以及利用这些肽促进动物生长或哺乳的方法。在一些实施方案中，本发明涉及具有牛IGF-II生物活性的肽，生产这样的肽，以及用于实现某些细胞（例如乳腺上皮或肌肉）的增殖或增强牛或其他动物的哺乳期。

9. 发明授权

US5399489A Production of proteins in procaryotes 失效
标题翻译：在原核生产蛋白质
公开(公告)号：US5399489A
公开(公告)日：1995-03-21
申请号：US975205
申请日：1992-11-12
申请人： Gwen G. Krivi
发明人： Gwen G. Krivi
IPC分类号： C12N15/00 , A23K1/165 , C07K14/61 , C12N15/18 , C12P21/02 , C12P21/00
CPC分类号： C07K14/61 , A23K20/168
摘要： A method for preparing polypeptides in bacteria with an alanine rather than a methionine at the N-terminus. The DNA sequence expressed has an alanine codon immediately following from one to about three contiguous methionine codons including a translation start signal and allows for the expression of polypeptides having the amino acid sequence of, for example, naturally occurring eucaryotic proteins such as various bovine and porcine somatotropin species.
摘要翻译：在N-末端用丙氨酸而不是甲硫氨酸在细菌中制备多肽的方法。所表达的DNA序列具有紧邻从一至约三个连续甲硫氨酸密码子（包括翻译起始信号）的丙氨酸密码子，并允许表达具有例如天然存在的真核生物蛋白如多种牛和猪的氨基酸序列的多肽生长激素种类。

10. 发明授权

US5130422A Variant somatotropin-encoding DNA 失效
标题翻译：变体生长激素编码DNA
公开(公告)号：US5130422A
公开(公告)日：1992-07-14
申请号：US743623
申请日：1991-08-12
申请人： Gwen G. Krivi , Michael R. Schlittler , Bernard N. Violand
发明人： Gwen G. Krivi , Michael R. Schlittler , Bernard N. Violand
IPC分类号： C07K14/61 , C12N15/18
CPC分类号： C07K14/61 , Y10S436/817
摘要： Variant somatotropins are described in which the asparagine located in the 95-101 region are replaced by glutamine which somatotropins exhibits enhanced stability.
摘要翻译：描述了变体生长激素，其中位于95-101区域中的天冬酰胺被谷氨酰胺代替，生长激素表现出增强的稳定性。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式