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1. 发明授权

US5506212A Oligonucleotides with substantially chirally pure phosphorothioate linkages 失效
标题翻译：具有基本上手性纯的硫代磷酸酯键的寡核苷酸
公开(公告)号：US5506212A
公开(公告)日：1996-04-09
申请号：US297703
申请日：1994-08-29
申请人： Glenn Hoke , Phillip D. Cook
发明人： Glenn Hoke , Phillip D. Cook
IPC分类号： A61K48/00 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C07J43/00 , C12N9/02 , C12N15/10 , C12N15/113 , C12P19/34 , C12Q1/68 , C12Q1/70 , A61K31/70
CPC分类号： A61K31/70 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C07J43/003 , C12N15/10 , C12N15/113 , C12N15/1137 , C12N9/0069 , C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6876 , C12Q1/701 , C12Y113/11012 , A61K48/00 , C12N2310/3125 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/33 , C12N2310/333 , C12N2310/336 , C12N2310/3521 , C12N2310/3523 , C12N2310/3527 , C12N2310/3531 , C12N2310/3533
摘要： Sequence specific phosphorothioate oligonucleotides comprising nucleoside units which are joined together by either substantially all Sp or substantially all Rp phosphorothioate intersugar linkages are provided. Such sequence specific phosphorothioate oligonucleotides having substantially chirally pure intersugar linkages are prepared by enzymatic synthesis from nucleoside 5'-O-(1-thiotriphosphates).
摘要翻译：提供了包含通过基本上所有Sp或基本上全部Rp硫代磷酸酯跨糖连接物连接在一起的核苷单元的序列特异性硫代磷酸酯寡核苷酸。通过从核苷5'-O-（1-硫代三磷酸酯）的酶合成制备具有基本上手性纯的间接连接的这种序列特异性硫代磷酸酯寡核苷酸。

2. 发明授权

US5620963A Oligonucleotides for modulating protein kinase C having phosphorothioate linkages of high chiral purity 失效
标题翻译：用于调节具有高手性纯度的硫代磷酸酯键的蛋白激酶C的寡核苷酸
公开(公告)号：US5620963A
公开(公告)日：1997-04-15
申请号：US468569
申请日：1995-06-06
申请人： Phillip D. Cook , Glenn Hoke
发明人： Phillip D. Cook , Glenn Hoke
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12N15/10 , C12N15/113 , C12P19/34 , C12Q1/68 , C12Q1/70 , A61K31/70
CPC分类号： A61K31/70 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C07J43/003 , C12N15/10 , C12N15/113 , C12N15/1131 , C12N15/1132 , C12N15/1133 , C12N15/1135 , C12N15/1137 , C12N9/0069 , C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6876 , C12Q1/701 , C12Y113/11012 , A61K48/00 , C12N2310/3125 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/33 , C12N2310/333 , C12N2310/336 , C12N2310/3521 , C12N2310/3523 , C12N2310/3527 , C12N2310/3531 , C12N2310/3533
摘要： Sequence-specific phosphorothioate oligonucleotides comprising nucleoside units which are joined together by either substantially all Sp or substantially all Rp phosphorothioate intersugar linkages are provided. Such sequence-specific phosphorothioate oligonucleotides having substantially chirally pure intersugar linkages are prepared by enzymatic or chemical synthesis. They are especially well suited as diagnostics, therapeutics and research reagents in instances of cancer mediated by PKC-.alpha..
摘要翻译：提供了包含通过基本上所有的Sp或基本上所有的Rp硫代磷酸酯杂合键连接在一起的核苷单元的序列特异性硫代磷酸酯寡核苷酸。通过酶促或化学合成来制备具有基本上手性纯粹的子宫颈间连接的这种序列特异性硫代磷酸酯寡核苷酸。它们特别适用于由PKC-α介导的癌症的诊断，治疗和研究试剂。

3. 发明授权

US5607923A Oligonucleotides for modulating cytomegalovirus having phosphorothioate linkages of high chiral purity 失效
标题翻译：用于调节具有高手性纯度的硫代磷酸酯键的巨细胞病毒的寡核苷酸
公开(公告)号：US5607923A
公开(公告)日：1997-03-04
申请号：US467597
申请日：1995-06-06
申请人： Phillip D. Cook , Glenn Hoke
发明人： Phillip D. Cook , Glenn Hoke
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12N15/10 , C12N15/113 , C12P19/34 , C12Q1/68 , C12Q1/70 , A61K31/70
CPC分类号： A61K31/70 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C07J43/003 , C12N15/10 , C12N15/113 , C12N15/1131 , C12N15/1132 , C12N15/1133 , C12N15/1135 , C12N15/1137 , C12N9/0069 , C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6876 , C12Q1/701 , C12Y113/11012 , A61K48/00 , C12N2310/3125 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/33 , C12N2310/333 , C12N2310/336 , C12N2310/3521 , C12N2310/3523 , C12N2310/3527 , C12N2310/3531 , C12N2310/3533 , Y10S514/912 , Y10S514/914
摘要： Sequence-specific phosphorothioate oligonucleotides comprising nucleoside units which are joined together by either substantially all Sp or substantially all Rp phosphorothioate intersugar linkages are provided. Such sequence-specific phosphorothioate oligonucleotides having substantially chirally pure intersugar linkages are prepared by enzymatic or chemical synthesis. They are especially well suited as diagnostics, therapeutics, and research reagents in instances of retinitis caused by cytomegalovirus.
摘要翻译：提供了包含通过基本上所有的Sp或基本上所有的Rp硫代磷酸酯杂合键连接在一起的核苷单元的序列特异性硫代磷酸酯寡核苷酸。通过酶促或化学合成来制备具有基本上手性纯粹的子宫颈间连接的这种序列特异性硫代磷酸酯寡核苷酸。它们特别适用于由巨细胞病毒引起的视网膜炎的诊断，治疗和研究试剂。

4. 发明授权

US5587361A Oligonucleotides having phosphorothioate linkages of high chiral purity 失效
标题翻译：具有高手性纯度的硫代磷酸酯键的寡核苷酸
公开(公告)号：US5587361A
公开(公告)日：1996-12-24
申请号：US469851
申请日：1995-06-06
申请人： Phillip D. Cook , Glenn Hoke
发明人： Phillip D. Cook , Glenn Hoke
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12N15/10 , C12N15/113 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： A61K31/70 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C07J43/003 , C12N15/10 , C12N15/113 , C12N15/1131 , C12N15/1132 , C12N15/1133 , C12N15/1135 , C12N15/1137 , C12N9/0069 , C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6876 , C12Q1/701 , C12Y113/11012 , A61K48/00 , C12N2310/3125 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/33 , C12N2310/333 , C12N2310/336 , C12N2310/3521 , C12N2310/3523 , C12N2310/3527 , C12N2310/3531 , C12N2310/3533
摘要： Sequence-specific phosphorothioate oligonucleotides comprising nucleoside units which are joined together by either substantially all Sp or substantially all Rp phosphorothioate intersugar linkages are provided. Such sequence-specific phosphorothioate oligonucleotides having substantially chirally pure intersugar linkages are prepared by enzymatic or chemical synthesis.
摘要翻译：提供了包含通过基本上所有的Sp或基本上所有的Rp硫代磷酸酯杂合键连接在一起的核苷单元的序列特异性硫代磷酸酯寡核苷酸。通过酶促或化学合成来制备具有基本上手性纯粹的子宫颈间连接的这种序列特异性硫代磷酸酯寡核苷酸。

5. 发明申请

US20050095606A1 Partially double-stranded nucleic acids, methods of making, and use thereof 审中-公开
标题翻译：部分双链核酸，制备方法和用途
公开(公告)号：US20050095606A1
公开(公告)日：2005-05-05
申请号：US10757468
申请日：2004-01-15
申请人： Glenn Hoke , John Hartwell , Adam Steel
发明人： Glenn Hoke , John Hartwell , Adam Steel
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6853 , C12Q2525/113 , C12Q2521/319 , C12Q2521/531 , C12Q2525/161 , C12Q2523/319 , C12Q2523/107
摘要： A process is disclosed for generating at least one partially double-stranded target nucleic acid, which contains at least one single-stranded region at a terminal end. The process comprises the steps of (a) providing at least one primer, P1, containing at least one labile nucleotide; (b) combining at least one target nucleic acid sequence with P1 to generate a double-stranded polynucleotide containing at least one labile nucleotide; (c) exposing the double-stranded polynucleotide to conditions that promote single-strand cleavage of the polynucleotide at the site of the at least one labile nucleotide of primer P1; and (d) exposing the cleaved polynucleotide to conditions that promote the dissociation of the cleaved portions of primer P1 from a terminal end. The labile nucleotide may be dUTP, wherein the single-stranded cleavage of the polynucleotide at the site of the labile nucleotide occurs by treatment with uracil N-glycosylase.
摘要翻译：公开了用于产生至少一个在末端含有至少一个单链区的部分双链靶核酸的方法。该方法包括以下步骤：（a）提供至少一种含有至少一个不稳定核苷酸的引物P1; （b）将至少一个靶核酸序列与P1结合以产生含有至少一个不稳定核苷酸的双链多核苷酸; （c）将双链多核苷酸暴露于在引物P1的至少一个不稳定核苷酸的位点处促进单链切割多核苷酸的条件; 和（d）将切割的多核苷酸暴露于促进引物P1的切割部分从终末端解离的条件。不稳定核苷酸可以是dUTP，其中在不稳定核苷酸位点处的多核苷酸的单链切割通过尿嘧啶N-糖基化酶的处理发生。

6. 发明申请

US20050170375A1 Methods for enhancing gene expression analysis 审中-公开
公开(公告)号：US20050170375A1
公开(公告)日：2005-08-04
申请号：US10948635
申请日：2004-09-24
申请人： Uwe Scherf , Glenn Hoke , Daniel Wilson , Debra Barnes
发明人： Uwe Scherf , Glenn Hoke , Daniel Wilson , Debra Barnes
IPC分类号： C12P19/34 , C12Q20060101 , C12Q1/68
CPC分类号： C12P19/34 , C12Q1/68 , C12Q1/6809 , C12Q2549/125 , C12Q2521/107
摘要： This application concerns improved methods of analyzing gene expression data where mRNA transcripts or representatives thereof that skew the gene expression profile of a cell or tissue sample are identified and removed from the population of mRNA transcripts prior to, during or subsequent to a reverse transcription reaction.

7. 发明授权

US06339066B1 Antisense oligonucleotides which have phosphorothioate linkages of high chiral purity and which modulate &bgr;I, &bgr;II, &ggr;, &dgr;, &Egr;, &zgr; and &eegr; isoforms of human protein kinase C 失效
标题翻译：具有高手性纯度的硫代磷酸酯键并且调节βI，βII，γ，δ和Egr;和zgr的反义寡核苷酸。和人类蛋白激酶C的同种异构体
公开(公告)号：US06339066B1
公开(公告)日：2002-01-15
申请号：US08829637
申请日：1997-03-31
申请人： C. Frank Bennett , Nicholas M. Dean , Phillip Dan Cook , Glenn Hoke
发明人： C. Frank Bennett , Nicholas M. Dean , Phillip Dan Cook , Glenn Hoke
IPC分类号： C07H2104
CPC分类号： A61K31/70 , A61K38/00 , A61K48/00 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C07J43/003 , C12N9/0069 , C12N9/12 , C12N15/10 , C12N15/113 , C12N15/1131 , C12N15/1132 , C12N15/1133 , C12N15/1135 , C12N15/1137 , C12N15/1138 , C12N2310/3125 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/33 , C12N2310/333 , C12N2310/336 , C12N2310/341 , C12N2310/346 , C12N2310/3521 , C12N2310/3523 , C12N2310/3527 , C12N2310/3531 , C12N2310/3533 , C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6876 , C12Q1/6883 , C12Q1/701 , C12Q2600/158 , C12Y113/11012 , C12Y207/11013
摘要： Compositions and methods are provided for the treatment and diagnosis of diseases associated with the expression of one or more of the &bgr;I, &bgr;II, &ggr;, &dgr;, &egr;, &zgr; or &eegr; isoforms (isozymes) of protein kinase C (PKC). Oligonucleotides are provided which are targeted to nucleic acids encoding PKC-&bgr;I, PKC-&bgr;II, PKC-&ggr;, PKC-&dgr;, PKC-&egr;, PKC-&zgr; or PKC-&eegr;. Provided herein are oligonucleotides specifically hybridizable with a translation initiation site, 5′-untranslated region, 3′-untranslated region or other targeted region of a &bgr;I, &bgr;II, &ggr;, &dgr;, &egr;, &zgr; or &eegr; isoform of PKC, wherein at least about 75% of the nucleoside units of a given oligonucleotide are joined together by a stereospecific (i.e., Sp or Rp) phosphorothioate 3′ to 5′ linkages. In preferred embodiments, the oligonucleotides of the disclosure additionally contain one or more chemical modifications. Also disclosed are methods of using the oligonucleotides of the invention for modulating the expression of at least one of the &bgr;I, &bgr;II, &ggr;, &dgr;, &egr;, &zgr; or &eegr; isoforms of PKC and for treating animals suffering from disease amenable to therapeutic intervention by modulating the expression of one or more of the &bgr;I, &bgr;II, &ggr;, &dgr;, &egr;, &zgr; or &eegr; isoforms of PKC.
摘要翻译：提供组合物和方法用于治疗和诊断与βI，βII，γ，δ，epsi和zgr中的一种或多种的表达相关的疾病。或蛋白激酶C（PKC）的eta同工型（同工酶）。提供寡核苷酸，其靶向编码PKC-βI，PKC-βII，PKC-γ，PKC-δ，PKC-epsi，PKC- 或PKC-eta。本文提供了与翻译起始位点，5'-非翻译区，3'非翻译区或βI，βII，γ，δ，ε1和zgr的其它目标区域特异性杂交的寡核苷酸。或等位基因型，其中给定寡核苷酸的至少约75％的核苷单位通过立体特异性（即Sp或Rp）硫代磷酸酯3'至5'键连接在一起。在优选的实施方案中，本公开的寡核苷酸另外含有一种或多种化学修饰。还公开了使用本发明的寡核苷酸调节βI，βII，γ，δ，epsi和zgr中至少一种的表达的方法。或eta同种型，并通过调节βI，βII，γ，δ，εi，和zgr中的一种或多种的表达来治疗患有适于治疗性干预的疾病的动物。或PKC的eta同种型。

8. 发明授权

US5654284A Oligonucleotides for modulating RAF kinase having phosphorothioate linkages of high chiral purity 失效
标题翻译：用于调节具有高手性纯度的硫代磷酸酯键的RAF激酶的寡核苷酸
公开(公告)号：US5654284A
公开(公告)日：1997-08-05
申请号：US466692
申请日：1995-06-06
申请人： Phillip Dan Cook , Glenn Hoke
发明人： Phillip Dan Cook , Glenn Hoke
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12N15/10 , C12N15/113 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： A61K31/70 , C07H19/10 , C07H19/20 , C07H21/00 , C12N15/10 , C12N15/113 , C12N15/1131 , C12N15/1132 , C12N15/1133 , C12N15/1135 , C12N15/1137 , C12P19/34 , C12Q1/6813 , C12Q1/6876 , C12Q1/701
摘要： Sequence-specific phosphorothioate oligonucleotides comprising nucleoside units which are joined together by either substantially all Sp or substantially all Rp phosphorothioate intersugar linkages are provided. Such sequence-specific phosphorothioate oligonucleotides having substantially chirally pure intersugar linkages are prepared by enzymatic or chemical synthesis.
摘要翻译：提供了包含通过基本上所有的Sp或基本上所有的Rp硫代磷酸酯杂合键连接在一起的核苷单元的序列特异性硫代磷酸酯寡核苷酸。通过酶促或化学合成来制备具有基本上手性纯粹的子宫颈间连接的这种序列特异性硫代磷酸酯寡核苷酸。

9. 发明授权

US5635488A Compounds having phosphorodithioate linkages of high chiral purity 失效
标题翻译：具有高手性纯度的二硫代磷酸酯键的化合物
公开(公告)号：US5635488A
公开(公告)日：1997-06-03
申请号：US470129
申请日：1995-06-06
申请人： Phillip D. Cook , Glenn Hoke
发明人： Phillip D. Cook , Glenn Hoke
IPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12N15/10 , C12N15/113 , C12P19/34 , C12Q1/68 , C12Q1/70 , A61K31/70
CPC分类号： A61K31/70 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C07J43/003 , C12N15/10 , C12N15/113 , C12N15/1131 , C12N15/1132 , C12N15/1133 , C12N15/1135 , C12N15/1137 , C12N9/0069 , C12P19/34 , C12Q1/68 , C12Q1/6813 , C12Q1/6876 , C12Q1/701 , C12Y113/11012 , A61K48/00 , C12N2310/3125 , C12N2310/315 , C12N2310/32 , C12N2310/321 , C12N2310/322 , C12N2310/33 , C12N2310/333 , C12N2310/336 , C12N2310/3521 , C12N2310/3523 , C12N2310/3527 , C12N2310/3531 , C12N2310/3533
摘要： Sequence-specific phosphorothioate oligonucleotides comprising nucleoside units which are joined together by either substantially all Sp or substantially all Rp phosphorothioate intersugar linkages are provided. Such sequence-specific phosphorothioate oligonucleotides having substantially chirally pure intersugar linkages are prepared by enzymatic or chemical synthesis. Sequence-specific phosphorodithioate oligonucleotides are also provided. Such sequence-specific phosphorodithioate oligonucleotides are prepared by chemical synthesis. They are especially well suited as diagnostics, therapeutics and research reagents.
摘要翻译：提供了包含通过基本上所有的Sp或基本上所有的Rp硫代磷酸酯杂合键连接在一起的核苷单元的序列特异性硫代磷酸酯寡核苷酸。通过酶促或化学合成来制备具有基本上手性纯粹的子宫颈间连接的这种序列特异性硫代磷酸酯寡核苷酸。还提供了序列特异性二硫代磷酸酯寡核苷酸。这种序列特异性二硫代磷酸酯寡核苷酸通过化学合成制备。它们特别适用于诊断，治疗和研究试剂。

10. 发明申请

US20050221310A1 Methods for enhancing gene expression analysis 审中-公开
标题翻译：增强基因表达分析的方法
公开(公告)号：US20050221310A1
公开(公告)日：2005-10-06
申请号：US10504072
申请日：2004-06-04
申请人： Uwe Scherf , Glenn Hoke , Daniel Wilson , Debra Barnes
发明人： Uwe Scherf , Glenn Hoke , Daniel Wilson , Debra Barnes
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6809 , C12Q2549/125 , C12Q2521/107
摘要： This application concerns improved methods of analyzing gene expression data where mRNA transcripts or representatives thereof that skew the gene expression profile of a cell or tissue sample are identified and removed from the population of mRNA transcripts prior to, during or subsequent to a reverse transcription reaction.
摘要翻译：该应用涉及分析基因表达数据的改进方法，其中在逆转录反应之前，期间或之后，从mRNA转录物群体中鉴定出偏倚细胞或组织样品的基因表达谱的mRNA转录物或其代表。

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