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1. 发明授权

US08846309B2 Methods for detecting Lp-PLA2 activity and inhibition of Lp-PLA2 activity 有权
标题翻译：检测Lp-PLA2活性和抑制Lp-PLA2活性的方法
公开(公告)号：US08846309B2
公开(公告)日：2014-09-30
申请号：US12817677
申请日：2010-06-17
申请人： Yaping Shou , Yin-Fai Siu , George T. Walker
发明人： Yaping Shou , Yin-Fai Siu , George T. Walker
IPC分类号： C12Q1/00 , C12Q1/61 , C12Q1/34 , C12Q1/44
CPC分类号： C12Q1/61 , C12Q1/34 , C12Q1/44 , C12Q2334/10 , G01N2333/918
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
摘要翻译：本发明涉及用于测定来自动物的至少一种样品中Lp-PLA2的活性的方法。本发明还涉及用于测定来自施用Lp-PLA2抑制剂的动物的样品中Lp-PLA2活性的抑制的方法。

2. 发明申请

US20100256919A1 METHODS FOR DETECTING LP-PLA2 ACTIVITY AND INHIBITION OF LP-PLA2 ACTIVITY 有权
标题翻译：检测LP-PLA2活性和抑制LP-PLA2活性的方法
公开(公告)号：US20100256919A1
公开(公告)日：2010-10-07
申请号：US12817677
申请日：2010-06-17
申请人： Yaping Shou , Yin-Fai Siu , George T. Walker
发明人： Yaping Shou , Yin-Fai Siu , George T. Walker
IPC分类号： G01N33/53 , G06F19/00
CPC分类号： C12Q1/61 , C12Q1/34 , C12Q1/44 , C12Q2334/10 , G01N2333/918
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
摘要翻译：本发明涉及用于测定来自动物的至少一种样品中Lp-PLA2的活性的方法。本发明还涉及用于测定来自施用Lp-PLA2抑制剂的动物的样品中Lp-PLA2活性的抑制的方法。

3. 发明授权

US5624825A Simultaneous amplification of multiple targets 失效
标题翻译：同时放大多个目标
公开(公告)号：US5624825A
公开(公告)日：1997-04-29
申请号：US451313
申请日：1995-05-26
申请人： George T. Walker , James G. Nadeau , Michael C. Little
发明人： George T. Walker , James G. Nadeau , Michael C. Little
IPC分类号： G01N33/566 , C07H21/04 , C12N15/09 , C12P19/34 , C12Q1/68 , G11B5/00 , G11B5/09 , G11B5/187 , G11B5/31 , G11B5/455 , G11B5/465 , G11B19/04
CPC分类号： C12Q1/6853
摘要： Methods for multiplex amplification of target nucleic acid sequences using a single pair of primers. Defined sequences are appended to the ends of multiple target sequences as part of the amplification reaction so that no steps in addition to amplification are required. The target sequences with the appended defined sequences need not be isolated prior to amplification. In one embodiment for coamplification of two target sequences, a sequence corresponding to a terminal segment of the first target sequence is appended to one end of the second target sequence and a sequence corresponding to a terminal segment of the second target sequence is appended to one end of the first target sequence. Amplification of the two targets then requires only a single pair of primers. Alternatively, a single defined sequence may be appended to the 5' and 3' ends of any number of selected targets. All such modified target sequences may then be amplified using a single pair of primers which hybridize to the defined end-sequences.
摘要翻译：使用单对引物多重扩增靶核酸序列的方法。定义的序列作为扩增反应的一部分附加到多个靶序列的末端，因此不需要扩增步骤。具有附加定义序列的靶序列不需要在扩增前分离。在两个目标序列的共放大的一个实施例中，对应于第一目标序列的末端片段的序列被附加到第二目标序列的一端，并且与第二目标序列的末端片段相对应的序列被附加到一端的第一个靶序列。两个靶标的扩增只需要一对引物即可。或者，单个定义的序列可以附加到任意数目的所选目标的5'和3'端。然后可以使用与定义的末端序列杂交的单对引物扩增所有这些修饰的靶序列。

4. 发明授权

US5270184A Nucleic acid target generation 失效
标题翻译：核酸靶产生
公开(公告)号：US5270184A
公开(公告)日：1993-12-14
申请号：US794399
申请日：1991-11-19
申请人： George T. Walker , Michael C. Little , James G. Nadeau
发明人： George T. Walker , Michael C. Little , James G. Nadeau
IPC分类号： C12N15/02 , C12N15/09 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6846 , C12Q1/6853
摘要： The invention is a method for generating nucleic acid sequences ends which comprises;(a) hybridizing a primer to a nucleic acid sequence,(b) hybridizing a primer to the nucleic acid sequence in (a) located 5' to the primer in (a), and(c) polymerizing both primers so that the primer in (a) is displaced from the nucleic acid sequence.The invention provides a method for generating target nucleic acid sequences for subsequent amplification. The method is applicable to both DNA and RNA.

5. 发明申请

US20120276569A1 METHODS FOR DETECTING LP-PLA2 ACTIVITY AND INHIBITION OF LP-PLA2 ACTIVITY 有权
标题翻译：检测LP-PLA2活性和抑制LP-PLA2活性的方法
公开(公告)号：US20120276569A1
公开(公告)日：2012-11-01
申请号：US13540571
申请日：2012-07-02
申请人： Yaping Shou , Yin-Fai SIU , George T. WALKER
发明人： Yaping Shou , Yin-Fai SIU , George T. WALKER
IPC分类号： G01N21/25
CPC分类号： C12Q1/61 , C12Q1/34 , C12Q1/44 , C12Q2334/10 , G01N2333/918
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
摘要翻译：本发明涉及用于测定来自动物的至少一种样品中Lp-PLA2的活性的方法。本发明还涉及用于测定来自施用Lp-PLA2抑制剂的动物的样品中Lp-PLA2活性的抑制的方法。

6. 发明申请

US20100196908A1 SYSTEMS AND METHODS FOR DETECTING A SIGNAL AND APPLYING THERMAL ENERGY TO A SIGNAL TRANSMISSION ELEMENT 有权
标题翻译：用于检测信号并将热能应用于信号传输元件的系统和方法
公开(公告)号：US20100196908A1
公开(公告)日：2010-08-05
申请号：US12696406
申请日：2010-01-29
申请人： David OPALSKY , George T. WALKER , Norman C. NELSON , Richard S. LEE , Sara H. FAN
发明人： David OPALSKY , George T. WALKER , Norman C. NELSON , Richard S. LEE , Sara H. FAN
IPC分类号： C12Q1/68 , G01N21/64 , H01L31/09 , H01L31/0232 , C12M1/34
CPC分类号： G01N21/03 , B01L3/5027 , B01L3/505 , B01L7/52 , B01L9/527 , B01L2200/04 , B01L2200/146 , B01L2300/021 , B01L2300/044 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/087 , B01L2300/1827 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0666 , G01N21/0332 , G01N2021/0328 , G01N2021/0346 , G01N2035/00346
摘要： A signal detection system configured for detecting a signal emitted by the contents of a reaction receptacle is also configured to apply thermal energy to a portion of the reaction receptacle to affect a reaction occurring within the reaction receptacle. More particularly, a system for detecting electromagnetic radiation emitted by the contents of a reaction receptacle includes a transmission element configured for transmitting electromagnetic radiation from the contents of the receptacle, a thermal element associated with the transmission element and configured to apply thermal energy to at least a portion of the receptacle, and a detector configured to receive electromagnetic radiation from the transmission element and to generate a signal corresponding to a characteristic of the electromagnetic radiation received by the detector.
摘要翻译：被配置为检测由反应容器的内容物发射的信号的信号检测系统还被配置为将热能施加到反应容器的一部分以影响在反应容器内发生的反应。更具体地，用于检测由反应容器的内容物发射的电磁辐射的系统包括传输元件，其被配置用于从容器的内容物传输电磁辐射，与传输元件相关联的热元件，并被配置为将热能施加到至少所述插座的一部分以及被配置为从所述传输元件接收电磁辐射并且产生对应于由所述检测器接收的电磁辐射的特性的信号的检测器。

7. 发明授权

US5455166A Strand displacement amplification 失效
标题翻译：绞线位移放大
公开(公告)号：US5455166A
公开(公告)日：1995-10-03
申请号：US819358
申请日：1992-01-09
申请人： George T. Walker
发明人： George T. Walker
IPC分类号： C12N15/09 , C12N15/10 , C12P19/34 , C12Q1/28 , C12Q1/42 , C12Q1/44 , C12Q1/48 , C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6853
摘要： This invention relates a nucleic acid target amplification and detection method which operates at a single temperature and makes use of a polymerase in conjunction with an endonuclease that will nick the polymerized strand such that the polymerase will displace the strand without digestion while generating a newly polymerized strand.
摘要翻译：本发明涉及在单一温度下操作的核酸靶扩增和检测方法，并利用聚合酶与内切核酸酶结合使用聚合物链，使得聚合酶将在不消化的同时置换链，同时产生新聚合的链。

8. 发明授权

US5422252A Simultaneous amplification of multiple targets 失效
标题翻译：同时放大多个目标
公开(公告)号：US5422252A
公开(公告)日：1995-06-06
申请号：US73197
申请日：1993-06-04
申请人： George T. Walker , James G. Nadeau , Michael C. Little
发明人： George T. Walker , James G. Nadeau , Michael C. Little
IPC分类号： G01N33/566 , C07H21/04 , C12N15/09 , C12P19/34 , C12Q1/68 , G11B5/00 , G11B5/09 , G11B5/187 , G11B5/31 , G11B5/455 , G11B5/465 , G11B19/04 , C12Q1/70
CPC分类号： C12Q1/6853
摘要： Methods for multiplex amplification of target nucleic acid sequences using a single pair of primers. Defined sequences are appended to the ends of multiple target sequences as part of the amplification reaction so that no steps in addition to amplification are required. The target sequences with the appended defined sequences need not be isolated prior to amplification. In one embodiment for coamplification of two target sequences, a sequence corresponding to a terminal segment of the first target sequence is appended to one end of the second target sequence and a sequence corresponding to a terminal segment of the second target sequence is appended to one end of the first target sequence. Amplification of the two targets then requires only a single pair of primers. Alternatively, a single defined sequence may be appended to the 5' and 3' ends of any number of selected targets. All such modified target sequences may then be amplified using a single pair of primers which hybridize to the defined end-sequences.
摘要翻译：使用单对引物多重扩增靶核酸序列的方法。定义的序列作为扩增反应的一部分附加到多个靶序列的末端，因此不需要扩增步骤。具有附加定义序列的靶序列不需要在扩增前分离。在两个目标序列的共放大的一个实施例中，对应于第一目标序列的末端片段的序列被附加到第二目标序列的一端，并且与第二目标序列的末端片段相对应的序列被附加到一端的第一个靶序列。两个靶标的扩增只需要一对引物即可。或者，单个定义的序列可以附加到任意数目的所选目标的5'和3'端。然后可以使用与定义的末端序列杂交的单对引物扩增所有这些修饰的靶序列。

9. 发明授权

US5207667A One hand IV needle injection site connector 失效
标题翻译：单针IV针注射部位连接器
公开(公告)号：US5207667A
公开(公告)日：1993-05-04
申请号：US768374
申请日：1991-09-27
申请人： George T. Walker , James M. Verespej
发明人： George T. Walker , James M. Verespej
IPC分类号： A61M39/04
CPC分类号： A61M39/04
摘要： A protected needle coupling for connecting to a conventional IV injection site comprises an elongated tubular housing having a proximal end and a distal end, a needle mounted at the proximal end and extending coaxially toward the distal end to a protected position within the housing, a connector at the proximal end for connecting to an IV tubing, and a telescoping tubular member reciprocally mounted in said housing and having axially extending slits defining a pair of opposed semi-cylindrical fingers extendable from the distal end of the housing, and having inwardly directed shoulders at the outer ends thereof for engaging a shoulder of an IV injection port for pulling the injection port into the housing for coupling engagement with the needle therein, and a tab extending outward from the telescoping member for manually forcing the telescoping member into the housing.
摘要翻译：用于连接到常规IV注射部位的保护针联接件包括具有近端和远端的细长管状壳体，安装在近端并且朝向远端同轴地延伸到壳体内的受保护位置的针，连接器在连接到IV管的近端处，以及可往复安装在所述壳体中的伸缩管状构件，并且具有限定一对相对的半圆柱形指状物的轴向延伸狭缝，所述相对的半圆柱形指状物可从壳体的远端延伸，并且具有向内指向的肩部其外端用于接合IV注射口的肩部，用于将注射口拉入壳体中，用于与针头相配合，以及从伸缩构件向外延伸的突出部，用于手动将伸缩构件推入壳体。

10. 发明申请

US20150017671A1 METHODS FOR DETECTING LP-PLA2 ACTIVITY AND INHIBITION OF LP-PLA2 ACTIVITY 审中-公开
公开(公告)号：US20150017671A1
公开(公告)日：2015-01-15
申请号：US14498980
申请日：2014-09-26
申请人： Yaping SHOU , Yin-Fai SIU , George T. WALKER
发明人： Yaping SHOU , Yin-Fai SIU , George T. WALKER
IPC分类号： C12Q1/44
CPC分类号： G01N33/573 , C12Q1/44 , C12Q2334/10 , G01N33/581 , G01N2333/918 , G01N2500/02 , G01N2500/04 , G01N2500/20
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.

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