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1. 发明申请

US20060110793A1 Methods and compositions for increasing longevity and protein yield from a cell culture 有权
公开(公告)号：US20060110793A1
公开(公告)日：2006-05-25
申请号：US11187863
申请日：2005-07-25
申请人： David Goldenberg , Zhengxing Qu , Eva Horak , Ivan Horak , Chien Chang , Edmund Rossi , Jeng-Dar Yang
发明人： David Goldenberg , Zhengxing Qu , Eva Horak , Ivan Horak , Chien Chang , Edmund Rossi , Jeng-Dar Yang
IPC分类号： C07H21/04 , C12P21/06 , C12N9/00 , C07K14/52 , C07K14/54 , C07K14/56 , C07K14/475 , C07K16/18 , C07K14/505
CPC分类号： C07K16/00 , A61K2039/505 , C07K14/4747 , C07K16/18 , C07K16/2803 , C07K16/3007 , C07K2317/24 , C07K2317/31 , C12N5/0694 , C12N2500/90 , C12N2501/48 , C12N2510/02 , C12P21/02
摘要： Disclosed herein are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. By transfecting cells in culture with an apoptosis-inhibiting gene or vector, cells in culture can survive longer, resulting in extension of the state and yield of protein biosynthesis. Expression of the apoptosis-inhibitor within the cells, because it does not kill the cells, allows the cells, or an increased fraction thereof, to be maintained in culture for longer periods. This invention then allows for controlled, enhanced protein production of cell lines for commercial and research uses, particularly the enhanced production of growth factors, interferons, interleukins, hormones, enzymes, and monoclonal antibodies, and the like. The method preferentially involves eukaryotic cells in culture, and more advantageously mammalian cells in culture.

2. 发明申请

US20060099205A1 Bispecific single chain FV antibody molecules and methods of use thereof 有权
标题翻译：双特异性单链FV抗体分子及其使用方法
公开(公告)号：US20060099205A1
公开(公告)日：2006-05-11
申请号：US11154103
申请日：2005-06-15
申请人： Gregory Adams , Eva Horak , Louis Weiner , James Marks
发明人： Gregory Adams , Eva Horak , Louis Weiner , James Marks
IPC分类号： A61K39/395 , C07K16/44 , C07K16/28
CPC分类号： A61K39/395 , A61K51/109 , A61K2039/505 , C07K16/2863 , C07K16/32 , C07K2317/31 , C07K2317/622 , G01N2333/71
摘要： Bispecific single chain antibody molecules are disclosed which may be used to advantage to treat various forms of cancer associated with the overexpression of members of the EGFR protein family.
摘要翻译：公开了双特异性单链抗体分子，其可用于治疗与EGFR蛋白家族的成员的过表达相关的各种形式的癌症。

3. 发明授权

US07531327B2 Methods and compositions for increasing longevity and protein yield from a cell culture 有权
标题翻译：用于增加细胞培养物的寿命和蛋白质产量的方法和组合物
公开(公告)号：US07531327B2
公开(公告)日：2009-05-12
申请号：US11187863
申请日：2005-07-25
申请人： David M. Goldenberg , Zhengxing Qu , Eva Horak , Ivan D. Horak , Chien Hsing Chang , Edmund A. Rossi , Jeng-Dar Yang
发明人： David M. Goldenberg , Zhengxing Qu , Eva Horak , Ivan D. Horak , Chien Hsing Chang , Edmund A. Rossi , Jeng-Dar Yang
IPC分类号： C12N5/10 , C12N5/16
CPC分类号： C07K16/00 , A61K2039/505 , C07K14/4747 , C07K16/18 , C07K16/2803 , C07K16/3007 , C07K2317/24 , C07K2317/31 , C12N5/0694 , C12N2500/90 , C12N2501/48 , C12N2510/02 , C12P21/02
摘要： Disclosed herein are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. By transfecting cells in culture with an apoptosis-inhibiting gene or vector, cells in culture can survive longer, resulting in extension of the state and yield of protein biosynthesis. Expression of the apoptosis-inhibitor within the cells, because it does not kill the cells, allows the cells, or an increased fraction thereof, to be maintained in culture for longer periods. This invention then allows for controlled, enhanced protein production of cell lines for commercial and research uses, particularly the enhanced production of growth factors, interferons, interleukins, hormones, enzymes, and monoclonal antibodies, and the like. The method preferentially involves eukaryotic cells in culture, and more advantageously mammalian cells in culture.
摘要翻译：本文公开了用于增加细胞培养物的寿命并允许增加蛋白质生产的组合物和方法，优选重组蛋白，例如抗体，肽，酶，生长因子，白细胞介素，干扰素，激素和疫苗。通过用细胞凋亡抑制基因或载体转染培养细胞，培养中的细胞可以长时间存活，导致蛋白质生物合成的状态和产量的延长。凋亡抑制剂在细胞内的表达，因为它不杀死细胞，允许细胞或其增加的部分在培养物中保持较长时间。本发明然后允许用于商业和研究用途的细胞系的受控增强的蛋白质生产，特别是生长因子，干扰素，白细胞介素，激素，酶和单克隆抗体的增强生产等。该方法优先涉及培养中的真核细胞，更有利于培养中的哺乳动物细胞。

4. 发明申请

US20070092947A1 Methods and compositions for increasing longevity and protein yield from a cell culture 审中-公开
标题翻译：用于增加细胞培养物的寿命和蛋白质产量的方法和组合物
公开(公告)号：US20070092947A1
公开(公告)日：2007-04-26
申请号：US11253666
申请日：2005-10-20
申请人： David Goldenberg , Zhengxing Qu , Eva Horak , Ivan Horak , Chien Chang , Edmund Rossi , Jeng-Dar Yang
发明人： David Goldenberg , Zhengxing Qu , Eva Horak , Ivan Horak , Chien Chang , Edmund Rossi , Jeng-Dar Yang
IPC分类号： C07H21/04 , C12P21/02 , C07K14/53 , C07K14/54 , C07K14/56 , C12N5/06 , C12N5/08
CPC分类号： C12P21/02 , C12N2510/02
摘要： Disclosed herein are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. By transfecting cells in culture with an apoptosis-inhibiting gene or vector, cells in culture can survive longer, resulting in extension of the state and yield of protein biosynthesis. Expression of the apoptosis-inhibitor within the cells, because it does not kill the cells, allows the cells, or an increased fraction thereof, to be maintained in culture for longer periods. This invention then allows for controlled, enhanced protein production of cell lines for commercial and research uses, particularly the enhanced production of growth factors, interferons, interleukins, hormones, enzymes, and monoclonal antibodies, and the like. The method preferentially involves eukaryotic cells in culture, and more advantageously mammalian cells in culture.
摘要翻译：本文公开了用于增加细胞培养物的寿命并允许增加蛋白质生产的组合物和方法，优选重组蛋白，例如抗体，肽，酶，生长因子，白细胞介素，干扰素，激素和疫苗。通过用细胞凋亡抑制基因或载体转染培养细胞，培养中的细胞可以长时间存活，导致蛋白质生物合成的状态和产量的延长。凋亡抑制剂在细胞内的表达，因为它不杀死细胞，允许细胞或其增加的部分在培养物中保持较长时间。本发明然后允许用于商业和研究用途的细胞系的受控增强的蛋白质生产，特别是生长因子，干扰素，白细胞介素，激素，酶和单克隆抗体的增强生产等。该方法优先涉及培养中的真核细胞，更有利于培养中的哺乳动物细胞。

5. 发明申请

US20050079184A1 Bispecific antibodies for inducing apoptosis of tumor and diseased cells 审中-公开
标题翻译：用于诱导肿瘤和患病细胞凋亡的双特异性抗体
公开(公告)号：US20050079184A1
公开(公告)日：2005-04-14
申请号：US10913509
申请日：2004-08-09
申请人： Chien Hsing-Chang , David Goldenberg , Hans Hansen , Eva Horak , Ivan Horak
发明人： Chien Hsing-Chang , David Goldenberg , Hans Hansen , Eva Horak , Ivan Horak
IPC分类号： A61K39/395 , C07K20060101 , C07K16/28 , C07K16/30 , C07K16/46 , G01N33/574
CPC分类号： C07K16/2887 , C07K16/2803 , C07K2317/31 , C07K2317/55 , C07K2317/73 , C07K2317/75
摘要： The bispecific antibodies of the present invention are in the form of a heteroconjugate that inhibits growth and induces apoptosis of a diseased cell and that does not require the recruitment of effector cells. The heteroconjugate has at least two binding arms wherein each of the binding arms possesses a different specificity and need not have apoptotic activity when not conjugated to each other. Also provided are methods of treating and diagnosing a diseased cell using the bispecfic antibodies of the present invention.
摘要翻译：本发明的双特异性抗体是异源结合物的形式，其抑制生长并诱导病变细胞的凋亡，并且不需要募集效应细胞。杂合物具有至少两个结合臂，其中每个结合臂具有不同的特异性，并且当不彼此结合时不需要具有凋亡活性。还提供了使用本发明的双特异性抗体治疗和诊断患病细胞的方法。

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