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1. 发明授权

US5196323A Process for preparing and purifying alpha-interferon 失效
标题翻译：制备和纯化α-干扰素的方法
公开(公告)号：US5196323A
公开(公告)日：1993-03-23
申请号：US713618
申请日：1991-06-12
申请人： Gerhard Bodo , Ingrid Maurer-Fogy , Edgar Falkner , Silvia J. Lindner
发明人： Gerhard Bodo , Ingrid Maurer-Fogy , Edgar Falkner , Silvia J. Lindner
IPC分类号： A61K38/00 , C07K14/56
CPC分类号： C07K14/56 , A61K38/00
摘要： A process for the preparation and purification of recombinant Interferon-.alpha. is disclosed. The invention is directed to a process comprising the following steps: cultivating E. coli containing the interferon gene for a growth period during which not more than 5% methionine-interferon is formed; extracting and concentrating the expressed interferon; subjecting the preliminarily purified material to Tandem Chromatography, wherein the Tandem Chromatography comprises separation on a cellulose column followed by an anti-alpha-interferon monoclonal antibody affinity column; subjecting the thus purified material to isoelectric precipitation of impurities at about pH 4.0 to about pH 4.8; and purifying the interferon by chromatography on a high performance cation exchange column using a volatile buffer, wherein the purified interferon is non-immunogenic when administered parenterally to a human.
摘要翻译：公开了重组干扰素-α的制备和纯化方法。本发明涉及一种包括以下步骤的方法：培养含有干扰素基因的大肠杆菌生长期，其中不超过5％的甲硫氨酸 - 干扰素形成; 提取和浓缩表达的干扰素; 将预先纯化的材料进行串联层析，其中串联色谱法包括在纤维素柱上分离，随后是抗α-干扰素单克隆抗体亲和柱; 使如此纯化的材料经受大约pH 4.0至约pH 4.8的杂质的等电沉淀; 并使用挥发性缓冲液在高性能阳离子交换柱上通过色谱法纯化干扰素，其中当肠胃外给予人时，纯化的干扰素是非免疫原性的。

2. 发明授权

US4917887A Hybrid interferons, their use as pharmaceutical compositions and as intermediate products for the preparation of antibodies and the use thereof and processes for preparing them 失效
标题翻译：混合干扰素，它们作为药物组合物的用途以及用于制备抗体的中间产物及其用途及其制备方法
公开(公告)号：US4917887A
公开(公告)日：1990-04-17
申请号：US23634
申请日：1987-03-09
申请人： Rudolf Hauptmann , Peter Swetly , Peter Meindl , Gunther Adolf , Edgar Falkner , Gerhard Bodo , Ingrid Maurer-Fogy
发明人： Rudolf Hauptmann , Peter Swetly , Peter Meindl , Gunther Adolf , Edgar Falkner , Gerhard Bodo , Ingrid Maurer-Fogy
IPC分类号： C07K14/005 , A61K38/00 , A61K38/21 , C07H21/04 , C07K1/22 , C07K14/00 , C07K14/195 , C07K14/52 , C07K14/555 , C07K14/56 , C07K16/00 , C07K16/24 , C07K17/10 , C12N1/20 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/20 , C12N15/70 , C12N15/81 , C12P21/00 , C12P21/02 , C12R1/125 , C12R1/19 , C12R1/38 , C12R1/425 , C12R1/91
CPC分类号： C12N15/70 , C07K14/555 , C07K14/56 , C07K16/249 , C12N15/81 , A61K38/00
摘要： This invention relates to new hybrid interferons consisting of part of an .alpha.-interferon and part of an omega interferon, the N-terminal Met or N-formyl-Met derivatives thereof and, if the peptide sequence of the hybrid interferon contains a glycosylation site, the N-glycosylated derivatives thereof, their use as pharmaceutical compositions and as intermediate products for immunizing experimental animals and processes for producing them,new monoclonal antibodies and their use in purifying .alpha. and omega-interferons, the hybrid cell lines which secrete them and processes for preparing them,a new process for purifying .alpha. and omega-interferons by means of a new antibody affinity column containing the above-mentioned new monoclonal antibodies, and processes for the preparation thereof,new hybrid plasmids for improving the expression of omega-interferons and new intermediate plasmids for preparing the new plasmids and processes for the preparation thereof.
摘要翻译：本发明涉及由α-干扰素和ω-干扰素的一部分组成的新的杂合干扰素，其N-末端Met或N-甲酰基-Mc衍生物，如果杂合干扰素的肽序列含有糖基化位点，其N-糖基化衍生物，它们作为药物组合物的用途和用于免疫实验动物的中间产物及其制备方法，新的单克隆抗体及其在纯化α和ω-干扰素中的用途，分泌它们的杂交细胞系和制备它们，通过含有上述新单克隆抗体的新抗体亲和柱纯化α和ω-干扰素的新方法及其制备方法，用于改善ω-干扰素表达的新型杂合质粒和新的用于制备新质粒的中间质粒及其制备方法。

3. 发明授权

US5837842A Vascular anticoagulant proteins DNA which codes them, processer for preparing them and their use 失效
标题翻译：编码它们的血管抗凝蛋白DNA，用于制备它们的处理器及其用途
公开(公告)号：US5837842A
公开(公告)日：1998-11-17
申请号：US376050
申请日：1995-01-23
申请人： Rudolf Hauptmann , Ingrid Maurer-Fogy , Gerhard Bodo , Peter Swetly , Christian Stratowa , Edgar Falkner , Gunther Adolf , Christiaan Maria Peter Reutelingsperger
发明人： Rudolf Hauptmann , Ingrid Maurer-Fogy , Gerhard Bodo , Peter Swetly , Christian Stratowa , Edgar Falkner , Gunther Adolf , Christiaan Maria Peter Reutelingsperger
IPC分类号： G01N33/50 , A61K35/14 , A61K38/00 , A61P7/02 , A61P29/00 , C07K1/20 , C07K14/00 , C07K14/47 , C07K16/00 , C07K16/18 , C07K19/00 , C12N1/20 , C12N5/10 , C12N15/09 , C12N15/70 , C12P21/02 , C12R1/19 , C12R1/91 , G01N33/86 , C07H21/04
CPC分类号： C12N15/70 , C07K14/4721 , C07K16/18 , G01N33/86 , A61K38/00
摘要： This invention relates to biologically active proteins, the DNA molecules coding for them, processes for preparing them and their use.
摘要翻译：本发明涉及生物活性蛋白质，编码它们的DNA分子，其制备方法及其应用。

4. 发明授权

US5710027A Process and vector for expressing alpha-interferon in E. coli 失效
标题翻译：用于在大肠杆菌中表达α-干扰素的方法和载体
公开(公告)号：US5710027A
公开(公告)日：1998-01-20
申请号：US249671
申请日：1994-05-26
申请人： Rudolf Hauptmann , Edgar Falkner , Gerhard Bodo , Tilman Voss , Ingrid Maurer-Fogy
发明人： Rudolf Hauptmann , Edgar Falkner , Gerhard Bodo , Tilman Voss , Ingrid Maurer-Fogy
IPC分类号： C12N15/09 , C07K1/18 , C07K1/20 , C07K1/22 , C07K14/245 , C07K14/52 , C07K14/555 , C07K14/56 , C12N15/21 , C12N15/70 , C12P21/02 , C12R1/19
CPC分类号： C12N15/70 , C07K14/245 , C07K14/56 , C07K2319/02
摘要： Methods and vectors for expressing interferon-alpha (IFN-.alpha.) proteins in E. coli are provided. Use of a vector comprising an IFN-.alpha. sequence fused to an E. coli heat-stable enterotoxin signal sequence (STII) under the control of the E. coli phosphatase (phoA) promoter affords high levels of correctly-folded and -processed, biologically active IFN-.alpha. polypeptides.
摘要翻译：提供了用于在大肠杆菌中表达干扰素-α（IFN-α）蛋白的方法和载体。在大肠杆菌磷酸酶（phoA）启动子控制下，包含与大肠杆菌热稳定肠毒素信号序列（STII）融合的IFN-α序列的载体的使用提供了高水平的正确折叠和加工的生物学活性IFN-α多肽。

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