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1. 发明授权

US06503715B1 Nucleic acid ligand diagnostic biochip 有权
标题翻译：核酸配体诊断生物芯片
公开(公告)号：US06503715B1
公开(公告)日：2003-01-07
申请号：US09723394
申请日：2000-11-28
申请人： Larry Gold , Dan Drolet , Dom Zichi , Sumedha Jayasena , Steve Creighton , Stanley Gill
发明人： Larry Gold , Dan Drolet , Dom Zichi , Sumedha Jayasena , Steve Creighton , Stanley Gill
IPC分类号： C12Q168
CPC分类号： C12Q1/6839 , C07B2200/11 , C12Q1/6834 , C12Q1/6837 , C40B40/00 , Y10S977/924 , C12Q2565/514 , C12Q2565/101 , C12Q2541/101 , C12Q2525/205
摘要： A Nucleic acid ligand “Biochip” is disclosed, consisting of a solid support to which one or more specific Nucleic acid ligands is attached in a spatially defined manner. Each Nucleic acid ligand binds specifically and avidly to a particular Target molecule contained within a Test mixture, such as a Bodily fluid. The Target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. Contacting the Test mixture with the Biochip leads to the binding of a Target molecule to its cognate Nucleic acid ligand. Binding of Target to the Nucleic acid ligand results in a detectable change at each specific location on the Biochip. The detectable change can include, but is not limited to, a change in fluorescence, or a change in a physical parameter, such as electrical conductance or refractive index, at each location on the Biochip. The Biochip will then be read by a device, such as a fluorescence scanner or a surface plasmon resonance detector, that can measure the magnitude of the change at each location on the Biochip. The location of the change reveals what Target molecule has been detected, and the magnitude of the change indicates how much of it is present. The combination of these two pieces of information will yield diagnostic and prognostic medical information when signal patterns are compared with those obtained from Bodily fluids of individuals with diagnosed disorders (FIG. 1). In principle, the Biochip could be used to test any chemically complex mixture provided that Nucleic acid ligands to components suspected of being present in the mixture are attached to the Biochip. Thus, the Nucleic acid ligand Biochip will have a wider use in environmental testing, etc.
摘要翻译：披露了一种核酸配体“生物芯片”，由一种或多种特定的核酸配体以空间限定的方式连接的固体支持物组成。每个核酸配体特异性地和特异性地结合到包含在测试混合物例如体液内的特定靶分子。靶分子包括但不限于蛋白质（细胞，病毒，细菌等）激素，糖，代谢副产物，辅因子和中间体，药物和毒素。将测试混合物与Biochip接触导致Target分子与其同源核酸配体的结合。靶向核酸配体的结合在Biochip上的每个特定位置产生可检测的变化。可检测的变化可以包括但不限于在生物芯片上的每个位置的荧光的变化或物理参数的变化，例如电导率或折射率。然后，生物芯片将被诸如荧光扫描仪或表面等离子体共振检测器的装置读取，该检测器可以测量Biochip上每个位置的变化幅度。变化的位置揭示了目标分子被检测到的，变化的大小表明它有多少存在。当将信号模式与从具有诊断障碍的个体的体液获得的信号模式进行比较时，这两条信息的组合将产生诊断和预后的医学信息（图1）。原则上，生物芯片可以用于测试任何化学复合物混合物，只要核酸配体与怀疑存在于混合物中的组分连接在Biochip上即可。因此，核酸配体生物芯片将在环境测试等方面有更广泛的应用。

2. 发明授权

US06242246B1 Nucleic acid ligand diagnostic Biochip 失效
标题翻译：核酸配体诊断生物芯片
公开(公告)号：US06242246B1
公开(公告)日：2001-06-05
申请号：US08990436
申请日：1997-12-15
申请人： Larry Gold , Dan Drolet , Dom Zichi , Sumedha Jayasena , Steve Creighton , Stanley Gill
发明人： Larry Gold , Dan Drolet , Dom Zichi , Sumedha Jayasena , Steve Creighton , Stanley Gill
IPC分类号： C12M136
CPC分类号： C12Q1/6839 , C07B2200/11 , C12Q1/6834 , C12Q1/6837 , C40B40/00 , Y10S977/924 , C12Q2565/514 , C12Q2565/101 , C12Q2541/101 , C12Q2525/205
摘要： A Nucleic acid ligand “Biochip” is disclosed, consisting of a solid support to which one or more specific Nucleic acid ligands is attached in a spatially defined manner. Each Nucleic acid ligand binds specifically and avidly to a particular Target molecule contained within a Test mixture, such as a Bodily fluid The Target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. Contacting the Test mixture with the Biochip leads to the binding of a Target molecule to its cognate Nucleic acid ligand. Binding of Target to the Nucleic acid ligand results in a detectable change at each specific location on the Biochip. The detectable change can include, but is not limited to, a change in fluorescence, or a change in a physical parameter, such as electrical conductance or refractive index, at each location on the Biochip. The Biochip will then be read by a device, such as a fluorescence scanner or a surface plasma resonance detector, that can measure the magnitude of the change at each location on the Biochip. The location of the change reveals what Target molecule has been detected, and the magnitude of the change indicates how much of it is present The combination of these two pieces of information will yield diagnostic and prognostic medical information when signal patterns are compared with those obtained from Bodily fluids of individuals with diagnosed disorders FIG. 1). In principle, the Biochip could be used to test any chemically complex mixture provided that Nucleic acid ligands to components suspected of being present in the mixture are attached to the Biochip. Thus, the Nucleic acid ligand Biochip will have a wider use in environmental testing, etc.
摘要翻译：披露了一种核酸配体“生物芯片”，由一种或多种特定的核酸配体以空间限定的方式连接的固体支持物组成。每个核酸配体特异性地和特异性地结合在测试混合物（例如体液）中的特定靶分子。靶分子包括但不限于蛋白质（细胞，病毒，细菌等）激素，糖，代谢副产物，辅因子和中间体，药物和毒素。将测试混合物与Biochip接触导致Target分子与其同源核酸配体的结合。靶向核酸配体的结合在Biochip上的每个特定位置产生可检测的变化。可检测的变化可以包括但不限于在生物芯片上的每个位置的荧光的变化或物理参数的变化，例如电导率或折射率。然后，生物芯片将被诸如荧光扫描仪或表面等离子体共振检测器等器件读取，可以测量Biochip上每个位置的变化幅度。变化的位置揭示了目标分子被检测到的，变化的幅度表明它存在多少这两个信息的组合将产生诊断和预后的医学信息，当信号模式与从患有诊断障碍的个体的体液。 1）。原则上，生物芯片可以用于测试任何化学复合物混合物，只要核酸配体与怀疑存在于混合物中的组分连接在Biochip上即可。因此，核酸配体生物芯片将在环境测试等方面有更广泛的应用。

3. 发明授权

US06458543B1 Nucleic acid ligand diagnostic biochip 有权
标题翻译：核酸配体诊断生物芯片
公开(公告)号：US06458543B1
公开(公告)日：2002-10-01
申请号：US09723517
申请日：2000-11-28
申请人： Larry Gold , Dan Drolet , Dom Zichi , Sumedha Jayasena , Steve Creighton , Stanley Gill
发明人： Larry Gold , Dan Drolet , Dom Zichi , Sumedha Jayasena , Steve Creighton , Stanley Gill
IPC分类号： C12Q168
CPC分类号： C12Q1/6839 , C07B2200/11 , C12Q1/6834 , C12Q1/6837 , C40B40/00 , Y10S977/924 , C12Q2565/514 , C12Q2565/101 , C12Q2541/101 , C12Q2525/205
摘要： A Nucleic acid ligand “Biochip” is disclosed, consisting of a solid support to which one or more specific Nucleic acid ligands is attached in a spatially defined manner. Each Nucleic acid ligand binds specifically and avidly to a particular Target molecule contained within a Test mixture, such as a Bodily fluid. The Target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. In principle, the Biochip could be used to test any chemically complex mixture provided that Nucleic acid ligands to components suspected of being present in the mixture are attached to the Biochip. Thus, the Nucleic acid ligand Biochip will have a wider use in environmental testing, etc.
摘要翻译：披露了一种核酸配体“生物芯片”，由一种或多种特定的核酸配体以空间限定的方式连接的固体支持物组成。每个核酸配体特异性地和特异性地结合到包含在测试混合物例如体液内的特定靶分子。靶分子包括但不限于蛋白质（细胞，病毒，细菌等）激素，糖，代谢副产物，辅因子和中间体，药物和毒素。原则上，生物芯片可以用于测试任何化学复合物混合物，只要核酸配体与怀疑存在于混合物中的组分连接在Biochip上即可。因此，核酸配体生物芯片将在环境测试等方面有更广泛的应用。

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