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1. 发明申请

US20060019404A1 Quantitative assay with extended dynamic range 审中-公开
标题翻译：具有扩展动态范围的定量测定
公开(公告)号：US20060019404A1
公开(公告)日：2006-01-26
申请号：US11061096
申请日：2005-02-18
申请人： Joel Blatt , Michael Allen , Diem Le , Paul Patel , Patrick Sexton
发明人： Joel Blatt , Michael Allen , Diem Le , Paul Patel , Patrick Sexton
IPC分类号： G01N21/77
CPC分类号： G01N21/8483 , G01N21/78 , G01N33/689
摘要： An efficient design for an expanded dynamic range in a lateral flow one step assay for the detection of an analyte in a biological sample is disclosed. The device comprises a multiple strip design, each constructed of four zones; a sample receiving zone, a sample treatment zone, a labeling zone, and a capture zone. The sample containing analyte is accepted in the sample receiving zone in the form of blood, serum, plasma, or urine. It is then carried into the sample treatment zone where it is rendered compatible with the chemistries of the assay strip. The treated sample then flows into the labeling zone where it interacts with visible particles that are coupled to analyte specific binding proteins. The flow continues, carrying the labeled analyte into the capture zone where it is immobilized in specific regions with analyte specific binding proteins. Excess flow is absorbed in an absorbent zone that is in contact with the capture zone. A positive result is interpreted by detection of the visible particles in the specified regions of the capture zone.
摘要翻译：公开了用于在侧向流动中扩展的动态范围的有效设计，用于检测生物样品中的分析物的一步测定。该装置包括多个条带设计，每个由四个区域构成; 样品接收区，样品处理区，标记区和捕获区。含有分析物的样品在样品接收区域以血液，血清，血浆或尿液的形式被接受。然后将其运送到样品处理区域中，使其与测定条带的化学物质相容。然后将经处理的样品流入标记区域，在该区域与被分析物特异性结合蛋白偶联的可见颗粒相互作用。流动继续，将标记的分析物携带到捕获区中，在捕获区中将其固定在具有分析物特异性结合蛋白的特定区域中。在与捕获区接触的吸收区中吸收过量的流动。通过检测捕获区域的指定区域中的可见颗粒来解释正的结果。

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