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1. 发明申请

US20140220641A1 TRANSCRIPTION FACTORS FOR CELLULOSIC ENZYME PRODUCTION 有权
标题翻译：用于细胞生产的转录因子
公开(公告)号：US20140220641A1
公开(公告)日：2014-08-07
申请号：US14233735
申请日：2012-07-23
申请人： Chaoguang Tian , Teresa Shock , N. Louise Glass , Samuel Coradetti , James Craig
发明人： Chaoguang Tian , Teresa Shock , N. Louise Glass , Samuel Coradetti , James Craig
IPC分类号： C12P19/14 , C12P19/02 , C12N15/80
CPC分类号： C12P19/14 , C07K14/37 , C12N15/80 , C12P19/02
摘要： Provided herein are methods and compositions for increasing the production of one or more cellulases from a fungal host cell. The disclosure is based, on the surprising discovery that mis-expression of the transcriptional regulator clr-2 in a filamentous fungal cell was able to induce expression of cellulase genes under non-inducing or starvation conditions, resulting in increased secretion of cellulases from the cell. Advantageously, mis-expression of the transcription factor clr-2 in a filamentous fungal cell cultured in the absence of cellulose or cellobiose results in increased secretion of cellulases. The disclosure relates inter alia to a method of degrading cellulose-containing material, to a method of increasing the production of one or more cellulases from a fungal cell and to a method of reducing the viscosity of a pretreated biomass material, by contacting pretreated biomass material with a fungal host cell containing at least one recombinant nucleic acid encoding clr-2 or a related transcription factor.
摘要翻译：本文提供了用于增加一种或多种纤维素酶从真菌宿主细胞产生的方法和组合物。本发明基于惊人的发现，即转录调节因子clr-2在丝状真菌细胞中的错误表达能够在非诱导或饥饿条件下诱导纤维素酶基因的表达，导致纤维素酶从细胞中的分泌增加。有利的是，在不存在纤维素或纤维二糖的情况下培养的丝状真菌细胞中转录因子clr-2的错误表达导致纤维素酶的分泌增加。本公开尤其涉及将含纤维素材料降解的方法，一种增加一种或多种纤维素酶从真菌细胞产生的方法，以及降低预处理的生物质材料的粘度的方法，通过使预处理的生物质材料与含有至少一种编码clr-2或相关转录因子的重组核酸的真菌宿主细胞。

2. 发明授权

US09441255B2 Transcription factors for cellulosic enzyme production 有权
标题翻译：纤维素酶生产的转录因子
公开(公告)号：US09441255B2
公开(公告)日：2016-09-13
申请号：US14233735
申请日：2012-07-23
申请人： Chaoguang Tian , Teresa Shock , N. Louise Glass , Samuel Coradetti , James Craig
发明人： Chaoguang Tian , Teresa Shock , N. Louise Glass , Samuel Coradetti , James Craig
IPC分类号： C12P19/14 , C07K14/37 , C12N15/80 , C12P19/02
CPC分类号： C12P19/14 , C07K14/37 , C12N15/80 , C12P19/02
摘要： Provided herein are methods and compositions for increasing the production of one or more cellulases from a fungal host cell. The disclosure is based, on the surprising discovery that mis-expression of the transcriptional regulator clr-2 in a filamentous fungal cell was able to induce expression of cellulase genes under non-inducing or starvation conditions, resulting in increased secretion of cellulases from the cell. Advantageously, mis-expression of the transcription factor clr-2 in a filamentous fungal cell cultured in the absence of cellulose or cellobiose results in increased secretion of cellulases. The disclosure relates inter alia to a method of degrading cellulose-containing material, to a method of increasing the production of one or more cellulases from a fungal cell and to a method of reducing the viscosity of a pretreated biomass material, by contacting pretreated biomass material with a fungal host cell containing at least one recombinant nucleic acid encoding clr-2 or a related transcription factor.
摘要翻译：本文提供了用于增加一种或多种纤维素酶从真菌宿主细胞产生的方法和组合物。本发明基于惊人的发现，即转录调节因子clr-2在丝状真菌细胞中的错误表达能够在非诱导或饥饿条件下诱导纤维素酶基因的表达，导致纤维素酶从细胞中的分泌增加。有利的是，在不存在纤维素或纤维二糖的情况下培养的丝状真菌细胞中转录因子clr-2的错误表达导致纤维素酶的分泌增加。本公开尤其涉及将含纤维素材料降解的方法，一种增加一种或多种纤维素酶从真菌细胞产生的方法，以及降低预处理的生物质材料的粘度的方法，通过使预处理的生物质材料与含有至少一种编码clr-2或相关转录因子的重组核酸的真菌宿主细胞。

3. 发明授权

US08431360B2 Methods and compositions for improving sugar transport, mixed sugar fermentation, and production of biofuels 有权
公开(公告)号：US08431360B2
公开(公告)日：2013-04-30
申请号：US12843844
申请日：2010-07-26
申请人： N. Louise Glass , Chaoguang Tian , William T. Beeson, IV , Huimin Zhao , Jing Du , Jin Ho Choi , James H. Doudna Cate , Jonathan M. Galazka , Suk-Jin Ha , Yong-Su Jin , Soo Rin Kim , Sijin Li , Xiaomin Yang
发明人： N. Louise Glass , Chaoguang Tian , William T. Beeson, IV , Huimin Zhao , Jing Du , Jin Ho Choi , James H. Doudna Cate , Jonathan M. Galazka , Suk-Jin Ha , Yong-Su Jin , Soo Rin Kim , Sijin Li , Xiaomin Yang
IPC分类号： C12N1/00 , C12P21/04 , C12P21/06
CPC分类号： C12P21/02 , C07K14/37 , C07K14/705 , C12N15/80 , C12N15/81 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure further relates to methods of increasing transport of cellodextrin into a cell, methods of increasing growth of a cell on a medium containing cellodextrin, methods of co-fermenting cellulose-derived and hemicellulose-derived sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell, methods of increasing transport of a pentose into a cell, methods of increasing growth of a cell on a medium containing pentose sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell.

4. 发明申请

US20110020910A1 METHODS AND COMPOSITIONS FOR IMPROVING SUGAR TRANSPORT, MIXED SUGAR FERMENTATION, AND PRODUCTION OF BIOFUELS 有权
标题翻译：改善蔗糖运输，混合糖发酵和生产生物柴油的方法和组合物
公开(公告)号：US20110020910A1
公开(公告)日：2011-01-27
申请号：US12843844
申请日：2010-07-26
申请人： N.Louise C. GLASS , Chaoguang Tian , William T. Beeson, IV , Huimin Zhao , Jing Du , Jin Ho Choi , James H. Doudna Cate , Jonathan M. Galazka , Suk Jin Ha , Yong-Su Jin , Soo Rin Kim , Sijin Li , Xiaomin Yang
发明人： N.Louise C. GLASS , Chaoguang Tian , William T. Beeson, IV , Huimin Zhao , Jing Du , Jin Ho Choi , James H. Doudna Cate , Jonathan M. Galazka , Suk Jin Ha , Yong-Su Jin , Soo Rin Kim , Sijin Li , Xiaomin Yang
IPC分类号： C12N1/21 , C12N1/15 , C12N1/19
CPC分类号： C12P21/02 , C07K14/37 , C07K14/705 , C12N15/80 , C12N15/81 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure further relates to methods of increasing transport of cellodextrin into a cell, methods of increasing growth of a cell on a medium containing cellodextrin, methods of co-fermenting cellulose-derived and hemicellulose-derived sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell, methods of increasing transport of a pentose into a cell, methods of increasing growth of a cell on a medium containing pentose sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell.
摘要翻译：本公开内容涉及含有编码多肽的重组多核苷酸的宿主细胞，其中多肽将纤维葡聚糖转运入细胞。本发明进一步涉及增加纤维素递送到细胞中的方法，增加细胞在含有纤维葡聚糖的培养基上生长的方法，共发酵纤维素衍生和半纤维素衍生的糖的方法，以及制备烃或烃的方法通过提供含有编码多肽的重组多核苷酸的宿主细胞，其中所述多肽将纤维葡聚糖转运到细胞中。本公开涉及含有编码多肽的重组多核苷酸的宿主细胞，其中多肽将戊糖转运入细胞，增加戊糖转运至细胞中的方法，在含有戊糖的培养基上增加细胞生长的方法，以及通过提供含有编码多肽的重组多核苷酸的宿主细胞来制备烃或烃衍生物的方法，其中多肽将戊糖转运到细胞中。

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