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    • 2. 发明授权
    • Method for analysis metabolite difference between biological samples
    • 分析生物样品代谢物差异的方法
    • US07678579B2
    • 2010-03-16
    • US11784292
    • 2007-04-05
    • Byung Hwa JungBong Chul Chung
    • Byung Hwa JungBong Chul Chung
    • G01N30/02
    • G01N33/6848G01N30/7206G06F19/24Y10T436/255
    • Disclosed is a method of extracting all metabolites existing in a biological sample to detect a comprehensive difference between metabolites of a control group and a test group, thereby verifying significance thereof. More particularly, the invention comprises a first step of analyzing a fraction extracted with a solid phase extraction method, a fraction obtained by extracting the remnant fraction with a liquid-liquid extraction method and fractions extracted with the liquid-liquid extraction method at two pH different from each other after the hydrolysis, with a gas chromatography-mass spectrometry; a second step of converting the chromatogram result into numerical values capable of being statistically processed; and a third step of analyzing the numerical values with a principal component analysis (PCA) and a discriminant analysis (DA) to detect a difference between the control group and the test group. According to the invention, the difference between the two groups can be comprehensively detected with the areas of the peaks on the chromatogram, without a standard material or verified quantitative method. Therefore, it is possible to detect the metabolic change in the organism due to the disease or gene mutation, without an accurate quantitative analysis of the metabolites.
    • 公开了提取生物样品中存在的所有代谢物的方法,以检测对照组和试验组的代谢物之间的综合差异,从而验证其意义。 更具体地说,本发明包括分析用固相萃取法萃取的馏分的第一步骤,用液 - 液萃取方法提取剩余馏分获得的馏分,并用液 - 液萃取方法在两种pH值下提取馏分 水解后,用气相色谱 - 质谱联用; 将色谱图结果转换为能够进行统计处理的数值的第二步骤; 以及通过主成分分析(PCA)和判别式分析(DA)分析数值以检测对照组和测试组之间的差异的第三步骤。 根据本发明,可以用色谱图上峰的面积全面检测两组之间的差异,无需标准材料或经过验证的定量方法。 因此,可以检测由于疾病或基因突变而导致的生物体的代谢变化,而不对该代谢产物进行准确的定量分析。
    • 3. 发明申请
    • CLOCK-BASED DATA STORAGE DEVICE, DUAL PULSE GENERATION DEVICE, AND DATA STORAGE DEVICE
    • 基于时钟的数据存储设备,双脉冲发生器件和数据存储器件
    • US20090185437A1
    • 2009-07-23
    • US12034556
    • 2008-02-20
    • Bai Sun KongSung Chan KangByung Hwa Jung
    • Bai Sun KongSung Chan KangByung Hwa Jung
    • G11C7/00G11C8/00H03K3/00
    • G11C11/412H03K3/012H03K3/356121
    • Disclosed is a clock-based data storage device, which includes a dual pulse generating device and a data starge device having two dynamic nodes for prior chargement/dischargement. The clock-based data storage device includes a dual pulse generating unit which delays a clock signal and then outputs a first clock signal corresponding to inversion of a clock signal and a second clock signal corresponding to the clock signal by using the delayed clock signal when the clock signal shifts, a pull-up wait for outputting a pull-up output signal to an output port, based on the first clock signal outputted from the dual pulse generating unit and an input data signal which has beeb inputted, a pull-down unit for outputting a pull-down output signal to the output port, based on the second clock signal outputted from the dual pulse generating unit and the input data signal inputted which has been inputted, and a latch unit which is disposed between the pull-up and pull-down units, and the output port so as to store at least one output signal outputted f roars the pull-down unit as well as the pull-down unit.
    • 公开了一种基于时钟的数据存储装置,其包括双脉冲发生装置和具有两个动态节点用于事先充电/放电的数据扩充装置。 基于时钟的数据存储装置包括双脉冲发生单元,其延迟时钟信号,然后通过使用延迟的时钟信号输出对应于时钟信号的反相的第一时钟信号和对应于时钟信号的第二时钟信号 基于从双脉冲发生单元输出的第一时钟信号和输入了贝贝的输入数据信号,上拉等待输出端口的上拉输出信号的上拉等待时间信号移位,下拉单元 用于基于从双脉冲发生单元输出的第二时钟信号和输入的输入数据信号向输出端口输出下拉输出信号;以及锁存单元,其设置在上拉和下拉 下拉单元和输出端口,以便存储输出的至少一个输出信号和下拉单元以及下拉单元。
    • 4. 发明授权
    • Clock-based data storage device, dual pulse generation device, and data storage device
    • 基于时钟的数据存储设备,双脉冲发生设备和数据存储设备
    • US07724605B2
    • 2010-05-25
    • US12034556
    • 2008-02-20
    • Bai Sun KongSung Chan KangByung Hwa Jung
    • Bai Sun KongSung Chan KangByung Hwa Jung
    • G11C8/00
    • G11C11/412H03K3/012H03K3/356121
    • Disclosed is a clock-based data storage device, which includes a dual pulse generating device and a data starge device having two dynamic nodes for prior chargement/dischargement. The clock-based data storage device includes a dual pulse generating unit which delays a clock signal and then outputs a first clock signal corresponding to inversion of a clock signal and a second clock signal corresponding to the clock signal by using the delayed clock signal when the clock signal shifts, a pull-up wait for outputting a pull-up output signal to an output port, based on the first clock signal outputted from the dual pulse generating unit and an input data signal which has beeb inputted, a pull-down unit for outputting a pull-down output signal to the output port, based on the second clock signal outputted from the dual pulse generating unit and the input data signal inputted which has been inputted, and a latch unit which is disposed between the pull-up and pull-down units, and the output port so as to store at least one output signal outputted from the pull-down unit as well as the pull-down unit.
    • 公开了一种基于时钟的数据存储装置,其包括双脉冲发生装置和具有两个动态节点用于事先充电/放电的数据扩充装置。 基于时钟的数据存储装置包括双脉冲发生单元,其延迟时钟信号,然后通过使用延迟的时钟信号输出对应于时钟信号的反相的第一时钟信号和对应于时钟信号的第二时钟信号 基于从双脉冲发生单元输出的第一时钟信号和输入信号输入的输入数据信号,下拉单元,时钟信号移位,上拉等待输出端口输出上拉输出信号; 用于基于从双脉冲发生单元输出的第二时钟信号和输入的输入数据信号向输出端口输出下拉输出信号;以及锁存单元,其设置在上拉和下拉 下拉单元和输出端口,以便存储从下拉单元输出的至少一个输出信号以及下拉单元。
    • 6. 发明申请
    • Method for analysis of metabolite difference between two biological samples using gas chromatography-mass spectrometry
    • 使用气相色谱 - 质谱法分析两种生物样品之间代谢物差异的方法
    • US20080076181A1
    • 2008-03-27
    • US11784292
    • 2007-04-05
    • Byung Hwa JungBong Chul Chung
    • Byung Hwa JungBong Chul Chung
    • G01N30/02G01N1/28
    • G01N33/6848G01N30/7206G06F19/24Y10T436/255
    • Disclosed is a method of extracting all metabolites existing in a biological sample to detect a comprehensive difference between metabolites of a control group and a test group, thereby verifying significance thereof. More particularly, the invention comprises a first step of analyzing a fraction extracted with a solid phase extraction method, a fraction obtained by extracting the remnant fraction with a liquid-liquid extraction method and fractions extracted with the liquid-liquid extraction method at two pH different from each other after the hydrolysis, with a gas chromatography-mass spectrometry; a second step of converting the chromatogram result into numerical values capable of being statistically processed; and a third step of analyzing the numerical values with a principal component analysis (PCA) and a discriminant analysis (DA) to detect a difference between the control group and the test group. According to the invention, the difference between the two groups can be comprehensively detected with the areas of the peaks on the chromatogram, without a standard material or verified quantitative method. Therefore, it is possible to detect the metabolic change in the organism due to the disease or gene mutation, without an accurate quantitative analysis of the metabolites.
    • 公开了提取生物样品中存在的所有代谢物的方法,以检测对照组和试验组的代谢物之间的综合差异,从而验证其意义。 更具体地说,本发明包括分析用固相萃取法萃取的馏分的第一步骤,用液 - 液萃取方法提取剩余馏分获得的馏分,并用液 - 液萃取方法在两种pH值下提取馏分 水解后,用气相色谱 - 质谱联用; 将色谱图结果转换为能够进行统计处理的数值的第二步骤; 以及通过主成分分析(PCA)和判别式分析(DA)分析数值以检测对照组和测试组之间的差异的第三步骤。 根据本发明,可以用色谱图上峰的面积全面检测两组之间的差异,无需标准材料或经过验证的定量方法。 因此,可以检测由于疾病或基因突变而导致的生物体的代谢变化,而不对该代谢产物进行准确的定量分析。