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1. 发明申请

US20110217718A1 Methods and Kits for Distinguishing Between Specific and Non-Specific Protein Associations 有权
标题翻译：用于区分特异性和非特异性蛋白质结合的方法和试剂盒
公开(公告)号：US20110217718A1
公开(公告)日：2011-09-08
申请号：US13106490
申请日：2011-05-12
申请人： Brian T. Chait , Alan J. Tackett , Jeffrey A. DeGrasse , Marlene Oeffinger , Michael P. Rout , Matthew D. Sekedat
发明人： Brian T. Chait , Alan J. Tackett , Jeffrey A. DeGrasse , Marlene Oeffinger , Michael P. Rout , Matthew D. Sekedat
IPC分类号： G01N33/68
CPC分类号： G01N33/5023 , G01N33/5032 , G01N33/60 , G01N33/6845 , G01N33/6848
摘要： The present invention is method of determining whether or not associations between a given protein and other proteins in a cell are specific. The method comprises (a) providing a first sample of the cells in which the given protein contains a tag, (b) providing a second sample of the same cells, wherein the given protein and the other proteins are metabolically labeled, and wherein neither the given protein nor the other proteins are tagged, (c) mixing and lysing the first cell sample and the second cell sample to provide a mixture of proteins, (d) binding the tag of the given protein to a substrate, (e) isolating proteins associated with the tagged given protein bound to the substrate, whereby the associated proteins comprise: (i) proteins specifically associated with the tagged given protein, (ii) proteins non-specifically associated with the tagged given protein, or (iii) a combination thereof, (f) determining whether each associated protein is unlabeled or a mixture of labeled and unlabeled proteins, wherein if the associated protein is not labeled, then that protein was specifically associated in the cell with the tagged given protein.
摘要翻译：本发明是确定细胞中给定蛋白质和其它蛋白质之间的缔合是否是特异性的方法。该方法包括（a）提供其中给定蛋白质含有标签的细胞的第一样品，（b）提供相同细胞的第二样品，其中所述给定蛋白质和其它蛋白质被代谢标记，并且其中（c）混合和裂解第一细胞样品和第二细胞样品以提供蛋白质的混合物，（d）将给定蛋白质的标签与底物结合，（e）分离蛋白质与标记的给定蛋白质结合至底物，其中相关蛋白质包含：（i）与标记的给定蛋白质特异性相关的蛋白质，（ii）与标记的给定蛋白质非特异性相关的蛋白质，或（iii）其组合，（f）确定每个相关蛋白是否未标记，或标记蛋白和未标记蛋白质的混合物，其中如果相关蛋白未被标记，那么该蛋白质与标记特异性结合在细胞中给定蛋白质。

2. 发明授权

US06446010B1 Method for assessing significance of protein identification 失效
标题翻译：评估蛋白质鉴定意义的方法
公开(公告)号：US06446010B1
公开(公告)日：2002-09-03
申请号：US09333726
申请日：1999-06-15
申请人： Jan Eriksson , David Feny{haeck over (o)} , Brian T. Chait
发明人： Jan Eriksson , David Feny{haeck over (o)} , Brian T. Chait
IPC分类号： G06F1900
CPC分类号： G01N33/6848 , C07K1/00 , C07K1/047 , G06F19/18
摘要： A method of generating a frequency distribution of scores comprising: a) generating mass data for a biological molecule; b) generating mass data for a series of random hypothetical biological molecules; c) calculating a frequency distribution of high similarity scores between mass data of each molecule generated in steps a and b.
摘要翻译：一种产生分数的频率分布的方法，包括：a）产生生物分子的质量数据; b）产生一系列随机假设生物分子的质量数据; c）计算在步骤a和b中产生的每个分子的质量数据之间的高相似性分数的频率分布。

3. 发明授权

US06271037B1 Method and product for the sequence determination of peptides using a mass spectrometer 失效
标题翻译：使用质谱仪对肽进行序列测定的方法和产品
公开(公告)号：US06271037B1
公开(公告)日：2001-08-07
申请号：US08341555
申请日：1996-06-24
申请人： Brian T. Chait , Ronald Beavis , Rong Wang , Stephen B. H. Kent
发明人： Brian T. Chait , Ronald Beavis , Rong Wang , Stephen B. H. Kent
IPC分类号： G01N3300
CPC分类号： G01N33/6842 , C07K1/107 , C07K1/128 , G01N33/6824 , Y10T436/24 , Y10T436/25 , Y10T436/255
摘要： Method is described for sequencing polypeptides by forming peptide ladders comprising a series of polypeptides in which adjacent members of the series vary by one amino acid residue and determining the identity and position of each amino acid in the polypeptide by mass spectroscopy.
摘要翻译：描述了通过形成包含一系列多肽的肽梯级来测序多肽的方法，其中该序列的相邻成员变化一个氨基酸残基，并通过质谱确定多肽中每个氨基酸的同一性和位置。

4. 发明授权

US5643798A Instrument and method for the sequencing of genome 失效
标题翻译：用于测序基因组的仪器和方法
公开(公告)号：US5643798A
公开(公告)日：1997-07-01
申请号：US473230
申请日：1995-06-07
申请人： Ronald C. Beavis , Brian T. Chait
发明人： Ronald C. Beavis , Brian T. Chait
IPC分类号： B01J19/00 , C12Q1/68 , C40B40/06 , C40B60/14 , H01J49/04 , H01J49/16 , G01N33/00 , C12N15/00
CPC分类号： H01J49/0413 , B01J19/0046 , C12Q1/6872 , H01J49/164 , B01J2219/00364 , B01J2219/00536 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00628 , B01J2219/00644 , B01J2219/00659 , B01J2219/00689 , B01J2219/00722 , C40B40/06 , C40B60/14 , Y10S436/807 , Y10T436/143333 , Y10T436/24
摘要： Improved techniques are provided for DNA sequencing, and particularly for sequencing of the entire human genome. Different base-specific reactions are utilized to use different sets of DNA fragments from a piece of DNA of unknown sequence. Each of the different sets of DNA fragments has a common origin and terminates at a particular base along the unknown sequence. The molecular weight of the DNA fragments in each of the different sets is detected by a matrix assisted laser absorption mass spectrometer to determine the sequence of the different bases in the DNA. The methods and apparatus of the present invention provide a relatively simple and low cost technique which may be automated to sequence thousands of gene bases per hour, and eliminates the tedious and time consuming gel electrophoresis separation technique conventionally used to determine the masses of DNA fragments.
摘要翻译：提供改进的技术用于DNA测序，特别是用于整个人类基因组的测序。使用不同的碱基特异性反应来使用来自未知序列的DNA片段的不同组DNA片段。不同组DNA片段中的每一个具有共同起源，并且沿未知序列终止于特定基因。通过基质辅助激光吸收质谱仪检测每个不同组中的DNA片段的分子量，以确定DNA中不同碱基的序列。本发明的方法和装置提供了一种相对简单和低成本的技术，其可以自动化以每小时序列数千个基因碱基，并消除常规用于确定DNA片段质量的繁琐且耗时的凝胶电泳分离技术。

5. 发明授权

US08227198B2 Methods and kits for distinguishing between specific and non-specific protein associations 有权
标题翻译：用于区分特异性和非特异性蛋白质关联的方法和试剂盒
公开(公告)号：US08227198B2
公开(公告)日：2012-07-24
申请号：US13106490
申请日：2011-05-12
申请人： Brian T. Chait , Alan J. Tackett , Jeffrey A. DeGrasse , Marlene Oeffinger , Michael P. Rout , Matthew D. Sekedat
发明人： Brian T. Chait , Alan J. Tackett , Jeffrey A. DeGrasse , Marlene Oeffinger , Michael P. Rout , Matthew D. Sekedat
IPC分类号： G01N33/53
CPC分类号： G01N33/5023 , G01N33/5032 , G01N33/60 , G01N33/6845 , G01N33/6848
摘要： The present invention is method of determining whether or not associations between a given protein and other proteins in a cell are specific. The method comprises (a) providing a first sample of the cells in which the given protein contains a tag, (b) providing a second sample of the same cells, wherein the given protein and the other proteins are metabolically labeled, and wherein neither the given protein nor the other proteins are tagged, (c) mixing and lysing the first cell sample and the second cell sample to provide a mixture of proteins, (d) binding the tag of the given protein to a substrate, (e) isolating proteins associated with the tagged given protein bound to the substrate, whereby the associated proteins comprise: (i) proteins specifically associated with the tagged given protein, (ii) proteins non-specifically associated with the tagged given protein, or (iii) a combination thereof, (f) determining whether each associated protein is unlabeled or a mixture of labeled and unlabeled proteins, wherein if the associated protein is not labeled, then that protein was specifically associated in the cell with the tagged given protein.
摘要翻译：本发明是确定细胞中给定蛋白质和其它蛋白质之间的缔合是否是特异性的方法。该方法包括（a）提供其中给定蛋白质含有标签的细胞的第一样品，（b）提供相同细胞的第二样品，其中所述给定蛋白质和其它蛋白质代谢标记，并且其中（c）混合和裂解第一细胞样品和第二细胞样品以提供蛋白质的混合物，（d）将给定蛋白质的标签与底物结合，（e）分离蛋白质与标记的给定蛋白质结合至底物，其中相关蛋白质包含：（i）与标记的给定蛋白质特异性相关的蛋白质，（ii）与标记的给定蛋白质非特异性相关的蛋白质，或（iii）其组合，（f）确定每个相关蛋白是否未标记，或标记蛋白和未标记蛋白质的混合物，其中如果相关蛋白未被标记，那么该蛋白质与标记特异性结合在细胞中给定蛋白质。

6. 发明授权

US06642059B2 Method for the comparative quantitative analysis of proteins and other biological material by isotopic labeling and mass spectroscopy 有权
公开(公告)号：US06642059B2
公开(公告)日：2003-11-04
申请号：US09949510
申请日：2001-09-06
申请人： Brian T. Chait , David Cowburn , Yoshi Oda
发明人： Brian T. Chait , David Cowburn , Yoshi Oda
IPC分类号： G01N2400
CPC分类号： G01N33/6842 , G01N33/6803 , Y10T436/24 , Y10T436/25 , Y10T436/25375
摘要： The present invention is a method for accurately comparing the levels of cellular components, such as proteins, present in samples which differ in some respect from each other using mass spectroscopy and isotopic labeling. A first sample of biological matter, such as cells, is cultured in a first medium and a second sample of the same biological matter is cultured in a second medium, wherein at least one isotope in the second medium has a different abundance than the abundance of the same isotope in the first medium. One of the samples is modulated, such as by treatment with a bacteria, a virus, a drug, hormone, a chemical or an environmental stimulus. The samples are combined and at least one protein is removed. The removed protein is subjected to mass spectroscopy to develop a mass spectrum. A ratio is computed between the peak intensities of at least one closely spaced pair of peaks to determine the relative abundance of the protein in each sample. The protein is identified by the mass spectrum or through other techniques known in the art. Modifications to the proteins, such as the phosphorylation of the protein, and the site of the modification may also be determined through the process of the present invention. The method is applicable to the components of any type of biological matter which are ionizable and may therefore be analyzed by mass spectroscopy.

7. 发明授权

US5792664A Methods for producing and analyzing biopolymer ladders 失效
标题翻译：生物聚合物梯子的生产和分析方法
公开(公告)号：US5792664A
公开(公告)日：1998-08-11
申请号：US474997
申请日：1995-06-07
申请人： Brian T. Chait , Rong Wang , Stephen B. H. Kent , Steven M. Clark
发明人： Brian T. Chait , Rong Wang , Stephen B. H. Kent , Steven M. Clark
IPC分类号： G01N33/68 , A61K38/06 , C07H21/00 , C07K1/12 , C12P21/06 , C12Q1/37 , C12Q1/68 , G01N33/00 , G03C1/52
CPC分类号： C07H21/00 , C07K1/12 , C12P21/06 , C12Q1/37 , G01N33/6818 , Y10T436/24
摘要： Methods of producing biopolymer ladders and their use to obtain structural information about the biopolymer. The ladders are produced by setting up catalytic cleavage and terminating reactions at the end of biopolymer molecules. The terminating reactions terminate cleavage of a percentage of the biopolymer molecules at each round of cleavage.
摘要翻译：生产生物聚合物梯子的方法及其获得生物聚合物结构信息的用途。梯子通过在生物聚合物分子的末端设置催化裂解和终止反应而产生。终止反应终止了在每轮切割时百分比的生物聚合物分子的裂解。

8. 发明授权

US5245186A Electrospray ion source for mass spectrometry 失效
标题翻译：电喷雾离子源进行质谱分析
公开(公告)号：US5245186A
公开(公告)日：1993-09-14
申请号：US879035
申请日：1992-05-06
申请人： Brian T. Chait , Swapan K. Chowdhury , Viswanatham Katta , Urooj A. Mirza
发明人： Brian T. Chait , Swapan K. Chowdhury , Viswanatham Katta , Urooj A. Mirza
IPC分类号： H01J49/04
CPC分类号： H01J49/165
摘要： A mass analyzer for the analysis of mass spectra of ions derived from organic molecules includes an electrospray ion source having means to transport the molecules of interest in a solvent of pure water free of organic solvents. The electrospray ion source sprays the water solution, under an imposed voltage, through a metal syringe needle having a sharp etched point and into a conductive capillary tube having a sharpened entrance orifice.
摘要翻译：用于分析源自有机分子的离子的质谱的质量分析器包括电喷雾离子源，其具有将目标分子输送到不含有机溶剂的纯水溶剂中的方法。电喷雾离子源通过具有尖锐蚀刻点的金属注射器针头在施加的电压下喷射水溶液并进入具有尖锐入口的导电毛细管中。

9. 发明授权

US06824981B2 Ultra-sensitive detection systems using alterable peptide tags 失效
标题翻译：使用可变肽标签的超灵敏检测系统
公开(公告)号：US06824981B2
公开(公告)日：2004-11-30
申请号：US09929266
申请日：2001-08-13
申请人： Brian T. Chait , Darin R. Latimer , Paul M. Lizardi , Eric R. Kershnar , Jon S. Morrow , Matthew E. Roth , Martin J. Mattessich , Kevin J. McConnell
发明人： Brian T. Chait , Darin R. Latimer , Paul M. Lizardi , Eric R. Kershnar , Jon S. Morrow , Matthew E. Roth , Martin J. Mattessich , Kevin J. McConnell
IPC分类号： C12Q168
CPC分类号： G01N33/6842 , C07K1/047 , C07K1/1077 , C07K1/13 , C07K7/06 , C07K7/08 , G01N33/6803
摘要： Disclosed are compositions and methods for sensitive detection of one or multiple analytes. In general, the methods involve the use of special label components, referred to as reporter signals, that can be associated with, incorporated into, or otherwise linked to the analytes. In some embodiments, the reporter signals can be altered such that the altered forms of different reporter signals can be distinguished from each other. In some embodiments, sets of reporter signals can be used where two or more of the reporter signals in a set have one or more common properties that allow the reporter signals having the common property to be distinguished and/or separated from other molecules lacking the common property. In other embodiments, sets of reporter signal/analyte conjugates can be used where two or more of the reporter signal/analyte conjugates in a set have one or more common properties that allow the reporter signal/analyte conjugates having the common property to be distinguished and/or separated form other molecules lacking the common property. Reporter signals can also be in conjunction with analytes (such as in mixtures of reporter signals and analytes), where no significant physical association between the reporter signals and analytes occurs; or alone, where no analyte is present.
摘要翻译：公开了用于敏感检测一种或多种分析物的组合物和方法。通常，这些方法涉及使用称为报告信号的特殊标记成分，其可以与分析物相关联，并入或以其它方式连接到分析物上。在一些实施方案中，可以改变报道信号，使得不同报道信号的改变形式可以彼此区分。在一些实施方案中，可以使用一组报告信号，其中一组中的两个或更多个报告基因信号具有一个或多个共同性质，其允许具有共同性质的报道信号被区分和/或与其他分子不相同属性。在其它实施方案中，可以使用组的报告信号/分析物缀合物，其中组中的两个或多个报道信号/分析物缀合物具有一个或多个共同性质，其允许区分具有共同性质的报道信号/分析物缀合物， /或分离形成其他缺乏共同性质的分子。记者信号也可以与分析物（例如报告信号和分析物的混合物）结合，其中报告信号和分析物之间不存在明显的物理结合; 或单独存在，其中不存在分析物。

10. 发明授权

US06750061B2 Method and product for the sequence determination of peptides using a mass spectrometer 失效
标题翻译：使用质谱仪对肽进行序列测定的方法和产品
公开(公告)号：US06750061B2
公开(公告)日：2004-06-15
申请号：US09828326
申请日：2001-04-05
申请人： Brian T. Chait , Ronald Beavis , Rong Wang , Stephen B. H. Kent
发明人： Brian T. Chait , Ronald Beavis , Rong Wang , Stephen B. H. Kent
IPC分类号： G01N3300
CPC分类号： G01N33/6842 , C07K1/107 , C07K1/128 , G01N33/6824 , Y10T436/24 , Y10T436/25 , Y10T436/255
摘要： Method is described for sequencing polypeptides by forming peptide ladders comprising a series of polypeptides in which adjacent members of the series vary by one amino acid residue and determining the identity and position of each amino acid in the polypeptide by mass spectroscopy.
摘要翻译：描述了通过形成包含一系列多肽的肽梯级来测序多肽的方法，其中该序列的相邻成员变化一个氨基酸残基，并通过质谱确定多肽中每个氨基酸的同一性和位置。

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