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1. 发明申请

US20080261282A1 Fermentation Process for Preparing Coenzyme Q10 by the Recombinant Agrobacterium tumefaciens 审中-公开
标题翻译：由重组农杆菌制备辅酶Q10的发酵方法
公开(公告)号：US20080261282A1
公开(公告)日：2008-10-23
申请号：US12044379
申请日：2008-03-07
申请人： Soo-Ryun Cheong , Sang-Young Kim , Jung-Kul Lee , Hyeon-Cheol Lee , Suk-Jin Ha , Bong-Seoung Koo , Ji-Hyun Yoo
发明人： Soo-Ryun Cheong , Sang-Young Kim , Jung-Kul Lee , Hyeon-Cheol Lee , Suk-Jin Ha , Bong-Seoung Koo , Ji-Hyun Yoo
IPC分类号： C12P7/66
CPC分类号： C12N9/1022 , C12P7/66
摘要： The present invention relates to a transformed Agrobacterium tumefaciens BNQ-pGPRX11 (Accession No. KCCM-10554) harboring a recombinant expression vector (pGPRX11). Further, the present invention also provides a fermentation method for maximum production of coenzyme Q10 using a transformed Agrobacterium tumefaciens deposited to Korean Culture Center of Microorganism with accession number KCCM-10554 comprising the steps of: i) constructing the recombinant expression vector pGPRX11 containing decaprenyl diphosphate synthase gene and 1-deoxy-D-xylulose 5-phosphate synthase gene (SEQ ID NO: 1); ii) preparing a transformed Agrobacterium tumefaciens (KCCM-10554) by harboring said recombinant expression vector pGPRX11 to the host of Agrobacterium tumefaciens BNQ 0605 (KCCM-10413); iii) growing the transformed cells on growth medium comprising 50 g/L of sucrose, 15 g/L of yeast extract, 15 g/L of peptone and 7.5 g/L of NaCl; iv) fermenting transformed cells on production medium comprising 30˜50 g/L of corn steep powder, 0.3˜0.7 g/L of KH2PO4, 0.3˜0.7 g/L of K2HPO4, 12˜18 g/L of ammonium sulfate, 1.5˜2.5 g/L of lactic acid, 0.2˜0.3 g/L of magnesium sulfate on condition that aeration rate of the medium is 0.8˜1.2 volume of air per volume of medium per minute, temperature is 30˜34°C. and pH is 6.0˜8.0; v) removing the transformed cells and other residue from the fermentation medium; and vi) separating and recovering coenzyme Q10 from the fermentation medium of step (v).
摘要翻译：本发明涉及带有重组表达载体（pGPRX11）的转化的根癌农杆菌BNQ-pGPRX11（登录号KCCM-10554）。此外，本发明还提供了使用保藏于韩国文化中心微生物的转化的根癌土壤杆菌，使用登录号KCCM-10554最大限度地生产辅酶Q 10的发酵方法，包括以下步骤：i）构建含有十聚丙烯酰二磷酸合成酶基因和1-脱氧-D-木酮糖-5-磷酸合酶基因（SEQ ID NO：1）的重组表达载体pGPRX11; ii）通过将所述重组表达载体pGPRX11携带到根癌农杆菌BNQ 0605（KCCM-10413）的宿主中来制备转化的根癌土壤杆菌（KCCM-10554）; iii）在包含50g / L蔗糖，15g / L酵母提取物，15g / L蛋白胨和7.5g / L NaCl的生长培养基上培养转化细胞; iv）在包含30〜50g / L的玉米糠粉末，0.3〜0.7g / L的KH 2 PO 4，0.3〜0.7g的生产培养基中发酵转化的细胞 / L的HPO 4，12〜18g / L的硫酸铵，1.5〜2.5g / L的乳酸，0.2〜0.3g / L的镁硫酸盐的条件是介质的通气速率为每分钟体积介质的0.8〜1.2体积空气，温度为30〜34℃。 pH 6.0〜8.0; v）从发酵培养基中去除转化的细胞和其它残留物; 和vi）从步骤（v）的发酵培养基中分离并回收辅酶Q 10。

2. 发明申请

US20060134757A1 Microorganism and the process for the production of teicoplanin 失效
标题翻译：微生物和生产替考拉宁的过程
公开(公告)号：US20060134757A1
公开(公告)日：2006-06-22
申请号：US11013736
申请日：2004-12-17
申请人： Soo-Ryun Cheong , Sang-Young Kim , Youn-Woo Lee , Jung-Kul Lee , Hyeon-Cheol Lee , Hyung-Moo Jung , Bong-Seoung Koo
发明人： Soo-Ryun Cheong , Sang-Young Kim , Youn-Woo Lee , Jung-Kul Lee , Hyeon-Cheol Lee , Hyung-Moo Jung , Bong-Seoung Koo
IPC分类号： C12P19/28 , C12N1/20
CPC分类号： C12R1/045 , C12P19/60
摘要： The present invention provides a newly isolated mutant culture of Actinoplanes teichomyceticus BNG 2315. This culture is capable of producing teicoplanin more than 60 times productivity than those reported before (e.g., Actinoplanes teichomyceticus nov. sp. ATCC 31121). The present invention also provides a fermentation process for the production of teicoplanin in an aerobic condition using the mutant strain Actinoplanes teichomyceticus BNG 2315 in a culture-medium comprising carbon sources, nitrogen sources and mineral salts.
摘要翻译：本发明提供了一种新近分离的灭活细胞巨噬细胞BNG2315的突变培养物。该培养物能够产生超过60倍生产力的泰可菌素（例如，Actinoplanes teichomyceticus nov。sp。ATCC 31121）。本发明还提供了在含有碳源，氮源和矿物盐的培养基中，使用突变体细胞壁虫TNG 2315在需氧条件下生产替考拉宁的发酵方法。

3. 发明授权

US07432080B2 Process for the production of teicoplanin 失效
标题翻译：替考拉宁生产工艺
公开(公告)号：US07432080B2
公开(公告)日：2008-10-07
申请号：US11013736
申请日：2004-12-17
申请人： Soo-Ryun Cheong , Sang-Young Kim , Youn-Woo Lee , Jung-Kul Lee , Hyeon-Cheol Lee , Hyung-Moo Jung , Bong-Seoung Koo
发明人： Soo-Ryun Cheong , Sang-Young Kim , Youn-Woo Lee , Jung-Kul Lee , Hyeon-Cheol Lee , Hyung-Moo Jung , Bong-Seoung Koo
IPC分类号： C12P21/04 , C12P21/00
CPC分类号： C12R1/045 , C12P19/60
摘要： The present invention provides a newly isolated mutant culture of Actinoplanes teichomyceticus BNG 2315. This culture is capable of producing teicoplanin more than 60 times productivity than those reported before (e.g., Actinoplanes teichomyceticus nov. sp. ATCC 31121). The present invention also provides a fermentation process for the production of teicoplanin in an aerobic condition using the mutant strain Actinoplanes teichomyceticus BNG 2315 in a culture-medium comprising carbon sources, nitrogen sources and mineral salts.
摘要翻译：本发明提供了一种新近分离的灭活细胞巨噬细胞BNG2315的突变培养物。该培养物能够产生超过60倍生产力的泰可菌素（例如，Actinoplanes teichomyceticus nov。sp。ATCC 31121）。本发明还提供了在含有碳源，氮源和矿物盐的培养基中，使用突变体细胞壁虫TNG 2315在需氧条件下生产替考拉宁的发酵方法。

4. 发明申请

US20050181490A1 Fermentation process for preparing coenzyme Q10 by the recombinant Agrobacterium tumefaciens 审中-公开
标题翻译：由重组农杆菌制备辅酶Q10的发酵方法
公开(公告)号：US20050181490A1
公开(公告)日：2005-08-18
申请号：US11042209
申请日：2005-01-26
申请人： Soo-Ryun Cheong , Sang-Young Kim , Jung-Kul Lee , Hyeon-Cheol Lee , Suk-Jin Ha , Bong-Seoung Koo , Ji-Hyun Yoo
发明人： Soo-Ryun Cheong , Sang-Young Kim , Jung-Kul Lee , Hyeon-Cheol Lee , Suk-Jin Ha , Bong-Seoung Koo , Ji-Hyun Yoo
IPC分类号： C12N15/09 , C12N1/21 , C12N9/10 , C12N9/12 , C12N9/88 , C12N15/74 , C12P7/66 , C12Q1/68 , C12R1/01
CPC分类号： C12P7/66 , C12N9/1022
摘要： The present invention relates to a transformed Agrabacterium tumefaciens BNQ-pGPRX11 (Accession No. KCCM-10554) harboring a recombinant expression vector (pGPRX11). Further, the present invention also provides a fermentation method for maximum production of coenzyme Q10 using a transformed Agrabacterium tumefaciens deposited to Korean Culture Center of Microorganism with accession number KCCM-10554 comprising the steps of: i) fermenting transformed cells on production medium comprising 30˜50 g/L of corn steep powder, 0.3˜0.7 g/L of KH2PO4, 0.3˜0.7 g/L of K2HPO4, 12˜18 g/L of ammonium sulfate, 1.5˜2.5 g/L of lactic acid, 0.2˜0.3 g/L of magnesium sulfate on condition that aeration rate of the medium is 0.8˜1.2 volume of air per volume of medium per minute, temperature 30˜34° C. and pH is 6.0˜8.0; ii) removing the transformed cells and other residue from the fermentation medium; and iii) separating and recovering coenzyme Q10 from the fermentation medium of step (ii).
摘要翻译：本发明涉及携带重组表达载体（pGPRX11）的转化的根癌农杆菌BNQ-pGPRX11（登录号KCCM-10554）。此外，本发明还提供了使用保藏于韩国文化中心的保藏号为KCCM-10554的转化的根癌农杆菌来最大限度地生产辅酶Q 10的发酵方法，包括以下步骤：i）发酵包含30〜50g / L的玉米糠粉，0.3〜0.7g / L的KH 2 PO 3，0.3〜0.7g / L的生产培养基中的转化细胞， K 2 N 2 HPO 4，12〜18g / L硫酸铵，1.5〜2.5g / L乳酸，0.2〜0.3g / L硫酸镁条件培养基的通气速率为每分钟体积培养基的空气量为0.8〜1.2，温度为30〜34℃，pH为6.0〜8.0; ii）从发酵培养基中去除转化的细胞和其它残留物; 和iii）从步骤（ii）的发酵培养基中分离并回收辅酶Q 10。

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