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1. 发明授权

US08952217B2 Process for decreasing verbascose in a plant by expression of a chloroplast-targeted fimD protein 有权
标题翻译：通过表达叶绿体靶向的fimD蛋白在植物中减少毛蕊花的方法
公开(公告)号：US08952217B2
公开(公告)日：2015-02-10
申请号：US11516230
申请日：2006-09-06
申请人： Piotr Puzio , Birgit Wendel , Michael Manfred Herold , Ralf Looser , Astrid Blau , Gunnar Plesch , Beate Kamlage , Florian Schauwecker
发明人： Piotr Puzio , Birgit Wendel , Michael Manfred Herold , Ralf Looser , Astrid Blau , Gunnar Plesch , Beate Kamlage , Florian Schauwecker
IPC分类号： C12N15/82 , C07H21/04 , A01H5/00 , C12P7/64 , C12P13/06 , C12P13/10 , C12P13/12
CPC分类号： C12N15/8214 , C12N15/8221 , C12N15/8243 , C12N15/8245 , C12N15/8247 , C12N15/825 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12P7/64 , C12P13/06 , C12P13/10 , C12P13/12
摘要： The present invention relates to a process for decreasing verbascose in a plant by expression of a chloroplast-targeted polypeptide which is a member of the fimD superfamily in a plant cell, plant, or a part thereof. The invention furthermore relates to a process for producing a plant cell, plant, or part thereof with a decrease in the amount of verbascose. Also provided are nucleic acid constructs and vectors useful for practicing the methods as well as plants, plant tissues, propagation materials and harvested materials thus obtained. Agricultural compositions comprising the plant materials thus obtained is also provided.
摘要翻译：本发明涉及通过在植物细胞，植物或其一部分中表达作为fimD超家族的成员的叶绿体靶向多肽来减少植物中的毛蕊花的方法。本发明还涉及一种用于生产植物细胞，植物或其部分的方法，其具有减少量的量。还提供了用于实施方法以及由此获得的植物，植物组织，繁殖材料和收获的材料的核酸构建体和载体。还提供了包含如此获得的植物材料的农业组合物。

2. 发明申请

US20140199313A1 Process for the Production of Fine Chemicals 审中-公开
标题翻译：精细化工生产工艺
公开(公告)号：US20140199313A1
公开(公告)日：2014-07-17
申请号：US11885489
申请日：2006-02-28
申请人： Gunnar Plesch , Piotr Puzio , Astrid Blau , Ralf Looser , Birgit Wendel , Beate Kamlage , Micheal Manfred Herold , Florian Schauwecker
发明人： Gunnar Plesch , Piotr Puzio , Astrid Blau , Ralf Looser , Birgit Wendel , Beate Kamlage , Micheal Manfred Herold , Florian Schauwecker
IPC分类号： A61K39/395 , C12N15/63 , C12N5/10 , C12N1/00 , C12N15/74 , C07K16/16 , A01H5/00 , A61K31/7088 , A61K38/16 , A01H1/00 , C07H21/00 , C07K14/415
CPC分类号： C12N15/8214 , C12N15/8221 , C12N15/8243 , C12N15/8245 , C12N15/8247 , C12N15/825 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12P7/64 , C12P13/06 , C12P13/10 , C12P13/12
摘要： The present invention relates to a process for the production of the fine chemical in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof, preferably in plastids. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.
摘要翻译：本发明涉及在微生物，植物细胞，植物，植物组织或其一个或多个部分，优选在质体中生产精细化学品的方法。本发明还涉及核酸分子，多肽，核酸构建体，载体，抗体，宿主细胞，植物组织，繁殖材料，收获的材料，植物，微生物以及农业组合物及其用途。

3. 发明申请

US20090300794A1 Manipulation Of The Nitrogen Metabolism 审中-公开
标题翻译：操纵氮代谢
公开(公告)号：US20090300794A1
公开(公告)日：2009-12-03
申请号：US12302421
申请日：2007-05-23
申请人： Gunnar Plesch , Piotr Puzio , Astrid Blau , Ralf Looser , Birgit Wendel , Beate Kamlage , Oliver Schmitz
发明人： Gunnar Plesch , Piotr Puzio , Astrid Blau , Ralf Looser , Birgit Wendel , Beate Kamlage , Oliver Schmitz
IPC分类号： C12N15/82 , C12N5/04 , C12N15/29 , C12N5/10 , C12P21/02 , C07K14/415 , C07K16/16 , A01H5/00 , C12N1/21 , C12Q1/68 , A01H5/10
CPC分类号： C12N15/8243 , C07K14/245 , C07K14/395 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to the manipulation of the nitrogen metabolism in photosynthetic active organisms, preferably in plants. In particular, the present invention relates to a process for the enhanced nitrogen assimilation, accumulation and/or utilization and/or for the increased total nitrogen content in a photosynthetic active organism.
摘要翻译：本发明涉及光合活性生物体中优选在植物中氮代谢的操作。特别地，本发明涉及增强氮同化，积累和/或利用和/或增加光合活性生物体中总氮含量的方法。

4. 发明申请

US20060117401A1 Method for producing aminoacids 审中-公开
标题翻译：生产氨基酸的方法
公开(公告)号：US20060117401A1
公开(公告)日：2006-06-01
申请号：US10539954
申请日：2003-12-19
申请人： Oliver Schmitz , Piotr Puzio , Astrid Blau , Ralf Looser , Birgit Wendel , Beate Kamlage , Gunnar Plesch
发明人： Oliver Schmitz , Piotr Puzio , Astrid Blau , Ralf Looser , Birgit Wendel , Beate Kamlage , Gunnar Plesch
IPC分类号： A01H1/00 , C07H21/04 , C12P13/04 , C12N15/87 , C12N1/21 , C12N1/18 , C12N15/82 , C12N15/74
CPC分类号： C12P13/12 , C12N9/1014 , C12N9/88 , C12N15/8251 , C12N15/8253
摘要： The invention relates to a method for producing aminoacids in transgenic organisms. The inventive method consists of the following steps: a) introduction of nucleic acids sequence which codes threonine decomposing protein or lysine decomposing protein or codes threonine decomposing protein and lysine decomposing protein, b) introduction of nucleic acids sequence which improves the decomposition of threonine or lysine or the decomposition of threonine and lysine in the transgenic organisms; c) expression of (a) or (b) nucleic acids sequence in a transgenic organism. In a very useful manner, the nucleic acids sequence is introduced in the step a) of the method, said sequence being selected from: i) the nucleic acids sequence with the sequence present in SEQ ID NO: 1, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23 and/or SEQ ID NO:25; ii) the nucleic acids sequence which is preserved as a result of a degenerate genetic code by re-recording aminoacids sequence present in SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24 and/or 26; and iii) a derivative of the nucleic add sequence present in SEQ ID NO: 1, SEQ ID NO:11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23 and/or SEQ ID NO:25 which codes polypeptides with the nucleic acids sequence present in SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24 and/or 26 and which comprises at least 50% of homology in terms of aminoacids without reducing the biological activity of polypeptides.
摘要翻译：本发明涉及一种在转基因生物中产生氨基酸的方法。本发明方法包括以下步骤：a）引入编码苏氨酸分解蛋白或赖氨酸分解蛋白或编码苏氨酸分解蛋白和赖氨酸分解蛋白的核酸序列，b）引入改善苏氨酸或赖氨酸分解的核酸序列或转基因生物中苏氨酸和赖氨酸的分解; c）转基因生物中（a）或（b）核酸序列的表达。以非常有用的方式，在方法的步骤a）中引入核酸序列，所述序列选自：i）具有SEQ ID NO：1，SEQ ID NO：11中存在的序列的核酸序列， SEQ ID NO：13，SEQ ID NO：15，SEQ ID NO：17，SEQ ID NO：19，SEQ ID NO：21，SEQ ID NO：23和/或SEQ ID NO：25; ii）通过重新记录SEQ ID NO：2，SEQ ID NO：12，SEQ ID NO：14，SEQ ID NO：16，SEQ ID NO：16所示的氨基酸序列，作为简并遗传密码的结果而保存的核酸序列 SEQ ID NO：18，SEQ ID NO：20，SEQ ID NO：22，SEQ ID NO：24和/或26; 和iii）存在于SEQ ID NO：1，SEQ ID NO：11，SEQ ID NO：13，SEQ ID NO：15，SEQ ID NO：17，SEQ ID NO：19，SEQ ID NO：19中的核酸添加序列的衍生物编码多肽与SEQ ID NO：2，SEQ ID NO：12，SEQ ID NO：14，SEQ ID NO：16中存在的核酸序列的SEQ ID NO：21，SEQ ID NO：23和/或SEQ ID NO：25 ，SEQ ID NO：18，SEQ ID NO：20，SEQ ID NO：22，SEQ ID NO：24和/或26，并且其在氨基酸方面包含至少50％的同源性而不降低多肽的生物学活性。

5. 发明申请

US20120005777A1 Process for the Production of Fine Chemicals 审中-公开
标题翻译：精细化工生产工艺
公开(公告)号：US20120005777A1
公开(公告)日：2012-01-05
申请号：US13180964
申请日：2011-07-12
申请人： Gunnar Plesch , Piotr Puzio , Astrid Blau , Ralf Looser , Birgit Wendel , Beate Kamlage , Agnes Chardonnens , Amber Shirley , Xi-Qing Wang , Rodrigo Sarria-Millan , Bryan McKersie , Ruoying Chen
发明人： Gunnar Plesch , Piotr Puzio , Astrid Blau , Ralf Looser , Birgit Wendel , Beate Kamlage , Agnes Chardonnens , Amber Shirley , Xi-Qing Wang , Rodrigo Sarria-Millan , Bryan McKersie , Ruoying Chen
IPC分类号： A01H1/00 , A23K1/00 , A23L1/212 , A23L1/48 , C12Q1/68 , C12Q1/02 , C12P21/06 , C12N5/10 , C12N1/00 , C12N1/21 , C12N1/15 , C12N1/19 , C12N15/63 , A61K38/00 , A61K31/7088 , C07H21/04 , A01H5/00 , C12N15/87 , A61K39/395
CPC分类号： C12P13/001 , C12N15/8242 , C12P13/04
摘要： The present invention relates to a process for the production of fine chemicals in a microorganism, a plant cell, a plant, a plant tissue or parts thereof. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagtion material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.
摘要翻译：本发明涉及在微生物，植物细胞，植物，植物组织或其部分中生产精细化学品的方法。本发明还涉及核酸分子，多肽，核酸构建体，载体，反义分子，抗体，宿主细胞，植物组织，繁殖材料，收获的材料，植物，微生物以及农业组合物及其用途。

6. 发明申请

US20070118916A1 Process for the production of fine chemicals 有权
标题翻译：生产精细化学品的工艺
公开(公告)号：US20070118916A1
公开(公告)日：2007-05-24
申请号：US11516230
申请日：2006-09-06
申请人： Piotr Puzio , Birgit Wendel , Michael Herold , Ralf Looser , Astrid Blau , Gunnar Plesch , Beate Kamlage , Florian Schauwecker
发明人： Piotr Puzio , Birgit Wendel , Michael Herold , Ralf Looser , Astrid Blau , Gunnar Plesch , Beate Kamlage , Florian Schauwecker
IPC分类号： A01H1/00 , C07H21/04 , C12N9/10 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8214 , C12N15/8221 , C12N15/8243 , C12N15/8245 , C12N15/8247 , C12N15/825 , C12N15/8251 , C12N15/8253 , C12N15/8254 , C12P7/64 , C12P13/06 , C12P13/10 , C12P13/12
摘要： The present invention relates to a process for the production of the fine chemical in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof, preferably in plastids. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.
摘要翻译：本发明涉及在微生物，植物细胞，植物，植物组织或其一个或多个部分，优选在质体中生产精细化学品的方法。本发明还涉及核酸分子，多肽，核酸构建体，载体，抗体，宿主细胞，植物组织，繁殖材料，收获的材料，植物，微生物以及农业组合物及其用途。

7. 发明授权

US06538120B1 Genomic sequence of the human &mgr;-opioid receptor gene and the variants, polymorphisms and mutations thereof 失效
标题翻译：人类μ-阿片受体基因的基因组序列及其变体，多态性和突变
公开(公告)号：US06538120B1
公开(公告)日：2003-03-25
申请号：US09355709
申请日：1999-09-27
申请人： Margret Hoehe , Birgit Wendel
发明人： Margret Hoehe , Birgit Wendel
IPC分类号： C07H2102
CPC分类号： C07K14/705 , A61K38/00
摘要： The invention concerns the genomic sequence of the human &mgr; opioid receptor gene and its variants, polymorphisms and mutations.
摘要翻译：本发明涉及人类μ阿片受体基因及其变体，多态性和突变的基因组序列。

8. 发明申请

US20130340119A1 Method for Increasing Yield and Fine Chemical Production in Plants 审中-公开
标题翻译：增加植物产量和精细化学品生产的方法
公开(公告)号：US20130340119A1
公开(公告)日：2013-12-19
申请号：US13883450
申请日：2011-10-27
申请人： Gunnar Plesch , Astrid Blau , Michael Manfred Herold , Beate Kamlage , Birgit Wendel , Piotr Puzio , Oliver Bläsing , Oliver Thimm , Janneke Hendriks , Christophe Reuzeau
发明人： Gunnar Plesch , Astrid Blau , Michael Manfred Herold , Beate Kamlage , Birgit Wendel , Piotr Puzio , Oliver Bläsing , Oliver Thimm , Janneke Hendriks , Christophe Reuzeau
IPC分类号： C12N15/82
CPC分类号： C12N15/8247 , A23D9/02 , C07K14/415 , C11B1/10 , C11C3/00 , C11C3/003 , C12N15/8245
摘要： A method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a POI (Protein Of Interest) polypeptide is provided. Methods for the production of plants having modulated expression of a nucleic acid encoding a DnaJ-like chaperone polypeptide are provided, in which plants have enhanced yield-related traits compared to control plants. Nucleic acids encoding DnaJ-like chaperone, constructs comprising the same and uses thereof are also provided.
摘要翻译：提供了一种通过调节植物中编码POI（Protein Of Interest）多肽的核酸表达来增强植物中产量相关性状的方法。提供了具有调节表达编码DnaJ样分子伴侣多肽的核酸的植物的方法，其中与对照植物相比，植物具有增强的产量相关性状。还提供了编码DnaJ样分子伴侣的核酸，包含其的构建体及其用途。

9. 发明申请

US20110035841A1 Lipid Metabolism Proteins, Combinations of Lipid Metabolism Proteins and Uses Thereof 审中-公开
标题翻译：脂质代谢蛋白，脂质代谢蛋白的组合及其用途
公开(公告)号：US20110035841A1
公开(公告)日：2011-02-10
申请号：US12808703
申请日：2008-12-10
申请人： Gunnar Plesch , Piotr Puzio , Astrid Blau , Michael Manfred Herold , Birgit Wendel , Beate Kamlage , Florian Schauwecker , Thorsten Zank , Oliver Oswald , Tom Wetjen
发明人： Gunnar Plesch , Piotr Puzio , Astrid Blau , Michael Manfred Herold , Birgit Wendel , Beate Kamlage , Florian Schauwecker , Thorsten Zank , Oliver Oswald , Tom Wetjen
IPC分类号： C12N15/82 , C07H21/02 , C07H21/04 , C12N15/63 , C12N5/10 , C12P21/02 , C07K14/25 , C07K14/245 , C07K16/12 , A01H5/00
CPC分类号： C12N15/8247 , C12N15/8242 , C12N15/8261 , Y02A40/146
摘要： Described herein are inventions in the field of genetic engineering of plants, including combinations of polynucleotides encoding LMPs to improve agronomic, horticultural, and quality traits. This invention also relates to the combination of polynucleotides encoding proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to LMP polynucleotides encoding lipid metabolism proteins (LMP) and the use of these combinations of these sequences, their order and direction in the combination, and the regulatory elements used to control expression and transcript termination in these combinations in transgenic plants. In particular, the invention is directed to methods for manipulating fatty acid-related compounds and for increasing oil and starch levels and altering the fatty acid composition in plants and seeds. The invention further relates to methods of using these novel combinations of polypeptides to stimulate plant growth, and/or root growth and/or to increase yield and/or composition of seed storage compounds.
摘要翻译：本文描述了植物遗传工程领域的发明，包括编码LMP的多核苷酸的组合，以改善农艺学，园艺学和品质性状。本发明还涉及编码与种子储存化合物在植物中的存在有关的蛋白质的多核苷酸的组合。更具体地，本发明涉及编码脂质代谢蛋白（LMP）的LMP多核苷酸以及这些序列的这些组合的用途，它们在组合中的顺序和方向，以及用于控制这些组合中的表达和转录终止的调控元件转基因植物。特别地，本发明涉及用于操作脂肪酸相关化合物和增加油和淀粉水平并改变植物和种子中的脂肪酸组成的方法。本发明还涉及使用多肽的这些新型组合来刺激植物生长和/或根生长和/或增加种子储存化合物的产量和/或组成的方法。

10. 发明申请

US20050155100A1 Population of transgenic plants, biological material derived therefrom, corresponding plasmid collection and population of transformed host organisms, use thereof and methods for producing the same 有权
标题翻译：转基因植物的种群，衍生自其的生物材料，相应的质粒收集和转化的宿主生物群体，其用途及其制备方法
公开(公告)号：US20050155100A1
公开(公告)日：2005-07-14
申请号：US10508264
申请日：2003-03-18
申请人： Astrid Blau , Mathieu Klein , Birgit Wendel
发明人： Astrid Blau , Mathieu Klein , Birgit Wendel
IPC分类号： C12N1/21 , C12N9/04 , C12N9/12 , C12P7/10 , A01H1/00 , C12N15/82
CPC分类号： C12N9/0006 , C12N9/1205 , C12P7/10 , Y02E50/16
摘要： The present invention relates to populations of trangenic plants encompassing a substantial part of all codogenic gene segments of a donor organism, and to biological material derived therefrom, plasmid collections and populations of transformed host organisms with which plants can be transformed in a suitable manner. There are also described methods for generating the plants and the material, and the use of the plants and of the material for functional studies. The codogenic gene segments are integrated into the genome of the plants. For example, there are described a population of plants of the species Arabidopsis thaliana into whose genome the codogenic gene segments from Saccharomyces cerevisiae are integrated, and their morphological analysis under normal conditions and stress conditions.
摘要翻译：本发明涉及包含供体生物体的所有编码基因区段的大部分的转基因植物的群体，以及从其衍生的生物材料，质粒收集物和转化的宿主生物群体，以适当的方式转化植物。还描述了用于产生植物和材料的方法，以及植物和材料用于功能研究的用途。编码基因片段整合到植物的基因组中。例如，描述了拟南芥拟南芥的植物种群，其基因组中含有酿酒酵母的编码基因片段，以及它们在正常条件和胁迫条件下的形态学分析。

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