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    • 4. 发明申请
    • Method for producing aminoacids
    • 生产氨基酸的方法
    • US20060117401A1
    • 2006-06-01
    • US10539954
    • 2003-12-19
    • Oliver SchmitzPiotr PuzioAstrid BlauRalf LooserBirgit WendelBeate KamlageGunnar Plesch
    • Oliver SchmitzPiotr PuzioAstrid BlauRalf LooserBirgit WendelBeate KamlageGunnar Plesch
    • A01H1/00C07H21/04C12P13/04C12N15/87C12N1/21C12N1/18C12N15/82C12N15/74
    • C12P13/12C12N9/1014C12N9/88C12N15/8251C12N15/8253
    • The invention relates to a method for producing aminoacids in transgenic organisms. The inventive method consists of the following steps: a) introduction of nucleic acids sequence which codes threonine decomposing protein or lysine decomposing protein or codes threonine decomposing protein and lysine decomposing protein, b) introduction of nucleic acids sequence which improves the decomposition of threonine or lysine or the decomposition of threonine and lysine in the transgenic organisms; c) expression of (a) or (b) nucleic acids sequence in a transgenic organism. In a very useful manner, the nucleic acids sequence is introduced in the step a) of the method, said sequence being selected from: i) the nucleic acids sequence with the sequence present in SEQ ID NO: 1, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23 and/or SEQ ID NO:25; ii) the nucleic acids sequence which is preserved as a result of a degenerate genetic code by re-recording aminoacids sequence present in SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24 and/or 26; and iii) a derivative of the nucleic add sequence present in SEQ ID NO: 1, SEQ ID NO:11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23 and/or SEQ ID NO:25 which codes polypeptides with the nucleic acids sequence present in SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24 and/or 26 and which comprises at least 50% of homology in terms of aminoacids without reducing the biological activity of polypeptides.
    • 本发明涉及一种在转基因生物中产生氨基酸的方法。 本发明方法包括以下步骤:a)引入编码苏氨酸分解蛋白或赖氨酸分解蛋白或编码苏氨酸分解蛋白和赖氨酸分解蛋白的核酸序列,b)引入改善苏氨酸或赖氨酸分解的核酸序列 或转基因生物中苏氨酸和赖氨酸的分解; c)转基因生物中(a)或(b)核酸序列的表达。 以非常有用的方式,在方法的步骤a)中引入核酸序列,所述序列选自:i)具有SEQ ID NO:1,SEQ ID NO:11中存在的序列的核酸序列, SEQ ID NO:13,SEQ ID NO:15,SEQ ID NO:17,SEQ ID NO:19,SEQ ID NO:21,SEQ ID NO:23和/或SEQ ID NO:25; ii)通过重新记录SEQ ID NO:2,SEQ ID NO:12,SEQ ID NO:14,SEQ ID NO:16,SEQ ID NO:16所示的氨基酸序列,作为简并遗传密码的结果而保存的核酸序列 SEQ ID NO:18,SEQ ID NO:20,SEQ ID NO:22,SEQ ID NO:24和/或26; 和iii)存在于SEQ ID NO:1,SEQ ID NO:11,SEQ ID NO:13,SEQ ID NO:15,SEQ ID NO:17,SEQ ID NO:19,SEQ ID NO:19中的核酸添加序列的衍生物 编码多肽与SEQ ID NO:2,SEQ ID NO:12,SEQ ID NO:14,SEQ ID NO:16中存在的核酸序列的SEQ ID NO:21,SEQ ID NO:23和/或SEQ ID NO:25 ,SEQ ID NO:18,SEQ ID NO:20,SEQ ID NO:22,SEQ ID NO:24和/或26,并且其在氨基酸方面包含至少50%的同源性而不降低多肽的生物学活性。