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1. 发明申请

US20140011960A1 CELL-ADHESIVE PHOTOCONTROLLABLE BASE MATERIAL 审中-公开
标题翻译：细胞粘合光电材料基材
公开(公告)号：US20140011960A1
公开(公告)日：2014-01-09
申请号：US14002192
申请日：2012-02-06
申请人： Tomohiro Konno , Kazuhiko Ishihara , Batzaya Byambaa , Hisashi Sugiyama , Satoshi Ozawa
发明人： Tomohiro Konno , Kazuhiko Ishihara , Batzaya Byambaa , Hisashi Sugiyama , Satoshi Ozawa
IPC分类号： C12N5/071
CPC分类号： C12N5/0602 , C12M25/02 , C12M33/00 , C12M47/04
摘要： In analyzing, fractionating, and culturing cells alive, operations can be more simply made in real time and culture can be performed while removing unnecessary cells from cultured cells for purification. Desired cells are also analyzed and fractionated from the cultured cells to increase the purity, recovery rate, and viability of the cells. A cell-adhesive photocontrollable base material is used in which light irradiation causes the bond dissociation of a photolabile group comprising an O-nitrobenzyl skeleton to irreversibly change the surface of the irradiated portion thereof from that of the cell-adhesive material to that of a non-cell-adhesive material. Cell images are detected and analyzed to obtain the positional information of desired cells. Based on this information, areas among cells and a cell-adhesive photocontrollable material are cut by second light irradiation. On the other hand, the surface of the base material is changed from a cell-adhesive one to a non-cell-adhesive one by first light irradiation to produce the detachment between cells and the base material. This enables cells to be analyzed and fractionated alive.
摘要翻译：在细胞分析，分离和培养细胞中，可以更简单地进行操作，并且可以在从培养细胞中除去不需要的细胞以进行纯化的同时进行培养。还对所需的细胞进行分析并从培养的细胞中分离，以提高细胞的纯度，回收率和活力。使用细胞粘合剂可光控制的基材，其中光照射导致包含O-硝基苄基骨架的光不稳定基团的键解离不可逆地将其照射部分的表面从细胞粘合剂材料的表面改变为非 - - 细胞粘合剂材料。检测并分析细胞图像以获得所需细胞的位置信息。基于该信息，通过第二次光照射来切断细胞和细胞粘合剂可光控制材料中的区域。另一方面，通过第一光照射将基材的表面从电池粘合剂的表面改变为非电池粘合的表面，以产生电池与基材之间的分离。这使得细胞能够被分析和活化分离。

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