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1. 发明授权

US09879229B2 Method of viral production in cells 有权
公开(公告)号：US09879229B2
公开(公告)日：2018-01-30
申请号：US14004501
申请日：2012-02-15
申请人： Chun Fang Shen , Amine Kamen
发明人： Chun Fang Shen , Amine Kamen
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , C12N2500/00 , C12N2500/02 , C12N2710/10051 , C12N2710/14051 , C12N2720/12051
摘要： A method of using a cell to produce virus is provided involving growing cells under hyperosmotic conditions during a growth phase of the cells, infecting or transfecting the cells grown under hyperosmotic conditions with a virus, and maintaining the infected or transfected cells under less stressful conditions during a production phase of the infected or transfected cells to produce more of the virus. Viral productivity is improved by this method.

2. 发明申请

US20080113336A1 Detection Of Intact Recombinant Viruses 有权
标题翻译：检测完整重组病毒
公开(公告)号：US20080113336A1
公开(公告)日：2008-05-15
申请号：US11661932
申请日：2005-11-18
申请人： Julia Transfiguracion , Amine Kamen
发明人： Julia Transfiguracion , Amine Kamen
IPC分类号： C12Q1/70
CPC分类号： C12Q1/68 , C12Q1/70 , G01N30/02 , G01N2030/8809 , G01N2030/8813 , C12Q2565/137 , C12Q2563/173 , C12Q2545/114 , C12Q2527/15 , C12Q2523/32 , B01D15/34 , B01D15/361 , B01D15/36
摘要： In accordance with the present invention, there is provided a new method for detection and quantification of virus and viral particles, comprising the step of labeling the nucleic acids of an intact virus or viral particle with a dye that emits fluorescence once complexed with the nucleic acids and detecting the virus or viral particle with a chromatograph equipped with a fluorescence detector.
摘要翻译：根据本发明，提供了一种用于病毒和病毒颗粒的检测和定量的新方法，其包括用与核酸复合的荧光发射的染料标记完整病毒或病毒颗粒的核酸的步骤并用配有荧光检测器的色谱仪检测病毒或病毒颗粒。

3. 发明申请

US20050250177A1 Preparation, characterization and quantification of active fractions that promote cell growth and recombinant-protein expression 审中-公开
$Preparation, characterization and quantification of active fractions that promote cell growth and recombinant-protein expression$
标题翻译：促进细胞生长和重组蛋白表达的活性级分的制备，表征和定量
公开(公告)号：US20050250177A1
公开(公告)日：2005-11-10
申请号：US10637017
申请日：2003-08-07
申请人： Faustinus Yeboah , Barbara Jardin , Yasuo Konishi , Amine Kamen , Tin Wong , Soo Lee
发明人： Faustinus Yeboah , Barbara Jardin , Yasuo Konishi , Amine Kamen , Tin Wong , Soo Lee
IPC分类号： C12N5/00 , C12N5/02 , C12N5/06 , C12P21/06
CPC分类号： C12N5/0018 , C12N2500/70 , C12N2500/74 , C12N2500/92
摘要： A process for preparing an active animal cell-growth-enhancing fraction of a hydrolysate of plant tissue, animal tissue or microorganism ultrafiltrate material, especially a fraction of a yeastolate ultrafiltrate, comprising forming a precipitate from said fraction with a water-soluble solvent is disclosed. Such solvents as alkanols, alkyl sulfoxides, ketones or alkyl nitriles, especially lower (C1-C5) alkanols, particularly ethanol, can be used. The invention also relates to an active animal cell-growth-enhancing fraction of a hydrolysate of plant tissue, animal tissue or microorganism ultrafiltrate material, especially a fraction of a yeastolate ultrafiltrate, substantially free of aromatic and methyl group bearing compounds which has improved cell-growth-enhancing properties.
摘要翻译：公开了一种用于制备植物组织，动物组织或微生物超滤液物质的水解产物的活性动物细胞生长增强部分的方法，特别是酵母醇酯超级滤液的一部分，包括用水溶性溶剂从所述级分形成沉淀物。可以使用诸如链烷醇，烷基亚砜，酮或烷基腈，特别是较低（C 1 -C 5 -C 5）烷醇，特别是乙醇的溶剂。本发明还涉及植物组织，动物组织或微生物超滤液物质的水解产物的活性动物细胞生长增强级分，特别是基本上不含芳香族和甲基基团化合物的酵母醇酯超级滤液的一部分，生长增强性能。

4. 发明申请

US20050170450A1 Enhanced production of recombinant proteins by transient transfection of suspension-growing mammalian cells 审中-公开
标题翻译：通过瞬时转染悬浮培养的哺乳动物细胞增强重组蛋白的产生
公开(公告)号：US20050170450A1
公开(公告)日：2005-08-04
申请号：US10477148
申请日：2002-05-07
申请人： Yves Durocher , Sylvie Perret , Phuong Pham , Amine Kamen
发明人： Yves Durocher , Sylvie Perret , Phuong Pham , Amine Kamen
IPC分类号： C12N5/071 , C12N15/85 , C12P21/02 , C12P21/06 , C12N5/08
摘要： Disclosed is a new process for the production of recombinant proteins, by transient transfection of suspension-grown human embryonic kidney cells (293 cell line and its genetic variants) with an expression vector, using polyethylenimine (PEI) as a transfection reagent. In a preferred embodiment, the process uses 293E cells expressing the Epstein-Barr virus (EBV) EBNA 1 protein, in combination with an oriP-based episomal expression vector having an improved cytomegalovirus expression cassette comprising the CMV5 promoter. The process combines in a single step the cell growth, transfection and protein expression, is carried out without changing the culture medium, and allows to achieve high expression levels in a short period of time. The process may be carried out in a serum-free, low-protein culture medium, is easily scalable, compatible with continuous production processes, and fully adapted to high-throughput production of milligram quantities of recombinant proteins.
摘要翻译：公开了通过使用聚乙烯亚胺（PEI）作为转染试剂用表达载体瞬时转染悬浮培养的人胚胎肾细胞（293细胞系及其遗传变体）来生产重组蛋白质的新方法。在优选的实施方案中，该方法使用表达爱泼斯坦 - 巴尔病毒（EBV）EBNA 1蛋白的293E细胞与具有包含CMV5启动子的改良的巨细胞病毒表达盒的基于oriP的附加型表达载体的组合。该过程在一个步骤中结合细胞生长，转染和蛋白质表达，不改变培养基进行，并允许在短时间内达到高表达水平。该方法可以在无血清的低蛋白培养基中进行，易于扩展，与连续生产过程相容，并且完全适应于高产量的毫克量的重组蛋白质。

5. 发明授权

US07754421B2 Detection of intact recombinant viruses 有权
标题翻译：检测完整重组病毒
公开(公告)号：US07754421B2
公开(公告)日：2010-07-13
申请号：US11661932
申请日：2005-11-18
申请人： Julia Transfiguracion , Amine Kamen
发明人： Julia Transfiguracion , Amine Kamen
IPC分类号： C12Q1/70 , C12Q1/68 , G01N30/96 , C12N7/02
CPC分类号： C12Q1/68 , C12Q1/70 , G01N30/02 , G01N2030/8809 , G01N2030/8813 , C12Q2565/137 , C12Q2563/173 , C12Q2545/114 , C12Q2527/15 , C12Q2523/32 , B01D15/34 , B01D15/361 , B01D15/36
摘要： In accordance with the present invention, there is provided a new method for detection and quantification of virus and viral particles, comprising the step of labeling the nucleic acids of an intact virus or viral particle with a dye that emits fluorescence once complexed with the nucleic acids and detecting the virus or viral particle with a chromatograph equipped with a fluorescence detector.
摘要翻译：根据本发明，提供了一种用于病毒和病毒颗粒的检测和定量的新方法，其包括用与核酸复合的荧光发射的染料标记完整病毒或病毒颗粒的核酸的步骤并用配有荧光检测器的色谱仪检测病毒或病毒颗粒。

6. 发明申请

US20140004568A1 Method of Viral Production in Cells 有权
标题翻译：细胞中病毒生产方法
公开(公告)号：US20140004568A1
公开(公告)日：2014-01-02
申请号：US14004501
申请日：2012-02-15
申请人： Chun Fang Shen , Amine Kamen
发明人： Chun Fang Shen , Amine Kamen
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , C12N2500/00 , C12N2500/02 , C12N2710/10051 , C12N2710/14051 , C12N2720/12051
摘要： A method of using a cell to produce virus is provided involving growing cells under hyperosmotic conditions during growth phase of the cells, infecting or transfecting the cells grown under hyperosmotic conditions with a virus, and maintaining the infected or transfected cells under less stressful conditions during a production phase of the infected or transfected cells to produce more of the virus. Viral productivity is improved by this method.
摘要翻译：提供了使用细胞产生病毒的方法，包括在高渗渗条件下在细胞生长期生长细胞，感染或转染在高渗渗条件下用病毒生长的细胞，以及在感染或转染的细胞在感染或转染细胞的生产阶段产生更多的病毒。通过这种方法改善病毒生产力。

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