专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20140004514A1 Snap-Back Primers And Detectable Hairpin Structures 有权
标题翻译： Snap-Back引物和可检测的发夹结构
公开(公告)号：US20140004514A1
公开(公告)日：2014-01-02
申请号：US13899230
申请日：2013-05-21
申请人： Third Wave Technologies, Inc.
发明人： Jeff G. Hall , Andrew A. Lukowiak , Patrick Peterson
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6813 , C12Q1/6827 , C12Q2525/301 , C12Q2561/109
摘要： The present invention provides methods, compositions, and kits comprising snap-back primers used for forming 3′ hairpin structures, 5′ hairpin structures, and double hairpin structures. The hairpin structures may be used for detecting target sequences (e.g., such as small RNA target sequence), for detecting polymorphisms in target sequences (e.g., such as polymorphisms located near the 5′ or 3′ ends of the target sequence), or other nucleic acid characterization methods. In certain embodiments, the hairpin structures form invasive cleavage structures (e.g., in combination with a probe or upstream oligonucleotide) which may be cleaved by structure-specific enzymes in order to detect the presence or absence of a particular nucleotide or nucleotide sequence.
摘要翻译：本发明提供了包含用于形成3'发夹结构，5'发夹结构和双发夹结构的快速反应引物的方法，组合物和试剂盒。发夹结构可用于检测目标序列（例如，例如小RNA靶序列），用于检测靶序列中的多态性（例如，位于靶序列的5'或3'末端附近的多态性）或其他核酸表征方法。在某些实施方案中，发夹结构形成侵入性切割结构（例如，与探针或上游寡核苷酸组合），其可被结构特异性酶切割以检测特定核苷酸或核苷酸序列的存在或不存在。

2. 发明授权

US08071750B2 Determination of hepatitis C virus genotype 有权
标题翻译：丙型肝炎病毒基因型的测定
公开(公告)号：US08071750B2
公开(公告)日：2011-12-06
申请号：US12346322
申请日：2008-12-30
申请人： Vecheslav A. Elagin , Scott M. Law , Bjork Hill
发明人： Vecheslav A. Elagin , Scott M. Law , Bjork Hill
IPC分类号： C07H21/04
CPC分类号： C12Q1/6883 , C12Q2600/156
摘要： The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.
摘要翻译：本发明提供用于检测和表征HCV序列的组合物和方法。更具体地，本发明提供了用于使用侵入性切割结构测定（例如INVADER测定）来筛选例如来自患者的核酸样品以确定HCV基因型的组合物，方法和试剂盒。

3. 发明授权

US07824862B2 Methods for analyzing nucleic acid 有权
标题翻译：分析核酸的方法
公开(公告)号：US07824862B2
公开(公告)日：2010-11-02
申请号：US12242508
申请日：2008-09-30
申请人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
发明人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6832 , C12Q1/701 , C12Q2565/133 , C12Q2521/301
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译：本发明涉及用于分析核酸的方法和组合物。特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。

4. 发明授权

US07700750B2 Connexin allele detection assays 失效
标题翻译：连接蛋白等位基因检测试验
公开(公告)号：US07700750B2
公开(公告)日：2010-04-20
申请号：US10754408
申请日：2004-01-09
申请人： Andrea L. Mast , Erin Dorn , Robert W. Kwiatkowski, Jr. , Molly Accola , Susan S. Wigdal
发明人： Andrea L. Mast , Erin Dorn , Robert W. Kwiatkowski, Jr. , Molly Accola , Susan S. Wigdal
IPC分类号： C07H21/02 , C07H21/04 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6883 , C07H21/04 , C12Q2600/156
摘要： The present invention provides compositions and methods for the detection and characterization of mutations associated with non-syndromic hearing impairment. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of mutations in the Connexin 26, or gap junction beta 2, gene associated with non-syndromic hearing loss.
摘要翻译：本发明提供了用于检测和表征与非综合征性听力损伤相关的突变的组合物和方法。更具体地，本发明提供了组合物，方法和试剂盒，用于使用侵入性切割结构测定法（例如INVADER测定法）筛选核酸样品，例如来自患者，用于在连接蛋白26中存在突变集合中的任何一个，或间隙连接β2，与非综合征性听力损失相关的基因。

5. 发明申请

US20090203018A1 NUCLEIC ACID DETECTION ASSAYS EMPLOYING BLOCKER OLIGONUCLEOTIDES 有权
标题翻译：使用阻塞剂寡核苷酸的核酸检测测定
公开(公告)号：US20090203018A1
公开(公告)日：2009-08-13
申请号：US12360819
申请日：2009-01-27
申请人： Poonam Agarwal , Robert W. Kwiatkowski, JR.
发明人： Poonam Agarwal , Robert W. Kwiatkowski, JR.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6823 , C12Q2527/107 , C12Q2537/163 , C12Q2561/109
摘要： The present invention provides methods, compositions, and kits for detecting the presence or absence of target sequences in a sample, where the sample also contains interfering sequences that are similar or identical to the target sequences. In particular, the present invention provides blocker oligonucleotides that at least partially inhibit the formation of invasive cleavage structures with the interfering sequences but do not substantially inhibit the formation of invasive cleavage structures with the target sequences.
摘要翻译：本发明提供用于检测样品中靶序列存在或不存在的方法，组合物和试剂盒，其中样品还含有与靶序列相似或相同的干扰序列。特别地，本发明提供了阻断型寡核苷酸，该阻断剂寡核苷酸至少部分地抑制了具有干扰序列形成侵入性切割结构，但基本上不抑制靶序列形成侵入性切割结构。

6. 发明授权

US07541145B2 Cleavage of nucleic acids 失效
标题翻译：切断核酸
公开(公告)号：US07541145B2
公开(公告)日：2009-06-02
申请号：US11183078
申请日：2005-07-15
申请人： James R. Prudent , Jeff G. Hall , Victor I. Lyamichev , Mary Ann D. Brow , James E. Dahlberg
发明人： James R. Prudent , Jeff G. Hall , Victor I. Lyamichev , Mary Ann D. Brow , James E. Dahlberg
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译：本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。

7. 发明申请

US20090111092A1 DETERMINATION OF HEPATITIS C VIRUS GENOTYPE 有权
标题翻译： HEPATITIS C病毒基因组的测定
公开(公告)号：US20090111092A1
公开(公告)日：2009-04-30
申请号：US12346322
申请日：2008-12-30
申请人： Vecheslav A. Elagin , Scott M. Law , Bjork Hill
发明人： Vecheslav A. Elagin , Scott M. Law , Bjork Hill
IPC分类号： C12Q1/70
CPC分类号： C12Q1/6883 , C12Q2600/156
摘要： The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.
摘要翻译：本发明提供用于检测和表征HCV序列的组合物和方法。更具体地，本发明提供了用于使用侵入性切割结构测定（例如INVADER测定）来筛选例如来自患者的核酸样品以确定HCV基因型的组合物，方法和试剂盒。

8. 发明申请

US20090078574A1 CHARGE TAGS AND THE SEPARATION OF NUCLEIC ACID MOLECULES 失效
标题翻译：充电标签和核酸分子的分离
公开(公告)号：US20090078574A1
公开(公告)日：2009-03-26
申请号：US12132198
申请日：2008-06-03
申请人： Victor Lyamichev , Zbigniev Skrzypczynski , Hatim T. Allawi , Sarah R. Wayland , Tsetska Y. Takova , Bruce P. Neri , James R. Prudent , Jeff G. Hall
发明人： Victor Lyamichev , Zbigniev Skrzypczynski , Hatim T. Allawi , Sarah R. Wayland , Tsetska Y. Takova , Bruce P. Neri , James R. Prudent , Jeff G. Hall
IPC分类号： C25B7/00 , C07H21/04
CPC分类号： C12N9/22 , C07F9/2408 , C07F9/59 , C12Q1/6816 , C12Q1/6823 , C12Q1/683 , C12Q2525/197 , C12Q2565/125 , C12Q2563/107
摘要： The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.
摘要翻译：本发明涉及新型亚磷酰胺，包括正中和中性化合物。本发明还提供用于附着到包括固体支持物和核酸的材料的电荷标签，其中电荷标签增加或减少材料的净电荷。本发明还提供了基于修饰材料和未改性材料之间的电荷差异分离和表征分子的方法。

9. 发明申请

US20090068664A1 AMPLIFICATION METHODS AND COMPOSITIONS 有权
标题翻译：放大方法和组成
公开(公告)号：US20090068664A1
公开(公告)日：2009-03-12
申请号：US12174277
申请日：2008-07-16
申请人： Victor Lyamichev , Andrew Lukowiak , Nancy Jarvis , David Kurensky
发明人： Victor Lyamichev , Andrew Lukowiak , Nancy Jarvis , David Kurensky
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： G06Q30/06 , G06Q10/087
摘要： The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions.
摘要翻译：本发明提供用于开发和优化用于基础研究，临床研究和用于开发临床检测测定的核酸检测测定的方法和程序。特别地，本发明提供了用于多重扩增反应中使用的寡核苷酸引物的设计方法。本发明还提供优化多重扩增反应的方法。

10. 发明授权

US07473773B2 Determination of hepatitis C virus genotype 有权
标题翻译：丙型肝炎病毒基因型的测定
公开(公告)号：US07473773B2
公开(公告)日：2009-01-06
申请号：US11031487
申请日：2005-01-07
申请人： Vecheslav A. Elagin , Scott M. Law , Bjork Hill
发明人： Vecheslav A. Elagin , Scott M. Law , Bjork Hill
IPC分类号： C07H21/04
CPC分类号： C12Q1/6883 , C12Q2600/156
摘要： The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.
摘要翻译：本发明提供用于检测和表征HCV序列的组合物和方法。更具体地，本发明提供了用于使用侵入性切割结构测定（例如INVADER测定）来筛选例如来自患者的核酸样品以确定HCV基因型的组合物，方法和试剂盒。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式